BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0526.0012.p (129 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060865.1| COG1334: Uncharacterized flagellar protein Fl... 77 1e-13 ref|NP_622179.1| uncharacterized flagellar protein FlaG [Thermoa... 70 2e-11 ref|ZP_00169998.1| hypothetical protein Raeut123101 [Ralstonia e... 57 2e-07 ref|NP_693423.1| hypothetical protein OB2502 [Oceanobacillus ihe... 52 3e-06 >ref|ZP_00060865.1| COG1334: Uncharacterized flagellar protein FlaG [Clostridium thermocellum ATCC 27405] Length = 140 Score = 77.4 bits (189), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 34/70 (48%), Positives = 53/70 (75%) Query: 60 LIQQLEKAIRAVQGPEKTFKISIHEQTHSIMVKVFDKQTGDLIREIPPEKLLDVAANMME 119 +I+ +EKA +A+ G + SIHE++ IMVKV D +T ++IREIPPEK+LD+ A M+E Sbjct: 71 IIKAIEKANKAINGIHTELEFSIHEKSKEIMVKVIDSETKEVIREIPPEKILDMVAAMLE 130 Query: 120 LSGLIVDEKA 129 ++G+IV+E+ Sbjct: 131 MAGIIVNERG 140 >ref|NP_622179.1| uncharacterized flagellar protein FlaG [Thermoanaerobacter tengcongensis] gb|AAM23783.1| uncharacterized flagellar protein FlaG [Thermoanaerobacter tengcongensis] Length = 113 Score = 70.1 bits (170), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 32/77 (41%), Positives = 53/77 (68%) Query: 52 SLPVERDQLIQQLEKAIRAVQGPEKTFKISIHEQTHSIMVKVFDKQTGDLIREIPPEKLL 111 S PV+ D+L ++L K R + F+ S+H+ T++I+VK+ D +T ++I EIPPEK+L Sbjct: 36 SKPVDEDKLNRELNKENRRITIDRTYFEFSVHKPTNTIVVKIVDSETNEVIDEIPPEKIL 95 Query: 112 DVAANMMELSGLIVDEK 128 D+ A + +++GL VD K Sbjct: 96 DLVAGLWKIAGLFVDRK 112 >ref|ZP_00169998.1| hypothetical protein Raeut123101 [Ralstonia eutropha JMP134] Length = 112 Score = 56.6 bits (135), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 33/118 (27%), Positives = 64/118 (54%), Gaps = 16/118 (13%) Query: 16 VPSNTPA------AVQGVVFPTKGGSENDTSLKSSELAIETASLPVERDQLIQQLEKAIR 69 +PS+ PA A GV FP G ++D+ + +++T++ + + + Sbjct: 3 LPSDVPASTIGVQAPTGVSFPVVGNGQDDSGKTGAAGSVDTSA----------AVGELVE 52 Query: 70 AVQGPEKTFKISIHEQTHSIMVKVFDKQTGDLIREIPPEKLLDVAANMMELSGLIVDE 127 A++ + + I E TH ++ KV DK+TG+LIR++P ++L +A+ + +L GL V + Sbjct: 53 ALKSTSISLRFEIDESTHRVITKVVDKETGELIRQMPTVEVLRIASAIDKLQGLFVSQ 110 >ref|NP_693423.1| hypothetical protein OB2502 [Oceanobacillus iheyensis HTE831] dbj|BAC14458.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 120 Score = 52.4 bits (124), Expect = 3e-06, Method: Compositional matrix adjust. Identities = 35/117 (29%), Positives = 56/117 (47%), Gaps = 15/117 (12%) Query: 12 SNKIVPSNTPAAVQGVVFPTKGGSENDTSLKSSELAIETASLPVERDQLIQQLEKAIRAV 71 +NK+ S T + T+GG +E + L D + +L K IR Sbjct: 18 TNKVALSQTKSITNENSHQTQGGY--------AEKIVNKVDLEDAVDHM-NELLKPIR-- 66 Query: 72 QGPEKTFKISIHEQTHSIMVKVFDKQTGDLIREIPPEKLLDVAANMMELSGLIVDEK 128 + K +HE+ V V D +T ++I+EIPP+K+LD+ A + E G ++DEK Sbjct: 67 ----RNLKFEMHEKLERYYVTVVDSETSEVIKEIPPKKMLDMYAELAEFMGFLIDEK 119 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.129 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 175,709,108 Number of extensions: 5903658 Number of successful extensions: 15177 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 15180 Number of HSP's successfully gapped: 5 Length of query: 129 Length of database: 757,237,578 Length adjustment: 92 Effective length of query: 37 Effective length of database: 507,448,470 Effective search space: 18775593390 Effective search space used: 18775593390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 120 (50.8 bits)