BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0526.0011.p (241 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_622175.1| Flagellin and related hook-associated proteins ... 234 9e-61 ref|ZP_00183380.1| hypothetical protein Exigu123201 [Exiguobacte... 227 2e-58 gb|AAG15516.1| flagellin subunit FliC [Clostridium difficile] 224 1e-57 dbj|BAB87734.1| flagellin protein FliA(A) [Clostridium novyi] 221 1e-56 ref|NP_244483.1| flagellin [Bacillus halodurans] >gi|544311|sp|Q... 221 1e-56 >ref|NP_622175.1| Flagellin and related hook-associated proteins [Thermoanaerobacter tengcongensis] gb|AAM23779.1| Flagellin and related hook-associated proteins [Thermoanaerobacter tengcongensis] Length = 276 Score = 234 bits (598), Expect = 9e-61, Method: Compositional matrix adjust. Identities = 133/261 (50%), Positives = 166/261 (63%), Gaps = 20/261 (7%) Query: 1 MGLNTTATSKNMEKLSSGLRINRAADDAAGLSISESMRGQIRGLEQAQRNAQDGISFVQT 60 + +N T K +EKLSSG RINRA DDAAGL+ISE MRGQI GL QA RNAQDGIS +QT Sbjct: 16 LSINNFNTQKALEKLSSGYRINRAGDDAAGLAISEKMRGQINGLNQAIRNAQDGISLIQT 75 Query: 61 AEGAMNEVSSMLTRMKELNVQKASGTYSTNDKANIDKELDQLGDQIDEIMTKTTFN---- 116 AEGA+NE ++L RM+EL VQ A+ T + D+ NI KE++QL +ID I + T FN Sbjct: 76 AEGALNETHAILQRMRELVVQAANDTNTDVDRGNIQKEINQLKAEIDRIASTTQFNTRTL 135 Query: 117 --------GISITAGANITVNDKXXXXXXXXXXXXXDFRNLSSAT--------SLANVEV 160 G++ GAN N D ++S +T L ++ Sbjct: 136 LDGSLSTTGVTFQIGANAAQNMTLTIGKMDTTTLGVDTLDVSISTLSSSDIAAWLTTIDN 195 Query: 161 AIKSVSSERAKLGAVQNRLEYTSNNIGTTVENLSAAESRIRDTDMAKEMVALSKNNILLQ 220 AI VS +RA LGAVQNRLE+T NN+G ENL+AAESRIRD DMAKEM+ +KNNIL Q Sbjct: 196 AINKVSQQRADLGAVQNRLEHTINNLGVAAENLTAAESRIRDVDMAKEMMEFTKNNILQQ 255 Query: 221 ASQSMLAQANSAPQGILSVLR 241 A+ +MLAQAN PQ +L +LR Sbjct: 256 AATAMLAQANQQPQTVLQLLR 276 >ref|ZP_00183380.1| hypothetical protein Exigu123201 [Exiguobacterium sp. 255-15] Length = 249 Score = 227 bits (578), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 127/249 (51%), Positives = 164/249 (65%), Gaps = 19/249 (7%) Query: 12 MEKLSSGLRINRAADDAAGLSISESMRGQIRGLEQAQRNAQDGISFVQTAEGAMNEVSSM 71 MEKL+SGLRIN+A DDAAGL+ISE MRGQ+RGL+QA RNAQDGIS +QTAEGA+NE + Sbjct: 1 MEKLASGLRINKAGDDAAGLAISEKMRGQVRGLDQASRNAQDGISMIQTAEGALNETHDI 60 Query: 72 LTRMKELNVQKASGTYSTNDKANIDKELDQLGDQIDEIMTKTTFNGISITAGANITVNDK 131 L RM+EL+VQ ++GT +T D A I E D+L +ID I + T FNG + + A + V+ + Sbjct: 61 LQRMRELSVQASNGTNNTEDLAAIKAEGDELVTEIDRIASSTKFNGKDLISSAAVDVDLQ 120 Query: 132 XXXXXXXXXXXXXDFRNLSS-------------------ATSLANVEVAIKSVSSERAKL 172 LSS S A ++ AI VS+ R+ L Sbjct: 121 IGANDVASAAYEKLTLKLSSTKADTTTLLGATTLDVSAGGASTAKIDTAIGKVSTARSYL 180 Query: 173 GAVQNRLEYTSNNIGTTVENLSAAESRIRDTDMAKEMVALSKNNILLQASQSMLAQANSA 232 GA QNRLE+T NN+ T+ ENL+AAESRIRD DMAKEM+ +KN+IL QA+Q+MLAQ+N Sbjct: 181 GANQNRLEHTINNLKTSSENLTAAESRIRDVDMAKEMMNQTKNSILGQAAQAMLAQSNQT 240 Query: 233 PQGILSVLR 241 PQG+L +LR Sbjct: 241 PQGVLQLLR 249 >gb|AAG15516.1| flagellin subunit FliC [Clostridium difficile] Length = 281 Score = 224 bits (571), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 130/265 (49%), Positives = 170/265 (64%), Gaps = 25/265 (9%) Query: 1 MGLNTTATSKNMEKLSSGLRINRAADDAAGLSISESMRGQIRGLEQAQRNAQDGISFVQT 60 MG N + SK+MEKLSSGLRI RAADDAAGL+ISE MR Q++GL+QA RN QDGIS VQT Sbjct: 16 MGRNVSGQSKSMEKLSSGLRIKRAADDAAGLAISEKMRAQLKGLDQAGRNVQDGISVVQT 75 Query: 61 AEGAMNEVSSMLTRMKELNVQKASGTYSTNDKANIDKELDQLGDQIDEIMTKTTFNG--- 117 AEGA+ E ++LTRM+ L VQ ++ T S +++A I E++QL ++D I T FNG Sbjct: 76 AEGALEETGNILTRMRTLAVQASNETNSKDERAKIAGEMEQLRSEVDRIADSTKFNGENL 135 Query: 118 ------ISITAGANIT---------VNDKXXXXXXXXXXXXXDFRNLSSATS-------L 155 I++ GA +N K D ++S + + Sbjct: 136 LSSDKKIALQVGAEAVSNNVIEVSLINTKGVLTTRNVNSANIDAMSVSGSIGTEAASKMI 195 Query: 156 ANVEVAIKSVSSERAKLGAVQNRLEYTSNNIGTTVENLSAAESRIRDTDMAKEMVALSKN 215 N++ ++ ++S RA LGA QNRLE T NN+ TVEN++AAESRIRDTD+A EMV LSK Sbjct: 196 VNLDSSLADINSARALLGAQQNRLESTQNNLNNTVENVTAAESRIRDTDVASEMVNLSKM 255 Query: 216 NILLQASQSMLAQANSAPQGILSVL 240 NIL+QASQSML+QAN PQG+L +L Sbjct: 256 NILVQASQSMLSQANQQPQGVLQLL 280 >dbj|BAB87734.1| flagellin protein FliA(A) [Clostridium novyi] Length = 270 Score = 221 bits (563), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 135/255 (52%), Positives = 166/255 (65%), Gaps = 14/255 (5%) Query: 1 MGLNTTATSKNMEKLSSGLRINRAADDAAGLSISESMRGQIRGLEQAQRNAQDGISFVQT 60 MG N T K MEKLSSGLRINRA DDAAGL+ISE MRGQIRGL QA RNAQDGIS +QT Sbjct: 16 MGQNITGAGKAMEKLSSGLRINRAGDDAAGLAISEKMRGQIRGLNQASRNAQDGISLIQT 75 Query: 61 AEGAMNEVSSMLTRMKELNVQKASGTYSTNDKANIDKELDQLGDQIDEIMTKTTFN---- 116 AEGA+NE ++L RM+EL VQ ++ T +T D+ N+ KE+ QL ++I I T T FN Sbjct: 76 AEGALNETHAILQRMRELAVQTSNDTNTTADRTNVQKEIAQLKEEITRISTDTEFNTKKL 135 Query: 117 ------GISITAGAN----ITVNDKXXXXXXXXXXXXXDFRNLSSATSLANVEVAIKSVS 166 G++ GAN IT+N +S ++ + AI +VS Sbjct: 136 LNGSAKGVTFQVGANEKQIITLNIASMGTEDLGLKEVSLDTGAASQAAITKINDAINTVS 195 Query: 167 SERAKLGAVQNRLEYTSNNIGTTVENLSAAESRIRDTDMAKEMVALSKNNILLQASQSML 226 ERA LGA+QNRLE+T N+ ENL AAESR+RD DMAKEM+ SK NIL QA+Q+ML Sbjct: 196 GERATLGAIQNRLEHTINSDNNAAENLQAAESRVRDVDMAKEMMEFSKKNILQQAAQAML 255 Query: 227 AQANSAPQGILSVLR 241 AQAN PQG+L +LR Sbjct: 256 AQANQQPQGVLQLLR 270 >ref|NP_244483.1| flagellin [Bacillus halodurans] sp|Q05203|FLA_BACHD Flagellin pir||A47684 flagellin - Bacillus sp pir||H84101 flagellin hag [imported] - Bacillus halodurans (strain C-125) dbj|BAA00952.1| flagellin [Bacillus sp.] dbj|BAB07335.1| flagellin [Bacillus halodurans] Length = 272 Score = 221 bits (563), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 127/259 (49%), Positives = 162/259 (62%), Gaps = 20/259 (7%) Query: 1 MGLNTTATSKNMEKLSSGLRINRAADDAAGLSISESMRGQIRGLEQAQRNAQDGISFVQT 60 MG+N K MEKLSSGLRINRA DDAAGL+ISE MR QIRGL+QA RN+QDGIS +QT Sbjct: 16 MGINLNQGQKAMEKLSSGLRINRAGDDAAGLAISEKMRAQIRGLDQASRNSQDGISLIQT 75 Query: 61 AEGAMNEVSSMLTRMKELNVQKASGTYSTNDKANIDKELDQLGDQIDEIMTKTTFN---- 116 AEGA++EV S+L RM+EL VQ ++ T D+A ++ E QL ++I+ I T FN Sbjct: 76 AEGALDEVHSILQRMRELAVQSSNETNVEQDQAALNDEFQQLVEEIERIKDTTQFNTQKL 135 Query: 117 ------GISITAGAN--------ITVNDKXXXXXXXXXXXXXDFRNLSSATSLANVEVAI 162 + + GAN +T D D N SA + ++ + Sbjct: 136 LDDTVDTVQLQVGANSGELIELDLTKVDLSAIHTALAAEDITDHTNAQSA--IDAIDEQL 193 Query: 163 KSVSSERAKLGAVQNRLEYTSNNIGTTVENLSAAESRIRDTDMAKEMVALSKNNILLQAS 222 K+VS R+ LGA+QNRLE+T N+ ENL AAESRIRD DMAKEM+ ++ NIL QAS Sbjct: 194 KAVSEGRSYLGAMQNRLEHTIKNLDNASENLQAAESRIRDVDMAKEMMEFTRTNILNQAS 253 Query: 223 QSMLAQANSAPQGILSVLR 241 Q+MLAQAN PQ +L +LR Sbjct: 254 QAMLAQANQQPQAVLQLLR 272 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.307 0.121 0.302 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 242,479,756 Number of extensions: 7633926 Number of successful extensions: 28746 Number of sequences better than 1.0e-05: 1241 Number of HSP's gapped: 28450 Number of HSP's successfully gapped: 1786 Length of query: 241 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 119 Effective length of database: 425,995,500 Effective search space: 50693464500 Effective search space used: 50693464500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 124 (52.4 bits)