BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0044.p (303 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_981505.1| UDP-N-acetylenolpyruvoylglucosamine reductase [... 339 5e-92 ref|NP_653534.1| MurB, UDP-N-acetylenolpyruvoylglucosamine reduc... 337 2e-91 ref|NP_834731.1| UDP-N-acetylenolpyruvoylglucosamine reductase [... 335 1e-90 ref|ZP_00183425.1| hypothetical protein Exigu120201 [Exiguobacte... 328 7e-89 ref|NP_691477.1| UDP-N-acetylenolpyruvoylglucosamine reductase [... 323 3e-87 >ref|NP_981505.1| UDP-N-acetylenolpyruvoylglucosamine reductase [Bacillus cereus ATCC 10987] gb|AAS44113.1| UDP-N-acetylenolpyruvoylglucosamine reductase [Bacillus cereus ATCC 10987] Length = 305 Score = 339 bits (870), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 167/289 (57%), Positives = 207/289 (71%) Query: 1 MNISKIYEDLQQLVPTGMVKRGEVLESHVFTRIGGRADILAAPATYEELQSIVTYARVNE 60 MN+ ++YE L ++P G VK+ E+L++H ++GG+AD+ AP Y+E+Q ++ YA Sbjct: 1 MNMQEVYEYLSTVLPEGHVKQDEMLKNHTHIKVGGKADVFVAPTNYDEIQEVIKYANKYN 60 Query: 61 IPLTVLGNGSNVXXXXXXXXXXVLQTSDLTEMGLRDNVLYAQCGAKIIDVSRFALEKQLA 120 IP+T LGNGSNV + +T + + + AQCGA IIDVSR ALE L Sbjct: 61 IPVTFLGNGSNVIIKDGGIRGITVSLIHITGVTVTGTTIVAQCGAAIIDVSRIALEHNLT 120 Query: 121 GLEFACGIPGTVGGALYMNAGAYGGEIKDVLESALAISKAGRIVTLQGEELEWGYRKSVF 180 GLEFACGIPG+VGGALYMNAGAYGGEI VL A+ ++ G + TL E E+GYRKSVF Sbjct: 121 GLEFACGIPGSVGGALYMNAGAYGGEISYVLTEAVVMTGDGELRTLTKEAFEFGYRKSVF 180 Query: 181 ASSEFIVLEARFALQPGDPAAIKAAMDDLTHQRESKQPLEYPSCGSVFKRPPGRFAGQLI 240 A++ +I+LEARF L+ G IKA MDDLT +RESKQPLEYPSCGSVFKRPP FAG+LI Sbjct: 181 ANNHYIILEARFELEEGVYEEIKAKMDDLTFKRESKQPLEYPSCGSVFKRPPNNFAGKLI 240 Query: 241 QESGLQGTRIGGAEVSRKHAGFIVNTDHATAKDYIGLIQHVQATVKNKF 289 QESGLQG RIGG EVS KHAGF+VN D+ TA+DYI LI VQ TV+ KF Sbjct: 241 QESGLQGKRIGGVEVSLKHAGFMVNVDNGTAQDYIDLIHFVQKTVEEKF 289 >ref|NP_653534.1| MurB, UDP-N-acetylenolpyruvoylglucosamine reductase, FAD-binding domain [Bacillus anthracis A2012] ref|NP_847491.1| UDP-N-acetylenolpyruvoylglucosamine reductase [Bacillus anthracis str. Ames] gb|AAP28977.1| UDP-N-acetylenolpyruvoylglucosamine reductase [Bacillus anthracis str. Ames] Length = 305 Score = 337 bits (865), Expect = 2e-91, Method: Compositional matrix adjust. Identities = 166/289 (57%), Positives = 207/289 (71%) Query: 1 MNISKIYEDLQQLVPTGMVKRGEVLESHVFTRIGGRADILAAPATYEELQSIVTYARVNE 60 MN+ ++YE L ++P G VK+ E+L++H ++GG+AD+ AP Y+E+Q ++ YA Sbjct: 1 MNMQEVYEYLSTVLPEGHVKQDEMLKNHTHIKVGGKADVFVAPTNYDEIQEVIKYANKYN 60 Query: 61 IPLTVLGNGSNVXXXXXXXXXXVLQTSDLTEMGLRDNVLYAQCGAKIIDVSRFALEKQLA 120 IP+T LGNGSNV + +T + + + AQCGA IIDVSR AL+ L Sbjct: 61 IPVTFLGNGSNVIIKDGGIRGITVSLIHITGVTVTGTTIVAQCGAAIIDVSRIALDHNLT 120 Query: 121 GLEFACGIPGTVGGALYMNAGAYGGEIKDVLESALAISKAGRIVTLQGEELEWGYRKSVF 180 GLEFACGIPG+VGGALYMNAGAYGGEI VL A+ ++ G + TL E E+GYRKSVF Sbjct: 121 GLEFACGIPGSVGGALYMNAGAYGGEISFVLTEAVVMTGDGELRTLTKEAFEFGYRKSVF 180 Query: 181 ASSEFIVLEARFALQPGDPAAIKAAMDDLTHQRESKQPLEYPSCGSVFKRPPGRFAGQLI 240 A++ +I+LEARF L+ G IKA MDDLT +RESKQPLEYPSCGSVFKRPP FAG+LI Sbjct: 181 ANNHYIILEARFELEEGVYEEIKAKMDDLTFKRESKQPLEYPSCGSVFKRPPNNFAGKLI 240 Query: 241 QESGLQGTRIGGAEVSRKHAGFIVNTDHATAKDYIGLIQHVQATVKNKF 289 QESGLQG RIGG EVS KHAGF+VN D+ TA+DYI LI VQ TV+ KF Sbjct: 241 QESGLQGKRIGGVEVSLKHAGFMVNVDNGTAQDYIDLIHFVQKTVEEKF 289 >ref|NP_834731.1| UDP-N-acetylenolpyruvoylglucosamine reductase [Bacillus cereus ATCC 14579] gb|AAP11932.1| UDP-N-acetylenolpyruvoylglucosamine reductase [Bacillus cereus ATCC 14579] Length = 305 Score = 335 bits (858), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 164/289 (56%), Positives = 207/289 (71%) Query: 1 MNISKIYEDLQQLVPTGMVKRGEVLESHVFTRIGGRADILAAPATYEELQSIVTYARVNE 60 MN+ ++Y+ L ++P G VK+ E+L++H ++GG+AD+ AP Y+E+Q ++ YA Sbjct: 1 MNMQEVYKYLSTVLPEGHVKQDEMLKNHTHIKVGGKADVFVAPTNYDEIQEVIKYANEYN 60 Query: 61 IPLTVLGNGSNVXXXXXXXXXXVLQTSDLTEMGLRDNVLYAQCGAKIIDVSRFALEKQLA 120 IP+T LGNGSNV + +T + + + AQCGA IIDVSR AL+ L Sbjct: 61 IPVTFLGNGSNVIIKDGGIRGITVSLIHITGVTVTGTTIVAQCGAAIIDVSRIALDHNLT 120 Query: 121 GLEFACGIPGTVGGALYMNAGAYGGEIKDVLESALAISKAGRIVTLQGEELEWGYRKSVF 180 GLEFACGIPG+VGGALYMNAGAYGGEI VL A+ ++ G + TL E E+GYRKSVF Sbjct: 121 GLEFACGIPGSVGGALYMNAGAYGGEISFVLTEAVVMTGDGELRTLTKEAFEFGYRKSVF 180 Query: 181 ASSEFIVLEARFALQPGDPAAIKAAMDDLTHQRESKQPLEYPSCGSVFKRPPGRFAGQLI 240 A++ +I+LEARF L+ G IKA MDDLT +RESKQPLEYPSCGSVFKRPP FAG+LI Sbjct: 181 ANNHYIILEARFELEEGVHEEIKAKMDDLTFKRESKQPLEYPSCGSVFKRPPNNFAGKLI 240 Query: 241 QESGLQGTRIGGAEVSRKHAGFIVNTDHATAKDYIGLIQHVQATVKNKF 289 Q+SGLQG RIGG EVS KHAGF+VN D+ TA+DYI LI VQ TV+ KF Sbjct: 241 QDSGLQGKRIGGVEVSLKHAGFMVNVDNGTAQDYIDLIHFVQKTVEEKF 289 >ref|ZP_00183425.1| hypothetical protein Exigu120201 [Exiguobacterium sp. 255-15] Length = 304 Score = 328 bits (842), Expect = 7e-89, Method: Compositional matrix adjust. Identities = 159/267 (59%), Positives = 196/267 (73%) Query: 23 EVLESHVFTRIGGRADILAAPATYEELQSIVTYARVNEIPLTVLGNGSNVXXXXXXXXXX 82 E L++H +T++GG AD+ P+TYEE V YA +++PLT+LGNGSN+ Sbjct: 23 EPLKNHTYTKMGGLADLFLIPSTYEETAFAVRYAYEHDLPLTMLGNGSNLVVRDGGIRGI 82 Query: 83 VLQTSDLTEMGLRDNVLYAQCGAKIIDVSRFALEKQLAGLEFACGIPGTVGGALYMNAGA 142 VL LT++ + + L AQ GA II SR A + L+GLEFACGIPGT+GGAL MNAGA Sbjct: 83 VLSFEKLTDISVEGHELIAQSGAAIIQASRIAYDHALSGLEFACGIPGTIGGALIMNAGA 142 Query: 143 YGGEIKDVLESALAISKAGRIVTLQGEELEWGYRKSVFASSEFIVLEARFALQPGDPAAI 202 YGGE+KD L SA +++ G ++ + EELE GYR S F+ E+I+LE RF+L GDPA I Sbjct: 143 YGGEVKDCLHSATVLTRKGELLNISHEELELGYRTSCFSKKEYIILEGRFSLTEGDPALI 202 Query: 203 KAAMDDLTHQRESKQPLEYPSCGSVFKRPPGRFAGQLIQESGLQGTRIGGAEVSRKHAGF 262 K MDDLTH+RE+KQPLEYPSCGSVFKRP G FAG+LIQ+SGLQG RIGGAEVS+KHAGF Sbjct: 203 KEMMDDLTHKRETKQPLEYPSCGSVFKRPEGYFAGKLIQDSGLQGARIGGAEVSQKHAGF 262 Query: 263 IVNTDHATAKDYIGLIQHVQATVKNKF 289 IVN ATA DYI LI+HVQ TV+ KF Sbjct: 263 IVNIKDATATDYISLIRHVQETVQEKF 289 >ref|NP_691477.1| UDP-N-acetylenolpyruvoylglucosamine reductase [Oceanobacillus iheyensis HTE831] sp|Q8ESR4|MURB_OCEIH UDP-N-acetylenolpyruvoylglucosamine reductase (UDP-N-acetylmuramate dehydrogenase) dbj|BAC12512.1| UDP-N-acetylenolpyruvoylglucosamine reductase [Oceanobacillus iheyensis HTE831] Length = 307 Score = 323 bits (828), Expect = 3e-87, Method: Compositional matrix adjust. Identities = 156/284 (54%), Positives = 202/284 (71%) Query: 6 IYEDLQQLVPTGMVKRGEVLESHVFTRIGGRADILAAPATYEELQSIVTYARVNEIPLTV 65 +Y+ L ++ V E++ +H +T++GG+AD+ P +Y E+Q I+ A ++P T+ Sbjct: 7 LYDQLSRITSEENVMVDELIRNHTYTKLGGKADVYITPESYSEVQDIIKLANKEDVPFTM 66 Query: 66 LGNGSNVXXXXXXXXXXVLQTSDLTEMGLRDNVLYAQCGAKIIDVSRFALEKQLAGLEFA 125 LGNGSN+ V+ L + ++ + AQ GA+IID SR AL + LAGLEFA Sbjct: 67 LGNGSNLIVKDGGIRGIVMNLQKLASIWREEDKIIAQSGARIIDASRTALAESLAGLEFA 126 Query: 126 CGIPGTVGGALYMNAGAYGGEIKDVLESALAISKAGRIVTLQGEELEWGYRKSVFASSEF 185 CGIPG+VGGALYMNAGAYGGEIKDVLES + ++K G I TL EL+ YR S + Sbjct: 127 CGIPGSVGGALYMNAGAYGGEIKDVLESTIVVTKEGDIRTLTAVELDLDYRTSNIPDKGY 186 Query: 186 IVLEARFALQPGDPAAIKAAMDDLTHQRESKQPLEYPSCGSVFKRPPGRFAGQLIQESGL 245 IVLEA FAL+ + + IK MDDLT++RESKQPLEYPSCGSVFKRPPG FAG+LIQ+S L Sbjct: 187 IVLEATFALKKANSSDIKEVMDDLTYKRESKQPLEYPSCGSVFKRPPGYFAGKLIQDSEL 246 Query: 246 QGTRIGGAEVSRKHAGFIVNTDHATAKDYIGLIQHVQATVKNKF 289 QGT+IGGAEVS+KHAGFIVN ++A+A +YI LI+HVQ TV KF Sbjct: 247 QGTQIGGAEVSKKHAGFIVNKNNASATEYINLIRHVQKTVSEKF 290 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 389,359,595 Number of extensions: 14809561 Number of successful extensions: 34318 Number of sequences better than 1.0e-05: 546 Number of HSP's gapped: 33697 Number of HSP's successfully gapped: 547 Length of query: 303 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 178 Effective length of database: 417,850,203 Effective search space: 74377336134 Effective search space used: 74377336134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)