BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0021.p (442 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAN10244.1| valanimycin resistance [Streptomyces viridifaciens] 161 2e-38 ref|NP_823779.1| putative multi-drug efflux transporter [Strepto... 112 1e-23 ref|ZP_00032098.1| COG0477: Permeases of the major facilitator s... 104 3e-21 ref|ZP_00000103.1| COG0477: Permeases of the major facilitator s... 96 2e-18 gb|EAK43754.1| unknown [environmental sequence] 88 4e-16 >gb|AAN10244.1| valanimycin resistance [Streptomyces viridifaciens] Length = 402 Score = 161 bits (408), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 106/335 (31%), Positives = 165/335 (49%), Gaps = 3/335 (0%) Query: 87 YWAVAYGLFVTILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDR 146 +WA AY L + G NL PLY Y ++ SP IPS+++ G +SD Sbjct: 22 FWATAYTLLFLLTGTNLPTPLYRGYEHKFGFSPLVVTLIFAVYVASLIPSLLIAGPLSDS 81 Query: 147 IGPVRILLCGIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANS 206 IG ++LL + GSL F A+ + L +AR+LQGLAVG +G +A++EL + Sbjct: 82 IGRRKVLLPAVLLAAVGSLVFALADNVGWLFVARLLQGLAVGAASGALTAALSELEPSGD 141 Query: 207 KRQAAYLAALLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLVRIRSE 266 +R+AA ++ + G GP+++G+LAEY P P +PF+V ++L+VPA I + + S Sbjct: 142 RRRAALVSTVASVGGLGAGPLIAGLLAEYAPSPYVLPFVVEIVLLVPAAIAMATLP-TSR 200 Query: 267 PAGTASLHWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVDRTXXXXXXXX 326 PA P +PA +RG F ++ SFL + V+ LF+S+VPTY+ + Sbjct: 201 PATKWRPRRPSIPATMRGVFVSSGTASFLAFSVIGLFLSLVPTYVTKLSGSSNLALAGGA 260 Query: 327 XXXXXXXXXXXQFTL-GKLPLLRMMTXXXXXXXXXXXXXXXXXRFHSLIILFASILLVGM 385 Q G+ PL +T SL +L A+ ++ G+ Sbjct: 261 VALMLACSVIAQIAASGRQPLAIQITGLVLLAVGLVLLAVAGGV-GSLALLLAATVIGGV 319 Query: 386 GHGPLYAGSLAYVNAIAPSEARGDTVSFFYTGTYI 420 G G + G + +N +AP E D +S FY Y+ Sbjct: 320 GQGLAFLGGITEINRVAPKERHADVLSSFYVVIYL 354 >ref|NP_823779.1| putative multi-drug efflux transporter [Streptomyces avermitilis MA-4680] dbj|BAC70314.1| putative multi-drug efflux transporter [Streptomyces avermitilis MA-4680] Length = 411 Score = 112 bits (281), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 88/331 (26%), Positives = 147/331 (44%), Gaps = 3/331 (0%) Query: 96 VTILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDRIGPVRILLC 155 V ++G L PLY +Y+ ++ S I ++L G SD +G +LL Sbjct: 35 VCMVGTTLPTPLYGLYQEKFGFSELTVTVVYAVYAFGVIGMLLLAGNASDAVGRRPVLLA 94 Query: 156 GIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANSKRQAAYLAA 215 G+ ++CFL A GL L R+L GL+ G+F G A + +L +A ++A Sbjct: 95 GLGFAAVSAVCFLCATGLGWLYAGRLLSGLSAGLFTGAATVYVIDLAPYGGASRATFVAT 154 Query: 216 LLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLVV--PAVIGLLLVRIRS-EPAGTAS 272 G GP+L+G+LA+Y WP +PF+VHL LV AV+ L +R P T Sbjct: 155 AANMGGLGCGPLLAGVLAQYAAWPLYLPFVVHLALVAGSAAVLLWLPETVREPRPLSTVR 214 Query: 273 LHWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVDRTXXXXXXXXXXXXXX 332 P +P+ +R F A+ SF+ + + +F SV P +L + Sbjct: 215 PQRPALPSQVRAVFVPAATASFVGFALFGVFTSVSPAFLEQSLGVDNRAVVGLIVALAFF 274 Query: 333 XXXXXQFTLGKLPLLRMMTXXXXXXXXXXXXXXXXXRFHSLIILFASILLVGMGHGPLYA 392 Q +G++ + R + R+ L ++ S L+ G G G + Sbjct: 275 ASTAGQLMVGRVGVGRSLPLGCAGLFAGLALLAGALRWDLLSLVVLSALVGGGGQGLAFR 334 Query: 393 GSLAYVNAIAPSEARGDTVSFFYTGTYIGVA 423 G+L+ V +P++ R +S + Y+G++ Sbjct: 335 GALSAVAEASPADRRAAVISTLFVVAYVGIS 365 >ref|ZP_00032098.1| COG0477: Permeases of the major facilitator superfamily [Burkholderia fungorum] Length = 407 Score = 104 bits (260), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 69/220 (31%), Positives = 113/220 (51%), Gaps = 2/220 (0%) Query: 96 VTILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDRIGPVRILLC 155 + + G L +Y Y+ Q+ +P + +++L G SD++G R+LL Sbjct: 23 INMTGTTLPTAIYRYYQLQFGFTPTVITVIYASYAIGVLSALLLIGNWSDQLGRRRMLLA 82 Query: 156 GIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANSKRQAAYLAA 215 G+ A+ + +L FL ++GL++L++ R+L G++ G+F G A A+ EL + +A A Sbjct: 83 GLCASAASALTFLLSDGLALLIVGRLLSGVSAGIFTGTATVAVIELAPPEWRSKAMLAAT 142 Query: 216 LLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLVRIRSEPAGTASLHW 275 G GP+LSG L E PWP ++P+ VHL LV AV+ + V + L Sbjct: 143 SSNMLGLGCGPLLSGFLVERFPWPMQLPYAVHLALVGIAVLAVAGVPETAAVPARVKLGI 202 Query: 276 PRV--PAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGV 313 ++ PA +R AF A+L F + VV F +V P + V Sbjct: 203 QKISLPAEVRRAFIPAALAGFAGFVVVGFFAAVAPQLIRV 242 >ref|ZP_00000103.1| COG0477: Permeases of the major facilitator superfamily [Ferroplasma acidarmanus] Length = 402 Score = 95.5 bits (236), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 81/343 (23%), Positives = 151/343 (44%), Gaps = 17/343 (4%) Query: 92 YGLFVTILGL------NLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSD 145 YGL V +L N+ PLY++Y +++ IPS++ +GQ SD Sbjct: 17 YGLIVALLIFAIMVVSNIPTPLYALYAIKFNFGSLAIAGIFAIYVVFLIPSLLFWGQYSD 76 Query: 146 RIGPVRILLCGIAATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKAN 205 G ++ GI ++G + FL A +S L LAR QGLA G +G A SA+ ++ Sbjct: 77 VKGGKIPIILGIIFEIAGIIAFLLAGNISELFLARAFQGLASGAISGPA-SALLFHYRG- 134 Query: 206 SKRQAAYLAALLVTAGNALGPVLSGILAEYTPWPTRMPFLVHLLLV------VPAVIGLL 259 + A L ++ ++G A+GP+L G++AEY P+ ++ +++ L+++ + +I Sbjct: 135 --KAGAILTSVSTSSGTAVGPLLGGVMAEYLPFHLKLVYILSLIIIIIPVARIAPMINKS 192 Query: 260 LVRIRSEPAGTASLHWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYLGVWVDRTX 319 +V+ R H+P++ + F ++ T+F+ W + + +MS+ P Y+ + + Sbjct: 193 IVK-RQSNHNVFEFHFPKLDMEEKNLFTLSAATAFIAWSITAFYMSMSPLYIIRLLGISN 251 Query: 320 XXXXXXXXXXXXXXXXXXQFTLGKLPLLRMMTXXXXXXXXXXXXXXXXXRFHSLIILFAS 379 Q K + + F S+ I + Sbjct: 252 IAITGLIVFLMLGIASIMQILSMKSTIFSSLLTGIISLIIAIALIVISFPFKSMDIFIVA 311 Query: 380 ILLVGMGHGPLYAGSLAYVNAIAPSEARGDTVSFFYTGTYIGV 422 ++ G+G G + G+ + I+P G +S +Y Y GV Sbjct: 312 TIMTGIGQGFAFTGATREIRTISPPLKMGGVLSNYYIIIYFGV 354 >gb|EAK43754.1| unknown [environmental sequence] Length = 381 Score = 87.8 bits (216), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 74/220 (33%), Positives = 116/220 (52%), Gaps = 10/220 (4%) Query: 98 ILGLNLAAPLYSVYRTQWDLSPXXXXXXXXXXXXXXIPSIVLFGQVSDRIGPVRILLCGI 157 ++G L +Y Y+ Q+ SP + +++L G SD++G R+LL G+ Sbjct: 1 MMGTTLPTSIYRYYQLQFGFSPTVITVIYASYAAGVLGALLLIGNWSDQLGRRRMLLAGL 60 Query: 158 AATLSGSLCFLFANGLSMLLLARVLQGLAVGMFNGVAVSAMTELHKANSKRQAAYLAALL 217 A+ + +L FL ++GL++L+L R+L G++ G+F G A A+ EL + R A LAA Sbjct: 61 CASAASALTFLLSDGLALLMLGRLLSGISAGIFTGTATVAVIELAP-PAWRGKAMLAA-- 117 Query: 218 VTAGNALG----PVLSGILAEYTPWPTRMPFLVHLLLVVPAVIGLLLV--RIRSEPAGTA 271 TA N LG P+LSGIL E P ++P++VHL LV A + + V + P Sbjct: 118 -TASNMLGLGCGPLLSGILVELFRRPMQLPYVVHLALVGVAALAVFSVPETATTPPRVKL 176 Query: 272 SLHWPRVPAGIRGAFYTASLTSFLVWGVVSLFMSVVPTYL 311 + +PA +R AF A+L F + VV F +V P + Sbjct: 177 GIQKISLPAEVRRAFIPAALAGFAGFVVVGFFAAVAPQLI 216 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.139 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 518,490,431 Number of extensions: 18582375 Number of successful extensions: 63168 Number of sequences better than 1.0e-05: 144 Number of HSP's gapped: 63739 Number of HSP's successfully gapped: 144 Length of query: 442 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 313 Effective length of database: 406,989,807 Effective search space: 127387809591 Effective search space used: 127387809591 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)