BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0017.p (452 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_217376.1| glnA4 [Mycobacterium tuberculosis H37Rv] >gi|15... 373 e-102 ref|NP_961865.1| GlnA4 [Mycobacterium avium subsp. paratuberculo... 348 1e-94 ref|NP_827901.1| putative glutamine synthetase [Streptomyces ave... 342 1e-92 ref|NP_699909.1| glutamine synthetase family protein [Brucella s... 339 7e-92 gb|EAI73972.1| unknown [environmental sequence] 338 1e-91 >ref|NP_217376.1| glnA4 [Mycobacterium tuberculosis H37Rv] ref|NP_337439.1| glutamine synthetase, putative [Mycobacterium tuberculosis CDC1551] ref|NP_856530.1| PROBABLE GLUTAMINE SYNTHETASE GLNA4 (GLUTAMINE SYNTHASE) (GS-II) [Mycobacterium bovis subsp. bovis AF2122/97] pir||F70885 probable glnA4 protein - Mycobacterium tuberculosis (strain H37RV) emb|CAA15522.1| PROBABLE GLUTAMINE SYNTHETASE GLNA4 (GLUTAMINE SYNTHASE) (GS-II) [Mycobacterium tuberculosis H37Rv] gb|AAK47253.1| glutamine synthetase, putative [Mycobacterium tuberculosis CDC1551] emb|CAD96572.1| PROBABLE GLUTAMINE SYNTHETASE GLNA4 (GLUTAMINE SYNTHASE) (GS-II) [Mycobacterium bovis subsp. bovis AF2122/97] Length = 457 Score = 373 bits (958), Expect = e-102, Method: Compositional matrix adjust. Identities = 202/450 (44%), Positives = 273/450 (60%), Gaps = 6/450 (1%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIED-AKDGIHTCALNLAWDVS 62 L W +L R V +G+V +VI+A DMQG+L GKR+ G HF++D A G+ C+ LA DV Sbjct: 9 LAWTELERLVAAGDVDTVIVAFTDMQGRLAGKRISGRHFVDDIATRGVECCSYLLAVDVD 68 Query: 63 LN-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNML 121 LN + +WDTG DMV PDLSTLR PW P TA V++DL DG++++++PR++L Sbjct: 69 LNTVPGYAMASWDTGYGDMVMTPDLSTLRLIPWLPGTALVIADLVWADGSEVAVSPRSIL 128 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 +RQ+ + GL A+E+EF +F + Y GL P +Y +DY+I + Sbjct: 129 RRQLDRLKARGLVADVATELEFIVFDQPYRQAWASGYRGLTPASDYNIDYAILASSRMEP 188 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 L + + AG EA+KGE GQ E+ RY EAL D AIYKNG KE+A +G Sbjct: 189 LLRDIRLGMAGAGLRFEAVKGECNMGQQEIGFRYDEALVTCDNHAIYKNGAKEIADQHGK 248 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 TFMAK D + G+S H H+SL +G+ V D +G+S R F+AG L E Sbjct: 249 SLTFMAKYDERE-GNSCHIHVSLRG-TDGSAVFADSNGPHGMSSMFRSFVAGQLATLREF 306 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYL 361 + YAP INSYKR D S AP ++WG+DNR+ + R G G++ R E RVPG D N YL Sbjct: 307 TLCYAPTINSYKRFAD-SSFAPTALAWGLDNRTCALRVVGHGQNIRVECRVPGGDVNQYL 365 Query: 362 VLSAMIASGLYGIENRLELPAPITGNAGK-LEIPRLPTNLYDAVKILENSKTVRELLGEE 420 ++A+IA GLYGIE L+LP P GNA + ++ RLP L DA + E+S VRE GE+ Sbjct: 366 AVAALIAGGLYGIERGLQLPEPCVGNAYQGADVERLPVTLADAAVLFEDSALVREAFGED 425 Query: 421 VVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 VV HYL A++EL F ++VTDWER + FE Sbjct: 426 VVAHYLNNARVELAAFNAAVTDWERIRGFE 455 >ref|NP_961865.1| GlnA4 [Mycobacterium avium subsp. paratuberculosis str. k10] gb|AAS05248.1| GlnA4 [Mycobacterium avium subsp. paratuberculosis str. k10] Length = 461 Score = 348 bits (894), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 194/456 (42%), Positives = 263/456 (57%), Gaps = 10/456 (2%) Query: 2 AYLEWNKLRRQVESG----EVTSVIIAGVDMQGKLFGKRVPGHHFI-EDAKDGIHTCALN 56 A L L R V +G V +VI+A DMQG+L GKR+ F+ E A G+ C Sbjct: 7 AMLSLAALDRLVAAGAPETRVDTVIVAFPDMQGRLVGKRMDARLFVDEAAATGVECCGYL 66 Query: 57 LAWDVSLN-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISI 115 LA DV +N G + WDTG D+V PDLSTLR PW P TA V++D+ DG+ +++ Sbjct: 67 LAVDVDMNTVGGYAISGWDTGYGDLVMRPDLSTLRRIPWLPGTALVIADVVGADGSPVAV 126 Query: 116 APRNMLKRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYR 175 +PR +L+RQ+ + AG GL AA+E+EF +F E Y GL P +Y +DY+I Sbjct: 127 SPRAVLRRQLDRLAGRGLFADAATELEFMVFDEPYRQAWASGYRGLTPASDYNIDYAISA 186 Query: 176 LTVDHWFLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEM 235 + L + + AG E++KGE GQ E+ RY EAL D IYKNG KE+ Sbjct: 187 SSRMEPLLRDIRRGMAGAGLRFESVKGECNRGQQEIGFRYDEALRTCDNHVIYKNGAKEI 246 Query: 236 AALNGLMATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGML 295 A +G TFMAK D + G+S H+SL D + G DP + +G+S FLAG+L Sbjct: 247 ADQHGKSLTFMAKYDERE-GNSCRVHLSLRDAQ-GGAAFADPSRPHGMSTMFCSFLAGLL 304 Query: 296 ELAPELMVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGA 355 + +FYAP INSYKR D S AP ++WG+DNR+ + R G G R E RVPG Sbjct: 305 ATMADFTLFYAPNINSYKRFAD-ESFAPTALAWGLDNRTCALRVVGHGAHTRVECRVPGG 363 Query: 356 DANHYLVLSAMIASGLYGIENRLELPAPITGNAGKLE-IPRLPTNLYDAVKILENSKTVR 414 D N YL ++A++A GLYGIE L LP P GNA + + RLP L +A + E+S R Sbjct: 364 DVNPYLAVAAIVAGGLYGIEQGLALPEPCAGNAYRARGVGRLPGTLAEAAALFEHSALAR 423 Query: 415 ELLGEEVVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 ++ G++VV HYL A++EL F ++ TDWER + FE Sbjct: 424 QVFGDDVVAHYLNNARVELAAFHAAATDWERMRGFE 459 >ref|NP_827901.1| putative glutamine synthetase [Streptomyces avermitilis MA-4680] dbj|BAC74436.1| putative glutamine synthetase [Streptomyces avermitilis MA-4680] Length = 454 Score = 342 bits (877), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 184/445 (41%), Positives = 265/445 (59%), Gaps = 6/445 (1%) Query: 8 KLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIEDA-KDGIHTCALNLAWDVSLN-F 65 +L V GE+ +V++A DMQG+L GKR F+++ + G C LA D +N Sbjct: 12 ELHALVAGGEIDTVVLAFPDMQGRLQGKRFAARFFLDEVLEHGTEGCNYLLAVDTDMNTV 71 Query: 66 GAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNMLKRQI 125 +D +WD G D PDLSTLR PW+ TA +++DL DG+ + APR +L+RQ+ Sbjct: 72 DGYDMSSWDRGYGDFAMHPDLSTLRQVPWNAGTALLIADLAWNDGSPVVAAPRQILRRQL 131 Query: 126 AKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHWFLGQ 185 + A LG +E+EF +FK+T E + Y GL P +Y +DYS+ L + Sbjct: 132 DRLAALGYTAQVGTELEFIVFKDTYEQAWDAGYKGLTPANQYNIDYSVLGTGRIEPLLRR 191 Query: 186 LTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGLMATF 245 + + ++AAG +E+ KGE GQ E+ RY EAL D+ A+YK G KE+AA G+ TF Sbjct: 192 IRNEMQAAGLTVESAKGECNPGQHEIVFRYDEALVTCDQHAVYKTGAKEIAAQEGVSITF 251 Query: 246 MAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPELMVFY 305 MAK + + G+S H H+SL D + N + D G+S+ R+FLAG L + + Y Sbjct: 252 MAKYNERE-GNSCHIHLSLADADGTNAMAGDGPG--GMSDVMRHFLAGQLAALRDFSLLY 308 Query: 306 APYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYLVLSA 365 AP INSYKR GS AP V+WG DNR+ + R G G+S RFENR+PG D N +L ++ Sbjct: 309 APNINSYKRFQP-GSFAPTAVAWGYDNRTCALRVVGHGRSMRFENRLPGGDVNPHLAVAG 367 Query: 366 MIASGLYGIENRLELPAPITGNAGKLEIPRLPTNLYDAVKILENSKTVRELLGEEVVHHY 425 ++A+GLYGIE++LELP GNA E +PT L +A ++ ENS + G++VV HY Sbjct: 368 LVAAGLYGIEHKLELPEACAGNAYAAEYEHVPTTLREAAELWENSPIAKAAFGDDVVAHY 427 Query: 426 LQAAQLELRDFFSSVTDWERKKYFE 450 A++EL+ F ++VTDWE ++ FE Sbjct: 428 RNMARVELKAFDAAVTDWELRRSFE 452 >ref|NP_699909.1| glutamine synthetase family protein [Brucella suis 1330] gb|AAN33914.1| glutamine synthetase family protein [Brucella suis 1330] Length = 455 Score = 339 bits (870), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 185/450 (41%), Positives = 260/450 (57%), Gaps = 4/450 (0%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIEDAKDGIHTCALNLAWDVSL 63 L +++L++ V G + +V+ VDMQG+L GKR G F+E D H C LA D+ + Sbjct: 5 LTFDELKKAVAEGRIDTVLACIVDMQGRLIGKRFYGQFFVESGYDETHGCNYLLADDIDM 64 Query: 64 N-FGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISIAPRNMLK 122 ++ WD G D V PDLSTLR PW KTA VL D+ D+S +PR +LK Sbjct: 65 EPVPGYEAAGWDKGYGDFVMKPDLSTLRLAPWLEKTAIVLCDVLDHHHDDLSHSPRAVLK 124 Query: 123 RQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHWF 182 +Q+ + G + Y ASE+EFYIF ET ++ R K + ++ Y DY I+ T + Sbjct: 125 KQVQRLHERGYRAYFASELEFYIFDETYKSARAKRWHEMETASPYVQDYVIHLTTREEPV 184 Query: 183 LGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGLM 242 L + ++L AG +E KGEWG GQ ELNVRY +ALEMADR I KN +KE+A +G Sbjct: 185 LRAMRNHLADAGIPVENSKGEWGPGQQELNVRYCKALEMADRHVIMKNAMKEIAEAHGKC 244 Query: 243 ATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPELM 302 TFMAK D +GSS H H S+W + + +DP+ Y ++ R ++AG ++ A + Sbjct: 245 ITFMAKYDYARAGSSSHVHNSIWSADGKEPLFFDPKAPYTMTPLMRSWVAGQIKYATDYT 304 Query: 303 VFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKG-KSCRFENRVPGADANHYL 361 F APYINSYKR G+ AP + W DNR+ FR G+G K R E R+ GAD N YL Sbjct: 305 YFLAPYINSYKRFQA-GTFAPTKIMWSQDNRTAGFRLCGEGTKGIRIECRIGGADINPYL 363 Query: 362 VLSAMIASGLYGIENRLELPAPITGNA-GKLEIPRLPTNLYDAVKILENSKTVRELLGEE 420 +A+IA+GL G++ +LEL P G+A + + +P L +A L+ S ++E GE+ Sbjct: 364 AFAALIAAGLKGVDEKLELDEPFVGDAYSAVRLKEIPYTLREAAAALKGSAFLKEAFGED 423 Query: 421 VVHHYLQAAQLELRDFFSSVTDWERKKYFE 450 VV+HY A E ++ VTDWE + FE Sbjct: 424 VVNHYTHTAHWEQIEYDRRVTDWELYRGFE 453 >gb|EAI73972.1| unknown [environmental sequence] Length = 452 Score = 338 bits (868), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 174/449 (38%), Positives = 268/449 (59%), Gaps = 3/449 (0%) Query: 4 LEWNKLRRQVESGEVTSVIIAGVDMQGKLFGKRVPGHHFIEDAKDGIHTCALNLAWDVSL 63 + +N+L++ + E+ +V++ DMQG+L GKRV G FI+ H C D+ + Sbjct: 3 ITFNQLKKLITKKEIDTVLVCVSDMQGRLNGKRVTGKAFIDYVYKETHMCDYLYTVDMDM 62 Query: 64 -NFGAFDFCNWDTGVHDMVTLPDLSTLRPYPWSPKTAFVLSDLKSKDGTDISI-APRNML 121 + +W+TG DM +PDL T++ W KTA V+ D G I + R +L Sbjct: 63 FTVPGYKSSSWETGYGDMTVIPDLKTIKIANWLNKTAIVICDCLDHSGKKPLIHSTRQIL 122 Query: 122 KRQIAKAAGLGLKPYAASEIEFYIFKETSETVREKNYTGLKPLFEYPVDYSIYRLTVDHW 181 K +AKA +G + SE+EF++F +T E + NYT LK Y DY I++ T + Sbjct: 123 KDVLAKADKMGFQSMVGSELEFFLFNQTYEEIHNNNYTKLKESSWYIEDYMIFQTTKEED 182 Query: 182 FLGQLTDNLEAAGGAIEALKGEWGNGQIELNVRYAEALEMADRTAIYKNGIKEMAALNGL 241 + +L ++L +G +E KGE GQ E+NV +++AL MAD + KN KE+A + Sbjct: 183 VMQELRNSLLDSGIYVECSKGEAAPGQEEINVVFSDALNMADNHILIKNATKEIAYKHNK 242 Query: 242 MATFMAKQDTNDSGSSGHTHISLWDLENGNNVLYDPEQEYGLSETGRYFLAGMLELAPEL 301 TFMAK + GSS H H SL+D + N+ Y+P+ +YG+S+ + +LAG ++L P++ Sbjct: 243 AVTFMAKYNKEVCGSSCHIHNSLFDKKTKKNIFYNPKDKYGMSDIFKSYLAGQIKLLPDI 302 Query: 302 MVFYAPYINSYKRIMDFGSGAPNTVSWGIDNRSTSFRFDGKGKSCRFENRVPGADANHYL 361 +F AP INSYKR D GS AP WGIDNR+ FR G G S R E RVPGAD N YL Sbjct: 303 SIFLAPNINSYKRFQD-GSFAPTKSVWGIDNRTAGFRLAGHGSSIRIECRVPGADVNPYL 361 Query: 362 VLSAMIASGLYGIENRLELPAPITGNAGKLEIPRLPTNLYDAVKILENSKTVRELLGEEV 421 +A++ +GLYGI+N+L+L P +GN + ++ +P L +A+++ + SK ++E+ ++V Sbjct: 362 SFAALVGAGLYGIKNKLKLDKPYSGNIYEKKVNEVPKTLNEAIQLAKKSKFLKEIFPQDV 421 Query: 422 VHHYLQAAQLELRDFFSSVTDWERKKYFE 450 + HY+ AA+ E +D+ SV DW+ ++YFE Sbjct: 422 LDHYIHAAEWEQKDYDGSVNDWQLRRYFE 450 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 700,467,209 Number of extensions: 30242715 Number of successful extensions: 73277 Number of sequences better than 1.0e-05: 1521 Number of HSP's gapped: 69744 Number of HSP's successfully gapped: 1526 Length of query: 452 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 323 Effective length of database: 406,989,807 Effective search space: 131457707661 Effective search space used: 131457707661 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)