BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0523.0002.p (91 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_631192.1| putative lipase [Streptomyces coelicolor A3(2)]... 81 7e-15 ref|NP_617527.1| triacylglycerol lipase [Methanosarcina acetivor... 56 3e-07 >ref|NP_631192.1| putative lipase [Streptomyces coelicolor A3(2)] emb|CAC04237.1| putative lipase [Streptomyces coelicolor A3(2)] Length = 316 Score = 81.3 bits (199), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 43/87 (49%), Positives = 61/87 (70%), Gaps = 2/87 (2%) Query: 5 NEIILEAAARKFADDNAEPPFLPDLGPEKGRETVNTVQSSEIYKPEADLQDLTVPGGPAG 64 ++I+LE AA+ FAD A+PP L +LG E R+ ++ VQS + KP+ D + +TVP G Sbjct: 2 SDIVLEPAAQDFADATAKPPLLYELGVEGARKLLDDVQSGPVEKPDVDEKWITVP-VEVG 60 Query: 65 EVRVRIVRPVNTSGETLPVILYIHGAG 91 +VRVRIV+P T+G LPV+LY+HG G Sbjct: 61 DVRVRIVKPAGTTG-VLPVVLYVHGGG 86 >ref|NP_617527.1| triacylglycerol lipase [Methanosarcina acetivorans str. C2A] gb|AAM06007.1| triacylglycerol lipase [Methanosarcina acetivorans str. C2A] Length = 321 Score = 55.8 bits (133), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 31/85 (36%), Positives = 48/85 (56%), Gaps = 3/85 (3%) Query: 9 LEAAARKFADDNAEPPFLP--DLGPEKGRETVNTVQSSEIYKPEADLQDLTVPGGPAGEV 66 LE A R F + P L P+ + + +Q++E+ K AD+++LT+P G G+V Sbjct: 8 LEPATRIFVEKINRESDTPVYKLSPKDACKVLYDLQAAEVAKLPADIENLTIPVGSEGQV 67 Query: 67 RVRIVRPVNTSGETLPVILYIHGAG 91 +RI+R E LPV++Y HGAG Sbjct: 68 SIRIIRSAGNK-EILPVVMYFHGAG 91 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 147,397,824 Number of extensions: 5485436 Number of successful extensions: 9430 Number of sequences better than 1.0e-05: 2 Number of HSP's gapped: 9430 Number of HSP's successfully gapped: 2 Length of query: 91 Length of database: 757,237,578 Length adjustment: 59 Effective length of query: 32 Effective length of database: 597,046,737 Effective search space: 19105495584 Effective search space used: 19105495584 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 120 (50.8 bits)