BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0057.p (397 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00106576.1| COG1960: Acyl-CoA dehydrogenases [Nostoc punc... 191 3e-47 sp|P54998|SOXC_RHOSO Dibenzothiophene desulfurization enzyme C (... 174 4e-42 ref|ZP_00034886.1| COG1960: Acyl-CoA dehydrogenases [Burkholderi... 171 3e-41 gb|AAP80184.1| DszC [Rhodococcus erythropolis] 170 5e-41 gb|AAQ96171.1| dibenzothiophene desulfurization sulfur oxidation... 170 5e-41 >ref|ZP_00106576.1| COG1960: Acyl-CoA dehydrogenases [Nostoc punctiforme] Length = 383 Score = 191 bits (485), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 123/371 (33%), Positives = 183/371 (49%), Gaps = 21/371 (5%) Query: 33 SAKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGAGYRGDAVLLTAVLMELASWCSS 92 +A E D +G P + LR + L VP + GGLG + + + + E A SS Sbjct: 26 TAVERDAQGGTPKHERDRLRQSNLLKLIVPTEFGGLGQSW----ITVLQITREFAKVDSS 81 Query: 93 TSQIFALHNTGVQLVHALGTAEQQQFFFKEAVEGHLFASFGSEANKDRTALTSVLEHTDG 152 + +F+ H+ GV + H G+A+Q+Q ++ E + + F RT LT ++ Sbjct: 82 IAHVFSYHHLGVVIPHIFGSAQQKQRYYSETIHNNWFWCNALNPLDKRTTLTPENDY--- 138 Query: 153 GYLLNGKKIFATGSPGAKWAFWRSVSAEASGSQDERYMMPIVQLSADGVTVSDDWDGIGQ 212 + LNG K F +GS + + A+ + + ++ GVTV DDWD +GQ Sbjct: 139 -FRLNGIKSFCSGSQDSD-----ILPITATHQETNELNILVIPTQRQGVTVHDDWDNMGQ 192 Query: 213 RGTGSGRVEAVNVFV-PDGHVAGGPGAYSACSSFFAAQFHVNFAAQFVGMAAGAFREARQ 271 R T SG V NV V P+ + A ++ A +N A ++G+A G F A+ Sbjct: 193 RQTDSGSVTFENVLVYPEEILGSRNKANQPFNTIRACLTQLNLANIYLGIAQGVFEAAKT 252 Query: 272 YILEQARPW--QGAGRAAEDPFTLLRMGDMAVRIDSARQLVLRASRLLGACHEQPELLPE 329 Y +PW G A +DP+ L G + V + +A L +A LL A EQ L Sbjct: 253 YTSTNTKPWLTSGVESATQDPYILQHYGKIWVDLQAAVYLTEQAGELLQAAWEQEWSLTA 312 Query: 330 AQTA-----ASRAKVAATDAALEVTGSVFQVMGARAASRKYGIDMYYRNARTLTLHDPVD 384 Q A + AKV AT L++T +F+VMGARA S KYG D Y+RN RT TLHDPVD Sbjct: 313 EQRAECALLVATAKVFATKVGLDITNRIFEVMGARATSAKYGFDRYWRNLRTFTLHDPVD 372 Query: 385 RQREAVGRYEL 395 + + +G++ L Sbjct: 373 YKIQEIGKWAL 383 >sp|P54998|SOXC_RHOSO Dibenzothiophene desulfurization enzyme C (DBT sulfur dioxygenase) gb|AAA56673.1| dibenzothiophene desulfurization enzyme emb|CAD55892.3| sulfide-sulfoxide monooxygenase [Rhodococcus sp. FMF] emb|CAD56382.1| sulphide-sulphoxide monooxygenase [Rhodococcus sp. FMF] gb|AAP33510.1| dibenzothiophene desulfurization enzyme C [Rhodococcus sp. XP] prf||2119215C dibenzothiophene desulfurization enzyme Length = 417 Score = 174 bits (440), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 125/378 (33%), Positives = 179/378 (47%), Gaps = 20/378 (5%) Query: 33 SAKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGAGYRGDAVLLTAVLMELASWCSS 92 +A E DR G LR +G L VP + GG GA D V+ E+A+ S Sbjct: 34 TAVERDRAGGSATAEREDLRASGLLSLLVPREYGGWGA----DWPTAIEVVREIAAADGS 89 Query: 93 TSQIFALHNTGVQLVHALGTAEQQQFFFKEAVEGHLFASFGSEANKDRTA--LTSVLEHT 150 +F H T ++ +G+ EQ++ + + + + + S N S Sbjct: 90 LGHLFGYHLTNAPMIELIGSQEQEEHLYTQIAQNNWWTGNASSENNSHVLDWKVSATPTE 149 Query: 151 DGGYLLNGKKIFATGSPGAKWAFWRSVSAEASGSQDERYMMPIVQLSADGVTVSDDWDGI 210 DGGY+LNG K F +G+ G+ F V + S Q + + S GVT +DDW I Sbjct: 150 DGGYVLNGTKHFCSGAKGSDLLFVFGVVQDDS-PQQGAIIAAAIPTSRAGVTPNDDWAAI 208 Query: 211 GQRGTGSGRVEAVNVFVPDGHVAGGPGAY------SACSSFFAAQFHVNFAAQFVGMAAG 264 G R T SG + NV V V G P A+ S S FA + FA ++G+A G Sbjct: 209 GMRQTDSGSTDFHNVKVEPDEVLGAPNAFVLAFIQSERGSLFAPIAQLIFANVYLGIAHG 268 Query: 265 AFREARQYILEQARPWQGAG--RAAEDPFTLLRMGDMAVRIDSARQLVLRASRLLGACHE 322 A AR+Y QARPW AG +A EDP+T+ G+ + + A A+ LL + Sbjct: 269 ALDAAREYTRTQARPWTPAGIQQATEDPYTIRSYGEFTIALQGADAAAREAAHLLQTVWD 328 Query: 323 Q-----PELLPEAQTAASRAKVAATDAALEVTGSVFQVMGARAASRKYGIDMYYRNARTL 377 + PE E S K AT+AAL ++ VF+V+GAR +YG D ++RN RT Sbjct: 329 KGDALTPEDRGELMVKVSGVKALATNAALNISSGVFEVIGARGTHPRYGFDRFWRNVRTH 388 Query: 378 TLHDPVDRQREAVGRYEL 395 +LHDPV + VG++ L Sbjct: 389 SLHDPVSYKIADVGKHTL 406 >ref|ZP_00034886.1| COG1960: Acyl-CoA dehydrogenases [Burkholderia fungorum] Length = 420 Score = 171 bits (433), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 133/384 (34%), Positives = 194/384 (50%), Gaps = 25/384 (6%) Query: 23 RRLGAAVIGPSAKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGAGYRGDAVLLTAV 82 RRL A P A DRE R P+ ++A +G G++VP + GG G L V Sbjct: 42 RRLAEA-FAPDAALRDRERRLPWAELDAFVASGLWGITVPREFGGAGVSNG----TLAEV 96 Query: 83 LMELASWCSSTSQIFALHNTGVQLVHALGTAEQQQFFFKEAVEGHLF----ASFGSEANK 138 +A+ S QI H ++++ G+ EQQ+FF+ + G F A G + K Sbjct: 97 TATIAAADGSLGQIPQNHFYALEVLRVGGSVEQQRFFYGRVLAGERFGNALAEIGHKDFK 156 Query: 139 DRTALTSVLEHTDGGYLLNGKKIFATGSPGAKWAFWRSVSAEASGSQDERYMMPIVQLSA 198 RT LT G+ ++G+K + TG+ A W V AE G +D Y+ V Sbjct: 157 RRTRLT----REAAGWHIDGRKFYCTGALYAHW-IPTLVVAEEQG-RDVTYLA-FVPRDT 209 Query: 199 DGVTVSDDWDGIGQRGTGSGRVEAVNVFVPDGHVAGGPGAYSACSSF--FAAQFHVNFAA 256 GVTV+DDWDG GQR TGSG V+ V V V ++ ++ A H AA Sbjct: 210 PGVTVTDDWDGFGQRVTGSGSVQFDYVRVEPEWVVPFQASFERPTTIGPLAQIIH---AA 266 Query: 257 QFVGMAAGAFREARQYILEQARPWQGAG--RAAEDPFTLLRMGDMAVRIDSARQLVLRAS 314 +G A GAF +++ E++RPW A RAA+DP TL + GD++VR+ +A L+ RA Sbjct: 267 IDLGQARGAFEAGLKFVRERSRPWIDAKVERAADDPLTLAQFGDISVRLRAAEALLRRAG 326 Query: 315 RLLGACHEQ--PELLPEAQTAASRAKVAATDAALEVTGSVFQVMGARAASRKYGIDMYYR 372 R + A Q + + +A A + A+ T A+L+ +F++ G A G+D ++R Sbjct: 327 RFVDAAQAQLTEQSVAQASIAVAEARALTTTASLDAGSRLFELAGTAATLDGLGLDRFWR 386 Query: 373 NARTLTLHDPVDRQREAVGRYELG 396 NART TLHDPV + AVG + L Sbjct: 387 NARTHTLHDPVRWKYHAVGNFYLN 410 >gb|AAP80184.1| DszC [Rhodococcus erythropolis] Length = 417 Score = 170 bits (431), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 124/378 (32%), Positives = 178/378 (47%), Gaps = 20/378 (5%) Query: 33 SAKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGAGYRGDAVLLTAVLMELASWCSS 92 +A E DR G L +G L VP + GG GA D V+ E+A+ S Sbjct: 34 TAVERDRAGGSATAEREDLHASGLLSLLVPREYGGWGA----DWPTAIEVVREIAAADGS 89 Query: 93 TSQIFALHNTGVQLVHALGTAEQQQFFFKEAVEGHLFASFGSEANKDRTA--LTSVLEHT 150 +F H T ++ +G+ EQ++ + + + + + S N S Sbjct: 90 LGHLFGYHLTNAPMIELIGSQEQEEHLYTQIAQNNWWTGNASSENNSHVLDWKVSATPTE 149 Query: 151 DGGYLLNGKKIFATGSPGAKWAFWRSVSAEASGSQDERYMMPIVQLSADGVTVSDDWDGI 210 DGGY+LNG K F +G+ G+ F V+ + S Q + + S GVT +DDW I Sbjct: 150 DGGYVLNGTKHFCSGAKGSDLLFVFGVAQDDS-PQQGAIIAAAIPTSRAGVTPNDDWAAI 208 Query: 211 GQRGTGSGRVEAVNVFVPDGHVAGGPGAY------SACSSFFAAQFHVNFAAQFVGMAAG 264 G R T SG NV V V G P A+ S S FA + FA ++G+A G Sbjct: 209 GMRQTDSGSTGFHNVKVEPDEVLGAPNAFVLAFIQSERGSLFAPIAQLIFANVYLGIAHG 268 Query: 265 AFREARQYILEQARPWQGAG--RAAEDPFTLLRMGDMAVRIDSARQLVLRASRLLGACHE 322 A AR+Y QARPW AG +A EDP+T+ G+ + + A A+ LL + Sbjct: 269 ALDAAREYTRTQARPWTPAGIQQATEDPYTIRSYGEFTIALQGADAAAREAAHLLQTVWD 328 Query: 323 Q-----PELLPEAQTAASRAKVAATDAALEVTGSVFQVMGARAASRKYGIDMYYRNARTL 377 + PE E S K AT+AAL ++ VF+V+GAR +YG D ++RN RT Sbjct: 329 KGDALTPEDRGELMVKVSGVKALATNAALNISSGVFEVIGARGTYPRYGFDRFWRNVRTH 388 Query: 378 TLHDPVDRQREAVGRYEL 395 +LHDPV + VG++ L Sbjct: 389 SLHDPVSYKIADVGKHTL 406 >gb|AAQ96171.1| dibenzothiophene desulfurization sulfur oxidation enzyme C [Gordonia sp. CYKS2] Length = 416 Score = 170 bits (431), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 124/378 (32%), Positives = 176/378 (46%), Gaps = 20/378 (5%) Query: 33 SAKETDREGRYPYTGINALRVAGFTGLSVPVKDGGLGAGYRGDAVLLTAVLMELASWCSS 92 +A E DR G LR +G L +P + GG GA D V+ E+A+ S Sbjct: 33 TAVERDRTGGSATIEREDLRASGLLSLLIPRQYGGWGA----DWPTAIEVVREIAAADGS 88 Query: 93 TSQIFALHNTGVQLVHALGTAEQQQFFFKEAVEGHLFASFGSEANKDRTA--LTSVLEHT 150 + H + ++ G+ EQ+Q +++ + + S N S Sbjct: 89 LGHLLGYHLSSAPMIELFGSQEQEQRLYRQIAQNDWWTGNASSENNSHVLDWKVSASPTE 148 Query: 151 DGGYLLNGKKIFATGSPGAKWAFWRSVSAEASGSQDERYMMPIVQLSADGVTVSDDWDGI 210 DGGYLLNG K F +G+ G+ V + S Q + ++ S GV V+DDW I Sbjct: 149 DGGYLLNGTKHFCSGAKGSDLLLVFGVIQDDSPQQGA-IIAAVIPTSRHGVQVNDDWAAI 207 Query: 211 GQRGTGSGRVEAVNVFVPDGHVAGGPGAY------SACSSFFAAQFHVNFAAQFVGMAAG 264 G R T SG + +V V V G P A+ S S FA + FA ++G+A G Sbjct: 208 GMRQTDSGSTDFHSVKVEPDEVLGEPNAFIVAFIQSERGSLFAPIVQLIFANVYLGIAHG 267 Query: 265 AFREARQYILEQARPWQGAG--RAAEDPFTLLRMGDMAVRIDSARQLVLRASRLLGACHE 322 A AR+Y QARPW AG +A EDP+ L G+ + + A A+ LL + Sbjct: 268 ALDAAREYTRTQARPWTPAGVQQATEDPYVLRAYGEFTIALQGADAAAREAAHLLQTVWD 327 Query: 323 Q-----PELLPEAQTAASRAKVAATDAALEVTGSVFQVMGARAASRKYGIDMYYRNARTL 377 + PE E S K AT+AAL+V +F+V+GAR KYG D ++RN RT Sbjct: 328 KGDALTPEDRGELMVKISGVKALATNAALDVNSGIFEVIGARGTHPKYGFDRFWRNVRTH 387 Query: 378 TLHDPVDRQREAVGRYEL 395 TLHDPV + VG++ L Sbjct: 388 TLHDPVSYKIADVGKHTL 405 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.133 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 554,371,760 Number of extensions: 22327661 Number of successful extensions: 53468 Number of sequences better than 1.0e-05: 793 Number of HSP's gapped: 52635 Number of HSP's successfully gapped: 793 Length of query: 397 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 269 Effective length of database: 409,704,906 Effective search space: 110210619714 Effective search space used: 110210619714 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)