BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0056.p (310 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00090386.1| COG2141: Coenzyme F420-dependent N5,N10-methy... 309 5e-83 ref|ZP_00125017.1| COG2141: Coenzyme F420-dependent N5,N10-methy... 297 2e-79 ref|NP_744890.1| conserved hypothetical protein [Pseudomonas put... 296 5e-79 ref|NP_792781.1| xenobiotic compound monooxygenase, DszA family ... 295 7e-79 ref|ZP_00127066.1| COG2141: Coenzyme F420-dependent N5,N10-methy... 295 1e-78 >ref|ZP_00090386.1| COG2141: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases [Azotobacter vinelandii] Length = 467 Score = 309 bits (792), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 154/306 (50%), Positives = 212/306 (69%), Gaps = 3/306 (0%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 HDERYD ADEY+E+ YKL EGSWED AV DRE R+FTDP+KVH+I H G++F+++G H Sbjct: 154 HDERYDHADEYLEVIYKLLEGSWEDGAVIRDRERRLFTDPAKVHEIDHRGEHFQVSGIHL 213 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 EPS QRTPV+FQAG+S RGR F+A+HAE VF+ AP+ VL++ + DIR+RA E GRDP Sbjct: 214 CEPSPQRTPVLFQAGASSRGRRFSAEHAECVFVAAPSKAVLKKTVADIRQRAVEAGRDPY 273 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFENIH 184 +++ +V++G T EA+ K +Y Y S E L+ + TG+D + PD ++IH Sbjct: 274 QIRILNLQTVILGETDAEAQAKWRDYKQYVSYEGALALISGWTGLDFGQYQPDQVLKHIH 333 Query: 185 TEMGQTHTDRFTKHSKTKK-TVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDVD 243 T Q+ + F+ +K+ TVRE+ D++ G G +G+P+ +ADQ+Q WVEETDVD Sbjct: 334 TNAIQSAVEAFSTADPSKQWTVRELA-DWVGIGGFGPLFVGSPQTVADQLQEWVEETDVD 392 Query: 244 GFNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTVF 303 GFNL V ++ D V+L+VP LQ+RG+FK +Y GT REKLFG G RLP++HPG + Sbjct: 393 GFNLAYAVSHETFVDVVELLVPELQKRGVFKTDYAPGTLREKLFGAG-PRLPDNHPGARY 451 Query: 304 RNPATL 309 R+ A L Sbjct: 452 RDLAAL 457 >ref|ZP_00125017.1| COG2141: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases [Pseudomonas syringae pv. syringae B728a] Length = 452 Score = 297 bits (760), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 144/301 (47%), Positives = 200/301 (66%), Gaps = 4/301 (1%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 HD RYD ADEY+E+ YKL+EGSWEDDAV DRE RV+ P KVHK++H+G+++++ GYH Sbjct: 155 HDRRYDQADEYLEVLYKLWEGSWEDDAVINDREQRVYAQPGKVHKVRHQGEFYQVEGYHL 214 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 EPS QRTPV+FQAGSS+RG FA ++AE VF+ REQ+ +R A + GR+P Sbjct: 215 CEPSPQRTPVLFQAGSSERGLQFAGQNAECVFISGQNKAATREQVDKVRASAVQAGRNPE 274 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFENIH 184 +KVF GL+V+V T+ A KH EY + SAEA ++ +A TGID ++ + D + + Sbjct: 275 DIKVFMGLNVIVAATEALAREKHAEYRRHASAEAGVAHFAASTGIDFSQYELDEPIQYVK 334 Query: 185 TEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDVDG 244 Q+ TK+ K R+ D G R +T+IG+P ++AD+++ W+EET +DG Sbjct: 335 NNAIQSA----TKNLKNNDWTRQKLLDQHALGGRYITLIGSPRQVADELESWIEETGLDG 390 Query: 245 FNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTVFR 304 FNL V P SYEDF+DLV+P LQ+RG +K Y+EG+ R+KLF +G RLPE H G R Sbjct: 391 FNLTRIVTPESYEDFIDLVIPELQRRGSYKTAYQEGSLRKKLFPQGTDRLPERHAGAAHR 450 Query: 305 N 305 + Sbjct: 451 H 451 >ref|NP_744890.1| conserved hypothetical protein [Pseudomonas putida KT2440] gb|AAN68354.1| conserved hypothetical protein [Pseudomonas putida KT2440] Length = 469 Score = 296 bits (757), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 145/301 (48%), Positives = 201/301 (66%), Gaps = 1/301 (0%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 HD RYD ADEY+E+ YKLFEGSWE+ AV DRE +F+DP+KVH+I+H+GK+F++ G H Sbjct: 154 HDARYDYADEYLEVLYKLFEGSWEEGAVLRDRERGIFSDPNKVHEIRHQGKHFQVPGIHL 213 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 EPS QRTPV++QAG+S RG+ FAA HAE VF+ AP+ +L++ + DIRRRA E GRDP Sbjct: 214 CEPSPQRTPVLYQAGASSRGKDFAAGHAECVFVAAPSKVILKKTVADIRRRAAEAGRDPR 273 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFENIH 184 V +F +V+V T +A+ K E SY S E L+ + TGID + PD ++IH Sbjct: 274 KVLIFNLQTVIVDETDAKAQAKWQELASYSSYEGALALISGWTGIDFGQYRPDQVLKHIH 333 Query: 185 TEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDVDG 244 T Q+ + F+ K+ + D++ G G ++G+ + +AD++Q WVEETDVDG Sbjct: 334 TNAIQSAVETFSTADPDKQWTVQELADWVGIGGFGPLIVGSAQTVADELQAWVEETDVDG 393 Query: 245 FNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTVFR 304 FNL + ++ D VDL+ P LQ+RG++K EY GT REKLFG G RLP +HPG +R Sbjct: 394 FNLAYVLAHETFRDVVDLLEPELQKRGVYKTEYRSGTLREKLFGDG-PRLPANHPGAGYR 452 Query: 305 N 305 + Sbjct: 453 D 453 >ref|NP_792781.1| xenobiotic compound monooxygenase, DszA family [Pseudomonas syringae pv. tomato str. DC3000] gb|AAO56476.1| xenobiotic compound monooxygenase, DszA family [Pseudomonas syringae pv. tomato str. DC3000] Length = 466 Score = 295 bits (756), Expect = 7e-79, Method: Compositional matrix adjust. Identities = 144/306 (47%), Positives = 202/306 (66%), Gaps = 1/306 (0%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 HD RYD A+EY+E+ YKL+EGSWED A+ DRE RVF+DPSK+H+I H GK+F++ G H Sbjct: 154 HDARYDYAEEYLEVCYKLWEGSWEDGAILRDRERRVFSDPSKIHEIGHVGKHFQVPGIHL 213 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 EPS QRTPV++QAG+S RG+ FAA+HAE VF+ AP+ +L++ + DIRRR E GRDP+ Sbjct: 214 CEPSPQRTPVLYQAGASSRGKQFAAEHAECVFVAAPSKVLLKKTVADIRRRTAEAGRDPS 273 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFENIH 184 V + +V++G T +A+ K EY S+ S E ++ + TGID ++ PD ++H Sbjct: 274 KVLILNLQTVILGETDAKAKAKFEEYKSWVSYEGAMALISGWTGIDFSQFKPDEPLRHVH 333 Query: 185 TEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDVDG 244 T Q+ + F+ + D++ G G +G+PE +AD +Q WVE+TDVDG Sbjct: 334 TNAIQSAVETFSTADPNTVWTPQALADWVGIGGFGPLFVGSPETVADLLQEWVEDTDVDG 393 Query: 245 FNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTVFR 304 FNL + ++ D VDL+VP LQ+RG++K EY +GT REKLFG G RL HPG FR Sbjct: 394 FNLAYALTHETFIDAVDLLVPELQKRGVYKTEYAQGTLREKLFGDG-PRLETGHPGAAFR 452 Query: 305 NPATLS 310 + A L+ Sbjct: 453 DLAALN 458 >ref|ZP_00127066.1| COG2141: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases [Pseudomonas syringae pv. syringae B728a] Length = 466 Score = 295 bits (754), Expect = 1e-78, Method: Compositional matrix adjust. Identities = 145/305 (47%), Positives = 202/305 (66%), Gaps = 1/305 (0%) Query: 5 HDERYDSADEYMELSYKLFEGSWEDDAVKIDRENRVFTDPSKVHKIKHEGKYFKLTGYHS 64 HD RYD A+EY+E+ YKL+EGSWED A+ DRE RVF+DPSK+H+I+H GK+F++ G H Sbjct: 154 HDARYDYAEEYLEVCYKLWEGSWEDGAILRDRERRVFSDPSKIHEIRHVGKHFQVPGIHL 213 Query: 65 SEPSLQRTPVIFQAGSSDRGRAFAAKHAEGVFLKAPTTEVLREQIKDIRRRAREYGRDPA 124 EPS QRTPV++QAG+S RG+ FAA+HAE VF+ AP+ +L++ + DIRRR E GRDP+ Sbjct: 214 CEPSPQRTPVLYQAGASSRGKQFAAEHAECVFVAAPSKVLLKKTVADIRRRTAEAGRDPS 273 Query: 125 AVKVFTGLSVVVGRTKEEAERKHTEYLSYQSAEATLSSYANVTGIDLTKLDPDSYFENIH 184 V +F +V++G T +A K EY S+ S E ++ + TGID ++ PD ++H Sbjct: 274 KVLIFNLQTVILGETDVKARAKFEEYKSWVSYEGAMALISGWTGIDFSQFKPDEPLRHVH 333 Query: 185 TEMGQTHTDRFTKHSKTKKTVREVTEDFLKKGFRGLTVIGTPEEIADQIQYWVEETDVDG 244 T Q+ + F+ + D++ G G +G+PE +AD +Q WVEETDVDG Sbjct: 334 TNAIQSAVETFSTADPNTIWTPQALADWVGIGGFGPLFVGSPETVADLLQEWVEETDVDG 393 Query: 245 FNLEPYVLPASYEDFVDLVVPVLQQRGIFKKEYEEGTFREKLFGKGQARLPEHHPGTVFR 304 FNL + ++ D V+L+VP LQ+RG++K EY +GT REKLFG G RL HPG FR Sbjct: 394 FNLAYALTHETFIDAVELLVPELQKRGVYKTEYAKGTLREKLFGDG-PRLEAGHPGAGFR 452 Query: 305 NPATL 309 + A L Sbjct: 453 DLAAL 457 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 480,220,253 Number of extensions: 20420169 Number of successful extensions: 53674 Number of sequences better than 1.0e-05: 221 Number of HSP's gapped: 53446 Number of HSP's successfully gapped: 225 Length of query: 310 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 185 Effective length of database: 417,850,203 Effective search space: 77302287555 Effective search space used: 77302287555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)