BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0036.p (249 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00188348.1| hypothetical protein Rxyl041301 [Rubrobacter ... 243 2e-63 ref|NP_107683.1| hypothetical protein mll7342 [Mesorhizobium lot... 213 3e-54 ref|NP_103660.1| hypothetical protein mll2277 [Mesorhizobium lot... 201 2e-50 gb|EAJ42133.1| unknown [environmental sequence] 176 4e-43 ref|NP_898784.1| conserved hypothetical protein [Rhodococcus ery... 167 1e-40 >ref|ZP_00188348.1| hypothetical protein Rxyl041301 [Rubrobacter xylanophilus DSM 9941] Length = 274 Score = 243 bits (621), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 116/221 (52%), Positives = 155/221 (70%), Gaps = 1/221 (0%) Query: 7 GGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDITSGGR 66 GG+ +ADS+ + ++ ETG LPVYYFP +DV L + ++ P KG +W + +GGR Sbjct: 43 GGETVADSRRMKLLHETGLLPVYYFPEEDVRTELLERTDHTTRCPFKGEAVYWTVRAGGR 102 Query: 67 TARNAVWTYSGPQPEAHFLHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIPASRHV 126 TA NA W Y P A L G+++F W +MD WFEE+EEV VH RDPY R+DA+P+SRHV Sbjct: 103 TAENAAWAYPEPLEGAPPLGGHIAFYWDRMDRWFEEDEEVDVHPRDPYHRIDALPSSRHV 162 Query: 127 QLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIATYWTAA 186 ++ VNG +VA++RRPVIL+ETGL PRYY+P EDVR +LL + +S+ CPYKG+A+YW+ Sbjct: 163 RVTVNGELVAETRRPVILFETGLPPRYYIPREDVREELLVPSESSSVCPYKGVASYWSVR 222 Query: 187 VNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDG 227 G EDLVWSY EP + + GLLCF++E D L VDG Sbjct: 223 AGGETVEDLVWSYPEPRRDAERVGGLLCFFNERVD-LEVDG 262 Score = 99.0 bits (245), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 47/103 (45%), Positives = 64/103 (62%) Query: 122 ASRHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIAT 181 + R V++E+ G VADSRR +L+ETGL P YY P EDVR +LL+ ++TRCP+KG A Sbjct: 34 SPRRVRVELGGETVADSRRMKLLHETGLLPVYYFPEEDVRTELLERTDHTTRCPFKGEAV 93 Query: 182 YWTAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALY 224 YWT GR E+ W+Y EPL + G + FY + D + Sbjct: 94 YWTVRAGGRTAENAAWAYPEPLEGAPPLGGHIAFYWDRMDRWF 136 >ref|NP_107683.1| hypothetical protein mll7342 [Mesorhizobium loti MAFF303099] dbj|BAB53469.1| hypothetical protein [Mesorhizobium loti] Length = 247 Score = 213 bits (543), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 102/230 (44%), Positives = 140/230 (60%) Query: 2 LXXXXGGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDI 61 L G +AD+ + +V+ E+ LPVYYFP+ DV PS P KG+ T + + Sbjct: 7 LRVQVGSMIVADTTKALVLYESDHLPVYYFPMSDVREEFLLPSRTTTESPFKGVATHYSL 66 Query: 62 TSGGRTARNAVWTYSGPQPEAHFLHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIP 121 +G +A W Y P + L GY+SF W KM W+EE+EE+FVHARDP+ RVD +P Sbjct: 67 NTGITLVEDAAWRYLDPVKGSPDLSGYMSFYWPKMTHWYEEDEEIFVHARDPFRRVDCLP 126 Query: 122 ASRHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIAT 181 +SR VQ+ ++G VADSRR V L+ETG R+YLP D RLD+ + +RCPYKGI+ Sbjct: 127 SSRRVQVILDGEQVADSRRGVFLFETGHPVRHYLPISDTRLDMFAPSRYISRCPYKGISN 186 Query: 182 YWTAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDGIKWS 231 Y+ + +E+LVW Y EP+ E I GL+CF+HE D + VDG+ S Sbjct: 187 YYHVTTPQKRHENLVWYYPEPVHEAERIKGLVCFHHELVDKILVDGVDIS 236 Score = 71.2 bits (173), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 31/97 (31%), Positives = 57/97 (58%) Query: 120 IPASRHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGI 179 +P + ++++V +IVAD+ + ++LYE+ P YY P DVR + L + +T P+KG+ Sbjct: 1 MPMQKRLRVQVGSMIVADTTKALVLYESDHLPVYYFPMSDVREEFLLPSRTTTESPFKGV 60 Query: 180 ATYWTAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFY 216 AT+++ ED W Y +P+ +++G + FY Sbjct: 61 ATHYSLNTGITLVEDAAWRYLDPVKGSPDLSGYMSFY 97 >ref|NP_103660.1| hypothetical protein mll2277 [Mesorhizobium loti MAFF303099] dbj|BAB49446.1| hypothetical protein [Mesorhizobium loti] Length = 257 Score = 201 bits (510), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 106/224 (47%), Positives = 153/224 (68%), Gaps = 4/224 (1%) Query: 7 GGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDITSGGR 66 GG IADS+ V+V+ LP+Y+FP V+A++ +PS H G +WDI SG R Sbjct: 32 GGRTIADSRNVLVLRSNHFLPIYFFPPSSVDASVLKPSLHREPHAVGGETAYWDIDSGDR 91 Query: 67 TARNAVWTYSGPQPEAHF--LHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIPASR 124 A NA W+++ P P+ + L G ++F+W +D WFEEEEEVFVHARDPY R+D + +S Sbjct: 92 RAANAAWSFTAP-PDENLSPLAGRIAFTWKAVDQWFEEEEEVFVHARDPYARIDVLHSSS 150 Query: 125 HVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIATYWT 184 HVQ+ +G I+ADS RPV+L+ET L R+YL EDVRL+ L + ++TRCPYKGIA+YW+ Sbjct: 151 HVQVWFDGDIIADSHRPVLLFETHLPTRFYLRPEDVRLERLTASSSTTRCPYKGIASYWS 210 Query: 185 AAV-NGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDG 227 + +G D+ WSY++P+ E+ +I GL+ FY +A D +++DG Sbjct: 211 GLLKDGSVRPDIAWSYRDPIDEMPKIKGLIAFYPQAVDRIHLDG 254 >gb|EAJ42133.1| unknown [environmental sequence] Length = 250 Score = 176 bits (447), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 78/220 (35%), Positives = 127/220 (57%), Gaps = 1/220 (0%) Query: 7 GGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDITSGGR 66 + I DSK+ ++ E G LP+YYFP + + L + K P KG T+WD Sbjct: 32 NNEMIIDSKKTFILLEKGHLPIYYFPKESIRMDLLIKNTKTTFCPLKGEATYWDFNYKNE 91 Query: 67 TARNAVWTYSGPQPEAHFLHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIPASRHV 126 + W Y P + L G V+F W+K+D W+EE EE++ + RDPY RVDA+ +SR+V Sbjct: 92 NILDIAWCYENPIQQQRELRGLVAFYWNKLDNWYEEAEEIYSYPRDPYVRVDALKSSRNV 151 Query: 127 QLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIATYWTAA 186 ++ ++ ++ + R VIL+ET P+Y+ P D++++L+ + RCPYKGI+ Y + Sbjct: 152 KIFLDDNLILTTNRSVILFETHKKPKYFFPINDIKVNLVF-SEKIFRCPYKGISNYLSIK 210 Query: 187 VNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVD 226 N + ++D+V Y EP PEV + L+CF + ++ +D Sbjct: 211 DNNKTFKDIVMQYTEPRPEVSILKNLVCFENNNLLSIIID 250 Score = 82.4 bits (202), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 38/103 (36%), Positives = 56/103 (54%) Query: 122 ASRHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIAT 181 + + V+ +N ++ DS++ IL E G P YY P E +R+DLL +N +T CP KG AT Sbjct: 23 SEKWVRGRINNEMIIDSKKTFILLEKGHLPIYYFPKESIRMDLLIKNTKTTFCPLKGEAT 82 Query: 182 YWTAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALY 224 YW D+ W Y+ P+ + E+ GL+ FY D Y Sbjct: 83 YWDFNYKNENILDIAWCYENPIQQQRELRGLVAFYWNKLDNWY 125 >ref|NP_898784.1| conserved hypothetical protein [Rhodococcus erythropolis] gb|AAP74054.1| conserved hypothetical protein [Rhodococcus erythropolis] Length = 265 Score = 167 bits (424), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 88/223 (39%), Positives = 123/223 (55%), Gaps = 3/223 (1%) Query: 7 GGDYIADSKEVMVVTETGRLPVYYFPLKDVNAALFQPSEKLATHPQKGIQTFWDITSGGR 66 G IAD+ + + E G LPVYY P DV +P + P KG +WD+ R Sbjct: 34 AGHVIADTTRAVYLFEKGHLPVYYIPRDDVRTDSLEPLDATTWCPWKGKARYWDLVVDDR 93 Query: 67 TARNAVWTYSGPQPEAHFLHGYVSFSWSKMDGWFEEEEEVFVHARDPYTRVDAIPASRHV 126 A ++W Y P ++ L YV+F W KMD W+EE+++VFVH RDPY RVD +P+SRHV Sbjct: 94 RAERSLWGYDEPLKDSLDLSWYVAFYWHKMDAWYEEDQQVFVHPRDPYVRVDVLPSSRHV 153 Query: 127 QLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIATY--WT 184 ++ + +VAD+ P +L+ET L RYY+P DVR + + T CPYKG A+Y +T Sbjct: 154 EVFIGDTLVADTTHPRLLFETSLPVRYYIPRIDVRSEFFTGSDAKTSCPYKGTASYLSFT 213 Query: 185 AAVNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALYVDG 227 + + ED W Y E I + FY + A + VDG Sbjct: 214 GSDDSDPVEDAAWYYPFTTAEASGIGDHVSFYPDGA-RIVVDG 255 Score = 78.6 bits (192), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 38/101 (37%), Positives = 58/101 (57%) Query: 124 RHVQLEVNGIIVADSRRPVILYETGLTPRYYLPAEDVRLDLLQENPNSTRCPYKGIATYW 183 + +++ G ++AD+ R V L+E G P YY+P +DVR D L+ +T CP+KG A YW Sbjct: 27 KRIRVFFAGHVIADTTRAVYLFEKGHLPVYYIPRDDVRTDSLEPLDATTWCPWKGKARYW 86 Query: 184 TAAVNGREYEDLVWSYKEPLPEVHEIAGLLCFYHEAADALY 224 V+ R E +W Y EPL + +++ + FY DA Y Sbjct: 87 DLVVDDRRAERSLWGYDEPLKDSLDLSWYVAFYWHKMDAWY 127 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 403,226,562 Number of extensions: 17036856 Number of successful extensions: 30509 Number of sequences better than 1.0e-05: 65 Number of HSP's gapped: 30482 Number of HSP's successfully gapped: 91 Length of query: 249 Length of database: 757,237,578 Length adjustment: 123 Effective length of query: 126 Effective length of database: 423,280,401 Effective search space: 53333330526 Effective search space used: 53333330526 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 124 (52.4 bits)