BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0035.p (531 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_435411.1| putative histidine ammonia-lyase [Sinorhizobium... 348 2e-94 ref|NP_107503.1| histidine ammonia-lyase [Mesorhizobium loti MAF... 301 2e-80 ref|NP_622497.1| Histidine ammonia-lyase [Thermoanaerobacter ten... 293 6e-78 ref|NP_103248.1| histidine ammonia-lyase [Mesorhizobium loti MAF... 293 6e-78 ref|NP_718622.1| Pal/histidase family protein [Shewanella oneide... 284 3e-75 >ref|NP_435411.1| putative histidine ammonia-lyase [Sinorhizobium meliloti 1021] pir||E95282 probable histidine ammonia-lyase (EC 4.3.1.3) [imported] - Sinorhizobium meliloti (strain 1021) magaplasmid pSymA gb|AAK64823.1| putative histidine ammonia-lyase [Sinorhizobium meliloti] Length = 546 Score = 348 bits (892), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 212/524 (40%), Positives = 290/524 (55%), Gaps = 32/524 (6%) Query: 26 LVLDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGVGWN 85 ++L+G N T + VA+G AV +D + ++E A + A +G +YGL GVGWN Sbjct: 25 VLLNGRNATPEMIVRVAKGE-AVTVDGESLDKVERAYAVLLQGAKEGQEIYGLTVGVGWN 83 Query: 86 KDL-------EISPDFFEA---YNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGAA 135 KD E++P+ EA +N LL +HS GV P+A VVRA M RLN +LTG Sbjct: 84 KDRKMVDATGELTPELMEASREFNEGLLRAHSVGVGPDAAVPVVRAAMAVRLNNILTGGP 143 Query: 136 GVQPELAGRYADFLNLGIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVPALE 195 GVQP +A FLN GI P +P RGSVG AD+T +SHIGLAM+GEG+ G R+ A E Sbjct: 144 GVQPHVAEMLLAFLNKGITPTMPSRGSVGQADMTLLSHIGLAMLGEGDVDYQGRRMSAAE 203 Query: 196 ALKAAGLAPLRLGPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGNV 255 ALKAAG+ PL+ KDGLA++ +D E+LL T+ +YALSLE + GNV Sbjct: 204 ALKAAGIEPLKPFGKDGLAILSSNAYAAGLAALAIHDAEQLLSTSRLVYALSLEGLNGNV 263 Query: 256 SPLDEAVHRKRPFPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARDA 315 SPL E V RPFP L S +R+ L GS LW + LQDPLSFR A + G+ D+ Sbjct: 264 SPLLEDVAALRPFPSHLSSTTELRALLEGSYLWQADDKRILQDPLSFRTAPYLLGSFADS 323 Query: 316 HDFTSRQLVLHLNSSDDNPCV---------LSDEQR-------IVSCANYDPLSWTLGFE 359 T + + +NSSDDNP + L +R ++ AN++PL W + FE Sbjct: 324 LARTKALVQIQINSSDDNPGIVVGVEPKSDLFQARRGYVEGGAVLPTANFEPLPWIIAFE 383 Query: 360 MLGSALHHVSKSSCHRIIKLGDPRFTGLTRFLTADTSRSVGFCTVQKTATSLDAEIRHLS 419 LG L H + +S R++KL +P T L R+L + + F V+ +L E R L+ Sbjct: 384 ELGIVLAHHTTASSERVLKLNNPTHTKLARYLGTENTHH-AFLVVEAPLMALATENRALA 442 Query: 420 NPVSADYLSLAGEIEDHAANSPYVVSKTREILDRLTYILAIELLHAAQAVDLR----GGL 475 P S D +AG +ED N+P VV + R +D IL++E+LHAAQA DLR Sbjct: 443 QPTSFDSRPIAGGVEDVGTNAPLVVERIRAQIDNSFTILSMEMLHAAQAADLRLKGHPER 502 Query: 476 TLGHASWAGYKSLRRAVPFLSEDRNQSEDIERAYRLLKDGTWLN 519 L A+ + + + R+ VPFL DR+ + DI LK+ N Sbjct: 503 KLSTATTSFHDAFRQRVPFLDRDRSMTPDIAAGTAFLKEYALTN 546 >ref|NP_107503.1| histidine ammonia-lyase [Mesorhizobium loti MAFF303099] dbj|BAB53289.1| histidine ammonia-lyase [Mesorhizobium loti] Length = 498 Score = 301 bits (772), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 174/488 (35%), Positives = 275/488 (56%), Gaps = 1/488 (0%) Query: 26 LVLDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGVGWN 85 ++LD L +ADV +AR +++ + A +R+ A+R I + G P+YG+ G+G Sbjct: 6 VLLDAKPLGVADVAEIARRNARLMLGEEAMRRIRASRALIEHLTGLGKPMYGVTTGLGAC 65 Query: 86 KDLEISPDFFEAYNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGAAGVQPELAGRY 145 D ++ A+ ++ LSHS G+ P T+ VRA+M AR++G+ G G + Sbjct: 66 VDTPLAQADLIAFQHSVPLSHSMGIGPALPTEAVRALMTARISGMAAGGTGTSERVVMGL 125 Query: 146 ADFLNLGIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVPALEALKAAGLAPL 205 LN G+HPV+P GS+GAAD+ + H+ ++ G+GEA G +P+ EAL A L PL Sbjct: 126 VAALNAGVHPVIPTWGSIGAADLAHLGHMARSLRGDGEAEFQGRIMPSAEALALARLEPL 185 Query: 206 RLGPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGNVSPLDEAVHRK 265 L KDG A++ D+ L++ A AL+ EA R +++ +DE Sbjct: 186 DLREKDGHAIIVANSLSTGTACLYLEDVAHLIDWALAAVALNYEAFRSSLTAIDEQALAA 245 Query: 266 RPFPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARDAHDFTSRQLVL 325 RP GQ + +R+ LAGS LW D LQDPLS+R Q+ G +A + + Sbjct: 246 RPAFGQREIGARLRAELAGSGLWQDKAARRLQDPLSYRCVPQVWGGLLNAFEQARLATEI 305 Query: 326 HLNSSDDNPCVLSDEQRIVSCANYDPLSWTLGFEMLGSALHHVSKSSCHRIIKLGDPRFT 385 L S DNP +L++ +R++S N+D + TL +E LG AL H + + +R +KL P Sbjct: 306 ELTHSGDNPVILAEGERVISNGNFDLTAMTLAWEQLGQALAHCAVGTANRCMKLMSPTTA 365 Query: 386 GLTRFLTADTSRSVGFCTVQKTATSLDAEIRHLSNPVSADYLSLAGEIEDHAANSPYVVS 445 L RFL+A G+ +QK ++L+AEIRHL+NP+S L+++ IED ++ +P VV+ Sbjct: 366 ELPRFLSARGGSRQGYAELQKPLSALEAEIRHLANPMSLSPLAVSDGIEDQSSMAPRVVA 425 Query: 446 KTREILDRLTYILAIELLHAAQAVDLRGGL-TLGHASWAGYKSLRRAVPFLSEDRNQSED 504 KT EI++R+ Y++A+EL+ AA V+LRG + T+G + ++R V L +DR S D Sbjct: 426 KTAEIVERMRYLVAMELIFAATGVELRGVVDTMGEGPQRTFAAVRALVSPLDDDREMSTD 485 Query: 505 IERAYRLL 512 + R R++ Sbjct: 486 MTRVARMV 493 >ref|NP_622497.1| Histidine ammonia-lyase [Thermoanaerobacter tengcongensis] sp|Q8RBH4|HUTH_THETN Histidine ammonia-lyase (Histidase) gb|AAM24101.1| Histidine ammonia-lyase [Thermoanaerobacter tengcongensis] Length = 508 Score = 293 bits (751), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 173/494 (35%), Positives = 264/494 (53%), Gaps = 1/494 (0%) Query: 23 LNPLVLDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGV 82 + +V+DGNNLTI DV VAR + VV+ + A +R+ +R + +G VYG+ G Sbjct: 1 MKKVVIDGNNLTIEDVVKVARDKWEVVLSEVAKERIVHSREIVDRYVKEGKVVYGITTGF 60 Query: 83 GWNKDLEISPDFFEAYNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGAAGVQPELA 142 G D+ IS + EA +NL++SHS V +VVRA+ML R N L G +GV+ E Sbjct: 61 GKFSDVVISKEDTEALQKNLIMSHSCAVGDPLPEEVVRAIMLLRANALAKGFSGVRLETV 120 Query: 143 GRYADFLNLGIHPVLPLRGSVGAA-DITSISHIGLAMIGEGEAHMDGSRVPALEALKAAG 201 + +N + PV+P +GS+GA+ D+ ++H+ L MIG GEA G RV EA++ AG Sbjct: 121 ETLIEMINKNVVPVIPEKGSLGASGDLAPLAHMVLVMIGRGEAFYAGERVSGEEAMRRAG 180 Query: 202 LAPLRLGPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGNVSPLDEA 261 + + L K+GLA++ YD ++++ AD + +++LEA+RG + D+ Sbjct: 181 IPTITLSSKEGLALINGTQVMSALGCLNVYDAKRIIAVADAVASITLEALRGIIDAFDDR 240 Query: 262 VHRKRPFPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARDAHDFTSR 321 V RP GQ+ S VR + GS+L T +QD + R Q+HGA RDA + R Sbjct: 241 VQMVRPHKGQIVSAKNVRKMVEGSELITRQGEIRVQDAYTLRCIPQVHGAVRDAISYIER 300 Query: 322 QLVLHLNSSDDNPCVLSDEQRIVSCANYDPLSWTLGFEMLGSALHHVSKSSCHRIIKLGD 381 L + +NS+ DNP + D+ ++S N+ L + L AL ++ S RI +L + Sbjct: 301 VLGVEINSATDNPLIFPDDGEVISGGNFHGEPVALAMDFLSIALSEIANISERRIERLVN 360 Query: 382 PRFTGLTRFLTADTSRSVGFCTVQKTATSLDAEIRHLSNPVSADYLSLAGEIEDHAANSP 441 + L FLT + G Q TA SL +E + LS+P S D + + EDH + Sbjct: 361 YQLNDLPPFLTEKGGLNSGMMIAQYTAASLVSENKVLSHPASVDSIPSSANQEDHVSMGT 420 Query: 442 YVVSKTREILDRLTYILAIELLHAAQAVDLRGGLTLGHASWAGYKSLRRAVPFLSEDRNQ 501 K RE+L +T +LAIELL A+QA++ R G G + Y+ +R V L EDR Sbjct: 421 IAARKAREVLKNVTTVLAIELLTASQALEFRKGFKRGKGTDRIYRLVREKVNPLVEDREL 480 Query: 502 SEDIERAYRLLKDG 515 DI + +++ G Sbjct: 481 YIDINACFDIIRSG 494 >ref|NP_103248.1| histidine ammonia-lyase [Mesorhizobium loti MAFF303099] dbj|BAB49034.1| histidine ammonia-lyase [Mesorhizobium loti] Length = 502 Score = 293 bits (750), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 181/495 (36%), Positives = 264/495 (53%), Gaps = 4/495 (0%) Query: 23 LNPLVLDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGV 82 ++ L+L G + + DV +VAR R V I R++ AR+ + A G +YGLN G+ Sbjct: 1 MSALILTGAGVGVGDVATVARQGRKVEIGPEIIGRLDKARKVLDQAAASGQQIYGLNTGL 60 Query: 83 GWNKDLEISPDFFEAYNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLTGAAGVQPELA 142 G N + D A+ R LL S V + VRA M+AR + L G +G+ P + Sbjct: 61 GANLGTTVDGDA-SAFQRQLLEGRSGAVGETLPIETVRATMVARASMLSAGGSGLSPTVF 119 Query: 143 GRYADFLNLGIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVPALEALKAAGL 202 D LN G+HPV+P GS+GA D+ ++ + +IGEGEA G R+PA +AL A L Sbjct: 120 VALVDALNAGVHPVMPSLGSIGAGDLVLMTALARLLIGEGEADYQGRRMPAAKALMMARL 179 Query: 203 APLRLGPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIRGNVSPLDEAV 262 AP+ L PKDGL+++ D L + AL++E N + LD + Sbjct: 180 APISLAPKDGLSLINASAVSAGSGALAITDALAALAQQQQAGALTMEGFGANRTILDPRL 239 Query: 263 HRKRPFPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAARDAHDFTSRQ 322 RP GQ ++ + LA ++ P ++QDPLS R IHGA +A R Sbjct: 240 QMARPAAGQQEAAKALHDLLARDEMPA---PTTIQDPLSIRCMPSIHGALIEAIGQAKRA 296 Query: 323 LVLHLNSSDDNPCVLSDEQRIVSCANYDPLSWTLGFEMLGSALHHVSKSSCHRIIKLGDP 382 + + LN++ DNP V+ D++ ++S N+ S +L FE LG A+ + +S R I+L Sbjct: 297 VEIELNAAADNPLVIGDDELVLSTGNFHTASLSLAFETLGLAIAQCAAASVARFIQLTGS 356 Query: 383 RFTGLTRFLTADTSRSVGFCTVQKTATSLDAEIRHLSNPVSADYLSLAGEIEDHAANSPY 442 GLT++L+ S GF +QKTATS+ A IRH +NPV D+L ++ +EDHA SP Sbjct: 357 GRNGLTKYLSPIGGASAGFVPLQKTATSILAAIRHKANPVMLDFLPVSEGVEDHATQSPL 416 Query: 443 VVSKTREILDRLTYILAIELLHAAQAVDLRGGLTLGHASWAGYKSLRRAVPFLSEDRNQS 502 VSK ++ ++A EL+ AAQAVDLR GLTL + A Y ++R V L +DR Sbjct: 417 AVSKCAGMIALWRRLIAFELMAAAQAVDLREGLTLAPRTGAIYAAVRAHVSMLKDDRPLG 476 Query: 503 EDIERAYRLLKDGTW 517 D E + +L DG W Sbjct: 477 NDAEVLHAMLADGAW 491 >ref|NP_718622.1| Pal/histidase family protein [Shewanella oneidensis MR-1] gb|AAN56066.1| Pal/histidase family protein [Shewanella oneidensis MR-1] Length = 524 Score = 284 bits (727), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 182/516 (35%), Positives = 256/516 (49%), Gaps = 33/516 (6%) Query: 23 LNPLVLDGNNLTIADVDSVARGRRAVVIDDGAWQRMEAARRTIFAVADQGLPVYGLNRGV 82 + +VLDG +LT ++A G + V I A ++ A + A G PVYGL GV Sbjct: 1 MEQVVLDGKHLTQDQAWAIADGAK-VKIAPQAKTQLVKAHELLMEAARLGKPVYGLTVGV 59 Query: 83 GWNKDLEISP----------DFFEAYNRNLLLSHSAGVKPEADTDVVRAVMLARLNGLLT 132 G NKD ++ D ++N + L +HSAGV A + R + RLN +L Sbjct: 60 GLNKDHKLFDANGKLSDAVLDASRSFNFSTLRAHSAGVGEAAPIRLTRVALAVRLNTMLA 119 Query: 133 GAAGVQPELAGRYADFLNLGIHPVLPLRGSVGAADITSISHIGLAMIGEGEAHMDGSRVP 192 G GVQPE+A Y +LN G+ PV+P +G+VG ADI SH+GLAMIGE E G RV Sbjct: 120 GQTGVQPEVAELYEAYLNKGLTPVIPSKGTVGEADILLSSHVGLAMIGEWEVFYKGQRVS 179 Query: 193 ALEALKAAGLAPLRLGPKDGLAVVXXXXXXXXXXXXXXYDMEKLLETADRIYALSLEAIR 252 + EA+ AG+ PL KD L+++ + ++LL + ++ LSLE + Sbjct: 180 SKEAMADAGITPLVPMGKDALSILSNNAFAVAYAMQGYREAKQLLSVSPTVFGLSLEGLN 239 Query: 253 GNVSPLDEAVHRKRPFPGQLKSLGIVRSSLAGSDLWTDYNPESLQDPLSFRDACQIHGAA 312 GNV+P + RPFP + + L GS LW + LQDPLSFR A Sbjct: 240 GNVAPFLPQTNDIRPFPYIKTATSDILKQLDGSYLWQLNDERPLQDPLSFRTTAYTFAGA 299 Query: 313 RDAHDFTSRQLVLHLNSSDDNPCVL------------------SDEQRIVSCANYDPLSW 354 A + + +N SDDNP V+ + + N++PL Sbjct: 300 EQALASLDEVINIQINHSDDNPAVIVGASNQYAQFPQVAKYVVEGQGGVFPTTNFEPLPV 359 Query: 355 TLGFEMLGSALHHVSKSSCHRIIKLGDPRFTGLTRFLTADTSRSVGFCTVQKTATSLDAE 414 L + L AL HVS +S R I L D FT LTRFL+A ++ F +QK + Sbjct: 360 ALAVQNLSVALTHVSHNSVMRTIHLSDEHFTKLTRFLSAPENQGHAFGAIQKAFVDMQVR 419 Query: 415 IRHLSNPVSADYLSLAGEIEDHAANSPYVVSKTREILDRLTYILAIELLHAAQAVDLRGG 474 + L+ PVS D +S+AG IED N +I+D I +ELLH+ QA+DLR Sbjct: 420 NKQLATPVSFDGISIAGGIEDTFTNLKLASDNLIQIVDNTRVIYGLELLHSTQAIDLRKQ 479 Query: 475 ----LTLGHASWAGYKSLRRAVPFLSEDRNQSEDIE 506 L LG A+ A YK+ R VPF+++DR + DI+ Sbjct: 480 ANPELQLGKATQAMYKAYRAKVPFVAKDRPFTPDIQ 515 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 749,204,338 Number of extensions: 29808283 Number of successful extensions: 69072 Number of sequences better than 1.0e-05: 346 Number of HSP's gapped: 68198 Number of HSP's successfully gapped: 346 Length of query: 531 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 401 Effective length of database: 404,274,708 Effective search space: 162114157908 Effective search space used: 162114157908 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)