BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0032.p (554 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_657554.1| Urocanase, Urocanase [Bacillus anthracis A2012]... 567 e-160 ref|NP_242849.1| uroconase (urocanate hydratase) [Bacillus halod... 566 e-160 ref|NP_833379.1| Urocanate hydratase [Bacillus cereus ATCC 14579... 563 e-159 ref|NP_979976.1| urocanate hydratase [Bacillus cereus ATCC 10987... 563 e-159 ref|NP_419773.1| urocanate hydratase [Caulobacter crescentus CB1... 560 e-158 >ref|NP_657554.1| Urocanase, Urocanase [Bacillus anthracis A2012] ref|NP_845974.1| urocanate hydratase [Bacillus anthracis str. Ames] sp|Q81Y46|HUTU_BACAA Urocanate hydratase (Urocanase) (Imidazolonepropionate hydrolase) gb|AAP27460.1| urocanate hydratase [Bacillus anthracis str. Ames] Length = 552 Score = 567 bits (1461), Expect = e-160, Method: Compositional matrix adjust. Identities = 280/540 (51%), Positives = 376/540 (69%), Gaps = 2/540 (0%) Query: 4 ITAARGGALRCKGWRQEALLRMLENVL--ENGENQRELIVYASLGKAARNWPSYLAIVDT 61 I A RG L+ KGW QEA LRML N L E E EL+VY +G+AARNW SY AIVD+ Sbjct: 8 IRAPRGTELQTKGWVQEAALRMLMNNLDPEVAEKPEELVVYGGIGRAARNWESYQAIVDS 67 Query: 62 LKRLEEDETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGG 121 LK LE DETL++QSGKP+ +FK+H AP V++AN NLV +WA ++F EL+ KGL+++G Sbjct: 68 LKTLESDETLLVQSGKPVAIFKSHEDAPRVLLANSNLVPKWANWDHFRELEKKGLMMYGQ 127 Query: 122 LTAAAWQYIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGA 181 +TA +W YIG+QG++QGTYE F AR HFGG+L+G LTAGLGGMGGAQPLA T+ G Sbjct: 128 MTAGSWIYIGTQGILQGTYETFGEAARQHFGGSLKGTLTLTAGLGGMGGAQPLAVTMNGG 187 Query: 182 AILCVEVAEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPEL 241 ++ ++V + ID+RI Y T+SL+EALA + E +S+GLLGNAAE+ PEL Sbjct: 188 VVIAIDVDKRSIDRRIEKRYCDMYTESLEEALAVANEYKEKKEPISIGLLGNAAEILPEL 247 Query: 242 LRRGVQPDVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAML 301 ++R + PD+VTDQTSAHD L GYIP GYT AA R+EDP R + S+ V+AML Sbjct: 248 VKRNITPDLVTDQTSAHDPLNGYIPVGYTLEEAAKLREEDPERYVQLSKESMTKHVEAML 307 Query: 302 QFKERGAIVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQ 361 +E+GAI FD GNNIR A G+ +AF+ F AF+RPLF + GPFRW+ALSG+P+ Sbjct: 308 AMQEKGAITFDYGNNIRQVAFDEGLKNAFDFPGFVPAFIRPLFCEGKGPFRWVALSGDPE 367 Query: 362 DIRIIDELILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVRE 421 DI DE+IL+EF+DNE + NWI++A + F+GLP+RI W G+ +R++ +NE+V Sbjct: 368 DIYKTDEVILREFADNEHLCNWIRMARQQVEFQGLPSRICWLGYGERAKFGRIINEMVAN 427 Query: 422 GRLAGPVAFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSG 481 G L+ P+ RDHLD ++A PN TE MKDGSD+V+DWP+LNA++N + A V++H G Sbjct: 428 GELSAPIVIGRDHLDCGSVASPNRETEAMKDGSDSVADWPILNALINSVNGASWVSVHHG 487 Query: 482 GGGYAGYMTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEID 541 GG GY AG+ IVADGT + R+ L +D G+GV+R+ DAGY+ ++ A K ++ Sbjct: 488 GGVGMGYSLHAGMVIVADGTEAAAKRIERVLTSDPGMGVVRHVDAGYDLAVETAKEKGVN 547 >ref|NP_242849.1| uroconase (urocanate hydratase) [Bacillus halodurans] sp|Q9KBE5|HUTU_BACHD Urocanate hydratase (Urocanase) (Imidazolonepropionate hydrolase) pir||G83897 uroconase (urocanate hydratase) hutU [imported] - Bacillus halodurans (strain C-125) dbj|BAB05702.1| uroconase (urocanate hydratase) [Bacillus halodurans] Length = 559 Score = 566 bits (1458), Expect = e-160, Method: Compositional matrix adjust. Identities = 286/537 (53%), Positives = 375/537 (69%), Gaps = 2/537 (0%) Query: 4 ITAARGGALRCKGWRQEALLRMLENVL--ENGENQRELIVYASLGKAARNWPSYLAIVDT 61 I A +G L CKGW QEA+LRML N L E E ELIVY +GKAARNW ++ AIV+ Sbjct: 9 IQAKKGIELECKGWEQEAVLRMLYNNLDPEVAEKPDELIVYGGIGKAARNWAAFEAIVEQ 68 Query: 62 LKRLEEDETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGG 121 L+ LE+DETL+IQSGKP+G FKTH QAP V+++N LV +WA E+F EL+ +GL+++G Sbjct: 69 LRTLEKDETLLIQSGKPVGRFKTHEQAPRVLLSNSVLVPKWAHWEHFNELEKQGLMMYGQ 128 Query: 122 LTAAAWQYIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGA 181 +TA +W YIGSQG++QGTYE F +AR HFGG+L+G LTAGLGGMGGAQPLA T+ G Sbjct: 129 MTAGSWIYIGSQGILQGTYETFAELARQHFGGSLKGTLTLTAGLGGMGGAQPLAVTMNGG 188 Query: 182 AILCVEVAEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPEL 241 ++ V+V RI+KRIA Y R T+SLD+ALAW A+ GE +S+GL+GNAA ++ E+ Sbjct: 189 VVIGVDVDRSRIEKRIATKYTDRITESLDQALAWASEAKAKGEPLSIGLVGNAAHLHHEI 248 Query: 242 LRRGVQPDVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAML 301 L+RGV+ DVVTDQTSAHD L GYIPEG++ A RKE+P R A A S+ V+AML Sbjct: 249 LQRGVEVDVVTDQTSAHDPLNGYIPEGHSLEGAELLRKENPQRYMALAKQSMKKHVEAML 308 Query: 302 QFKERGAIVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQ 361 F+ RGAIVFD GNNIR A G+ +AF F A++RPLF + GPFRW ALSG+P+ Sbjct: 309 AFQRRGAIVFDYGNNIRQVAKDEGLEEAFAFPGFVPAYIRPLFCEGKGPFRWAALSGDPE 368 Query: 362 DIRIIDELILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVRE 421 DI D LI + F +NE + WI +A + + F+GLPARI W G+ +R ++ + +N LVR Sbjct: 369 DIYRTDRLIKELFPENEALIRWIDIAQEKVTFQGLPARICWLGYGERMKMGLEINRLVRN 428 Query: 422 GRLAGPVAFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSG 481 G L P+ RDHLD ++A PN TE MKDGSDAV DW +LNA++N ++ +++H G Sbjct: 429 GELKAPIVIGRDHLDCGSVASPNRETEAMKDGSDAVGDWAILNALINTAAGGSWISVHHG 488 Query: 482 GGGYAGYMTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANK 538 GG GY AG+ +VADGT ++ RLR L D G+G++R+ADAGYE + A K Sbjct: 489 GGVGMGYSLHAGMVVVADGTENAEKRLRRVLTTDPGMGIIRHADAGYELAKQVAREK 545 >ref|NP_833379.1| Urocanate hydratase [Bacillus cereus ATCC 14579] sp|Q81AC7|HUTU_BACCR Urocanate hydratase (Urocanase) (Imidazolonepropionate hydrolase) gb|AAP10580.1| Urocanate hydratase [Bacillus cereus ATCC 14579] Length = 552 Score = 563 bits (1452), Expect = e-159, Method: Compositional matrix adjust. Identities = 279/540 (51%), Positives = 374/540 (69%), Gaps = 2/540 (0%) Query: 4 ITAARGGALRCKGWRQEALLRMLENVL--ENGENQRELIVYASLGKAARNWPSYLAIVDT 61 I A RG L+ KGW QEA LRML N L E E EL+VY +G+AARNW SY AIVD+ Sbjct: 8 IRAPRGTELQTKGWVQEAALRMLMNNLDPEVAEKPEELVVYGGIGRAARNWESYQAIVDS 67 Query: 62 LKRLEEDETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGG 121 LK LE DETL++QSGKP+ +FK+H AP V++AN NLV +WA ++F EL+ KGL+++G Sbjct: 68 LKTLESDETLLVQSGKPVAIFKSHEDAPRVLLANSNLVPKWANWDHFRELEKKGLMMYGQ 127 Query: 122 LTAAAWQYIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGA 181 +TA +W YIG+QG++QGTYE F AR HF G+L+G LTAGLGGMGGAQPLA T+ G Sbjct: 128 MTAGSWIYIGTQGILQGTYETFGEAARQHFDGSLKGTLTLTAGLGGMGGAQPLAVTMNGG 187 Query: 182 AILCVEVAEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPEL 241 ++ ++V + ID+RI Y T+SL+EAL + E +S+GLLGNAAE+ PEL Sbjct: 188 VVIAIDVDKRSIDRRIEKRYCDMYTESLEEALTVANEYKEKREPISIGLLGNAAEILPEL 247 Query: 242 LRRGVQPDVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAML 301 ++R + PD+VTDQTSAHD L GYIP GYT AA R+EDP R + S+ V+AML Sbjct: 248 VKRNITPDLVTDQTSAHDPLNGYIPVGYTLEEAAKLREEDPERYVQLSKESMTKHVEAML 307 Query: 302 QFKERGAIVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQ 361 +E+GAI FD GNNIR A G+ +AF+ F AF+RPLF + GPFRW+ALSG+P+ Sbjct: 308 AMQEKGAITFDYGNNIRQVAFDEGLKNAFDFPGFVPAFIRPLFCEGKGPFRWVALSGDPE 367 Query: 362 DIRIIDELILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVRE 421 DI DE+IL+EF+DNE + NWI++A + F+GLP+RI W G+ +R++ +NE+V Sbjct: 368 DIYKTDEVILREFADNEHLCNWIRMARQQVEFQGLPSRICWLGYGERAKFGRIINEMVAN 427 Query: 422 GRLAGPVAFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSG 481 G L+ P+ RDHLD ++A PN TE MKDGSDAV+DWP+LNA++N + A V++H G Sbjct: 428 GELSAPIVIGRDHLDCGSVASPNRETEAMKDGSDAVADWPILNALINSVNGASWVSVHHG 487 Query: 482 GGGYAGYMTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEID 541 GG GY AG+ IVADGT + R+ L +D G+GV+R+ DAGY+ ++ A K ++ Sbjct: 488 GGVGMGYSLHAGMVIVADGTEAAAKRIERVLTSDPGMGVVRHVDAGYDLAVETAKEKGVN 547 >ref|NP_979976.1| urocanate hydratase [Bacillus cereus ATCC 10987] gb|AAS42584.1| urocanate hydratase [Bacillus cereus ATCC 10987] Length = 552 Score = 563 bits (1451), Expect = e-159, Method: Compositional matrix adjust. Identities = 279/540 (51%), Positives = 373/540 (69%), Gaps = 2/540 (0%) Query: 4 ITAARGGALRCKGWRQEALLRMLENVL--ENGENQRELIVYASLGKAARNWPSYLAIVDT 61 I A RG L+ KGW QEA LRML N L E E EL+VY +G+AARNW SY AIVD+ Sbjct: 8 IRAPRGTELQTKGWVQEAALRMLMNNLDPEVAEKPEELVVYGGIGRAARNWESYNAIVDS 67 Query: 62 LKRLEEDETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGG 121 LK LE DETL++QSGKP+ +FK+H AP V++AN NLV +WA +F EL+ KGL+++G Sbjct: 68 LKTLESDETLLVQSGKPVAIFKSHEDAPRVLLANSNLVPKWANWNHFRELEKKGLMMYGQ 127 Query: 122 LTAAAWQYIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGA 181 +TA +W YIG+QG++QGTYE F AR HFGG+L+G LTAGLGGMGGAQPLA T+ G Sbjct: 128 MTAGSWIYIGTQGILQGTYETFGEAARQHFGGSLKGTLTLTAGLGGMGGAQPLAVTMNGG 187 Query: 182 AILCVEVAEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPEL 241 ++ ++V + ID+RI Y T+SL+EAL + E +S+GLLGNAAE+ PEL Sbjct: 188 VVIAIDVDKRSIDRRIEKRYCDMYTESLEEALTVANEYKEKKEPISIGLLGNAAEILPEL 247 Query: 242 LRRGVQPDVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAML 301 ++R + PD+VTDQTSAHD L GYIP GYT AA R+EDP R + S+ V+AML Sbjct: 248 VKRNITPDLVTDQTSAHDPLNGYIPVGYTLEEAAKLREEDPERYVQLSKESMTKHVEAML 307 Query: 302 QFKERGAIVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQ 361 + +GAI FD GNNIR A G+ +AF+ F AF+RPLF + GPFRW+ALSG+P+ Sbjct: 308 AMQAKGAITFDYGNNIRQVAFDEGLKNAFDFPGFVPAFIRPLFCEGKGPFRWVALSGDPE 367 Query: 362 DIRIIDELILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVRE 421 DI DE+IL+EF+DNE + NWI++A + F+GLP+RI W G+ +R++ +NE+V Sbjct: 368 DIYKTDEVILREFADNEHLCNWIRMARQQVEFQGLPSRICWLGYGERAKFGRIINEMVAN 427 Query: 422 GRLAGPVAFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSG 481 G L+ P+ RDHLD ++A PN TE MKDGSDAV+DWP+LNA++N + A V++H G Sbjct: 428 GELSAPIVIGRDHLDCGSVASPNRETEAMKDGSDAVADWPILNALINSVNGASWVSVHHG 487 Query: 482 GGGYAGYMTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEID 541 GG GY AG+ IVADGT + R+ L +D G+GV+R+ DAGY+ ++ A K ++ Sbjct: 488 GGVGMGYSLHAGMVIVADGTEAAAKRIERVLTSDPGMGVVRHVDAGYDLAVETAKEKGVN 547 >ref|NP_419773.1| urocanate hydratase [Caulobacter crescentus CB15] sp|Q9A9M1|HUTU_CAUCR Urocanate hydratase (Urocanase) (Imidazolonepropionate hydrolase) pir||A87368 urocanate hydratase [imported] - Caulobacter crescentus gb|AAK22941.1| urocanate hydratase [Caulobacter crescentus CB15] Length = 554 Score = 560 bits (1443), Expect = e-158, Method: Compositional matrix adjust. Identities = 284/540 (52%), Positives = 371/540 (68%), Gaps = 2/540 (0%) Query: 4 ITAARGGALRCKGWRQEALLRMLENVLEN--GENQRELIVYASLGKAARNWPSYLAIVDT 61 I A G CK W EA LRML N L E EL+VY +G+AAR+W SY IV+T Sbjct: 10 IRPATGPERTCKSWLTEAALRMLMNNLHPDVAERPEELVVYGGIGRAARDWESYDKIVET 69 Query: 62 LKRLEEDETLVIQSGKPIGVFKTHRQAPLVVMANCNLVGRWATSENFYELQDKGLIIWGG 121 L+RLE+DETL++QSGKP+GVF+TH AP V++AN NLV RWA+ E+F EL KGL ++G Sbjct: 70 LRRLEDDETLLVQSGKPVGVFRTHADAPRVLIANSNLVPRWASWEHFNELDRKGLAMYGQ 129 Query: 122 LTAAAWQYIGSQGVIQGTYEIFQSIARLHFGGNLRGRFILTAGLGGMGGAQPLAGTLAGA 181 +TA +W YIG+QG++QGTYE F + R H+GG+L GR++LTAGLGGMGGAQPLA +AGA Sbjct: 130 MTAGSWIYIGAQGIVQGTYETFVEMGRQHYGGDLSGRWLLTAGLGGMGGAQPLAAVMAGA 189 Query: 182 AILCVEVAEERIDKRIAAGYLQRKTDSLDEALAWIEIARRSGESVSVGLLGNAAEVYPEL 241 A L +E I+ R+ GYL R TD ++EALAWIE + + +SVGLLGNAAE+ PEL Sbjct: 190 ACLAIECQPSSIEMRLRTGYLDRSTDKVEEALAWIEESCAAKTPISVGLLGNAAELLPEL 249 Query: 242 LRRGVQPDVVTDQTSAHDLLYGYIPEGYTPASAAAARKEDPARLQAAAGASIALEVKAML 301 +RGV+PD++TDQTSAHD + GY+P G++ A R +DP ++ AA AS+A VKAML Sbjct: 250 FKRGVRPDLLTDQTSAHDPVNGYLPAGWSLERWHAMRDQDPPQVAEAAKASMAAHVKAML 309 Query: 302 QFKERGAIVFDNGNNIRSQAVQHGVPDAFEIDIFTEAFLRPLFAKAIGPFRWIALSGEPQ 361 F+ G D GNNIR A++ GV +AF+ F A++RPLF + +GPFRW ALSG+P+ Sbjct: 310 DFQAAGVPTVDYGNNIRQMALEEGVTNAFDFPGFVPAYIRPLFCRGVGPFRWAALSGDPE 369 Query: 362 DIRIIDELILQEFSDNEIVTNWIKLANDHIPFEGLPARIGWFGHKDRSRLAVAVNELVRE 421 DI D + + DN + NW+ +A I F+GLPARI W G DR RL +A NE+V + Sbjct: 370 DIAKTDAKVKELIPDNPHLHNWLDMAGQKIRFQGLPARICWVGLGDRHRLGLAFNEMVAK 429 Query: 422 GRLAGPVAFSRDHLDAAAMAHPNIMTERMKDGSDAVSDWPLLNAMLNCSSMADLVAIHSG 481 G L P+ RDHLD+ ++A PN TE M+DGSDAVSDWPLLNA+LN +S A V++H G Sbjct: 430 GELKAPIVIGRDHLDSGSVASPNRETEAMRDGSDAVSDWPLLNALLNTASGATWVSLHHG 489 Query: 482 GGGYAGYMTSAGVTIVADGTADSGLRLRTALDNDTGLGVLRYADAGYETSLAEAANKEID 541 GG G+ AG+ IV DGT + R+ L ND GV+R+ADAGY+ +LA A K +D Sbjct: 490 GGVGMGFSQHAGMVIVCDGTEAAAKRVARVLWNDPATGVMRHADAGYDIALACAREKGLD 549 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 814,032,547 Number of extensions: 34308675 Number of successful extensions: 90757 Number of sequences better than 1.0e-05: 175 Number of HSP's gapped: 90539 Number of HSP's successfully gapped: 175 Length of query: 554 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 423 Effective length of database: 401,559,609 Effective search space: 169859714607 Effective search space used: 169859714607 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)