BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0031.p (455 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00109144.1| COG2141: Coenzyme F420-dependent N5,N10-methy... 457 e-127 ref|ZP_00160953.1| hypothetical protein Avar245801 [Anabaena var... 455 e-127 ref|ZP_00160762.1| hypothetical protein Avar226201 [Anabaena var... 448 e-124 ref|ZP_00109145.1| COG2141: Coenzyme F420-dependent N5,N10-methy... 446 e-124 ref|ZP_00086468.1| COG2141: Coenzyme F420-dependent N5,N10-methy... 441 e-122 >ref|ZP_00109144.1| COG2141: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases [Nostoc punctiforme] Length = 455 Score = 457 bits (1175), Expect = e-127, Method: Compositional matrix adjust. Identities = 238/443 (53%), Positives = 299/443 (67%), Gaps = 9/443 (2%) Query: 6 RQLALGAMMFFPAGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADELYVW 65 R+L LGA + G HIS+WRHP S+A A L+F++YK I QTAERG FD F AD + Sbjct: 5 RKLRLGAFIQ-ATGHHISAWRHPDSQADAGLNFEHYKEITQTAERGLFDAVFLADSPSLQ 63 Query: 66 DRFESGISHANS--IRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASLDHLS 123 D E + N + EP TL SALA +T HIG AT STTY +PY +ARK ASLD+LS Sbjct: 64 DNSEPEVLKRNGKLAKFEPVTLFSALAPLTKHIGFVATASTTYEEPYTLARKFASLDYLS 123 Query: 124 GGRAAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVFDKES 183 GRAAWN+VT+ + A NFG E H +H RYERA+EFV V +GLWDSWE+DA + DKES Sbjct: 124 NGRAAWNVVTTGNENAAGNFGLEHHPEHSQRYERAEEFVEVVKGLWDSWEDDAFIRDKES 183 Query: 184 GYFADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEIVFAP 243 G + D DKLH L+++GKHF V+GPLN+ RPPQGYPV++QAGASE G+++AA TAE++F Sbjct: 184 GVYFDPDKLHTLNYKGKHFSVQGPLNVGRPPQGYPVIVQAGASEVGRDLAAQTAEVIFTA 243 Query: 244 GGTLAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLTPDRL 303 TL +G+ YAD+KGRLAKYGR D+LKI P + PVIG TEE+A EK +++L + Sbjct: 244 NQTLEDGQEFYADVKGRLAKYGRQLDDLKIMPGVFPVIGRTEEEAQEKYEFLQSLIHPSV 303 Query: 304 ALDLL-SHYIGTDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQLAKRV 362 A LL + Y G D+S Y +S+ LL ++ + NLTL QL V Sbjct: 304 AWGLLKTRYKGIDLSGYSLDDLAPPLPSDTNGGKSRLKLLT--DLVNRGNLTLRQLYLSV 361 Query: 363 AVKRG---IAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQRGLL 419 A RG I G+PE IAD +EEWF + AADGFNI LP LDDFVNLV+P+LQ+RGL Sbjct: 362 ATARGHRTIIGSPESIADQLEEWFSNGAADGFNIMPPILPTGLDDFVNLVIPVLQKRGLF 421 Query: 420 RTEYEGTTLRAHLGLNTPASRYA 442 RTEYEG TLR +LGL P + +A Sbjct: 422 RTEYEGNTLRENLGLRRPGNGFA 444 >ref|ZP_00160953.1| hypothetical protein Avar245801 [Anabaena variabilis ATCC 29413] Length = 457 Score = 455 bits (1171), Expect = e-127, Method: Compositional matrix adjust. Identities = 242/459 (52%), Positives = 311/459 (67%), Gaps = 14/459 (3%) Query: 5 KRQLALGAMMFFP-AGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADELY 63 K+Q+ LGA F P +G H+++WRHP S+A L+F +YKR+ QTAERG+FDM F AD L Sbjct: 4 KKQIKLGA--FLPGSGHHVAAWRHPESQADGGLNFQHYKRLAQTAERGKFDMVFLADGLA 61 Query: 64 VWDRFESGISHANS----IRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASL 119 VWDR + + + S + EP TLLSAL+ VT +IGL AT STTY +P+HIARK ASL Sbjct: 62 VWDRGQGKEAFSRSGQFSVHFEPLTLLSALSVVTENIGLVATASTTYEEPFHIARKFASL 121 Query: 120 DHLSGGRAAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVF 179 D+LSGGRA WN+VTS + A+NF KE HL+H +RYERA EF+ VT LWDSWE+DA + Sbjct: 122 DYLSGGRAGWNVVTSSAEAAAQNFSKEEHLEHAIRYERAIEFLQVTSKLWDSWEDDAFIL 181 Query: 180 DKESGYFADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEI 239 DKESG + + DKLH + +GKHF V+GPLN+ RP QGYPV++QAG+S GKE+AA TAE Sbjct: 182 DKESGIYFEHDKLHIPNHKGKHFSVRGPLNVARPIQGYPVIVQAGSSLPGKELAAQTAEA 241 Query: 240 VFAPGGTLAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLT 299 +F TLAE +A Y+D+KG+LAKYGR+ D LKI P + PVIG TEE+A EK V+++L Sbjct: 242 IFTAQQTLAEAQAFYSDVKGKLAKYGRSPDHLKIMPGVFPVIGRTEEEAKEKYQVLQDLI 301 Query: 300 -PDRLALDLLSHYIGTDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQL 358 P L G D+S Y N K+ L I + +ENLT+ QL Sbjct: 302 HPSVGLGLLSGLVGGHDLSQYPLDGPLPDLPDT---NAGKSRLQLITDLARRENLTIRQL 358 Query: 359 AKRVAVKRG---IAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQ 415 +A RG I GTPEQIAD +E+WF + AADGFNI LPG LD+FV+LVVP LQ+ Sbjct: 359 YLWIAGARGHRTILGTPEQIADQLEDWFGNDAADGFNIMPPWLPGGLDEFVDLVVPELQR 418 Query: 416 RGLLRTEYEGTTLRAHLGLNTPASRYAKIGISPGAGSEQ 454 RGL RTEYEG TLR +LGL P +++ ++ AG+ + Sbjct: 419 RGLFRTEYEGRTLRENLGLPRPVNQFTQVASPELAGASK 457 >ref|ZP_00160762.1| hypothetical protein Avar226201 [Anabaena variabilis ATCC 29413] Length = 454 Score = 448 bits (1152), Expect = e-124, Method: Compositional matrix adjust. Identities = 236/443 (53%), Positives = 298/443 (67%), Gaps = 8/443 (1%) Query: 5 KRQLALGAMMFFPAGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADELYV 64 KRQ LGA + G HIS+WRHP ++ A +FD+YK I QTAERG FD F AD V Sbjct: 4 KRQFRLGAFIQ-ATGHHISAWRHPDAQIDAGSNFDHYKEITQTAERGLFDAVFLADSPGV 62 Query: 65 WD-RFESGISHANSIRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASLDHLS 123 W E+ + EP TL SAL++VT +IG +T STTY PY +ARK ASLDHLS Sbjct: 63 WGGEPETQKRNGKLAHFEPVTLFSALSSVTQNIGFISTASTTYEHPYTLARKFASLDHLS 122 Query: 124 GGRAAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVFDKES 183 GRA WN+VT+ + A NFG E H +H RYERA+EFV V +GLWDSWE+DA + D+ES Sbjct: 123 KGRAGWNVVTTGNESAAGNFGLEHHPEHSQRYERAEEFVQVVKGLWDSWEDDAFIRDRES 182 Query: 184 GYFADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEIVFAP 243 G + D DKLH L+ +GK+F VKGPLN+ RPPQGYPV++QAGASEAG+E+AA TAE++F Sbjct: 183 GIYFDTDKLHVLNHKGKYFSVKGPLNVARPPQGYPVIVQAGASEAGRELAARTAEVIFTA 242 Query: 244 GGTLAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLTPDRL 303 TLA+ + YAD+KGRLAKYGR+ D+LKI P P+IG TEE+A EK +++L + Sbjct: 243 NQTLADAQEFYADVKGRLAKYGRSPDDLKIMPGAFPIIGRTEEEAQEKYEFLQSLIHPDV 302 Query: 304 ALDLLS-HYIGTDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQLAKRV 362 A +L +Y G D+S Y N+S+ L+K + + +LTL QL + Sbjct: 303 AWGILKRYYKGLDLSKYSLDDIAPELPGDTNNNKSRLKLVK--DLATRGSLTLRQLYLAL 360 Query: 363 AVKRG---IAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQRGLL 419 A RG I GTPE IAD +EEWF + AADGFNI LP LD+FVNLVVPILQ+RGL Sbjct: 361 ATARGHRTILGTPESIADQLEEWFNNSAADGFNIMPPILPTGLDEFVNLVVPILQKRGLF 420 Query: 420 RTEYEGTTLRAHLGLNTPASRYA 442 RTEYEGTTLR +LGL P +++A Sbjct: 421 RTEYEGTTLRENLGLRRPENQFA 443 >ref|ZP_00109145.1| COG2141: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases [Nostoc punctiforme] Length = 454 Score = 446 bits (1147), Expect = e-124, Method: Compositional matrix adjust. Identities = 230/442 (52%), Positives = 299/442 (67%), Gaps = 8/442 (1%) Query: 6 RQLALGAMMFFPAGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADELYVW 65 R+ LGA + G H+SSWRHP ++A A L+F++YK I QTAERG FD F AD VW Sbjct: 5 RKFRLGAFVQ-ATGHHVSSWRHPDAQADAGLNFEHYKEITQTAERGLFDAVFLADSPGVW 63 Query: 66 D-RFESGISHANSIRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASLDHLSG 124 E+ + EP TL SAL++VT +IG +T STTY +PY +ARK ASLD+LS Sbjct: 64 GGSPETQYRNGKMAHFEPVTLFSALSSVTKNIGFISTASTTYEEPYTLARKFASLDYLSN 123 Query: 125 GRAAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVFDKESG 184 GRA WN+VT+ + ARNFG E H +H RYERA+EFV V +GLWDSWE+DA + DKESG Sbjct: 124 GRAGWNVVTTGNENAARNFGLEHHPEHSQRYERAEEFVEVVKGLWDSWEDDAFIRDKESG 183 Query: 185 YFADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEIVFAPG 244 + D DKLH L+ +GK+F V+GPLN+ RPPQGYPV++QAGASE+G+++AA TAE++F Sbjct: 184 VYFDPDKLHTLNHKGKYFSVQGPLNVGRPPQGYPVIVQAGASESGRDLAARTAEVIFTAN 243 Query: 245 GTLAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLTPDRLA 304 TLA+ + YAD+KGRLA+YGR+ D+LKI P P+IG TE +A EK +++L +A Sbjct: 244 QTLADAQEFYADVKGRLAQYGRSPDDLKIMPGAFPIIGRTEAEAQEKYEFLQSLIHPDVA 303 Query: 305 LDLL-SHYIGTDVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQLAKRVA 363 +L ++Y G D+S Y N+S+ L +R + + LTL QL +A Sbjct: 304 WGILKNYYKGVDLSKYSLDDVAPELPSDTNTNKSRLKL--VRDLATRGTLTLRQLYLSLA 361 Query: 364 VKRG---IAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQRGLLR 420 RG I GTPE IAD +EEWF + AAD FNI LP LD+FVNLVVPILQ+RGL R Sbjct: 362 TARGHRTILGTPETIADQLEEWFNNGAADAFNIMPPILPTELDEFVNLVVPILQKRGLFR 421 Query: 421 TEYEGTTLRAHLGLNTPASRYA 442 TEYEG+TLR +LGL P +R+A Sbjct: 422 TEYEGSTLRENLGLRRPVNRFA 443 >ref|ZP_00086468.1| COG2141: Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases [Pseudomonas fluorescens PfO-1] Length = 498 Score = 441 bits (1135), Expect = e-122, Method: Compositional matrix adjust. Identities = 227/445 (51%), Positives = 294/445 (66%), Gaps = 8/445 (1%) Query: 2 SDVKRQLALGAMMFFPAGEHISSWRHPGSEAAALLDFDYYKRIVQTAERGRFDMFFYADE 61 S++ RQL LGA + G H+++WRHP A A LDF +YKR+ Q AE +FD F AD Sbjct: 58 SEMSRQLKLGAFLM-ATGHHVAAWRHPNVPANAGLDFAHYKRLAQIAEAAKFDTLFVADS 116 Query: 62 LYVWDRFESGISHANSIRPEPFTLLSALAAVTTHIGLAATISTTYNDPYHIARKLASLDH 121 + + + A S EP TLLSAL+AVT HIGL AT +T+YN+PYH+ARK ASLDH Sbjct: 117 VAAPTQ-DIASRMARSDHFEPLTLLSALSAVTEHIGLIATATTSYNEPYHVARKFASLDH 175 Query: 122 LSGGRAAWNIVTSQTDEEARNFGKERHLQHELRYERAKEFVAVTQGLWDSWEEDACVFDK 181 LSGGRA WN+VTS EA+NFG++ H+ H RY RA+EF V GLWDSW++DA + DK Sbjct: 176 LSGGRAGWNLVTSDNAAEAQNFGRDEHIGHAERYSRAREFHQVVTGLWDSWQDDAFIRDK 235 Query: 182 ESGYFADKDKLHNLDFEGKHFKVKGPLNITRPPQGYPVLIQAGASEAGKEVAAATAEIVF 241 SG + D +KLH L+ G+HF+VKGPLN+ R PQG PV++QAG+SE G+E+AA TAE+VF Sbjct: 236 ASGAYYDPEKLHVLNHVGEHFRVKGPLNVARSPQGQPVIVQAGSSETGRELAAQTAEVVF 295 Query: 242 APGGTLAEGRALYADIKGRLAKYGRTADELKIFPALMPVIGITEEDAAEKLSVIENLTPD 301 +LA +A YAD+KGRL KYGR+AD LKI P + V+G TE +A EK + L Sbjct: 296 TAQTSLASAKAFYADLKGRLPKYGRSADSLKIMPGVFVVVGQTEAEAQEKCETFQQLVEP 355 Query: 302 RLALDLLSHYIGT-DVSSYXXXXXXXXXXXXXGFNQSKTGLLKIRSILEQENLTLLQLAK 360 + + LL +G D+S Y QS+ LL + +E+LTL +L + Sbjct: 356 EVGIALLGRMLGNFDLSKYPLDGPLPELPLTDSGQQSRQKLLT--ELAGREHLTLAELGR 413 Query: 361 RVAVKRG---IAGTPEQIADHIEEWFKSKAADGFNIAFTHLPGALDDFVNLVVPILQQRG 417 R+A RG + GTP QIAD ++EWF+ AADGFN+ HLPG L+DF N VVP LQ+RG Sbjct: 414 RIAGGRGHYSVVGTPAQIADRLQEWFEQGAADGFNVLVPHLPGGLEDFANGVVPELQRRG 473 Query: 418 LLRTEYEGTTLRAHLGLNTPASRYA 442 L RTEYEG TLR +LGL P +R+A Sbjct: 474 LFRTEYEGRTLRQNLGLARPGNRFA 498 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 674,271,528 Number of extensions: 27845316 Number of successful extensions: 66353 Number of sequences better than 1.0e-05: 342 Number of HSP's gapped: 65819 Number of HSP's successfully gapped: 357 Length of query: 455 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 326 Effective length of database: 406,989,807 Effective search space: 132678677082 Effective search space used: 132678677082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)