BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0030.p (312 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00182740.1| hypothetical protein Exigu193601 [Exiguobacte... 203 4e-51 ref|ZP_00180948.1| hypothetical protein Mther101101 [Moorella th... 201 2e-50 ref|ZP_00103660.1| COG1174: ABC-type proline/glycine betaine tra... 194 2e-48 ref|NP_349452.1| Proline/glycine betaine ABC-type transport syst... 190 4e-47 ref|NP_561474.1| probable glycine betaine/carnitine/choline ABC ... 185 1e-45 >ref|ZP_00182740.1| hypothetical protein Exigu193601 [Exiguobacterium sp. 255-15] Length = 464 Score = 203 bits (517), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 121/273 (44%), Positives = 169/273 (61%), Gaps = 4/273 (1%) Query: 43 SDTIVLATKGFAESDILANAFKLLIEEDTDLKTEVK-KLDNT-LLWNAIDTGDVDAYVEY 100 +D IV+ +K F E IL N LIE+ TDL+ E + L T + + A++TG++DAYVEY Sbjct: 192 TDKIVIGSKNFTEQLILGNMLADLIEDKTDLEVERQLNLGGTKVAYGALETGEIDAYVEY 251 Query: 101 SGTALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAVRKETAEKYGL 160 +GT LI++LK E DP+ ++ V + EK+ + LEP+GFNNTY A+ KE +KY L Sbjct: 252 TGTLLIDVLKQKLETDPEAVYKKVGKMIPEKSQVDVLEPIGFNNTYALAMTKEDIKKYDL 311 Query: 161 TTASQLAEKSGELIFGSSEEFLNRADAWPYVENIYK-PKFKEIKSIQNKSLQYQAIDQKL 219 T S +A S LI GS+ EF NR D + ++ Y KFK+++ + + L+Y A+ Sbjct: 312 KTVSDMAGVSNRLILGSTIEFANREDGYLGLKEKYPDMKFKDVQPV-DGGLRYTALTNGK 370 Query: 220 IDVMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKETLNKLAGKID 279 +V+ +STD L L LEDDK F PY A PIVR +TLEEHPELKE LN L KI Sbjct: 371 TNVIDSFSTDGLLKKFDLTVLEDDKEFFPPYYAVPIVRQETLEEHPELKEVLNLLKDKIT 430 Query: 280 EATMQALNAEVEQEQKPAIDVAKEWLKSQALIK 312 + MQ LN + + E++ DVA+E+L + LIK Sbjct: 431 DKKMQELNFKADVEKERPEDVAREFLIEEELIK 463 >ref|ZP_00180948.1| hypothetical protein Mther101101 [Moorella thermoacetica ATCC 39073] Length = 268 Score = 201 bits (511), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 113/269 (42%), Positives = 166/269 (61%), Gaps = 3/269 (1%) Query: 46 IVLATKGFAESDILANAFKLLIEEDTDLKTEVK-KLDNTLL-WNAIDTGDVDAYVEYSGT 103 +V+ +K F E+ +L LIE TDLK E K L TL+ +NA+ GD+D Y +Y+GT Sbjct: 1 MVVGSKDFTENILLGEIMAQLIEAHTDLKVERKLNLGGTLVNFNALKKGDLDLYADYTGT 60 Query: 104 ALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAVRKETAEKYGLTTA 163 L+ ILK D DP +A+ V E+ L L+P GFNNTY AV +E A + L Sbjct: 61 GLVAILKRDVINDPQEAYDAVQKAYNEQFKLKWLKPFGFNNTYALAVPEEVARQRNLQKI 120 Query: 164 SQLAEKSGELIFGSSEEFLNRADAWPYVENIYKPKFKEIKSIQNKSLQYQAIDQKLIDVM 223 S L +GE++ G+ +EF NR D + + Y FK K ++ L+Y+AI K++DV+ Sbjct: 121 SDLKSVAGEMVLGAEQEFFNRPDGYDGLIVTYGLNFKSTKQMET-GLKYEAIHNKMVDVI 179 Query: 224 LPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKETLNKLAGKIDEATM 283 ++TD QL+T L LEDDK F PY A P+VR TLE++P+L+E LNKLAG++++ M Sbjct: 180 DAFATDGQLITYKLKILEDDKQFFPPYFAAPLVRMDTLEKYPQLEEVLNKLAGQLNDDEM 239 Query: 284 QALNAEVEQEQKPAIDVAKEWLKSQALIK 312 + LN +V++E+K VA+++L + LIK Sbjct: 240 RQLNYQVDEEKKEVAQVARDFLLKKGLIK 268 >ref|ZP_00103660.1| COG1174: ABC-type proline/glycine betaine transport systems, permease component [Desulfitobacterium hafniense] Length = 313 Score = 194 bits (493), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 111/282 (39%), Positives = 171/282 (60%), Gaps = 6/282 (2%) Query: 33 GCSNSKASGSSDTIVLATKGFAESDILANAFKLLIEEDTDLKTE--VKKLDNTLLWNAID 90 GCS+S A+ S ++I + +K F E+ I+A+ L+E TDL + V + + W A++ Sbjct: 35 GCSSS-AAKSGESITVGSKNFTENIIIAHMMADLVEAHTDLTVDRKVNLGGSNVAWTALE 93 Query: 91 TGDVDAYVEYSGTALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAV 150 D+ Y +Y+GT + N + + +T+ +L ++NL AL LGFNNTY AV Sbjct: 94 NNDIQMYPDYTGTIVANYYQEATGTSEETMAKTL--ELTAEDNLKALGSLGFNNTYTLAV 151 Query: 151 RKETAEKYGLTTASQLAEKSGELIFGSSEEFLNRADAWPYVENIYKPKFKEIKSIQNKSL 210 KETA+KY L T S L S +L+ G EF +R D +P +++ Y FKE+K + + + Sbjct: 152 TKETAQKYNLKTYSDLVGVSEQLVLGCEFEFKDRPDGYPGLQDTYNMNFKEVKGM-DHGI 210 Query: 211 QYQAIDQKLIDVMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKET 270 ++++ + +DV+ Y+TD Q+ LV LEDD+ F PYD P+VR TL+++PEL+E Sbjct: 211 MFRSLVEGEVDVVDAYATDGQIKVFDLVILEDDREFFPPYDCLPLVRQDTLDQYPELEEI 270 Query: 271 LNKLAGKIDEATMQALNAEVEQEQKPAIDVAKEWLKSQALIK 312 LNKLAG+I+EA MQ LNA+V+ + VA ++L S LIK Sbjct: 271 LNKLAGQINEADMQELNAKVDDQGMKEDVVAHDFLVSAGLIK 312 >ref|NP_349452.1| Proline/glycine betaine ABC-type transport system, permease component fused to periplasmic component [Clostridium acetobutylicum] pir||E97250 proline/glycine betaine ABC-type transport system, permease component fused to periplasmic component CAC2849 [imported] - Clostridium acetobutylicum gb|AAK80792.1| Proline/glycine betaine ABC-type transport system, permease component fused to periplasmic component [Clostridium acetobutylicum] Length = 523 Score = 190 bits (482), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 111/271 (40%), Positives = 164/271 (60%), Gaps = 6/271 (2%) Query: 45 TIVLATKGFAESDILANAFKLLIEEDTDLKTEVKKLD---NTLLWNAIDTGDVDAYVEYS 101 IV+ +K F E IL N LIE +TD K E +KL+ ++ +NA+ +G++D YVEY+ Sbjct: 256 VIVIGSKNFNEQLILGNMVASLIEHNTDYKVE-RKLNLGGTSVAFNALKSGNIDMYVEYT 314 Query: 102 GTALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAVRKETAEKYGLT 161 GT L++I+K D K + V + + N+ L+P+GFNNTY A++K A KY + Sbjct: 315 GTGLVDIMKKPTISDEKKVYNDVKSFFDKNYNIEWLKPIGFNNTYALAMKKNVASKYNIN 374 Query: 162 TASQLAEKSGELIFGSSEEFLNRADAWPYVENIYKPKFKEIKSIQNKSLQYQAIDQKLID 221 T S L++ S +I GS+ EFLNR+D +P + N Y KF K + + L+Y ++++ Sbjct: 375 TVSDLSKVSNNMILGSTMEFLNRSDGYPGLNNKYGLKFNSTKGL-DGGLRYSSLNKNETQ 433 Query: 222 VMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKETLNKLAGKIDEA 281 V + TD L L L+DDK+ F PY A PIVR TL++ P+LK L KLA KI ++ Sbjct: 434 VTDAFLTDGLLQKFNLKVLKDDKNFFPPYYAAPIVREDTLKKFPKLKPLLMKLANKITDS 493 Query: 282 TMQALNAEVEQEQKPAIDVAKEWLKSQALIK 312 M+ALN +V+ Q P VA E+L+S+ LIK Sbjct: 494 EMRALNYKVDNGQDPQ-KVADEFLRSKHLIK 523 >ref|NP_561474.1| probable glycine betaine/carnitine/choline ABC transporter [Clostridium perfringens] dbj|BAB80264.1| probable glycine betaine/carnitine/choline ABC transporter [Clostridium perfringens str. 13] Length = 517 Score = 185 bits (469), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 109/280 (38%), Positives = 167/280 (59%), Gaps = 5/280 (1%) Query: 36 NSKASGSSDTIVLATKGFAESDILANAFKLLIEEDTDLKTEVKKLD---NTLLWNAIDTG 92 +S S D +V+ +K F E IL N + L++ TDL+ E KKL+ ++ + A++ G Sbjct: 239 SSFISSRKDKVVIGSKNFTEQLILGNMYADLVQAKTDLQVE-KKLNLGGTSVAFGALEKG 297 Query: 93 DVDAYVEYSGTALINILKLDPEFDPDKAFQTVVAQLKEKNNLTALEPLGFNNTYVFAVRK 152 DVD YV+Y+GT L+N++K + + + ++ + +++ LT +EPLGFNNTY A+ K Sbjct: 298 DVDMYVDYTGTLLVNVMKENNIDNSVDYYNSIKENMNKEHGLTVMEPLGFNNTYNIAISK 357 Query: 153 ETAEKYGLTTASQLAEKSGELIFGSSEEFLNRADAWPYVENIYKPKFKEIKSIQNKSLQY 212 E A+KY + T S L++ S E + + EF NR D ++N Y FK +KS+ + SL+Y Sbjct: 358 ELADKYKINTISDLSKYSNEFVLSPTIEFQNRQDGLVGLKNYYGMDFKNVKSL-DGSLRY 416 Query: 213 QAIDQKLIDVMLPYSTDSQLLTSGLVALEDDKHVFIPYDAFPIVRGQTLEEHPELKETLN 272 A+ + +STD L L LEDDK F+ Y A PIV +TLE++P+LK+ LN Sbjct: 417 SALSNGESQAIDAFSTDGLLKKFDLKTLEDDKKFFVNYSAVPIVNNKTLEKYPQLKDVLN 476 Query: 273 KLAGKIDEATMQALNAEVEQEQKPAIDVAKEWLKSQALIK 312 L+GKI+E M LN EV+ K +VAK +L + LI+ Sbjct: 477 SLSGKINEEKMIDLNYEVDVLGKSPEEVAKAFLIREGLIE 516 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.130 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 411,496,151 Number of extensions: 16430813 Number of successful extensions: 42619 Number of sequences better than 1.0e-05: 115 Number of HSP's gapped: 42372 Number of HSP's successfully gapped: 115 Length of query: 312 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 187 Effective length of database: 417,850,203 Effective search space: 78137987961 Effective search space used: 78137987961 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 126 (53.1 bits)