BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0027.p (379 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_349453.1| Proline/glycine betaine ABC-type transport syst... 351 2e-95 ref|ZP_00180945.1| hypothetical protein Mther100801 [Moorella th... 348 1e-94 ref|ZP_00182739.1| hypothetical protein Exigu193501 [Exiguobacte... 337 4e-91 ref|NP_781074.1| glycine/betaine transport ATP-binding protein [... 336 5e-91 ref|NP_391253.1| choline ABC transporter (ATP-binding protein) [... 334 2e-90 >ref|NP_349453.1| Proline/glycine betaine ABC-type transport system, ATPase component [Clostridium acetobutylicum] pir||F97250 proline/glycine betaine ABC-type transport system, ATPase component CAC2850 [imported] - Clostridium acetobutylicum gb|AAK80793.1| Proline/glycine betaine ABC-type transport system, ATPase component [Clostridium acetobutylicum] Length = 377 Score = 351 bits (900), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 175/377 (46%), Positives = 266/377 (70%), Gaps = 10/377 (2%) Query: 7 VEFRNVSKDYGSGSVLRNFNLTVPKGQIVTIIGPSGCGKTTLLKMINRLVEPSIGNVLVE 66 +E +N+ K + +L N NLT+ KG++VT+IGPSGCGKTT LKMINRL+ + G +L+ Sbjct: 2 IELKNIYKKVNNRIILNNVNLTINKGELVTLIGPSGCGKTTTLKMINRLIRQTSGEILIN 61 Query: 67 GTDIGTLDAVELRRDIGYVIQQIGLFPHMTIEENISIVPKLKGAGKTERKERVDALLSLI 126 G I DA++LRR+IGYVIQQ GLFPH+T+EENIS++ K++ +T+ ++V+ LL+L+ Sbjct: 62 GNKIQNEDAIKLRRNIGYVIQQTGLFPHLTVEENISMILKIEKVDQTKIDKKVEELLNLV 121 Query: 127 GLPPEQYRKRYPHELSGGQQQRIGVARALASDPSIILMDEPFSALDPISRVQLQKELINL 186 G+ PE Y+ +YP +LSGGQQQRIG+ARALA DP IILMDEPFSALDP++R QLQ E+ ++ Sbjct: 122 GMNPEDYKYKYPSQLSGGQQQRIGIARALAMDPEIILMDEPFSALDPLTRNQLQDEIFSI 181 Query: 187 NQRLHKTIVFVTHDIDEALKIADRIILLNEGEVVQNSTPEELLAHPANDFVIEFVGQERF 246 +Q L KTIVFVTHD+DEALK+AD+I ++ +G+V+Q TP+++L +P +DFV FVG+ R Sbjct: 182 HQNLKKTIVFVTHDMDEALKLADKICIMKDGKVIQYDTPDKILKNPKDDFVKTFVGENRI 241 Query: 247 --QPALPQGSAHHIMIDGLSIGSESPIS-EALEVMRINRVEGLAVIGKWGDLLGIVGIGE 303 QP L + IMI+ + S S +ALE+M+ N V+ L V K LLGIV + E Sbjct: 242 WNQPELIK--VKDIMINNVIKSSPERTSIQALEIMKSNHVDRLIVASKDNKLLGIVTLRE 299 Query: 304 IGQHLPLDSRLKVGDLLQTDTVALQEHTPLSEVFELMRRSPI--LPVVDDDNRLIGTVTR 361 + + + +LK D+++ + + E+ + V +++ I +PV+++D+ L+G +T+ Sbjct: 300 LKFSINENKKLK--DIMKNNITTINENDSIVNVLDIVENQKIGFIPVINNDSSLVGLITK 357 Query: 362 SCVIEAL-GRHLHREVS 377 S ++ L + +++EV+ Sbjct: 358 SSLLSVLSNQFINKEVN 374 >ref|ZP_00180945.1| hypothetical protein Mther100801 [Moorella thermoacetica ATCC 39073] Length = 376 Score = 348 bits (893), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 183/375 (48%), Positives = 253/375 (67%), Gaps = 6/375 (1%) Query: 9 FRNVSKDY-GSGSVLRNFNLTVPKGQIVTIIGPSGCGKTTLLKMINRLVEPSIGNVLVEG 67 F +VSK Y G+ + +FNL V G+ + +IGPSGCGKTT LKM+NRL+EP+ G + + G Sbjct: 4 FEHVSKVYDGNRIAVADFNLEVEAGEFIVLIGPSGCGKTTTLKMVNRLIEPTSGAIYLNG 63 Query: 68 TDIGTLDAVELRRDIGYVIQQIGLFPHMTIEENISIVPKLKGAGKTERKERVDALLSLIG 127 DI + V LRR IGYVIQQI LFP+MTI +N+ +VP+L G R++RV LL L+G Sbjct: 64 KDIREQNPVALRRHIGYVIQQIALFPNMTIAQNVDVVPRLLGWPAERRRQRVCELLELVG 123 Query: 128 LPPEQYRKRYPHELSGGQQQRIGVARALASDPSIILMDEPFSALDPISRVQLQKELINLN 187 + P+ Y RYP ELSGGQQQRIGV RALA++P +ILMDEPF ALDPI+R LQ+EL L Sbjct: 124 MDPDDYADRYPSELSGGQQQRIGVLRALAAEPPLILMDEPFGALDPITRENLQEELKALQ 183 Query: 188 QRLHKTIVFVTHDIDEALKIADRIILLNEGEVVQNSTPEELLAHPANDFVIEFVGQERFQ 247 +LHKTI+FVTHD+DEALKIADRI+++ +G +VQ + PEELL HPAN+FV F+G+ER Sbjct: 184 AKLHKTILFVTHDMDEALKIADRIVVMKDGYIVQVAAPEELLRHPANEFVASFIGKERLA 243 Query: 248 PALPQGSAHHIMI-DGLSIGSESPISEALEVMRINRVEGLAVIGKWGDLLGIVGIGEIGQ 306 P L + +MI + +++ + ++E + MR +V+ L V + G LLG V I E+ + Sbjct: 244 PGLELRTVEQVMIGEPVTVRPHTGVAEGVATMRRKKVDTLLVTDESGRLLGAVSIEELNR 303 Query: 307 HLPLDSRLKVGDLLQTDTVALQEHTPLSEVFELMRRSPI--LPVVDDDNRLIGTVTRSCV 364 + +V DL+ D + E TP E F+L+ R + LPV+D + RL G VTR+ + Sbjct: 304 N--YQRAHQVQDLMARDVPVVFEGTPAREAFDLITRERLEYLPVIDKEGRLKGLVTRTSM 361 Query: 365 IEALGRHLHREVSSA 379 + AL + + +SA Sbjct: 362 VNALASVVWGDEASA 376 >ref|ZP_00182739.1| hypothetical protein Exigu193501 [Exiguobacterium sp. 255-15] Length = 375 Score = 337 bits (863), Expect = 4e-91, Method: Compositional matrix adjust. Identities = 173/372 (46%), Positives = 246/372 (66%), Gaps = 5/372 (1%) Query: 7 VEFRNVSKDYGSGSVLRNFNLTVPKGQIVTIIGPSGCGKTTLLKMINRLVEPSIGNVLVE 66 +EF+NV K + VL+ L + +G++V IGPSGCGKTT LKMINRL+EPS G +LV Sbjct: 2 IEFKNVGKQFKDNVVLKGLTLEIKEGELVVFIGPSGCGKTTSLKMINRLIEPSSGTILVN 61 Query: 67 GTDIGTLDAVELRRDIGYVIQQIGLFPHMTIEENISIVPKLKGAGKTERKERVDALLSLI 126 G DI D +ELRR +GYVIQQ GLFPHMT+ ENI ++ L+G + R + LL ++ Sbjct: 62 GKDIMKTDTIELRRHMGYVIQQTGLFPHMTVRENIQLIAGLEGKDHDQMDVRTEELLQMV 121 Query: 127 GLPPEQYRKRYPHELSGGQQQRIGVARALASDPSIILMDEPFSALDPISRVQLQKELINL 186 GL P+Q+ RYP ELSGGQQQR+G ARAL +DP +ILMDEPFSALDP++R LQ+EL NL Sbjct: 122 GLDPKQFIDRYPSELSGGQQQRVGFARALMNDPDVILMDEPFSALDPVTRNDLQEELFNL 181 Query: 187 NQRLHKTIVFVTHDIDEALKIADRIILLNEGEVVQNSTPEELLAHPANDFVIEFVGQERF 246 + + KTIVFVTHD+DEA+K+ADRI ++ +GE+VQ TPEE+L HP +++V F+G+ + Sbjct: 182 QEEVKKTIVFVTHDMDEAIKLADRICIMRDGEIVQFDTPEEILRHPKDEYVESFIGKNKI 241 Query: 247 QPALPQGSAHHIMI-DGLSIGSESPISEALEVMRINRVEGLAVIGKWGDLLGIVGIGEIG 305 + A IMI D +SI + + + +E+MR +V+ L + + L GIV + I Sbjct: 242 WSSPAFIKAEDIMIEDPVSISGKRTLLQGIEIMRGRKVDSLLITDRDRILQGIVKLKNI- 300 Query: 306 QHLPLDSRLKVGDLLQTDTVALQEHTPLSEVFELM--RRSPILPVVDDDNRLIGTVTRSC 363 Q P D +++ D+++ + VA+ E L +V E+M + LPV + +L G +TRS Sbjct: 301 QTAP-DKGVRIEDVMEGELVAVDEQDSLLDVLEVMNHEETGYLPVTNKSGQLRGLITRSS 359 Query: 364 VIEALGRHLHRE 375 ++ L +E Sbjct: 360 LLSVLSEQFIQE 371 >ref|NP_781074.1| glycine/betaine transport ATP-binding protein [Clostridium tetani E88] gb|AAO35011.1| glycine/betaine transport ATP-binding protein [Clostridium tetani E88] Length = 368 Score = 336 bits (862), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 170/369 (46%), Positives = 245/369 (66%), Gaps = 9/369 (2%) Query: 7 VEFRNVSKDYGSGSVLRNFNLTVPKGQIVTIIGPSGCGKTTLLKMINRLVEPSIGNVLVE 66 +EF NVSK + + SVL N +V KG++V +IGPSGCGKTT LKMIN+L+ PS G + + Sbjct: 2 IEFTNVSKSFKNTSVLENVTFSVKKGELVVLIGPSGCGKTTTLKMINKLILPSSGKITIS 61 Query: 67 GTDIGTLDAVELRRDIGYVIQQIGLFPHMTIEENISIVPKLKGAGKTERKERVDALLSLI 126 G D+ D ++LRR+IGYVIQQ GLFPHMT+ ENI ++P L+ + R +R LL L+ Sbjct: 62 GEDLSQKDTIQLRRNIGYVIQQTGLFPHMTVGENIDLIPYLQKWDEKTRHKRTQELLELV 121 Query: 127 GLPPEQYRKRYPHELSGGQQQRIGVARALASDPSIILMDEPFSALDPISRVQLQKELINL 186 GL P++Y RYP ELSGGQQQR+GVARALA +P IILMDEPFSALDPI+R LQ E+ L Sbjct: 122 GLDPDKYIDRYPEELSGGQQQRVGVARALAVNPDIILMDEPFSALDPITRNSLQDEVFKL 181 Query: 187 NQRLHKTIVFVTHDIDEALKIADRIILLNEGEVVQNSTPEELLAHPANDFVIEFVGQERF 246 Q+L KTIVFVTHD+DEALK+ D++ ++ +G ++Q P+ +L +PAN+FV F+G+ R Sbjct: 182 QQKLQKTIVFVTHDMDEALKLGDKVCIMKDGHIMQFDEPQTILRNPANEFVANFIGKNRL 241 Query: 247 QPALPQGSAHHIMIDG-LSIGSESPISEALEVMRINRVEGLAVIGKWGDLLGIVGIGEIG 305 +A IMI + E I +++ +M+ N V+ + V+ K L GIV + Sbjct: 242 WSNPELITAEDIMIKNPIKTFGERTIVQSVNIMKQNHVDSILVVDKDNILKGIVTL---- 297 Query: 306 QHLPLDSR--LKVGDLLQTDTVALQEHTPLSEVFELMRRSPI--LPVVDDDNRLIGTVTR 361 +HL LD+ K+ D++ +D ++ + +V ++M+ + I +PVVD D +L G +T+ Sbjct: 298 KHLDLDNVNGKKLKDVMASDLKYVELEDSIMDVIKVMKENSISYIPVVDKDKKLKGLITQ 357 Query: 362 SCVIEALGR 370 S +I + Sbjct: 358 SSLITTFSK 366 >ref|NP_391253.1| choline ABC transporter (ATP-binding protein) [Bacillus subtilis] sp|Q45460|OPBA_BACSU Choline transport ATP-binding protein opuBA pir||G69669 choline ABC transporter (ATP-binding protein) opuBA - Bacillus subtilis gb|AAC14356.1| ATPase [Bacillus subtilis] emb|CAB15378.1| choline ABC transporter (ATP-binding protein) [Bacillus subtilis subsp. subtilis str. 168] Length = 381 Score = 334 bits (857), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 188/376 (50%), Positives = 248/376 (65%), Gaps = 15/376 (3%) Query: 9 FRNVSKDYGSGS-VLRNFNLTVPKGQIVTIIGPSGCGKTTLLKMINRLVEPSIGNVLVEG 67 NVSK Y G + N NL + KG+ + IGPSGCGKTT +KMINRL+EPS G + ++G Sbjct: 4 LENVSKTYKGGKKAVNNVNLKIAKGEFICFIGPSGCGKTTTMKMINRLIEPSAGKIFIDG 63 Query: 68 TDIGTLDAVELRRDIGYVIQQIGLFPHMTIEENISIVPKLKGAGKTERKERVDALLSLIG 127 +I D VELRR IGYVIQQIGLFPHMTI++NIS+VPKL + +RKER LL L+ Sbjct: 64 ENIMDQDPVELRRKIGYVIQQIGLFPHMTIQQNISLVPKLLKWPEQQRKERARELLKLVD 123 Query: 128 LPPEQYRKRYPHELSGGQQQRIGVARALASDPSIILMDEPFSALDPISRVQLQKELINLN 187 + PE Y RYPHELSGGQQQRIGV RALA++P +ILMDEPF ALDPI+R LQ+E L Sbjct: 124 MGPE-YVDRYPHELSGGQQQRIGVLRALAAEPPLILMDEPFGALDPITRDSLQEEFKKLQ 182 Query: 188 QRLHKTIVFVTHDIDEALKIADRIILLNEGEVVQNSTPEELLAHPANDFVIEFVGQERF- 246 + LHKTIVFVTHD+DEA+K+ADRI++L GE+VQ TP+++L +PA++FV EF+G+ER Sbjct: 183 KTLHKTIVFVTHDMDEAIKLADRIVILKAGEIVQVGTPDDILRNPADEFVEEFIGKERLI 242 Query: 247 QPALPQ-GSAHHIM-IDGLSIGSESPISEALEVMRINRVEGLAVIGKWGDLLGIVGIGEI 304 Q + P IM ++I ++ +SEA+++MR RV+ L V+ L G V + I Sbjct: 243 QSSSPDVERVDQIMNTQPVTITADKTLSEAIQLMRQERVDSLLVVNDERVLQGYVDVEII 302 Query: 305 GQHLPLDSRLK---VGDLLQTDTVALQEHTPLSEVFE--LMRRSPILPVVDDDNRLIGTV 359 Q R K V ++L D + T L + L R +PVVD+D RLIG V Sbjct: 303 DQ-----CRKKANLVSEVLHEDIYTVLGGTLLRDTVRKILKRGVKYVPVVDEDRRLIGIV 357 Query: 360 TRSCVIEALGRHLHRE 375 TR+ +++ + L E Sbjct: 358 TRASLVDIVYDSLWGE 373 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.139 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 538,482,164 Number of extensions: 22625621 Number of successful extensions: 158000 Number of sequences better than 1.0e-05: 23265 Number of HSP's gapped: 122895 Number of HSP's successfully gapped: 25077 Length of query: 379 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 252 Effective length of database: 412,420,005 Effective search space: 103929841260 Effective search space used: 103929841260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)