BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0520.0025.p (274 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00188348.1| hypothetical protein Rxyl041301 [Rubrobacter ... 226 3e-58 ref|NP_898784.1| conserved hypothetical protein [Rhodococcus ery... 182 9e-45 ref|NP_107683.1| hypothetical protein mll7342 [Mesorhizobium lot... 179 6e-44 ref|NP_103660.1| hypothetical protein mll2277 [Mesorhizobium lot... 171 2e-41 gb|EAJ42133.1| unknown [environmental sequence] 169 4e-41 >ref|ZP_00188348.1| hypothetical protein Rxyl041301 [Rubrobacter xylanophilus DSM 9941] Length = 274 Score = 226 bits (577), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 114/240 (47%), Positives = 154/240 (64%), Gaps = 3/240 (1%) Query: 26 KKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTASDRKSFFPLKGEASYW 85 ++V V GE +A+S + LLHE G LPVYYFP DVR +LL +D + P KGEA YW Sbjct: 36 RRVRVELGGETVADSRRMKLLHETGLLPVYYFPEEDVRTELLERTDHTTRCPFKGEAVYW 95 Query: 86 SLQLGDEISENAAWSYEHPIPGREDIAGYYAFYWNAVDGWXXXXXXXXXXXXXDPYKRID 145 +++ G +ENAAW+Y P+ G + G+ AFYW+ +D W DPY RID Sbjct: 96 TVRAGGRTAENAAWAYPEPLEGAPPLGGHIAFYWDRMDRWFEEDEEVDVHPR-DPYHRID 154 Query: 146 ALRSSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLPEDDLNTALLEFSDSKTACPYKG 205 AL SSRH+ + +NG +A++ RPVILFETG+P RYY+P +D+ LL S+S + CPYKG Sbjct: 155 ALPSSRHVRVTVNGELVAETRRPVILFETGLPPRYYIPREDVREELLVPSESSSVCPYKG 214 Query: 206 HASYRSVRIHDELHKDLVWTYASPIAEIPQIAGLFSFYNERVEIFVDGKRQ--PKQAWYR 263 ASY SVR E +DLVW+Y P + ++ GL F+NERV++ VDG+RQ P+ W R Sbjct: 215 VASYWSVRAGGETVEDLVWSYPEPRRDAERVGGLLCFFNERVDLEVDGERQERPETQWSR 274 Score = 80.5 bits (197), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 39/100 (39%), Positives = 57/100 (57%) Query: 149 SSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLPEDDLNTALLEFSDSKTACPYKGHAS 208 S R + + L G +ADS R +L ETG+ YY PE+D+ T LLE +D T CP+KG A Sbjct: 34 SPRRVRVELGGETVADSRRMKLLHETGLLPVYYFPEEDVRTELLERTDHTTRCPFKGEAV 93 Query: 209 YRSVRIHDELHKDLVWTYASPIAEIPQIAGLFSFYNERVE 248 Y +VR ++ W Y P+ P + G +FY +R++ Sbjct: 94 YWTVRAGGRTAENAAWAYPEPLEGAPPLGGHIAFYWDRMD 133 Score = 76.3 bits (186), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 40/99 (40%), Positives = 54/99 (54%) Query: 25 AKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTASDRKSFFPLKGEASY 84 ++ V V GE++AE+ ++L E G P YY P DVR +LL S+ S P KG ASY Sbjct: 159 SRHVRVTVNGELVAETRRPVILFETGLPPRYYIPREDVREELLVPSESSSVCPYKGVASY 218 Query: 85 WSLQLGDEISENAAWSYEHPIPGREDIAGYYAFYWNAVD 123 WS++ G E E+ WSY P E + G F+ VD Sbjct: 219 WSVRAGGETVEDLVWSYPEPRRDAERVGGLLCFFNERVD 257 >ref|NP_898784.1| conserved hypothetical protein [Rhodococcus erythropolis] gb|AAP74054.1| conserved hypothetical protein [Rhodococcus erythropolis] Length = 265 Score = 182 bits (461), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 96/245 (39%), Positives = 137/245 (55%), Gaps = 3/245 (1%) Query: 11 EQRPQKLNVKTDRIAKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTAS 70 +Q + + +R K++ V + G +IA++T + L E+G LPVYY P DVR D L Sbjct: 12 QQSGENGRISLERNHKRIRVFFAGHVIADTTRAVYLFEKGHLPVYYIPRDDVRTDSLEPL 71 Query: 71 DRKSFFPLKGEASYWSLQLGDEISENAAWSYEHPIPGREDIAGYYAFYWNAVDGWXXXXX 130 D ++ P KG+A YW L + D +E + W Y+ P+ D++ Y AFYW+ +D W Sbjct: 72 DATTWCPWKGKARYWDLVVDDRRAERSLWGYDEPLKDSLDLSWYVAFYWHKMDAW-YEED 130 Query: 131 XXXXXXXXDPYKRIDALRSSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLPEDDLNTA 190 DPY R+D L SSRH+E+++ +AD+T P +LFET +PVRYY+P D+ + Sbjct: 131 QQVFVHPRDPYVRVDVLPSSRHVEVFIGDTLVADTTHPRLLFETSLPVRYYIPRIDVRSE 190 Query: 191 LLEFSDSKTACPYKGHASYRSVRIHDELH--KDLVWTYASPIAEIPQIAGLFSFYNERVE 248 SD+KT+CPYKG ASY S D+ +D W Y AE I SFY + Sbjct: 191 FFTGSDAKTSCPYKGTASYLSFTGSDDSDPVEDAAWYYPFTTAEASGIGDHVSFYPDGAR 250 Query: 249 IFVDG 253 I VDG Sbjct: 251 IVVDG 255 Score = 68.6 bits (166), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 37/114 (32%), Positives = 62/114 (54%) Query: 143 RIDALRSSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLPEDDLNTALLEFSDSKTACP 202 RI R+ + I ++ G +AD+TR V LFE G YY+P DD+ T LE D+ T CP Sbjct: 19 RISLERNHKRIRVFFAGHVIADTTRAVYLFEKGHLPVYYIPRDDVRTDSLEPLDATTWCP 78 Query: 203 YKGHASYRSVRIHDELHKDLVWTYASPIAEIPQIAGLFSFYNERVEIFVDGKRQ 256 +KG A Y + + D + +W Y P+ + ++ +FY +++ + + +Q Sbjct: 79 WKGKARYWDLVVDDRRAERSLWGYDEPLKDSLDLSWYVAFYWHKMDAWYEEDQQ 132 >ref|NP_107683.1| hypothetical protein mll7342 [Mesorhizobium loti MAFF303099] dbj|BAB53469.1| hypothetical protein [Mesorhizobium loti] Length = 247 Score = 179 bits (454), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 95/238 (39%), Positives = 139/238 (58%), Gaps = 2/238 (0%) Query: 24 IAKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTASDRKSFFPLKGEAS 83 + K++ V+ I+A++T L+L+E LPVYYFP DVR + L S + P KG A+ Sbjct: 3 MQKRLRVQVGSMIVADTTKALVLYESDHLPVYYFPMSDVREEFLLPSRTTTESPFKGVAT 62 Query: 84 YWSLQLGDEISENAAWSYEHPIPGREDIAGYYAFYWNAVDGWXXXXXXXXXXXXXDPYKR 143 ++SL G + E+AAW Y P+ G D++GY +FYW + W DP++R Sbjct: 63 HYSLNTGITLVEDAAWRYLDPVKGSPDLSGYMSFYWPKMTHWYEEDEEIFVHAR-DPFRR 121 Query: 144 IDALRSSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLPEDDLNTALLEFSDSKTACPY 203 +D L SSR +++ L+G ++ADS R V LFETG PVR+YLP D + S + CPY Sbjct: 122 VDCLPSSRRVQVILDGEQVADSRRGVFLFETGHPVRHYLPISDTRLDMFAPSRYISRCPY 181 Query: 204 KGHASYRSVRIHDELHKDLVWTYASPIAEIPQIAGLFSFYNERVE-IFVDGKRQPKQA 260 KG ++Y V + H++LVW Y P+ E +I GL F++E V+ I VDG K+A Sbjct: 182 KGISNYYHVTTPQKRHENLVWYYPEPVHEAERIKGLVCFHHELVDKILVDGVDISKEA 239 >ref|NP_103660.1| hypothetical protein mll2277 [Mesorhizobium loti MAFF303099] dbj|BAB49446.1| hypothetical protein [Mesorhizobium loti] Length = 257 Score = 171 bits (432), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 100/253 (39%), Positives = 140/253 (55%), Gaps = 6/253 (2%) Query: 5 ELQEVREQRPQKLNVKTDRIAKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRN 64 LQ P+ +NV K+V V++ G IA+S +VL+L LP+Y+FP V Sbjct: 6 NLQPREATAPRLVNVLHS--PKRVRVKFGGRTIADSRNVLVLRSNHFLPIYFFPPSSVDA 63 Query: 65 DLLTASDRKSFFPLKGEASYWSLQLGDEISENAAWSYEHPI-PGREDIAGYYAFYWNAVD 123 +L S + + GE +YW + GD + NAAWS+ P +AG AF W AVD Sbjct: 64 SVLKPSLHREPHAVGGETAYWDIDSGDRRAANAAWSFTAPPDENLSPLAGRIAFTWKAVD 123 Query: 124 GWXXXXXXXXXXXXXDPYKRIDALRSSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLP 183 W DPY RID L SS H++++ +G +ADS RPV+LFET +P R+YL Sbjct: 124 QWFEEEEEVFVHAR-DPYARIDVLHSSSHVQVWFDGDIIADSHRPVLLFETHLPTRFYLR 182 Query: 184 EDDLNTALLEFSDSKTACPYKGHASYRSVRIHD-ELHKDLVWTYASPIAEIPQIAGLFSF 242 +D+ L S S T CPYKG ASY S + D + D+ W+Y PI E+P+I GL +F Sbjct: 183 PEDVRLERLTASSSTTRCPYKGIASYWSGLLKDGSVRPDIAWSYRDPIDEMPKIKGLIAF 242 Query: 243 YNERVE-IFVDGK 254 Y + V+ I +DG+ Sbjct: 243 YPQAVDRIHLDGQ 255 >gb|EAJ42133.1| unknown [environmental sequence] Length = 250 Score = 169 bits (429), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 87/236 (36%), Positives = 134/236 (56%), Gaps = 3/236 (1%) Query: 18 NVKTDRIAKKVSVRWRGEIIAESTDVLLLHERGQLPVYYFPAGDVRNDLLTASDRKSFFP 77 N+ ++ K V R E+I +S +L E+G LP+YYFP +R DLL + + +F P Sbjct: 17 NIILEKSEKWVRGRINNEMIIDSKKTFILLEKGHLPIYYFPKESIRMDLLIKNTKTTFCP 76 Query: 78 LKGEASYWSLQLGDEISENAAWSYEHPIPGREDIAGYYAFYWNAVDGWXXXXXXXXXXXX 137 LKGEA+YW +E + AW YE+PI + ++ G AFYWN +D W Sbjct: 77 LKGEATYWDFNYKNENILDIAWCYENPIQQQRELRGLVAFYWNKLDNW-YEEAEEIYSYP 135 Query: 138 XDPYKRIDALRSSRHIEIYLNGVKLADSTRPVILFETGVPVRYYLPEDDLNTALLEFSDS 197 DPY R+DAL+SSR+++I+L+ + + R VILFET +Y+ P +D+ L+ FS+ Sbjct: 136 RDPYVRVDALKSSRNVKIFLDDNLILTTNRSVILFETHKKPKYFFPINDIKVNLV-FSEK 194 Query: 198 KTACPYKGHASYRSVRIHDELHKDLVWTYASPIAEIPQIAGLFSFYNER-VEIFVD 252 CPYKG ++Y S++ +++ KD+V Y P E+ + L F N + I +D Sbjct: 195 IFRCPYKGISNYLSIKDNNKTFKDIVMQYTEPRPEVSILKNLVCFENNNLLSIIID 250 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 404,817,937 Number of extensions: 16386025 Number of successful extensions: 30579 Number of sequences better than 1.0e-05: 66 Number of HSP's gapped: 30548 Number of HSP's successfully gapped: 114 Length of query: 274 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 150 Effective length of database: 420,565,302 Effective search space: 63084795300 Effective search space used: 63084795300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)