BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0518.0003.p (401 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00181462.1| hypothetical protein Mther054601 [Moorella th... 461 e-128 emb|CAB15129.2| alanine transaminase [Bacillus subtilis subsp. s... 444 e-123 ref|NP_244216.1| aspartate aminotransferase [Bacillus halodurans... 436 e-121 ref|ZP_00061526.1| COG0436: Aspartate/tyrosine/aromatic aminotra... 431 e-119 ref|ZP_00078609.1| COG0436: Aspartate/tyrosine/aromatic aminotra... 419 e-116 >ref|ZP_00181462.1| hypothetical protein Mther054601 [Moorella thermoacetica ATCC 39073] Length = 404 Score = 461 bits (1185), Expect = e-128, Method: Compositional matrix adjust. Identities = 224/382 (58%), Positives = 280/382 (73%) Query: 20 IAPRVREIAPSGIRAFFDLSAGNNEIISLGVGEPDFTTPEHIRAACIRALEQGETMYTPN 79 I P VR++ PSGIR FF+L A +ISLGVGEPDF TP HIR AC+++LE+G TMYT N Sbjct: 13 ITPVVRDLPPSGIRRFFELVASTKGVISLGVGEPDFVTPWHIREACVQSLERGYTMYTSN 72 Query: 80 AGLPELREEIARYQQESFGLAYDPRDEVVVTVGSSEALDLALRAFTAPGDEVIIPSPSYI 139 GLPELR IA Y FGL YDP +++VT+G+SEA+DLALR PGDEV+IP P Y+ Sbjct: 73 YGLPELRRAIADYLAWRFGLTYDPMKQIMVTIGASEAVDLALRTVLNPGDEVLIPEPCYV 132 Query: 140 AYSPIAYLNGGTLVEVEAAAEQGFKLTAEALQKAITPRSKLLMVNFPNNPTGAVMSYEDW 199 +Y PI L GG V + GF LTA L+ ITPRSK+L++ FPNNPTGAV+S E+ Sbjct: 133 SYQPITQLAGGIPVPIPTTMTDGFALTAARLEHYITPRSKVLILCFPNNPTGAVLSREEM 192 Query: 200 MPIAEIVKAHNLIVLSDEIYAELTYDSRHVSIASLPGMKERTIVISGFSKAFAMTGWRVG 259 IA +V+ +NL+V+SDEIYAEL Y+ + +S ASLPGM+ERTI++SGFSKAFAMTGWR+G Sbjct: 193 QAIARLVEKYNLLVISDEIYAELRYEGQPLSFASLPGMQERTILVSGFSKAFAMTGWRIG 252 Query: 260 YACGNRELLAAMLKIHQYTAMCAPVLGQIAAVESLRHGLPDKDYMKECFRQRRSLFVEGL 319 Y + + LAAM+KIHQYT +CAPV+GQ+AA+E+LRHG D + M E + QRR L L Sbjct: 253 YVAAHPDFLAAMVKIHQYTILCAPVMGQMAALEALRHGRQDVERMVEQYDQRRRLVYSRL 312 Query: 320 RSIGLHCHEPQGAFYAFPSIRHTGMKSEDFALRLLREAGVAVVPGHVFGTGGEGHLRCSY 379 R +GL C EP+GAFY FPSI TG+ S FA LL+E VAVVPG FG GEG +RCSY Sbjct: 313 REMGLDCFEPRGAFYIFPSIAATGLDSVTFAEELLKEEKVAVVPGTAFGASGEGFIRCSY 372 Query: 380 AASPAKLTEALERLEGFMKVKM 401 AAS A LTEA+ R+E F+ ++ Sbjct: 373 AASLADLTEAMNRMERFVARRL 394 >emb|CAB15129.2| alanine transaminase [Bacillus subtilis subsp. subtilis str. 168] Length = 386 Score = 444 bits (1141), Expect = e-123, Method: Compositional matrix adjust. Identities = 215/382 (56%), Positives = 276/382 (72%) Query: 16 LSERIAPRVREIAPSGIRAFFDLSAGNNEIISLGVGEPDFTTPEHIRAACIRALEQGETM 75 ++ ++ V++I PSGIR FFDL+A +ISLGVGEPDF T ++R A I +LEQG T Sbjct: 1 MTSYLSDYVQQIKPSGIRKFFDLAATMEGVISLGVGEPDFVTAWNVREASILSLEQGYTS 60 Query: 76 YTPNAGLPELREEIARYQQESFGLAYDPRDEVVVTVGSSEALDLALRAFTAPGDEVIIPS 135 YT NAGL LREEI+RY F L+Y P +E++VTVG+S+ALD+A+RA PG+EVIIP Sbjct: 61 YTANAGLYSLREEISRYLSNRFDLSYSPDNELIVTVGASQALDIAIRAIVNPGEEVIIPE 120 Query: 136 PSYIAYSPIAYLNGGTLVEVEAAAEQGFKLTAEALQKAITPRSKLLMVNFPNNPTGAVMS 195 P ++AY + L GG V V A++GFK TA + A+T ++K +++ P+NPTG+V S Sbjct: 121 PCFVAYDALVSLAGGIPVHVHTTADKGFKATAADFEAAVTEKTKAILICSPSNPTGSVYS 180 Query: 196 YEDWMPIAEIVKAHNLIVLSDEIYAELTYDSRHVSIASLPGMKERTIVISGFSKAFAMTG 255 E+ IAE K H++IVL+DEIYAELTYD SIA+LPGMKERT+VISGFSKAFAMTG Sbjct: 181 KEELNEIAEFAKKHDVIVLADEIYAELTYDEEFTSIAALPGMKERTVVISGFSKAFAMTG 240 Query: 256 WRVGYACGNRELLAAMLKIHQYTAMCAPVLGQIAAVESLRHGLPDKDYMKECFRQRRSLF 315 WR+G+A L AMLKIHQY MCAP + Q AA+E L++G+ D + MK+ +R+RR+LF Sbjct: 241 WRLGFAAAPSLLRDAMLKIHQYAMMCAPAMAQFAALEGLKNGMEDVEKMKKSYRRRRNLF 300 Query: 316 VEGLRSIGLHCHEPQGAFYAFPSIRHTGMKSEDFALRLLREAGVAVVPGHVFGTGGEGHL 375 VE L IGL CH P GAFYAFPSI+ GM SE FA LL + VAVVPG VFG GEG++ Sbjct: 301 VESLNEIGLSCHHPGGAFYAFPSIKSMGMSSEQFAEELLTQEKVAVVPGSVFGPSGEGYI 360 Query: 376 RCSYAASPAKLTEALERLEGFM 397 RCSYA S +L EAL R++ F+ Sbjct: 361 RCSYATSIEQLQEALVRMKRFL 382 >ref|NP_244216.1| aspartate aminotransferase [Bacillus halodurans] pir||F84068 aspartate aminotransferase BH3350 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07069.1| aspartate aminotransferase [Bacillus halodurans] Length = 393 Score = 436 bits (1121), Expect = e-121, Method: Compositional matrix adjust. Identities = 205/380 (53%), Positives = 277/380 (72%) Query: 19 RIAPRVREIAPSGIRAFFDLSAGNNEIISLGVGEPDFTTPEHIRAACIRALEQGETMYTP 78 R++ RV+ I PSGIR FFDL++ IISLGVGEPDF TP ++R A I ++E+G T Y+ Sbjct: 10 RLSTRVQSIQPSGIRRFFDLASKMENIISLGVGEPDFVTPWNVREASISSMERGFTAYSA 69 Query: 79 NAGLPELREEIARYQQESFGLAYDPRDEVVVTVGSSEALDLALRAFTAPGDEVIIPSPSY 138 NAG+ ELRE I+RY E F + YDP E++VTVG+SEA+D+ +RA GDEVI+ PS+ Sbjct: 70 NAGIIELREAISRYLYERFHIGYDPESEILVTVGASEAIDIGMRAIIDEGDEVIVVEPSF 129 Query: 139 IAYSPIAYLNGGTLVEVEAAAEQGFKLTAEALQKAITPRSKLLMVNFPNNPTGAVMSYED 198 ++Y+P+ + GG V V + F++T ++ AITPR+K +++ FPNNPTG++M E+ Sbjct: 130 VSYAPLVTMAGGVPVPVGTHIDTDFQVTPAQIEAAITPRTKAIILCFPNNPTGSIMGKEE 189 Query: 199 WMPIAEIVKAHNLIVLSDEIYAELTYDSRHVSIASLPGMKERTIVISGFSKAFAMTGWRV 258 +A+++ ++LIV SDEIYAELTYD HVS+AS+ GM+ERT++ISGFSKAFAMTGWR+ Sbjct: 190 LEAVAKVISGNDLIVFSDEIYAELTYDGTHVSLASMDGMRERTVLISGFSKAFAMTGWRL 249 Query: 259 GYACGNRELLAAMLKIHQYTAMCAPVLGQIAAVESLRHGLPDKDYMKECFRQRRSLFVEG 318 GY C ++L+AMLKIHQY+ MCAP + Q A+E+L G+ D M + +RQRR+ V+ Sbjct: 250 GYVCAPDDILSAMLKIHQYSLMCAPTMAQHGALEALETGMDDVHRMVQSYRQRRNFVVKT 309 Query: 319 LRSIGLHCHEPQGAFYAFPSIRHTGMKSEDFALRLLREAGVAVVPGHVFGTGGEGHLRCS 378 IGL C P GAFYAFPS++ TG+ SE+FA RLL E VAVVPG+VFG GGEGH+RCS Sbjct: 310 FTEIGLTCPMPGGAFYAFPSVKETGLTSEEFAERLLMEEHVAVVPGNVFGEGGEGHIRCS 369 Query: 379 YAASPAKLTEALERLEGFMK 398 YA S L ALER+ F++ Sbjct: 370 YATSMEHLETALERIGRFVQ 389 >ref|ZP_00061526.1| COG0436: Aspartate/tyrosine/aromatic aminotransferase [Clostridium thermocellum ATCC 27405] Length = 390 Score = 431 bits (1109), Expect = e-119, Method: Compositional matrix adjust. Identities = 205/383 (53%), Positives = 272/383 (71%) Query: 16 LSERIAPRVREIAPSGIRAFFDLSAGNNEIISLGVGEPDFTTPEHIRAACIRALEQGETM 75 ++E + P VR++ PSGIR FFDL + ISL +GEPDF TP +IR A I +LE+G T Sbjct: 3 IAEMVLPTVRQVPPSGIRKFFDLVNEMKDAISLSIGEPDFVTPWNIREAGIYSLEKGHTH 62 Query: 76 YTPNAGLPELREEIARYQQESFGLAYDPRDEVVVTVGSSEALDLALRAFTAPGDEVIIPS 135 Y+PNAG ELREEI +YQ+ + L YDP+ +V+VTVG SE +D+ALRA PGDEVIIP Sbjct: 63 YSPNAGFMELREEICKYQERKYNLKYDPKTQVIVTVGGSEGIDIALRALVGPGDEVIIPE 122 Query: 136 PSYIAYSPIAYLNGGTLVEVEAAAEQGFKLTAEALQKAITPRSKLLMVNFPNNPTGAVMS 195 P ++AY P G T V V +AE F+LT E L+KAIT R+K+L++ +PNNPTGAVM Sbjct: 123 PCFVAYKPCTIFTGATPVIVNLSAENEFRLTPELLEKAITDRTKVLILPYPNNPTGAVME 182 Query: 196 YEDWMPIAEIVKAHNLIVLSDEIYAELTYDSRHVSIASLPGMKERTIVISGFSKAFAMTG 255 +D I E++K ++IV+SDEIY+ELTY +HVSIAS P MK++T+VI+GFSKA+AMTG Sbjct: 183 KKDLEAIVEVLKDKDIIVISDEIYSELTYGVKHVSIASFPEMKDKTVVINGFSKAYAMTG 242 Query: 256 WRVGYACGNRELLAAMLKIHQYTAMCAPVLGQIAAVESLRHGLPDKDYMKECFRQRRSLF 315 WR+GY G+ +L++ M KIHQY MC+P Q AA+E+LR+ D + M + +RR + Sbjct: 243 WRLGYCLGHPDLISQMYKIHQYAIMCSPTTAQYAAIEALRNSDDDVEAMVREYNRRRRIM 302 Query: 316 VEGLRSIGLHCHEPQGAFYAFPSIRHTGMKSEDFALRLLREAGVAVVPGHVFGTGGEGHL 375 VEG RS GL C EP GAFY FP I TGM S +F+ RLL E VAVVPG FG GEG++ Sbjct: 303 VEGFRSAGLECFEPLGAFYVFPCIESTGMTSYEFSERLLMEEKVAVVPGTAFGECGEGYV 362 Query: 376 RCSYAASPAKLTEALERLEGFMK 398 R YA+S + EA++R++ F++ Sbjct: 363 RSCYASSMDNIIEAMKRIKRFVE 385 >ref|ZP_00078609.1| COG0436: Aspartate/tyrosine/aromatic aminotransferase [Methanosarcina barkeri] Length = 396 Score = 419 bits (1077), Expect = e-116, Method: Compositional matrix adjust. Identities = 201/381 (52%), Positives = 270/381 (70%) Query: 17 SERIAPRVREIAPSGIRAFFDLSAGNNEIISLGVGEPDFTTPEHIRAACIRALEQGETMY 76 S+ +A V+EI PSGIR FFDL +G ++ISLGVGEPDF TP HIR CI +LE+G+T Y Sbjct: 11 SKFVADVVKEIPPSGIRRFFDLVSGLEDVISLGVGEPDFITPWHIREMCIHSLEKGQTSY 70 Query: 77 TPNAGLPELREEIARYQQESFGLAYDPRDEVVVTVGSSEALDLALRAFTAPGDEVIIPSP 136 T N GLPELR+E+ R +GL YDP E+++T G SEALD+A+R PGDEVII P Sbjct: 71 TSNYGLPELRDELTRTYYRRYGLDYDPSSEILITTGVSEALDIAVRTVVNPGDEVIIVQP 130 Query: 137 SYIAYSPIAYLNGGTLVEVEAAAEQGFKLTAEALQKAITPRSKLLMVNFPNNPTGAVMSY 196 SY+AY P L GG V V + F LTAEAL+ AIT ++K +++NFPNNPTGA+MS Sbjct: 131 SYVAYLPSVVLAGGKPVIVSTHRDDEFSLTAEALKPAITDKTKAIILNFPNNPTGAIMSK 190 Query: 197 EDWMPIAEIVKAHNLIVLSDEIYAELTYDSRHVSIASLPGMKERTIVISGFSKAFAMTGW 256 E IA++V ++L V+SDE+Y LTY+ +HV ++SL GMKERT++++GFSKA+AMTG Sbjct: 191 EGLEDIADLVVENDLFVISDEVYECLTYEGKHVPLSSLDGMKERTVMLNGFSKAYAMTGL 250 Query: 257 RVGYACGNRELLAAMLKIHQYTAMCAPVLGQIAAVESLRHGLPDKDYMKECFRQRRSLFV 316 R+G+A G+ E++ +M+ IHQY+ +CAP+ QI A+E+LRHG + + M + +RR V Sbjct: 251 RLGFAMGSPEIIHSMMMIHQYSMLCAPITAQIGAIEALRHGKDEMERMVREYDRRRHFIV 310 Query: 317 EGLRSIGLHCHEPQGAFYAFPSIRHTGMKSEDFALRLLREAGVAVVPGHVFGTGGEGHLR 376 G IGL C P+GAFYAFP + +TG+ S +FA RLL E V +PG FG GEG LR Sbjct: 311 RGFNRIGLECCNPKGAFYAFPYVGNTGLSSSEFAERLLDEKKVVAIPGDAFGEAGEGFLR 370 Query: 377 CSYAASPAKLTEALERLEGFM 397 C+YAAS + +AL+R+E F+ Sbjct: 371 CAYAASINDIRKALDRMEDFV 391 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 594,299,527 Number of extensions: 24650597 Number of successful extensions: 71341 Number of sequences better than 1.0e-05: 3675 Number of HSP's gapped: 64173 Number of HSP's successfully gapped: 3675 Length of query: 401 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 273 Effective length of database: 409,704,906 Effective search space: 111849439338 Effective search space used: 111849439338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)