BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0517.0002.p (1216 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_870899.1| conserved hypothetical protein-putative eukaryo... 118 1e-24 ref|NP_822582.1| hypothetical protein [Streptomyces avermitilis ... 92 8e-17 ref|NP_733499.1| putative secreted protein [Streptomyces coelico... 87 4e-15 ref|NP_631229.1| putative secreted sugar hydrolase [Streptomyces... 78 1e-12 ref|NP_391527.1| ywoF [Bacillus subtilis] >gi|7475519|pir||A7006... 70 3e-10 >ref|NP_870899.1| conserved hypothetical protein-putative eukaryotic thiol (cysteine) proteases [Pirellula sp. 1] emb|CAD77977.1| conserved hypothetical protein-putative eukaryotic thiol (cysteine) proteases [Pirellula sp.] Length = 851 Score = 118 bits (295), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 158/675 (23%), Positives = 251/675 (37%), Gaps = 151/675 (22%) Query: 2 KKRTFIVLSIIALIVGCFPAAAIQAEESHSSEAVYYVSENGSDSNSGT--------KDKP 53 K +F +I L + AE S A +YVS G D+ SGT D P Sbjct: 118 KLNSFRFCLLILLAISMTEGDCCAAEPS----ADFYVSLEGRDNWSGTLAEPNDSQSDGP 173 Query: 54 FLTLEKARDAIRQLKQGAGLPDGGVTVYLRGGTYNRTGSFLLEEQDSGTADKPVTYKAYP 113 F TL +ARDAIR + G V V +RGGTY + +DSG VTY AYP Sbjct: 174 FATLTRARDAIRSSGENRA---GDVLVLIRGGTYRLAKTVDFGLEDSGHPGSTVTYAAYP 230 Query: 114 GESVRLSGGRQLQKGWFTPVTDPSVLNRIISTDARSKVLQADLKGHGITDYGVMSRHGYY 173 GE+ S G+ + W T P L A KV A++ G Sbjct: 231 GETPVFSSGQAITD-WKPVTTAPPGL----PEKAFDKVKVANVSGR-------------- 271 Query: 174 KANDVSQTPPMELYIEGEGMTLARWPNKGTVQMGNIIDPGPTRKDADLQTRGGTFTYTYE 233 + EGM L R ++G + + G +R + + Sbjct: 272 ----------FHTLFDAEGM-LPRARSRGFIPL-----KGGSRNE---------LHFPAG 306 Query: 234 RPDLWTQADDIWLDGIFGYSWEWSYNKIASIDTQNK----SITLAYGEMSGLFKNWYPDF 289 R W +DI + ++W + + S+D Q + SI Y M+ L + Sbjct: 307 RLKNWPNVEDIEIVVRPHHAWIVNILPLKSVDEQKQIARTSIDATYA-MNPLHFLKTTNS 365 Query: 290 HFAQNLLEEIDMPGEYYIDRSKGKLYFMPTAAFQAANP----------EITVSMLKTPMI 339 + +N LEE+D PGE+ ++ GKLY P P +I + + Sbjct: 366 CWVENALEELDEPGEWALNTQTGKLYLWPRNDSPILAPTLKELIRIEGKIDKEGPRDIPV 425 Query: 340 STLNTSFVTF----------------EDLILENGRDSGAVIMGGDSVRLVHCEIRNFTEG 383 + L +TF D + + ++ + G + + C + Sbjct: 426 TNLCLRGLTFTHGERFSIAPDDAGLQHDWDMHDKANALVRLRGTERCTIQQCHFAHSGGS 485 Query: 384 GVRINTQSRWLYNDFAKGTGVNHAIVSTHIHHVGGTGVILNG-GDRLTLAPGNNVVENSH 442 +R++ R+ + I S I H+GG GV+L G G G NV+ N+H Sbjct: 486 AIRVDLHGRY------------NVIDSNVIEHMGGAGVLLCGYGPGTKDVNGGNVIFNNH 533 Query: 443 IHDFAYYHKAYNPAVIL-TGAGNRISHNEIHDAPHPGILIFG------------------ 483 IH H +++P ++L + NR+++N +H+ P+ GI++ G Sbjct: 534 IHHTGRIH-SHSPGIMLWQSSENRVANNLVHNTPYTGIILSGCMTDFFRRQGRELGRTIR 592 Query: 484 ----------------------NDQVVEYNNIYDVCKTFSDLGAIYMNLGAAPQERGSVI 521 +E N I+ + D AIY+ + G+VI Sbjct: 593 RHEIGSLPKNPKQEDVLPYLHTRGNQIERNEIHHAMEMLGDGNAIYIRGAGS----GNVI 648 Query: 522 RGNYFHHIGESKAGVQGVYPDNFTMGMTIEQNIFYRMGNSAILNNGGSHIRTKNNLFIDA 581 R NY HH+ + D I +N+ Y+ + IL + R +NN+ D Sbjct: 649 RRNYIHHLVAPMIMQAAIRTDGGQRDTLIAENLIYKCTSQGILMK--LNTRVENNIVADI 706 Query: 582 KVPYEYSDMYLGDGP 596 P + + +GP Sbjct: 707 IAPPRGYYLSVREGP 721 >ref|NP_822582.1| hypothetical protein [Streptomyces avermitilis MA-4680] dbj|BAC69117.1| hypothetical protein [Streptomyces avermitilis MA-4680] Length = 1129 Score = 92.0 bits (227), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 145/623 (23%), Positives = 230/623 (36%), Gaps = 162/623 (26%) Query: 37 YVSENGSDSNSGTKDKPFLTLEKARDAIRQLKQGAGLPDGGVTVYLRGGTYNRTGSFLLE 96 YVS G D SG+ PF TLE ARD +R +++ A G + V L GTY + +F L Sbjct: 81 YVSPAGRDVGSGSAAHPFRTLEHARDHVRDVRRNA---KGDIHVRLMSGTYRLSRTFALT 137 Query: 97 EQDSGTADKPVTYKAYPGESVRLSGGRQLQKGWFTPVTDPSVLNRIISTDARSKVLQADL 156 +DSG + Y+A PG +SGG ++ GW + +V +A Sbjct: 138 ARDSGRDGHKIVYEAAPGAHPVISGGERI-TGW-------------VPAGGAGRVYKA-- 181 Query: 157 KGHGITDYGVMSRHGYYKANDVSQTPPMELYIEGEGMTLARWPNKGTVQMGNIIDPGPTR 216 K D+ +LY++GE T AR + +P Sbjct: 182 -----------------KVGDIDT---RQLYVDGELETRAR----------SAKNPSGFS 211 Query: 217 KDADLQTRGGTFTYT----YERPDLWTQADDIWLDGIFGYSWEWSYNKIASIDTQNKSIT 272 K + G TFT T Y+RP D SW W + + + +T Sbjct: 212 K----TSTGYTFTDTGLNAYKRPS----------DLEVVSSWGWKLMRCPAQSIADNKMT 257 Query: 273 L-------AYGEMSGLFKNWYPDFHFAQNLLEEIDMPGEYYIDRSKGKLYFMPTAAFQAA 325 + A+ + +N + +N E +D PGE+Y+D+ +G++Y+MP A + Sbjct: 258 MQQPCWHNAHLQRGQEIQN----PTWLENARELLDTPGEWYLDKGEGEIYYMPKAGQDLS 313 Query: 326 NPEITVSML---------KTPMISTLNTSFVTFEDLILENGRDSGAVIMGGDSVRLVHCE 376 +TV + +T +S ++ VTF +I G R+V + Sbjct: 314 TATVTVPRVQDLVDLDGTRTDPVSNVSFRGVTFSYSTWLAPSSFEGLIEGQAGFRMVGHD 373 Query: 377 IRNFTEGGVRINTQSRWLYNDFAKGTGVNHAI--VSTHIHHVGGTGVILNGGDRLTLAPG 434 +F T+ +W A H I H+G G+ LN G + + G Sbjct: 374 -PDFDA------TRLKWQKTPGAVNVSYGHGIGFEGNTFTHLGAVGLNLNTGTQGSDITG 426 Query: 435 N--------------------------NVVENSHIHDFAYYHKA--YNPAV-ILTGAGNR 465 N ++ +++ + + A YN ++ IL G + Sbjct: 427 NVFRQIAATGIQIGGVAVIDAHPDDSRDITKDTRVDNNVVTKVADQYNGSIGILAGYTDH 486 Query: 466 --ISHNEIHDAPHPGILI-----------------------------FGNDQVVEYNNIY 494 I+HN+++D P+ GI + D V N IY Sbjct: 487 TVITHNKVYDLPYTGISVGWGWGLTDKGGNSNYSPGNLGVPIWDTPTTSRDTEVTDNEIY 546 Query: 495 DVCKTFSDLGAIYMNLGAAPQERGSVIRGNYFHHIGESKAGVQGVYPDNFTMGMTIEQNI 554 D+ K+ +D GA+Y L P G V+ GNY H I G +Y D + T N Sbjct: 547 DIMKSQADGGAVY-TLSTDP---GGVVSGNYIHGIPSPAYGA--IYHDEGSRYWTNTGNA 600 Query: 555 FYRMGNSAILNNGGSHIRTKNNL 577 + + N G I N Sbjct: 601 LCDVAYQWLFLNHGMDIDATGNF 623 >ref|NP_733499.1| putative secreted protein [Streptomyces coelicolor A3(2)] emb|CAD55438.1| putative secreted protein [Streptomyces coelicolor A3(2)] Length = 629 Score = 86.7 bits (213), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 141/602 (23%), Positives = 229/602 (38%), Gaps = 132/602 (21%) Query: 20 PAAAIQAEESHSSEAVYYVSENGSDSNSGTKDKPFLTLEKARDAIRQLKQGAGLP---DG 76 P A+ Q ++E +VS +G+D GTKDKPF T+EKARD IR +G P Sbjct: 35 PDASAQPAGCRATE--LFVSPDGNDKAKGTKDKPFKTIEKARDHIRD--KGLNRPGRMRC 90 Query: 77 GVTVYLRGGTYNRTGSFLLEEQDSGTADKPVTYKAYPGE-SVRLSGGRQLQKGWFTPVTD 135 + V LR G Y + L+++DSG V Y++Y G R++G PVT Sbjct: 91 DIHVNLRAGDYPVNRTVELDDRDSGAGGHQVVYRSYDGPGKARVTGAE--------PVT- 141 Query: 136 PSVLNRIISTDARSKVLQADLKGHGITDYGVMSRHGYYKANDVSQTPPMELYIEGEGMTL 195 G +Y + G Y+A+ + P L+ +G T Sbjct: 142 ------------------------GWEEY----KDGIYRADVGTDNPFYTLFEDGTRATT 173 Query: 196 ARWPNKGTVQ------MGNIIDPGPTRKDADLQTRGGTFTYTYERPDLWTQADDIWLDGI 249 AR+PN+ + + + +P L + G + T+ DL +W G Sbjct: 174 ARYPNRKSEEEWAPYLTSTLPEPEKEAVHQWLYSNPGDWDATW---DLGNAQAVVWSGG- 229 Query: 250 FGYSWEWSYNKIASID--TQNKSITLAYGEMSGLFKNWYPDFHFAQNLLEEIDMPGEYYI 307 SW W + + D +TL Y L + +F QN L+ +D GEYY+ Sbjct: 230 ---SWSWFTDTVPIRDWNPAKNQMTLKYWTRYALNNSGGGSRYFLQNSLDFLDQAGEYYL 286 Query: 308 DRSKGKLYFMPTAAFQAANPEITVSMLKTPMISTL----------NTSFVTFEDLILE-- 355 D KG+LY+ P V++L+ P + TL VT + L L+ Sbjct: 287 DFKKGQLYYKPRGDID------DVTVLR-PTVKTLFDLAGTAPDRRVQNVTLDGLALQYT 339 Query: 356 -------NG----RDSGAVIMGGDSVRLVHCEIRNFTEGGVRIN-----TQSRWLYND-- 397 NG DSG V + R + F G V + T SR +D Sbjct: 340 DFVDWYRNGWISDGDSGEVHKYPEYDRQIEMPRNRF--GAVSVTNSKDVTLSRLKISDTG 397 Query: 398 ----FAKGTGVNHAIVSTHIHHVGGTGVILNGGDRLTLAPGNNVVENSHI--HDFAYYHK 451 +A + + + + ++G G+ + G PG N H+ +++ ++ Sbjct: 398 YHAVYALAANDHLTVRDSLLENIGADGIKVEG-----PYPGEGNTSNGHLLTNNYITHYG 452 Query: 452 AYNPA-----VILTGAGNRISHNEIHDAPHPGILI-----------FGNDQVVEYNNIYD 495 NP ++ N +SH+ + + GI + + EY I + Sbjct: 453 ELNPGDASGIELMNTGHNTVSHSHVKHSARYGISLEVRPEVALKDNYARGNTFEYLRIDE 512 Query: 496 VCKTFSDLGAIYM----NLGAAPQERG--SVIRGNYFHHIGESKAGVQGVYPDNFTMGMT 549 D+GA Y N+ P + ++ G+ +G +G++ D G T Sbjct: 513 AGLDSGDMGAFYTYGVNNVEPYPMDNHVRQMVVGDVIPDESMPDSGTRGIHMDAGGCGFT 572 Query: 550 IE 551 E Sbjct: 573 FE 574 >ref|NP_631229.1| putative secreted sugar hydrolase [Streptomyces coelicolor A3(2)] emb|CAC01659.1| putative secreted sugar hydrolase [Streptomyces coelicolor A3(2)] Length = 786 Score = 77.8 bits (190), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 136/582 (23%), Positives = 218/582 (37%), Gaps = 85/582 (14%) Query: 3 KRTFIVLSIIALIVGCFPAAAIQAEESHSSEAVYYVSENGSDSNSGTKDKPFLTLEKARD 62 +R+ +V+ A V AA+ +H+ YVS GSD+N GTK PF T+++ D Sbjct: 4 RRSALVIGTAAATVLTALTAAVPVS-AHTKGTTRYVSVTGSDANRGTKQSPFSTVQQCAD 62 Query: 63 AIRQLKQGAGLPDGGVTVYLRGGTYNRTGSFLLEEQDSGTADKPVTYKAYPGESVRLSGG 122 + G T + GTY T L GTA +T++A PG V L G Sbjct: 63 VAQ----------AGDTCVIAAGTYRET----LTPPRDGTASARITFRAAPGARVVLDGS 108 Query: 123 RQLQKGWFTPVTDPSVLNRIISTDARSKVLQADLKGHGITDYGVMSRHGYYKANDVSQTP 182 + GW +S S + AD G +D+ + G D++ P Sbjct: 109 APVD-GWAQ-----------VSALDLSALQAADPTLSG-SDFATAATAGKIYRTDLTLNP 155 Query: 183 PM---ELYIEGEGMTLARWPNKGTVQMGNIIDPGPTRKDADLQTRGGTFTYTYERPDLWT 239 + +L+++G + A+WP GT PTR D + GT Y+ D T Sbjct: 156 ALPGNQLFVDGTMLGEAQWPYPGTE---------PTRPDYN-SADAGTDDTLYD--DALT 203 Query: 240 QADDIWLDGIFGYSWEWSYNKIASIDTQNKSITLAYGEMS---GLFKNWYPDFHFAQNLL 296 Q W G S W ++ ++ + A G S GL N + + L Sbjct: 204 QPVGYW-KGARLTSHNWFVSQTGTVTSSAVGSVTASGLASSCVGLSPN-QQNLYSLTGKL 261 Query: 297 EEIDMPGEYYIDRSKGKLY-FMPTAA------FQAANPEITVSMLKTPMISTLNTSFVTF 349 + G++Y D +LY +MP A +A + V + +S + Sbjct: 262 QAFSHAGQWYYDADAQRLYMWMPDGASPTGHTVEAKQRNVAVDLSGRSYVSVVGLGVRAA 321 Query: 350 EDLILENGRDSGAVIMGGDSVRLVHCEIRNFTEGGVR------INTQSRWLYNDFAKGTG 403 + + + V+ G D+ + H G V + T KGT Sbjct: 322 T--VATSSTSTHNVLDGIDASYVSHYMDLKVDPGKVTPADPCDVLTAGETTSGILLKGTA 379 Query: 404 VNHAIVSTHIHHVGGTGVILNGGDRLTLAPGNNVVENSHIHDFAYYHKAYNPAVILTGAG 463 + + ++ I + G GV+L G GN V N + Y +Y + + G+ Sbjct: 380 --NTLRNSRIDYSAGNGVLLAGS-------GNTVTNN--LITRVDYRGSYAAGINVLGSD 428 Query: 464 NRISHNEIHDAPHPGILI-------FGNDQVVEYNNIYDVCKTFSDLGAIYMNLGAAPQE 516 I+HN + ++ I I + + YN++ D D+GAIY+ Sbjct: 429 QTITHNTVTNSGRSNINIDNKVAGTTASGHEIAYNDLSDYGNLVVDVGAIYICCQV--NL 486 Query: 517 RGSVIRGNYFHHIGESKAGVQ--GVYPDNFTMGMTIEQNIFY 556 G+ I N H A GVY D T T+ N+ + Sbjct: 487 AGTEIHHNELHDAALFAASAPAPGVYLDLSTYNATVYDNVAW 528 >ref|NP_391527.1| ywoF [Bacillus subtilis] pir||A70065 hypothetical protein ywoF - Bacillus subtilis emb|CAB05379.1| unknown [Bacillus subtilis] emb|CAB15663.1| ywoF [Bacillus subtilis subsp. subtilis str. 168] Length = 468 Score = 70.1 bits (170), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 50/134 (37%), Positives = 73/134 (54%), Gaps = 16/134 (11%) Query: 1 MKKRTFIVLS-IIALIVGCFPAAAIQAEESHSSEAVYYVSENGSDSNSGTKDKPFLTLEK 59 M+K FI+L+ ++ ++ F +A E S + +Y VS NGSD N GTK+KPF TL Sbjct: 1 MRKWYFILLAGVLTSVILAFVYDKTKANEEGSGDYLY-VSPNGSDQNEGTKEKPFRTLAH 59 Query: 60 ARDAIRQLKQGAGLPDGGVTVYLRGGTYNRTGSFLLEEQDSGTADKPVTYKAYPGESVRL 119 A + K A G TV +R GTY+ T L+ + SGT KP+T++ Y E+V + Sbjct: 60 ASE-----KAAA-----GTTVMIREGTYHET----LDVKHSGTDGKPITFRNYENENVVI 105 Query: 120 SGGRQLQKGWFTPV 133 SG + TP+ Sbjct: 106 SGESVANAEYETPL 119 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.135 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 2,010,693,976 Number of extensions: 94720523 Number of successful extensions: 193321 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 193343 Number of HSP's successfully gapped: 32 Length of query: 1216 Length of database: 757,237,578 Length adjustment: 137 Effective length of query: 1079 Effective length of database: 385,269,015 Effective search space: 415705267185 Effective search space used: 415705267185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)