BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0018.p (484 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAC27412.2| endo-1,3(4)-beta-glucanase [Clostridium thermoce... 159 2e-37 ref|ZP_00060160.1| COG2273: Beta-glucanase/Beta-glucan synthetas... 159 2e-37 pir||T18265 endo-1,3(4)-beta-glucanase (EC 3.2.1.6) - Clostridiu... 159 2e-37 ref|ZP_00062117.1| hypothetical protein [Clostridium thermocellu... 138 2e-31 ref|ZP_00061491.1| hypothetical protein [Clostridium thermocellu... 110 9e-23 >emb|CAC27412.2| endo-1,3(4)-beta-glucanase [Clostridium thermocellum] emb|CAA61884.2| endo-1,3(4)-beta-glucanase [Clostridium thermocellum] Length = 1321 Score = 159 bits (401), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 77/173 (44%), Positives = 108/173 (62%), Gaps = 1/173 (0%) Query: 29 VSAAPVPKDIQGHWAQKQLQDWLNKGYLGGYQDGTVKPNKAITRAEYVALVNRLFGYTDT 88 V A+ DI+GHWA++ L W+ KG L GYQDGT++P+ ITRAE+V L+N++FG + Sbjct: 24 VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTIRPDNNITRAEFVTLINKVFGLYEL 83 Query: 89 ATISFKDLKKSNWYYSEVAKAVKAGYIGGYENNTFRPNASLTRQEAAVIAAKILKLNTKA 148 + F D++ S WY E+ KA AGYI GY +N F+P+ +TRQEA VI AK+ +L + + Sbjct: 84 SREQFADVEDSKWYSREILKARAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGS 143 Query: 149 T-TAKFKDSSQFXXXXXXXXXXXXDKKVINGYPDGTFGPKRALTRAEAVGIIS 200 T+KFKD S +K I GY DGTF P +TRAE + I++ Sbjct: 144 NYTSKFKDGSLVKEYAKDSVSALVEKGYIAGYEDGTFRPDNYITRAETIKILN 196 >ref|ZP_00060160.1| COG2273: Beta-glucanase/Beta-glucan synthetase [Clostridium thermocellum ATCC 27405] Length = 1321 Score = 159 bits (401), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 77/173 (44%), Positives = 108/173 (62%), Gaps = 1/173 (0%) Query: 29 VSAAPVPKDIQGHWAQKQLQDWLNKGYLGGYQDGTVKPNKAITRAEYVALVNRLFGYTDT 88 V A+ DI+GHWA++ L W+ KG L GYQDGT++P+ ITRAE+V L+N++FG + Sbjct: 24 VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTIRPDNNITRAEFVTLINKVFGLYEL 83 Query: 89 ATISFKDLKKSNWYYSEVAKAVKAGYIGGYENNTFRPNASLTRQEAAVIAAKILKLNTKA 148 + F D++ S WY E+ KA AGYI GY +N F+P+ +TRQEA VI AK+ +L + + Sbjct: 84 SREQFADVEDSKWYSREILKARAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGS 143 Query: 149 T-TAKFKDSSQFXXXXXXXXXXXXDKKVINGYPDGTFGPKRALTRAEAVGIIS 200 T+KFKD S +K I GY DGTF P +TRAE + I++ Sbjct: 144 NYTSKFKDGSLVKEYAKDSVSALVEKGYIAGYEDGTFRPDNYITRAETIKILN 196 >pir||T18265 endo-1,3(4)-beta-glucanase (EC 3.2.1.6) - Clostridium thermocellum Length = 1324 Score = 159 bits (401), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 77/173 (44%), Positives = 108/173 (62%), Gaps = 1/173 (0%) Query: 29 VSAAPVPKDIQGHWAQKQLQDWLNKGYLGGYQDGTVKPNKAITRAEYVALVNRLFGYTDT 88 V A+ DI+GHWA++ L W+ KG L GYQDGT++P+ ITRAE+V L+N++FG + Sbjct: 24 VQASDGINDIRGHWAEEDLNKWMEKGILVGYQDGTIRPDNNITRAEFVTLINKVFGLYEL 83 Query: 89 ATISFKDLKKSNWYYSEVAKAVKAGYIGGYENNTFRPNASLTRQEAAVIAAKILKLNTKA 148 + F D++ S WY E+ KA AGYI GY +N F+P+ +TRQEA VI AK+ +L + + Sbjct: 84 SREQFADVEDSKWYSREILKARAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGS 143 Query: 149 T-TAKFKDSSQFXXXXXXXXXXXXDKKVINGYPDGTFGPKRALTRAEAVGIIS 200 T+KFKD S +K I GY DGTF P +TRAE + I++ Sbjct: 144 NYTSKFKDGSLVKEYAKDSVSALVEKGYIAGYEDGTFRPDNYITRAETIKILN 196 >ref|ZP_00062117.1| hypothetical protein [Clostridium thermocellum ATCC 27405] ref|ZP_00062252.1| hypothetical protein [Clostridium thermocellum ATCC 27405] ref|ZP_00062263.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 223 Score = 138 bits (348), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 67/174 (38%), Positives = 104/174 (59%), Gaps = 4/174 (2%) Query: 36 KDIQGHWAQKQLQDWLNKGYLGGYQDGTVKPNKAITRAEYVALVNRLFGYTDTATI--SF 93 DI+GHWAQ ++ G + GY+D + +P++++TRAE+V + NR F + SF Sbjct: 13 NDIEGHWAQFWIELNAKLGNIAGYEDSSFRPSQSVTRAEFVTMANRFFDIRGSVEFGCSF 72 Query: 94 KDLKKSNWYYSEVAKAVKAGYIGGYENNTFRPNASLTRQEAAVIAAKILKLN--TKATTA 151 D+ +++W++++VA A KAGYI GY + TFRPN +TR+EA+VI K++ + T A Sbjct: 73 TDVNENDWFFADVATAEKAGYISGYPDGTFRPNELITREEASVILCKLINSDPRTHADNI 132 Query: 152 KFKDSSQFXXXXXXXXXXXXDKKVINGYPDGTFGPKRALTRAEAVGIISNSVAQ 205 +F DS +I GYPD TF PK +TRAEAV ++ N++ + Sbjct: 133 EFTDSDSISPWAYGYVNSLVYNHIIKGYPDNTFRPKNPVTRAEAVVLLDNALMR 186 >ref|ZP_00061491.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 4596 Score = 110 bits (274), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 60/171 (35%), Positives = 87/171 (50%), Gaps = 4/171 (2%) Query: 37 DIQGHWAQKQLQDWLNKGYLGGYQDGTVKPNKAITRAEYVALVNRLFGYTDTATISFKDL 96 DI+GHW + D L + + GY D T++PN ITRAE +V + G+ + +I Sbjct: 4421 DIKGHWTAEFFADLLRREIVNGYPDRTLRPNAPITRAEATVMVVKAAGFEVSDSIPLTCT 4480 Query: 97 KKSN---WYYSEVAKAVKAGYIGGYENNTFRPNASLTRQEAAVIAAKILKLNT-KATTAK 152 K + W VA A+ G + GYE+ +FRP+ LTRQE V+ + + + T Sbjct: 4481 DKDSVPAWAKPYVATAMHKGVVKGYEDGSFRPSNRLTRQETVVLVLRAFGIEEAQDKTLA 4540 Query: 153 FKDSSQFXXXXXXXXXXXXDKKVINGYPDGTFGPKRALTRAEAVGIISNSV 203 F DS + + +I GY D TFGP+R +TRAE V IIS + Sbjct: 4541 FADSDKIPAWSRGYIKKAVELGIIKGYSDNTFGPQREITRAEVVTIISKCI 4591 Score = 77.4 bits (189), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 42/112 (37%), Positives = 62/112 (55%), Gaps = 3/112 (2%) Query: 36 KDIQGHWAQKQLQDWLNKGYLGGYQDGTVKPNKAITRAEYVALVNRLFGYTDTA--TISF 93 KD WA+ + ++KG + GY+DG+ +P+ +TR E V LV R FG + T++F Sbjct: 4482 KDSVPAWAKPYVATAMHKGVVKGYEDGSFRPSNRLTRQETVVLVLRAFGIEEAQDKTLAF 4541 Query: 94 KDLKK-SNWYYSEVAKAVKAGYIGGYENNTFRPNASLTRQEAAVIAAKILKL 144 D K W + KAV+ G I GY +NTF P +TR E I +K ++L Sbjct: 4542 ADSDKIPAWSRGYIKKAVELGIIKGYSDNTFGPQREITRAEVVTIISKCIQL 4593 Score = 55.1 bits (131), Expect = 4e-06, Method: Compositional matrix adjust. Identities = 34/115 (29%), Positives = 49/115 (42%), Gaps = 1/115 (0%) Query: 86 TDTATISFKDLKKSNWYYSEVAKAVKAGYIGGYENNTFRPNASLTRQEAAVIAAKILKLN 145 TDT ++ F K +W A ++ + GY + T RPNA +TR EA V+ K Sbjct: 4411 TDTNSLDFFTDIKGHWTAEFFADLLRREIVNGYPDRTLRPNAPITRAEATVMVVKAAGFE 4470 Query: 146 -TKATTAKFKDSSQFXXXXXXXXXXXXDKKVINGYPDGTFGPKRALTRAEAVGII 199 + + D K V+ GY DG+F P LTR E V ++ Sbjct: 4471 VSDSIPLTCTDKDSVPAWAKPYVATAMHKGVVKGYEDGSFRPSNRLTRQETVVLV 4525 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.128 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 551,704,912 Number of extensions: 18627276 Number of successful extensions: 37713 Number of sequences better than 1.0e-05: 127 Number of HSP's gapped: 37387 Number of HSP's successfully gapped: 165 Length of query: 484 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 354 Effective length of database: 404,274,708 Effective search space: 143113246632 Effective search space used: 143113246632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 128 (53.9 bits)