BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0013.p (339 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_391409.1| peptide chain release factor 2 [Bacillus subtil... 505 e-142 ref|NP_981586.1| peptide chain release factor 2, programmed fram... 500 e-140 ref|NP_834852.1| Bacterial Peptide Chain Release Factor 2 (RF-2)... 488 e-136 ref|NP_653635.1| RF-1, Peptidyl-tRNA hydrolase domain [Bacillus ... 485 e-136 ref|NP_693416.1| peptide chain release factor RF-2 [Oceanobacill... 481 e-134 >ref|NP_391409.1| peptide chain release factor 2 [Bacillus subtilis] sp|P28367|RF2_BACSU Peptide chain release factor 2 (RF-2) pir||JN0146 translation releasing factor RF-2 - Bacillus subtilis gb|AAC97534.1| release factor 2 [Bacillus subtilis] gb|AAC67303.1| putative peptide chain release factor RF-2 [Bacillus subtilis] emb|CAB15546.1| peptide chain release factor 2 [Bacillus subtilis subsp. subtilis str. 168] Length = 366 Score = 505 bits (1301), Expect = e-142, Method: Compositional matrix adjust. Identities = 232/333 (69%), Positives = 276/333 (82%) Query: 2 IANFEEKMAAPGFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXX 61 IA +E+MA P FW+D +KAQ VI E N +K V+ Y+KL + +++ M Sbjct: 33 IAELDEQMADPEFWNDQQKAQTVINEANGLKDYVNSYKKLNESHEELQMTHDLLKEEPDT 92 Query: 62 XXXXXXXXTIRSVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYT 121 ++S+ + +EFELQLLL++PYDK NAILELHPGAGGTESQDWG MLLRMYT Sbjct: 93 DLQLELEKELKSLTKEFNEFELQLLLSEPYDKNNAILELHPGAGGTESQDWGSMLLRMYT 152 Query: 122 RWAEKRGFKVEVLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRR 181 RW E+RGFKVE LDYLPGDEAGIKSVTLLIKG+N YGYLKAEKGVHRL RISPFD+SGRR Sbjct: 153 RWGERRGFKVETLDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRR 212 Query: 182 HTSFVSCDVVPEITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVT 241 HTSFVSC+V+PE D+++++IRTED+K+DTYRASGAGGQH+NTTDSAVRITHLPT VVVT Sbjct: 213 HTSFVSCEVMPEFNDEIDIDIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTNVVVT 272 Query: 242 CQNERSQIKNREQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSM 301 CQ ERSQIKNRE+AMKML++KLY+R+++EQQ +LDEIRGEQ +I WGSQIRSYVFHPYSM Sbjct: 273 CQTERSQIKNRERAMKMLKAKLYQRRIEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSM 332 Query: 302 VKDHRTSVETGNVGAVMDGDLDAFIDGYLRSQI 334 VKDHRT+ E GNV AVMDGD+D FID YLRS++ Sbjct: 333 VKDHRTNTEMGNVQAVMDGDIDTFIDAYLRSKL 365 >ref|NP_981586.1| peptide chain release factor 2, programmed frameshift [Bacillus cereus ATCC 10987] gb|AAS44194.1| peptide chain release factor 2, programmed frameshift [Bacillus cereus ATCC 10987] Length = 380 Score = 500 bits (1287), Expect = e-140, Method: Compositional matrix adjust. Identities = 233/333 (69%), Positives = 272/333 (81%) Query: 2 IANFEEKMAAPGFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXX 61 IA EEKM GFWDD + AQ VI E NA+K V ++ +L + +++ + Sbjct: 48 IAELEEKMMGAGFWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDE 107 Query: 62 XXXXXXXXTIRSVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYT 121 ++ + +++E+ELQLLL+ PYDK NAILELHPGAGGTESQDWG MLLRMYT Sbjct: 108 DLHEELESEVKGLIQEMNEYELQLLLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYT 167 Query: 122 RWAEKRGFKVEVLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRR 181 RWAEKRGFKVE +DYLPGDEAGIKSVTLLIKG+N YGYLKAEKGVHRL RISPFD+SGRR Sbjct: 168 RWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRR 227 Query: 182 HTSFVSCDVVPEITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVT 241 HTSFVSC+VVPE D+VE+E+RTEDLKIDTYRASGAGGQH+NTTDSAVRITH PT VVT Sbjct: 228 HTSFVSCEVVPEFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVT 287 Query: 242 CQNERSQIKNREQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSM 301 CQ+ERSQIKNRE AMKML++KLY++KL+EQQ +LDEIRGEQ +I WGSQIRSYVFHPYS+ Sbjct: 288 CQSERSQIKNREHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSL 347 Query: 302 VKDHRTSVETGNVGAVMDGDLDAFIDGYLRSQI 334 VKDHRT+ E GNV AVMDG++D FID YLRS+I Sbjct: 348 VKDHRTNTEVGNVQAVMDGEIDPFIDAYLRSRI 380 >ref|NP_834852.1| Bacterial Peptide Chain Release Factor 2 (RF-2) [Bacillus cereus ATCC 14579] gb|AAP12053.1| Bacterial Peptide Chain Release Factor 2 (RF-2) [Bacillus cereus ATCC 14579] Length = 325 Score = 488 bits (1255), Expect = e-136, Method: Compositional matrix adjust. Identities = 227/322 (70%), Positives = 266/322 (82%) Query: 13 GFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXXXXXXXXXXTIR 72 GFWDD + AQ VI E NA+K V ++ +L + +++ + ++ Sbjct: 4 GFWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELESEVK 63 Query: 73 SVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYTRWAEKRGFKVE 132 + +++E+ELQLLL+ PYDK NAILELHPGAGGTESQDWG MLLRMYTRWAEKRGFKVE Sbjct: 64 GLIQEMNEYELQLLLSDPYDKNNAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVE 123 Query: 133 VLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRRHTSFVSCDVVP 192 +DYLPGDEAGIKSVTLLIKG+N YGYLKAEKGVHRL RISPFD+SGRRHTSFVSC+VVP Sbjct: 124 TVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSCEVVP 183 Query: 193 EITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVTCQNERSQIKNR 252 E D+VE+E+RTEDLKIDTYRASGAGGQH+NTTDSAVRITH PT VVTCQ+ERSQIKNR Sbjct: 184 EFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQIKNR 243 Query: 253 EQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSMVKDHRTSVETG 312 E AMKML++KLY++KL+EQQ +LDEIRGEQ +I WGSQIRSYVFHPYS+VKDHRT+ E G Sbjct: 244 EHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVG 303 Query: 313 NVGAVMDGDLDAFIDGYLRSQI 334 NV AVMDG++D FID YLRS+I Sbjct: 304 NVQAVMDGEIDPFIDAYLRSRI 325 >ref|NP_653635.1| RF-1, Peptidyl-tRNA hydrolase domain [Bacillus anthracis A2012] Length = 325 Score = 485 bits (1248), Expect = e-136, Method: Compositional matrix adjust. Identities = 226/322 (70%), Positives = 265/322 (82%) Query: 13 GFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXXXXXXXXXXTIR 72 GFWDD + AQ VI E NA+K V ++ +L + +++ + ++ Sbjct: 4 GFWDDQQGAQAVINEANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELESEVK 63 Query: 73 SVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYTRWAEKRGFKVE 132 + +++E+ELQLLL+ PYDK AILELHPGAGGTESQDWG MLLRMYTRWAEKRGFKVE Sbjct: 64 GLIQEMNEYELQLLLSDPYDKNKAILELHPGAGGTESQDWGSMLLRMYTRWAEKRGFKVE 123 Query: 133 VLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRRHTSFVSCDVVP 192 +DYLPGDEAGIKSVTLLIKG+N YGYLKAEKGVHRL RISPFD+SGRRHTSFVSC+VVP Sbjct: 124 TVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSCEVVP 183 Query: 193 EITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVTCQNERSQIKNR 252 E D+VE+E+RTEDLKIDTYRASGAGGQH+NTTDSAVRITH PT VVTCQ+ERSQIKNR Sbjct: 184 EFNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQIKNR 243 Query: 253 EQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSMVKDHRTSVETG 312 E AMKML++KLY++KL+EQQ +LDEIRGEQ +I WGSQIRSYVFHPYS+VKDHRT+ E G Sbjct: 244 EHAMKMLKAKLYQKKLEEQQAELDEIRGEQKEIGWGSQIRSYVFHPYSLVKDHRTNTEVG 303 Query: 313 NVGAVMDGDLDAFIDGYLRSQI 334 NV AVMDG++D FID YLRS+I Sbjct: 304 NVQAVMDGEIDPFIDAYLRSRI 325 >ref|NP_693416.1| peptide chain release factor RF-2 [Oceanobacillus iheyensis HTE831] dbj|BAC14451.1| peptide chain release factor RF-2 [Oceanobacillus iheyensis HTE831] Length = 327 Score = 481 bits (1237), Expect = e-134, Method: Compositional matrix adjust. Identities = 221/326 (67%), Positives = 264/326 (80%) Query: 9 MAAPGFWDDPEKAQGVIAEMNAVKSSVDQYEKLQQEYDDAAMMXXXXXXXXXXXXXXXXX 68 MA P FWDD KAQ VI E N +K V+ +E +++ ++ + Sbjct: 1 MADPSFWDDQNKAQSVIQETNGIKEYVNGFETIEENLENLEVSYELVKEENDQELFADLD 60 Query: 69 XTIRSVGGKVDEFELQLLLNQPYDKLNAILELHPGAGGTESQDWGQMLLRMYTRWAEKRG 128 ++ V++FELQLLL++PYD NAILELHPGAGGTESQDW MLLRMY RWAE+R Sbjct: 61 AELQQFITDVNQFELQLLLSEPYDANNAILELHPGAGGTESQDWASMLLRMYQRWAEERN 120 Query: 129 FKVEVLDYLPGDEAGIKSVTLLIKGYNVYGYLKAEKGVHRLGRISPFDASGRRHTSFVSC 188 FKVE LDYLPGDEAG+KSVTL+IKG+N YGYLKAEKGVHRL RISPFD+SGRRHTSFVSC Sbjct: 121 FKVETLDYLPGDEAGLKSVTLIIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFVSC 180 Query: 189 DVVPEITDDVEVEIRTEDLKIDTYRASGAGGQHINTTDSAVRITHLPTGVVVTCQNERSQ 248 DV PE++DDV+++IRTED+K+DTYRASGAGGQH+NTTDSAVRITHLPT ++VTCQNERSQ Sbjct: 181 DVTPEMSDDVDIDIRTEDIKVDTYRASGAGGQHVNTTDSAVRITHLPTNIIVTCQNERSQ 240 Query: 249 IKNREQAMKMLRSKLYERKLQEQQQQLDEIRGEQSDIAWGSQIRSYVFHPYSMVKDHRTS 308 IKNRE AMKML+SKLY+ ++++Q+Q+LD+IRGEQ +I WGSQIRSYVFHPYSMVKDHRT Sbjct: 241 IKNREAAMKMLKSKLYQLEVEKQRQELDDIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTD 300 Query: 309 VETGNVGAVMDGDLDAFIDGYLRSQI 334 ETGN AVMDGD++ FID YLRSQ+ Sbjct: 301 KETGNTQAVMDGDINPFIDAYLRSQM 326 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 471,178,630 Number of extensions: 18253601 Number of successful extensions: 65414 Number of sequences better than 1.0e-05: 1352 Number of HSP's gapped: 64272 Number of HSP's successfully gapped: 1355 Length of query: 339 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 213 Effective length of database: 415,135,104 Effective search space: 88423777152 Effective search space used: 88423777152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)