BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0515.0004.p (411 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00129406.1| COG0137: Argininosuccinate synthase [Desulfov... 524 e-147 ref|NP_714346.1| argininosuccinate synthase [Leptospira interrog... 511 e-143 gb|AAS71866.1| argininosuccinate synthase; citrulline-aspartate ... 511 e-143 sp|P59846|ASSY_THETH Argininosuccinate synthase (Citrulline--asp... 509 e-143 ref|ZP_00013177.1| COG0137: Argininosuccinate synthase [Rhodospi... 496 e-139 >ref|ZP_00129406.1| COG0137: Argininosuccinate synthase [Desulfovibrio desulfuricans G20] Length = 400 Score = 524 bits (1350), Expect = e-147, Method: Compositional matrix adjust. Identities = 248/399 (62%), Positives = 321/399 (80%), Gaps = 11/399 (2%) Query: 4 EKIVLAYSGGLDTSVILKWLKETYDAEIIAFTADIGQKEELNGLEEKALATGASKVYIDD 63 +K+VLAYSGGLDTSVILKW+ +TYD E++ TAD+GQ+E+L+G++EKAL TGA++ Y++D Sbjct: 6 KKVVLAYSGGLDTSVILKWIAKTYDCEVVTLTADLGQEEDLDGVDEKALRTGATRAYVED 65 Query: 64 LRDEFASDFIYPMFQSGALYEGQYLLGTSIARPLIAKRMVDIAIAEGATAIAHGATGKGN 123 LR+EFA DFI+PM ++ A+YEG+YLLGTSIARPLI +R+V+IA AEGA A+AHGATGKGN Sbjct: 66 LREEFARDFIFPMMRACAIYEGRYLLGTSIARPLITRRLVEIAHAEGAQAVAHGATGKGN 125 Query: 124 DQVRFELNAAALSPSIKVIAPWRLEEFRSQFPGRAEMIAYAEANGIPV-QASAAKPYSMD 182 DQVRFEL +AAL PS+KVIAPWR +FRS R +++ +A N IPV A K YSMD Sbjct: 126 DQVRFELASAALDPSLKVIAPWREWDFRS----RTDLLNFAAENDIPVISAGNRKLYSMD 181 Query: 183 RNLLHISYESGVLEDPWFDPSAPENKGMFLLSNAPEDAPDQPEYLELEFLKGDCVALNGE 242 RN+LH S+E G LEDPW +P G ++++ E APD+PEY+ ++F KG+ VA+NG+ Sbjct: 182 RNMLHCSFEGGELEDPWNEPG----PGSYIMAVPVEQAPDEPEYITIDFEKGNAVAINGQ 237 Query: 243 PLSPLQVMEKLNELGGKHGIGRVDMVENRFVGMKSRGVYETPGGTILFTAHRKMESITMD 302 +SP +++ LN LGGKHG+GR+DMVENRFVGMKSRGVYETPGGT+L AHR +E I +D Sbjct: 238 AMSPAAIIKTLNTLGGKHGVGRLDMVENRFVGMKSRGVYETPGGTVLHAAHRDLEGICLD 297 Query: 303 REVMNLRDSLITRYSTLVYNGFWFAPERLALQALVKESQKNVTGTVRVKLYKGNIIGAGV 362 RE ++LRDSLI RY+ VYNGFW++PER ALQALV ++Q+NVTGTVRVKLYKGN++ G Sbjct: 298 REALHLRDSLIPRYAAAVYNGFWYSPEREALQALVDKTQENVTGTVRVKLYKGNVVPVGR 357 Query: 363 KSPVSLYNPEIATMEADPTQAYDQGDATGFIRLNALRLK 401 KSP SLY ++AT E D + Y+Q DA GFIRLN+LR++ Sbjct: 358 KSPYSLYRMDLATFEED--EVYNQADAAGFIRLNSLRIQ 394 >ref|NP_714346.1| argininosuccinate synthase [Leptospira interrogans serovar lai str. 56601] sp|Q8EYP7|ASSY_LEPIN Argininosuccinate synthase (Citrulline--aspartate ligase) gb|AAN51364.1| argininosuccinate synthase [Leptospira interrogans serovar lai str. 56601] Length = 403 Score = 511 bits (1316), Expect = e-143, Method: Compositional matrix adjust. Identities = 246/397 (61%), Positives = 309/397 (77%), Gaps = 10/397 (2%) Query: 2 PKEKIVLAYSGGLDTSVILKWLKETYDAEIIAFTADIGQKEELNGLEEKALATGASKVYI 61 P +KIVLAYSGGLDTSVIL WLK+TY E+IAFTADIGQKEEL+GLEEK + TGASKVYI Sbjct: 6 PVKKIVLAYSGGLDTSVILTWLKDTYGCEVIAFTADIGQKEELSGLEEKGIKTGASKVYI 65 Query: 62 DDLRDEFASDFIYPMFQSGALYEGQYLLGTSIARPLIAKRMVDIAIAEGATAIAHGATGK 121 DLR EFA DFI+P Q A+YE +YLLGTS+ARPLIAK MV++A EGA A AHGATGK Sbjct: 66 QDLRLEFARDFIFPAIQGNAIYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAHGATGK 125 Query: 122 GNDQVRFELNAAALSPSIKVIAPWRLEEFRSQFPGRAEMIAYAEANGIPVQASAAKPYSM 181 GNDQVRFEL +L+P +IAPWR+ F GR+++I YA++ GIPV +A KPYSM Sbjct: 126 GNDQVRFELGVKSLAPEKTIIAPWRI----WSFGGRSDLIEYAKSKGIPVPVTAEKPYSM 181 Query: 182 DRNLLHISYESGVLEDPWFDPSAPENKGMFLLSNAPEDAPDQPEYLELEFLKGDCVALNG 241 DRNL+HISYE G+LEDP+ +P ++ MFLL+ +PE APD PEYLEL+F +G+CVA+NG Sbjct: 182 DRNLMHISYEGGILEDPYKEP----DEKMFLLTTSPEKAPDAPEYLELDFEEGNCVAING 237 Query: 242 EPLSPLQVMEKLNELGGKHGIGRVDMVENRFVGMKSRGVYETPGGTILFTAHRKMESITM 301 + ++PL++ME LN + GKHG+GRVD+VENR VG+KSRGVYETPGGTILF AHR +ESIT+ Sbjct: 238 KKMNPLEIMETLNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTILFLAHRDLESITI 297 Query: 302 DREVMNLRDSLITRYSTLVYNGFWFAPERLALQALVKESQKNVTGTVRVKLYKGNIIGAG 361 DR+ + +D L ++ L+YNG WF+ A++A + E+Q+ V+GTVR+KLYKG G Sbjct: 298 DRDTQHHKDKLSIEFAELIYNGHWFSSRMKAVRAFITETQRYVSGTVRIKLYKGVCSVVG 357 Query: 362 VKSPVSLYNPEIATMEADPTQAYDQGDATGFIRLNAL 398 KS VSLYNPE+AT E + + Y+Q DA GFI + L Sbjct: 358 RKSQVSLYNPEMATFEKE--ELYNQKDAEGFINIYGL 392 >gb|AAS71866.1| argininosuccinate synthase; citrulline-aspartate ligase [Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130] Length = 403 Score = 511 bits (1316), Expect = e-143, Method: Compositional matrix adjust. Identities = 246/397 (61%), Positives = 309/397 (77%), Gaps = 10/397 (2%) Query: 2 PKEKIVLAYSGGLDTSVILKWLKETYDAEIIAFTADIGQKEELNGLEEKALATGASKVYI 61 P +KIVLAYSGGLDTSVIL WLK+TY E+IAFTADIGQKEEL+GLEEK + TGASKVYI Sbjct: 6 PVKKIVLAYSGGLDTSVILTWLKDTYGCEVIAFTADIGQKEELSGLEEKGIKTGASKVYI 65 Query: 62 DDLRDEFASDFIYPMFQSGALYEGQYLLGTSIARPLIAKRMVDIAIAEGATAIAHGATGK 121 DLR EFA DFI+P Q A+YE +YLLGTS+ARPLIAK MV++A EGA A AHGATGK Sbjct: 66 QDLRLEFARDFIFPAIQGNAIYEMRYLLGTSLARPLIAKAMVEVAEKEGADAFAHGATGK 125 Query: 122 GNDQVRFELNAAALSPSIKVIAPWRLEEFRSQFPGRAEMIAYAEANGIPVQASAAKPYSM 181 GNDQVRFEL +L+P +IAPWR+ F GR+++I YA++ GIPV +A KPYSM Sbjct: 126 GNDQVRFELGVKSLAPEKTIIAPWRI----WSFGGRSDLIEYAKSKGIPVPVTAEKPYSM 181 Query: 182 DRNLLHISYESGVLEDPWFDPSAPENKGMFLLSNAPEDAPDQPEYLELEFLKGDCVALNG 241 DRNL+HISYE G+LEDP+ +P ++ MFLL+ +PE APD PEYLEL+F +G+CVA+NG Sbjct: 182 DRNLMHISYEGGILEDPYKEP----DEKMFLLTTSPEKAPDAPEYLELDFEEGNCVAING 237 Query: 242 EPLSPLQVMEKLNELGGKHGIGRVDMVENRFVGMKSRGVYETPGGTILFTAHRKMESITM 301 + ++PL++ME LN + GKHG+GRVD+VENR VG+KSRGVYETPGGTILF AHR +ESIT+ Sbjct: 238 KKMNPLEIMETLNTIAGKHGVGRVDIVENRLVGIKSRGVYETPGGTILFLAHRDLESITI 297 Query: 302 DREVMNLRDSLITRYSTLVYNGFWFAPERLALQALVKESQKNVTGTVRVKLYKGNIIGAG 361 DR+ + +D L ++ L+YNG WF+ A++A + E+Q+ V+GTVR+KLYKG G Sbjct: 298 DRDTQHHKDKLSIEFAELIYNGHWFSSRMKAVRAFITETQRYVSGTVRIKLYKGICSVVG 357 Query: 362 VKSPVSLYNPEIATMEADPTQAYDQGDATGFIRLNAL 398 KS VSLYNPE+AT E + + Y+Q DA GFI + L Sbjct: 358 RKSQVSLYNPEMATFEKE--ELYNQKDAEGFINIYGL 392 >sp|P59846|ASSY_THETH Argininosuccinate synthase (Citrulline--aspartate ligase) pdb|1KH1|A Chain A, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase pdb|1KH1|B Chain B, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase pdb|1KH1|C Chain C, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase pdb|1KH1|D Chain D, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase pdb|1KH2|A Chain A, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Atp pdb|1KH2|B Chain B, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Atp pdb|1KH2|C Chain C, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Atp pdb|1KH2|D Chain D, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Atp pdb|1KOR|A Chain A, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Inhibitors pdb|1KOR|B Chain B, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Inhibitors pdb|1KOR|C Chain C, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Inhibitors pdb|1KOR|D Chain D, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Inhibitors pdb|1J1Z|A Chain A, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Substrate pdb|1J1Z|B Chain B, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Substrate pdb|1J1Z|C Chain C, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Substrate pdb|1J1Z|D Chain D, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Substrate pdb|1J20|A Chain A, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Product pdb|1J20|B Chain B, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Product pdb|1J20|C Chain C, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Product pdb|1J20|D Chain D, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Product pdb|1J21|A Chain A, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Atp And Citrulline pdb|1J21|B Chain B, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Atp And Citrulline pdb|1J21|C Chain C, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Atp And Citrulline pdb|1J21|D Chain D, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Atp And Citrulline pdb|1KH3|A Chain A, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Inhibitor pdb|1KH3|B Chain B, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Inhibitor pdb|1KH3|C Chain C, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Inhibitor pdb|1KH3|D Chain D, Crystal Structure Of Thermus Thermophilus Hb8 Argininosuccinate Synthetase In Complex With Inhibitor Length = 400 Score = 509 bits (1312), Expect = e-143, Method: Compositional matrix adjust. Identities = 261/402 (64%), Positives = 310/402 (77%), Gaps = 10/402 (2%) Query: 5 KIVLAYSGGLDTSVILKWLKETYDAEIIAFTADIGQKEELNGLEEKALATGASKVYIDDL 64 KIVLAYSGGLDTS+ILKWLKETY AE+IAFTADIGQ EE+ EKAL TGASK DL Sbjct: 2 KIVLAYSGGLDTSIILKWLKETYRAEVIAFTADIGQGEEVEEAREKALRTGASKAIALDL 61 Query: 65 RDEFASDFIYPMFQSGALYEGQYLLGTSIARPLIAKRMVDIAIAEGATAIAHGATGKGND 124 ++EF DF++PM ++GA+YEG YLLGTSIARPLIAK +V IA EGA AIAHGATGKGND Sbjct: 62 KEEFVRDFVFPMMRAGAVYEGYYLLGTSIARPLIAKHLVRIAEEEGAEAIAHGATGKGND 121 Query: 125 QVRFELNAAALSPSIKVIAPWRLEEFRSQFPGRAEMIAYAEANGIPVQASAAKPYSMDRN 184 QVRFEL A AL P IKVIAPWR F GR EMIAYAEA+GIPV + KPYSMD N Sbjct: 122 QVRFELTAYALKPDIKVIAPWR----EWSFQGRKEMIAYAEAHGIPVPVTQEKPYSMDAN 177 Query: 185 LLHISYESGVLEDPWFDPSAPENKGMFLLSNAPEDAPDQPEYLELEFLKGDCVALNGEPL 244 LLHISYE GVLEDPW +P KGMF ++ PE+APD PEY+E+EF +GD VA+NGE L Sbjct: 178 LLHISYEGGVLEDPWAEPP----KGMFRMTQDPEEAPDAPEYVEVEFFEGDPVAVNGERL 233 Query: 245 SPLQVMEKLNELGGKHGIGRVDMVENRFVGMKSRGVYETPGGTILFTAHRKMESITMDRE 304 SP ++++LNE+GG+HG+GRVD+VENRFVGMKSRGVYETPGGTIL+ A R +ES+T+DRE Sbjct: 234 SPAALLQRLNEIGGRHGVGRVDIVENRFVGMKSRGVYETPGGTILYHARRAVESLTLDRE 293 Query: 305 VMNLRDSLITRYSTLVYNGFWFAPERLALQALVKESQKNVTGTVRVKLYKGNIIGAGVKS 364 V++ RD L +Y+ LVY GFW+APER ALQA ++VTG R+KLYKGN+ G K+ Sbjct: 294 VLHQRDMLSPKYAELVYYGFWYAPEREALQAYFDHVARSVTGVARLKLYKGNVYVVGRKA 353 Query: 365 PVSLYNPEIATMEADPTQAYDQGDATGFIRLNALRLKVSAGV 406 P SLY ++ + D YDQ DA GFI++ ALRL+V A V Sbjct: 354 PKSLYRQDLVSF--DEAGGYDQKDAEGFIKIQALRLRVRALV 393 >ref|ZP_00013177.1| COG0137: Argininosuccinate synthase [Rhodospirillum rubrum] Length = 411 Score = 496 bits (1278), Expect = e-139, Method: Compositional matrix adjust. Identities = 238/401 (59%), Positives = 305/401 (76%), Gaps = 12/401 (2%) Query: 4 EKIVLAYSGGLDTSVILKWLKETYDAEIIAFTADIGQKEELNGLEEKALATGASKVYIDD 63 +K+VLAYSGGLDTS+IL+WL++ YD E++ FTADIGQ EEL +KA G ++YI+D Sbjct: 14 KKVVLAYSGGLDTSIILRWLQDEYDCEVVTFTADIGQGEELEPARQKAEMMGIKEIYIED 73 Query: 64 LRDEFASDFIYPMFQSGALYEGQYLLGTSIARPLIAKRMVDIAIAEGATAIAHGATGKGN 123 LR+EF D+++PMF++ LYEG YLLGTSIARPLI KR+V+IA A GA A++HGATGKGN Sbjct: 74 LREEFVRDYVFPMFRANTLYEGVYLLGTSIARPLIGKRLVEIAEATGADAVSHGATGKGN 133 Query: 124 DQVRFELNAAALSPSIKVIAPWRLEEFRSQFPGRAEMIAYAEANGIPV--QASAAKPYSM 181 DQVRFEL A AL P IK+IAPWR + S R ++I YA + IPV PYSM Sbjct: 134 DQVRFELTAYALKPDIKIIAPWRTWDLHS----RTKLIEYAMRHQIPVPKDKHGEAPYSM 189 Query: 182 DRNLLHISYESGVLEDPWFDPSAPENKGMFLLSNAPEDAPDQPEYLELEFLKGDCVALNG 241 D NLLHISYE LE+PW +PS + MF L+ +PE APD+ +Y+E++F +GD VA++G Sbjct: 190 DANLLHISYEGKALENPWTEPS----EDMFRLTVSPEAAPDKAQYIEVDFERGDAVAIDG 245 Query: 242 EPLSPLQVMEKLNELGGKHGIGRVDMVENRFVGMKSRGVYETPGGTILFTAHRKMESITM 301 E L+P ++ KLNE+GG+HG+GR+D+VENR+VGMKSRGVYETPGGTIL AHR +ES+T+ Sbjct: 246 EKLTPAALLAKLNEIGGRHGVGRLDLVENRYVGMKSRGVYETPGGTILQVAHRAVESLTL 305 Query: 302 DREVMNLRDSLITRYSTLVYNGFWFAPERLALQALVKESQKNVTGTVRVKLYKGNIIGAG 361 DREVM+LRD L+ RY+ L+YNGFWFAPERL LQA + ++Q+ VTGT R+KLYKGN+ G Sbjct: 306 DREVMHLRDELMPRYAKLIYNGFWFAPERLMLQAAIDQTQQTVTGTARLKLYKGNVSVVG 365 Query: 362 VKSPVSLYNPEIATMEADPTQAYDQGDATGFIRLNALRLKV 402 K+ SLY + T E D YDQ DA GFI+LNALRL++ Sbjct: 366 RKAAKSLYRMDYVTFEEDTV--YDQHDAEGFIKLNALRLRL 404 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 611,073,336 Number of extensions: 26112802 Number of successful extensions: 61201 Number of sequences better than 1.0e-05: 793 Number of HSP's gapped: 59754 Number of HSP's successfully gapped: 793 Length of query: 411 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 283 Effective length of database: 409,704,906 Effective search space: 115946488398 Effective search space used: 115946488398 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)