BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0007.p (385 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00086793.1| COG1454: Alcohol dehydrogenase, class IV [Pse... 234 4e-60 gb|EAJ64171.1| unknown [environmental sequence] 225 1e-57 gb|AAC98741.1| alcohol dehydrogenase [Pseudomonas putida] 218 1e-55 gb|EAI16794.1| unknown [environmental sequence] 218 2e-55 sp|P06758|ADH2_ZYMMO Alcohol dehydrogenase II (ADH II) >gi|78223... 215 2e-54 >ref|ZP_00086793.1| COG1454: Alcohol dehydrogenase, class IV [Pseudomonas fluorescens PfO-1] Length = 405 Score = 234 bits (596), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 135/376 (35%), Positives = 215/376 (57%), Gaps = 4/376 (1%) Query: 6 FQTARSIIAGCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAVHW 65 F+ A +I G G++E++ L +L ++ L+V+ L SGT++ L + G Sbjct: 29 FKIAHKLITGAGAIEQLAAELTRL-DIDNPLIVTDAALVKSGTVE--LALAQIGEREYEI 85 Query: 66 NTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLGTD 125 V P+P IA +E+ E D +IG+GGGS +D+ K ++ ++ ++ G D Sbjct: 86 FDRVLPDPEIAIVEDCMRVYREGGHDGLIGLGGGSAIDIAKSVAAYAGYHGALEDLFGVD 145 Query: 126 LIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXGLP 185 +P G P + IPTT+GTGSEVT AI++ +LK G+VS Y P Sbjct: 146 QVPRKGPPLIAIPTTAGTGSEVTNVAILSDKVAQLKKGIVSDYLLPDVALVSPQMTLTCP 205 Query: 186 RDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAREDML 245 R +TA++G+DA HA+ES++S A+ +D A+ +I+ I+ S+ +AY + ++ +AREDM Sbjct: 206 RSVTAASGVDALVHAIESYLSLNASPITDSLAIGAIKLIARSLPKAYANPSNLQAREDMA 265 Query: 246 IGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLALVA 305 S+ GMA AG AVHALAYP+GG+F++ HGV+N++LLP+VM +N VER+ +A Sbjct: 266 TASLMAGMAFGNAGVGAVHALAYPLGGRFNIAHGVSNALLLPYVMTWNKMACVERMQDIA 325 Query: 306 GAMGLQTG-AGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQVTRLM 364 AMG++T A+ + + + IP L FG E IP +++ A+ + RLM Sbjct: 326 EAMGVKTAHLSLNEAADSAVAAMTELCAAVEIPLGLRSFGVPEEAIPAMAVEAAGIERLM 385 Query: 365 GNNPKAMTLDDIGAVY 380 NNP+ ++ DI +Y Sbjct: 386 RNNPRKLSAADIEKIY 401 >gb|EAJ64171.1| unknown [environmental sequence] Length = 370 Score = 225 bits (574), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 131/367 (35%), Positives = 208/367 (56%), Gaps = 4/367 (1%) Query: 15 GCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAVHWNTDVQPEPT 74 G G++E++ L +L ++ L+V+ L SGT+ L G A V P+P Sbjct: 3 GAGAIEQLAAELTRL-DVDNPLIVTDAALVQSGTV--ALALEHLGDRAYEIFDRVLPDPE 59 Query: 75 IAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLGTDLIPNPGIPT 134 IA +E+ A E D +IG+GGGS +D+ K ++ ++ ++ G D +P G P Sbjct: 60 IAIVEDCMRAYREGGHDGLIGVGGGSAIDIAKSVAAYAGYHGALADLFGVDQVPRKGPPL 119 Query: 135 VLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXGLPRDITASTGM 194 + IPTT+GTGSEVT AI++ +LK G+VS Y PR +TA++G+ Sbjct: 120 IAIPTTAGTGSEVTNVAILSDKAAQLKKGIVSDYLLPDVALISPQMTLTCPRGVTAASGV 179 Query: 195 DAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAREDMLIGSMYGGMA 254 DA HA+ES++S A+ +D A+ +I+ I++++ +AY + + +AR+DM S+ GMA Sbjct: 180 DALVHAIESYLSLNASPITDALAIGAIKLIASALPKAYANPANLQARDDMATASLMAGMA 239 Query: 255 LTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLALVAGAMGLQTGA 314 AG AVHALAYP+GG+F++ HGV+N++LLP+VM +N VER+ +A AMG+ Sbjct: 240 FGNAGVGAVHALAYPLGGRFNIAHGVSNALLLPYVMHWNKLACVERMQDIAQAMGVNIAG 299 Query: 315 GRQRD-AEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQVTRLMGNNPKAMTL 373 D A+ + + + IP L FG + IP ++ A+ + RLM NNP+ ++ Sbjct: 300 LSVNDAADQAVAAMTRLCAAVEIPSGLRSFGVPEDAIPAMASEAAGIERLMRNNPRKLSA 359 Query: 374 DDIGAVY 380 DI +Y Sbjct: 360 ADIEKIY 366 >gb|AAC98741.1| alcohol dehydrogenase [Pseudomonas putida] Length = 382 Score = 218 bits (556), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 130/381 (34%), Positives = 207/381 (54%), Gaps = 4/381 (1%) Query: 1 MEQYFFQTARSIIAGCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGG 60 M FQ A ++ G ++E++ L +L + L+V+ L SGT+D L GG Sbjct: 1 MSVSAFQIANKLLTGASAVEQLDAELTRL-KVSNPLIVTDAILLKSGTVD--LALAHLGG 57 Query: 61 IAVHWNTDVQPEPTIAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVRE 120 + QPEP I+ +E+ A E D +IG+GGGS +D+ K ++ + ++ + Sbjct: 58 RSYGLYDQAQPEPEISLVEDCTRAFREGGHDGLIGLGGGSAIDIAKGVAAFAGHDGALAD 117 Query: 121 MLGTDLIPNPGIPTVLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXX 180 + G D + G P + IPTT+GTGSEV + + +VS Y Sbjct: 118 LFGVDQVSRKGPPLIAIPTTAGTGSEVLTWRSFPTRKRSSRKALVSDYLLPDVALVSPGL 177 Query: 181 XXGLPRDITASTGMDAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKA 240 LP +++ ++G+DA HA+E+++S + +D AL +I+ I+ ++ +AY + +D A Sbjct: 178 DAHLPTNVSRASGVDALVHAIEAYLSINRSPITDAIALGAIKLIARALPKAYANPDDLAA 237 Query: 241 REDMLIGSMYGGMALTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVER 300 REDM S+ GGMA AG AVHALAYP+GG+F++ HGV+N++LLPHVM +N ER Sbjct: 238 REDMATASLMGGMAFGNAGVGAVHALAYPLGGRFNIAHGVSNALLLPHVMRWNKLACTER 297 Query: 301 LALVAGAMGLQ-TGAGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELSLAASQ 359 L VA AMG+ G + A+ ++ + + IP L F ++ + ++ AS+ Sbjct: 298 LRDVAQAMGVNIAGLSDSQAADASVDAMAELCAAVDIPTGLRAFNVPQDALAPMAEEASK 357 Query: 360 VTRLMGNNPKAMTLDDIGAVY 380 + RLM NNP+ T DI A+Y Sbjct: 358 IDRLMRNNPRKFTAGDIEAIY 378 >gb|EAI16794.1| unknown [environmental sequence] Length = 302 Score = 218 bits (555), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 115/291 (39%), Positives = 179/291 (61%), Gaps = 1/291 (0%) Query: 91 DAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLGTDLIPNPGIPTVLIPTTSGTGSEVTPN 150 D +IG+GGGS +D+ K ++ + ++ E+ G D I G P + IPTT+GTGSEVT Sbjct: 8 DGLIGVGGGSAIDIAKAVAGYVGHDGALEELFGVDQIKRKGPPLIAIPTTAGTGSEVTNV 67 Query: 151 AIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXGLPRDITASTGMDAYTHALESFISNKAN 210 +I++ + +LK G+VS Y PR +TA++G+DA HA+ES++S A+ Sbjct: 68 SILSDKKAQLKKGIVSDYLLPDVALVSPLMTLTCPRGVTAASGVDALVHAVESYLSVNAS 127 Query: 211 RFSDMFALESIRNISASMLQAYHHGNDRKAREDMLIGSMYGGMALTCAGTAAVHALAYPI 270 +D A+ +I+ I+ ++ +AY + D AREDM S+ GMA AG AVHALAYP+ Sbjct: 128 PITDALAIGAIKLIAKALPKAYANPADLVAREDMATASLMAGMAFGNAGVGAVHALAYPL 187 Query: 271 GGKFHVPHGVANSMLLPHVMEFNFDVIVERLALVAGAMGLQT-GAGRQRDAEHVIEQIRL 329 GG+F++ HGV+N++LLP+VM +N VER+A +A A+G+QT G + A+ +E + Sbjct: 188 GGRFNIAHGVSNALLLPYVMAWNKLACVERMAEIAAALGVQTHGLSDKAAADLAVEAMAE 247 Query: 330 WTREMSIPQDLTLFGAAREDIPELSLAASQVTRLMGNNPKAMTLDDIGAVY 380 + IP+ + FG + IP ++ AS++ RLM NNP+ +T DI +Y Sbjct: 248 LCASVDIPKGMRSFGVPEDAIPAMAEEASKIDRLMRNNPRRLTAGDIEQIY 298 >sp|P06758|ADH2_ZYMMO Alcohol dehydrogenase II (ADH II) pir||A25978 alcohol dehydrogenase (EC 1.1.1.1) 2 - Zymomonas mobilis gb|AAA27683.1| alcohol dehydrogenase II (EC 1.1.1.1) Length = 383 Score = 215 bits (547), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 123/341 (36%), Positives = 187/341 (54%), Gaps = 2/341 (0%) Query: 15 GCGSLERIVEHLQKLGSFESVLLVSQPFLQSSGTLDEVTNLLKSGGIAVHWNTDVQPEPT 74 G GSLE+ ++ L G F++ L+VS F+ SG + +V +LLK+ GI V P PT Sbjct: 15 GEGSLEKAIKDLNGSG-FKNALIVSDAFMNKSGVVKQVADLLKAQGINSAVYDGVMPNPT 73 Query: 75 IAQLEELYSAIAEIPCDAIIGIGGGSVLDVVKLLSVMKTNSLSVREMLGTDLIPNPGIPT 134 + + E + + D +I +GGGS D K ++++ TN V++ G D P +P Sbjct: 74 VTAVLEGLKILKDNNSDFVISLGGGSPHDCAKAIALVATNGGEVKDYEGIDKSKKPALPL 133 Query: 135 VLIPTTSGTGSEVTPNAIVTLPEEELKIGVVSRYXXXXXXXXXXXXXXGLPRDITASTGM 194 + I TT+GT SE+T I+T +K+ +V R+ G+P+ +TA+TGM Sbjct: 134 MSINTTAGTASEMTRFCIITDEVRHVKMAIVDRHVTPMVSVNDPLLMVGMPKGLTAATGM 193 Query: 195 DAYTHALESFISNKANRFSDMFALESIRNISASMLQAYHHGNDRKAREDMLIGSMYGGMA 254 DA THA E++ S A +D AL++ I+ ++ A +G D ARE M GMA Sbjct: 194 DALTHAFEAYSSTAATPITDACALKAASMIAKNLKTACDNGKDMPAREAMAYAQFLAGMA 253 Query: 255 LTCAGTAAVHALAYPIGGKFHVPHGVANSMLLPHVMEFNFDVIVERLALVAGAMGLQ-TG 313 A VHA+A+ +GG +++PHGV N++LLPHV+ +N V+ RL V AMGL Sbjct: 254 FNNASLGYVHAMAHQLGGYYNLPHGVCNAVLLPHVLAYNASVVAGRLKDVGVAMGLDIAN 313 Query: 314 AGRQRDAEHVIEQIRLWTREMSIPQDLTLFGAAREDIPELS 354 G + AE I+ +R + IP +LT GA +ED+P L+ Sbjct: 314 LGDKEGAEATIQAVRDLAASIGIPANLTELGAKKEDVPLLA 354 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 522,053,102 Number of extensions: 20604462 Number of successful extensions: 56788 Number of sequences better than 1.0e-05: 934 Number of HSP's gapped: 54992 Number of HSP's successfully gapped: 934 Length of query: 385 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 258 Effective length of database: 412,420,005 Effective search space: 106404361290 Effective search space used: 106404361290 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)