BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0514.0002.p (1061 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_653904.1| ACR_tran, AcrB/AcrD/AcrF family [Bacillus anthr... 356 2e-96 ref|ZP_00098314.1| COG0841: Cation/multidrug efflux pump [Desulf... 355 4e-96 ref|NP_981860.1| transporter, AcrB/AcrD/AcrF family [Bacillus ce... 352 3e-95 ref|NP_835096.1| Acriflavin resistance plasma membrane protein [... 351 5e-95 ref|NP_347010.1| Cation efflux system protein, AcrB/AcrF/AcrD fa... 343 9e-93 >ref|NP_653904.1| ACR_tran, AcrB/AcrD/AcrF family [Bacillus anthracis A2012] ref|NP_847832.1| transporter, AcrB/AcrD/AcrF family [Bacillus anthracis str. Ames] gb|AAP29318.1| transporter, AcrB/AcrD/AcrF family [Bacillus anthracis str. Ames] Length = 1038 Score = 356 bits (913), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 299/1070 (27%), Positives = 528/1070 (49%), Gaps = 115/1070 (10%) Query: 35 ILTIVVVLLMAWGAVSAFQMQRDYLPGINNTTLMVSLRASAYQADQVKRDITTPIEQAIR 94 +LTI+V + G S M+ + +P I + V+ ++V ++ P+E+ ++ Sbjct: 17 LLTIIVTI---AGIYSGLNMKLETIPDITTPVVTVTTVYPGATPEEVADKVSKPMEEQLQ 73 Query: 95 KTNGILNVETTSYDGGLLMNLYYPMNYNMDKAEQDIRMALGDADLPANVNAPTVTRLTTS 154 +G+ V ++S+ + + Y + NM+KAE +I+ AL + LP V P V+R+ + Sbjct: 74 NLSGVNVVSSSSFQNASSIQVEYDFDKNMEKAETEIKDALANVKLPEGVKDPKVSRVNFN 133 Query: 155 TFPVLSYSLTANNEKVDDDTLRSTVQAGIVNQLKSVPGVSDVQAVGGAKNGYVVLIHTKD 214 FPV+S S+ + NE + TL V+ +V LK + GV+ VQ + G + V L+ KD Sbjct: 134 AFPVISLSVASKNESL--ATLTENVEKNVVPGLKGLDGVASVQ-ISGQQVDEVQLVFKKD 190 Query: 215 -LSAAGLTLDDFN---KSVSAAVP-NLQGYIQNVKSPFPVRVEGWDMTEQDVNNIVI--- 266 + GL+ D K ++P L + KS V V+G T + + + I Sbjct: 191 KMKELGLSEDTVKNVIKGSDVSLPLGLYTFKDTEKS---VVVDGNITTMKALKELKIPAV 247 Query: 267 ---KNSDGV---------------------SVPLSAIASVSRSLNDVQTMSRTDGKASVL 302 +S G +V LS IA + + + +++SRT+GK ++ Sbjct: 248 PSSASSQGSQTAGAGAQMPQMNPAAMNGIPTVTLSEIADI-KEVGKAESISRTNGKEAIG 306 Query: 303 INVIKTSGANITDVAKQVKTRVAALPAVEGGNVTMTLTGDRAHDLNASLLGLVREGLLGC 362 I ++K + AN DV VK +V L + ++ + T D+ + S+ ++ + + G Sbjct: 307 IQIVKAADANTVDVVNAVKDKVKELEK-KYKDLEIISTFDQGAPIEKSVETMLSKAIFGA 365 Query: 363 VFSMLCVLFFFRNVRSTLLIAVSLPISLLATTAILKAMGVTLNILTVSGLIVAMGRIVDD 422 +F+++ ++ F RN+R+TL+ VS+P+SLL ++K M +TLNI+T+ + VA+GR+VDD Sbjct: 366 IFAIVIIMLFLRNIRTTLISVVSIPLSLLIAVLVIKQMDITLNIMTLGAMTVAIGRVVDD 425 Query: 423 AIVILDNMHRKM--QEKKDQGALHVLAESVVEMLPAIFGSTATTVAVYAPIALVGGIIGA 480 +IV+++N++R+M E+K +G ++ E+ EM I ST T+AV+ P+ LV G+IG Sbjct: 426 SIVVIENIYRRMSLSEEKLRGK-DLIREATKEMFIPIMSSTIVTIAVFLPLGLVKGMIGE 484 Query: 481 SYSGFAWSVVIALAVSFLVAMLVIPAFAYMGWHKPGGKAVTLEPAMKP---------FLT 531 + FA ++V AL S LVA+ ++P A+ + K + + KP L Sbjct: 485 MFLPFALTIVFALLASLLVAVTIVPMLAHSLFKKESMREKEVHHEEKPSKLANIYKRILA 544 Query: 532 TALKHKKTVLGVSLVIFIAAAFFASQLPFSLLPSTASGQVAVQLELPKGTALSEVDKAVQ 591 AL HK +++++ + + + S LPS + G L +V+K Sbjct: 545 WALNHKIITSSIAVLLLVGSLALVPIIGVSFLPSEEEKMIIATYNPEPGQTLEDVEKIAT 604 Query: 592 NVETVLKQDSQVAXXXXXXXXXXXPQADDVFDQGGGYIQQPN-IANLSVALKDQKQVNSY 650 E QD++ P + +Q ++Q N N K+++QV Sbjct: 605 KAEKHF-QDNKDVKTIQFSLGGENPMSPGQSNQAMFFVQYDNDTKNFE---KEKEQV--- 657 Query: 651 INSLQERLGNQQGVAAVTITNQNI--AGDDSTINIMLSGSDAGTLDKAASTVRSGLAGIE 708 + LQ+ G + NQ+ +G + I + + G + + +++ + + Sbjct: 658 VKDLQKMSGKGEW------KNQDFGASGGSNEIKLYVYGDSSEDIKPVVKDIQNIMKKNK 711 Query: 709 GLSVVGKTDLTNGIPK----FSIALDRGKVLKAGVSRNDI---------NKVLSRYMTKG 755 L D+ + I K +++ D+ K+ K G++ I VL+ G Sbjct: 712 DLK-----DIDSSIAKTYAEYTLVADQEKLSKMGLTAAQIGMGLSNQHDRPVLTTIKKDG 766 Query: 756 KDFDISVSSGTGIIPVDVYLDPVKMGQAQAGGPLPVYTPDQVLASLAAETVKGKDGTVYR 815 KD V+VY++ + Q + T ++ L E V KD Sbjct: 767 KD-------------VNVYVE----AEKQTYETIDDLTNRKITTPLGNE-VAVKD----- 803 Query: 816 LDQLGTLQRSDAGSSIQERDGQPYTVVQARIVSSDTSGVSKAVNKVLRTTNLPEGVTYSL 875 + T++ + ++++ RDG+ Y V A++ S D S S AV K + +LP GV S+ Sbjct: 804 ---VMTVKEGETSNTVKHRDGRVYAEVSAKLTSDDVSKASAAVQKEVDKMDLPSGVDVSM 860 Query: 876 GGITQQVTQMIFEMIIAVVLSIFLVLLITSFVFRSWRAPIAVLISIPLALSGVVLALYTV 935 GG+T+ + + ++ +A++ +I +V + F AP A+L S+P + G ++AL Sbjct: 861 GGVTKDIEESFKQLGLAMLAAIAIVYFVLVVTFGGALAPFAILFSLPFTIIGALVALLIS 920 Query: 936 HGQWNLAALIGVLMLTGIVVTNGIVLIDKIERNRREGLGLRDAVMQGSLSRVRPIFMTAA 995 +++A+IG LML GIVVTN IVLID++ EGL R+A+++ +R+RPI MTA Sbjct: 921 GETLSVSAMIGALMLIGIVVTNAIVLIDRVIHKENEGLSTREALLEAGATRLRPILMTAI 980 Query: 996 TTILTLVPLTLSGDDGTVISKVLGVVVIGGMITSTLNSFVVIPILYEAMN 1045 TI L+PL L + +ISK LGV VIGG+ +STL + +++PI+YE ++ Sbjct: 981 ATIGALIPLALGFEGSGLISKGLGVTVIGGLTSSTLLTLLIVPIVYEVLS 1030 >ref|ZP_00098314.1| COG0841: Cation/multidrug efflux pump [Desulfitobacterium hafniense] Length = 1028 Score = 355 bits (910), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 292/1069 (27%), Positives = 514/1069 (48%), Gaps = 89/1069 (8%) Query: 36 LTIVVVLLMAWGAVSAFQMQRDYLPGINNTTLMVSLRASAYQADQVKRDITTPIEQAIRK 95 +T+++V+L+ G VS ++ D P +V S ++++ IT PIE A+ Sbjct: 1 MTVLIVILL--GTVSLTRLPIDLFPEFELPMAIVMTEYSGVGPQEIEKLITNPIEGAVSS 58 Query: 96 TNGILNVETTSYDGGLLMNLYYPMNYNMDKAEQDIRMALG--DADLPANVNAPTVTRLTT 153 T I V +T+ +G ++ + +M+ A +R + LP +P V ++ Sbjct: 59 TENIDIVTSTTTEGTSIVMASFKTGTDMNFATLQMREKVDMIKFALPEGAGSPMVLKIDI 118 Query: 154 STFPVLSYSLTANNEKVDDDTLRSTVQAGIVNQLKSVPGVSDVQAVGGAKNGYVVLIHTK 213 S P++ ++++ N VD L++ + I +++ V GV+ V GG +N + H + Sbjct: 119 SMMPIMQLTMSSAN--VDLAQLQALAEDEIKPRMERVKGVASVSISGGYENEVKIKTHQE 176 Query: 214 DLSAAGLTLDDFNKSVSAAVPNLQ---GYIQNVKSPFPVRVEGWDMTEQDVNNIVIKNSD 270 + GL++ + ++A NL G ++ +R G T +++ N++I + Sbjct: 177 KMQGYGLSISTLSGILAAE--NLNSPAGEVKKGNQDLTIRTTGEFQTLEEIENLLIPLNT 234 Query: 271 GVSVPLSAIASVSRSLNDVQTMSRTDGKASVLINVIKTSGANITDVAKQVKTRVAALPAV 330 G V L IA V DV T+SRT+G SV I + K SG N VA+ + + L A Sbjct: 235 GGQVRLKDIADVELGHTDVTTISRTNGLQSVSIAIQKQSGVNTVAVAEDITKAIGELKA- 293 Query: 331 EGGNVTMTLTGDRAHDLNASLLGLVREGLLGCVFSMLCVLFFFRNVRSTLLIAVSLPISL 390 E +T+ D + + ++ + +E LL + ++ + FF N+R+T + A ++PI++ Sbjct: 294 EFPELTLDTVYDESVYIKETINTVFKEALLAGILAIGILFVFFHNLRTTFITATAIPIAV 353 Query: 391 LATTAILKAMGVTLNILTVSGLIVAMGRIVDDAIVILDNMHRKMQEKKDQGALHVLAESV 450 +AT A L M V++N++T+ GL +AMGR+VDD IV L+N++R + + + V Sbjct: 354 MATFACLYFMDVSINMMTLGGLSLAMGRLVDDNIVALENIYRFREAGHSK--FDAAIKGV 411 Query: 451 VEMLPAIFGSTATTVAVYAPIALVGGIIGASYSGFAWSVVIALAVSFLVAMLVIPAFA-- 508 E+ AI ST TTVAV+ PI V G+ + A +V I+L S LV++ ++PA + Sbjct: 412 SEVGMAITASTLTTVAVFLPIVFVEGLTATLFRDLALTVGISLGASLLVSLTLVPALSAK 471 Query: 509 --YMGWHKPGGKAV----------------TLEPAMKPFLTTALKHKKTVLGVSLVIFIA 550 +G G K + +E + + FL AL H+KT++ S++IF+ Sbjct: 472 IMKVGEIPVGRKGLRGLFDGFGRGFDNGFGRIEKSYRRFLHYALGHRKTIVVGSILIFVL 531 Query: 551 AAFFASQLPFSLLPSTASGQVAVQLELPKGTALSEVDKAVQNVETVLKQDSQVAXXXXXX 610 + + +PS+ +GQ+ V ++LP G L + D A+ VE L+ +V Sbjct: 532 SGATTLFVGAEFMPSSDAGQMTVSVKLPDGAQLEDTDAAIAEVEKHLEGIEEVQTAFAQV 591 Query: 611 XXXXXPQADDVFDQGGGYIQQPNIANLSVALKDQKQVNSYINSLQERLGNQQGVA-AVTI 669 G Q L ++ ++ V+ L+ + G V + Sbjct: 592 GGGGLMSFGGASGNSGSITIQ-----LVPLMERERSVDEVAEELRALTKDIPGAKIEVAV 646 Query: 670 TNQNIAGDDSTINIMLSGSDAGTLDKAASTVRSGLAGIEGLSVVGKTDLTNGIPKFSIAL 729 T+ G + I+I + G + L + A+ +++ +A +EG V KT + GIP+ +A+ Sbjct: 647 TDMMSMGTTTPIDIAIKGDELDQLQQIATDIQAMVASVEGTREV-KTSMGEGIPEVQVAI 705 Query: 730 DRGKVLKAGVSRNDINKVL---------SRYMTKGKDFDISVSSGTGIIPVDVYLDPVKM 780 DR K K G++ I L +RY +GK+ ++ ++ D K Sbjct: 706 DREKASKYGLTAYQIANGLKGTLSGTTATRYRYEGKEINVVITGD----------DTFK- 754 Query: 781 GQAQAGGPLPVYTPDQVLASLAAETVKGKDGTVYRLDQLG--TLQRSDAGSSIQERDGQP 838 Q L++L + GT L Q+ T++R G + ER GQ Sbjct: 755 ---------------QSLSNLEQTPITTPLGTDVPLSQIAEVTIER---GPTAIERSGQA 796 Query: 839 YTV-VQARIVSSDTSGVSKAVNKVLRTTNLPEGVTYSLGGITQQVTQMIFEMIIAVVLSI 897 V V + IV D V+ + L LP G TY +GG +++ + ++ +A++L+I Sbjct: 797 RVVHVSSDIVGRDLVSVTNDIQAKLNNYPLPNGYTYDMGGENEEMVKSFQDLGMALILAI 856 Query: 898 FLVLLITSFVFRSWRAPIAVLISIPLALSGVVLALYTVHGQWNLAALIGVLMLTGIVVTN 957 LV +I + F S P ++ S+P+ SG +L L+ ++ A IG+++LTGIVV+N Sbjct: 857 VLVYMILAAQFESLIHPFTIIFSLPMGFSGGMLGLFITRSTISVPAFIGLILLTGIVVSN 916 Query: 958 GIVLIDKIERNRREGLGLRDAVMQGSLSRVRPIFMTAATTILTLVPLTLSGDDGTVISKV 1017 IVL+D I + R G G +A+ R+RPI MT+ T L L+P+ + +G Sbjct: 917 AIVLVDYINKRREMGDGREEAIENAGPIRLRPIMMTSLATALGLIPMAVGIGEGAETMAP 976 Query: 1018 LGVVVIGGMITSTLNSFVVIPILY-------EAMNRKKFREASLTGTST 1059 + +VVI GM STL++ +++P++Y + RK R+A+ + T Sbjct: 977 MAIVVIFGMTLSTLSTLILVPVIYTINEDFKNFLKRKGKRKAASESSET 1025 >ref|NP_981860.1| transporter, AcrB/AcrD/AcrF family [Bacillus cereus ATCC 10987] gb|AAS44468.1| transporter, AcrB/AcrD/AcrF family [Bacillus cereus ATCC 10987] Length = 1039 Score = 352 bits (903), Expect = 3e-95, Method: Compositional matrix adjust. Identities = 290/1067 (27%), Positives = 524/1067 (49%), Gaps = 108/1067 (10%) Query: 35 ILTIVVVLLMAWGAVSAFQMQRDYLPGINNTTLMVSLRASAYQADQVKRDITTPIEQAIR 94 +LTI+V + G S M+ + +P I + V+ ++V ++ P+E+ ++ Sbjct: 17 LLTIIVTI---AGIYSGLNMKLETIPDITTPVVTVTTVYPGATPEEVADKVSKPMEEQLQ 73 Query: 95 KTNGILNVETTSYDGGLLMNLYYPMNYNMDKAEQDIRMALGDADLPANVNAPTVTRLTTS 154 +G+ V ++S+ + + Y + NM+KAE +I+ AL + LP V P V+R+ + Sbjct: 74 NLSGVNVVSSSSFQNASSIQVEYDFDKNMEKAETEIKEALANVKLPEGVKDPKVSRVNFN 133 Query: 155 TFPVLSYSLTANNEKVDDDTLRSTVQAGIVNQLKSVPGVSDVQAVGGAKNGYVVLIHTKD 214 FPV+S S+ + NE + TL V+ +V LK + GV+ VQ + G + V L+ KD Sbjct: 134 AFPVISLSVASKNESL--ATLTENVEKNVVPGLKGLDGVASVQ-ISGQQVDEVQLVFKKD 190 Query: 215 -LSAAGLTLDDFN---KSVSAAVP-NLQGYIQNVKSPFPVRVEGWDMTEQDVNNIVI--- 266 + GL+ D K ++P L + KS V V+G T + + + I Sbjct: 191 KMKELGLSEDTVKNVIKGSDVSLPLGLYTFKDTEKS---VVVDGNITTMKALKELKIPAV 247 Query: 267 ---------KNS---------------DGVS-VPLSAIASVSRSLNDVQTMSRTDGKASV 301 +N+ +G+S V L IA + + + +++SRT+GK ++ Sbjct: 248 PSSASGQGSQNAGAGTQVPQMNPAAMNNGISTVTLDEIADI-KEVGKAESISRTNGKEAI 306 Query: 302 LINVIKTSGANITDVAKQVKTRVAALPAVEGGNVTMTLTGDRAHDLNASLLGLVREGLLG 361 I ++K + AN DV VK +V L + ++ + T D+ + S+ ++ + + G Sbjct: 307 GIQIVKAADANTVDVVNAVKDKVKELEK-KYKDLEIISTFDQGAPIEKSVETMLSKAIFG 365 Query: 362 CVFSMLCVLFFFRNVRSTLLIAVSLPISLLATTAILKAMGVTLNILTVSGLIVAMGRIVD 421 +F+++ ++ F RN+R+TL+ VS+P+SLL ++K M +TLNI+T+ + VA+GR+VD Sbjct: 366 AIFAIVIIMLFLRNIRTTLISVVSIPLSLLIAVLVIKQMDITLNIMTLGAMTVAIGRVVD 425 Query: 422 DAIVILDNMHRKM--QEKKDQGALHVLAESVVEMLPAIFGSTATTVAVYAPIALVGGIIG 479 D+IV+++N++R+M +K +G ++ E+ EM I ST T+AV+ P+ LV G+IG Sbjct: 426 DSIVVIENIYRRMSLSGEKLRGK-DLIREATKEMFIPIMSSTIVTIAVFLPLGLVKGMIG 484 Query: 480 ASYSGFAWSVVIALAVSFLVAMLVIPAFAYMGWHKPGGKAVTLEPAMKP---------FL 530 + FA ++V AL S LVA+ ++P A+ + K + + KP L Sbjct: 485 EMFLPFALTIVFALLASLLVAVTIVPMLAHSLFKKESMREKEVHHEEKPSKLANIYKHIL 544 Query: 531 TTALKHKKTVLGVSLVIFIAAAFFASQLPFSLLPSTASGQVAVQLELPKGTALSEVDKAV 590 AL HK +++++ + + + S LPS + G L +V+K Sbjct: 545 AWALNHKIITSSIAILLLVGSLALVPIIGVSFLPSEEEKMIIATYNPEPGQTLEDVEKIA 604 Query: 591 QNVETVLKQDSQVAXXXXXXXXXXXPQADDVFDQGGGYIQQPN-IANLSVALKDQKQVNS 649 E QD++ P + +Q ++Q N N K+++QV Sbjct: 605 TKAEKHF-QDNKNVKTIQFSLGGENPMSPGQSNQAMFFVQYDNDTKNFE---KEKEQV-- 658 Query: 650 YINSLQERLGNQQGVAAVTITNQNI--AGDDSTINIMLSGSDAGTLDKAASTVRSGLAGI 707 + LQ+ G + NQ+ +G + I + + G + + +++ + Sbjct: 659 -VKDLQKMSGKGEW------KNQDFGASGGSNEIKLYVYGDSSEDIKPVVKDIQNIMKKN 711 Query: 708 EGLSVVGKTDLTNGIPKFSIALDRGKVLKAGVSRNDI---------NKVLSRYMTKGKDF 758 + L + + + ++++ D+ K+ K G++ I VL+ GKD Sbjct: 712 KDLKDI-DSSIAKTYAEYTLVADQEKLSKMGLTAAQIGMGLSNQHDRPVLTTIKKDGKD- 769 Query: 759 DISVSSGTGIIPVDVYLDPVKMGQAQAGGPLPVYTPDQVLASLAAETVKGKDGTVYRLDQ 818 ++VY++ K + + L + G + Sbjct: 770 ------------INVYVEAEKQNY-------------ETIDDLTNRKITTPLGNEVAVKD 804 Query: 819 LGTLQRSDAGSSIQERDGQPYTVVQARIVSSDTSGVSKAVNKVLRTTNLPEGVTYSLGGI 878 + T++ + ++++ RDG+ Y V A++ S D S S AV K + +LP GV S+GG+ Sbjct: 805 VMTVKEGETSNTVKHRDGRVYAEVSAKLTSDDVSKASAAVQKEVDKMDLPSGVDVSMGGV 864 Query: 879 TQQVTQMIFEMIIAVVLSIFLVLLITSFVFRSWRAPIAVLISIPLALSGVVLALYTVHGQ 938 T+ + + ++ +A++ +I +V + F AP A+L S+P + G ++AL Sbjct: 865 TKDIEESFKQLGLAMLAAIAIVYFVLVVTFGGALAPFAILFSLPFTIIGALVALLISGET 924 Query: 939 WNLAALIGVLMLTGIVVTNGIVLIDKIERNRREGLGLRDAVMQGSLSRVRPIFMTAATTI 998 +++A+IG LML GIVVTN IVLID++ EGL R+A+++ +R+RPI MTA TI Sbjct: 925 LSVSAMIGALMLIGIVVTNAIVLIDRVIHKENEGLSTREALLEAGATRLRPILMTAIATI 984 Query: 999 LTLVPLTLSGDDGTVISKVLGVVVIGGMITSTLNSFVVIPILYEAMN 1045 L+PL L + +ISK LGV VIGG+ +STL + +++PI+YE ++ Sbjct: 985 GALIPLALGFEGSGLISKGLGVTVIGGLTSSTLLTLLIVPIVYEVLS 1031 >ref|NP_835096.1| Acriflavin resistance plasma membrane protein [Bacillus cereus ATCC 14579] gb|AAP12297.1| Acriflavin resistance plasma membrane protein [Bacillus cereus ATCC 14579] Length = 1038 Score = 351 bits (901), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 287/1074 (26%), Positives = 517/1074 (48%), Gaps = 123/1074 (11%) Query: 35 ILTIVVVLLMAWGAVSAFQMQRDYLPGINNTTLMVSLRASAYQADQVKRDITTPIEQAIR 94 +LTI+V + G S M+ + +P I + V+ ++V ++ P+E+ ++ Sbjct: 17 LLTIIVTI---AGIYSGLNMKLETIPDITTPIVTVTTVYPGATPEEVADKVSKPMEEQLQ 73 Query: 95 KTNGILNVETTSYDGGLLMNLYYPMNYNMDKAEQDIRMALGDADLPANVNAPTVTRLTTS 154 +G+ V ++S+ + + Y + NM+KAE +I+ AL + LP V P V+R+ + Sbjct: 74 NLSGVNVVSSSSFQNASSIQVEYDFDKNMEKAETEIKEALTNVKLPEGVKDPKVSRVNFN 133 Query: 155 TFPVLSYSLTANNEKVDDDTLRSTVQAGIVNQLKSVPGVSDVQAVGGAKNGYVVLIHTKD 214 FPV+S S+ + NE + TL V+ +V LK + GV+ VQ + G + V L+ KD Sbjct: 134 AFPVISLSVASKNESLA--TLTENVEKNVVPGLKGLDGVASVQ-IAGQQVDEVQLVFKKD 190 Query: 215 -LSAAGLTLDDFNKSVSAAVPNLQGYIQNVKSPFPVRVEGWDMTEQDV----NNIVIKNS 269 + GL+ D ++ I+ P+ + + TE+ V N IK Sbjct: 191 KMKELGLSED-----------TVKNVIKGSDVSLPLGLYTFKDTEKSVVVDGNITTIKAL 239 Query: 270 DGVSVP-----------------------------------LSAIASVSRSLNDVQTMSR 294 + +P L IA + + + +++SR Sbjct: 240 KELKIPAIPSSPNGQGSQNAGAGAQTPQMNPAAMNGIPTVTLDEIADI-KEVGKAESISR 298 Query: 295 TDGKASVLINVIKTSGANITDVAKQVKTRVAALPAVEGGNVTMTLTGDRAHDLNASLLGL 354 T+GK ++ I ++K S AN DV VK +V L + ++ + T D+ + S+ + Sbjct: 299 TNGKEAIGIQIVKASDANTVDVVNAVKDKVKDLEK-KYKDLEIISTFDQGAPIEKSVETM 357 Query: 355 VREGLLGCVFSMLCVLFFFRNVRSTLLIAVSLPISLLATTAILKAMGVTLNILTVSGLIV 414 + + + G +F+++ ++ F RN+R+TL+ VS+P+SLL ++K M +TLNI+T+ + V Sbjct: 358 LSKAIFGAIFAIVIIMLFLRNIRTTLISVVSIPLSLLIAVLVIKQMDITLNIMTLGAMTV 417 Query: 415 AMGRIVDDAIVILDNMHRKMQ--EKKDQGALHVLAESVVEMLPAIFGSTATTVAVYAPIA 472 A+GR+VDD+IV+++N++R+M E+K +G ++ E+ EM I ST T+AV+ P+ Sbjct: 418 AIGRVVDDSIVVIENIYRRMSLSEEKLRGK-DLIREATKEMFIPIMSSTIVTIAVFLPLG 476 Query: 473 LVGGIIGASYSGFAWSVVIALAVSFLVAMLVIPAFAYMGWHKPGGKAVTLEPAMKP---- 528 LV G+IG + FA ++V AL S LVA+ ++P A+ + K + + KP Sbjct: 477 LVKGMIGEMFLPFALTIVFALLASLLVAVTIVPMLAHSLFKKESMREKEVHHEEKPSKLA 536 Query: 529 -----FLTTALKHKKTVLGVSLVIFIAAAFFASQLPFSLLPSTASGQVAVQLELPKGTAL 583 L AL HK +++++ + + + S LPS + G L Sbjct: 537 NRYKRILAWALNHKIITSSIAVLLLVGSLALVPVIGVSFLPSEEEKMIIATYNPEPGQTL 596 Query: 584 SEVDKAVQNVETVLKQDSQVAXXXXXXXXXXXPQADDVFDQGGGYIQQPN-IANLSVALK 642 +V+K E QD++ P + +Q ++Q N N K Sbjct: 597 EDVEKIATKAEKHF-QDNKDVKTIQFSLGGENPMSPGQSNQAMFFVQYDNDTKNFE---K 652 Query: 643 DQKQVNSYINSLQERLGNQQGVAAVTITNQNI--AGDDSTINIMLSGSDAGTLDKAASTV 700 +++QV + LQ+ G + NQ+ +G + I + + G + + + Sbjct: 653 EKEQV---VKDLQKMSGKGEW------KNQDFGASGGSNEIKLYVYGDSSEDIKPVVKDI 703 Query: 701 RSGLAGIEGLSVVGKTDLTNGIPKFSIALDRGKVLKAGVSRNDI---------NKVLSRY 751 ++ + + L + + + ++++ D+ K+ K G++ I VL+ Sbjct: 704 QNIMKKNKDLKDID-SSIAKTYAEYTLVADQEKLSKMGLTAAQIGMGLSNQHDRPVLTTI 762 Query: 752 MTKGKDFDISVSSGTGIIPVDVYLDPVKMGQAQAGGPLPVYTPDQVLASLAAETVKGKDG 811 GKD ++VY++ K + + L + G Sbjct: 763 KKDGKD-------------INVYVEAEKQNY-------------ETIDDLTNRKITTPLG 796 Query: 812 TVYRLDQLGTLQRSDAGSSIQERDGQPYTVVQARIVSSDTSGVSKAVNKVLRTTNLPEGV 871 + + T++ + +++ RDG+ Y V A++ S D S S AV K + +LP GV Sbjct: 797 NEVAVKDVMTVKEGETSNTVTHRDGRVYAEVSAKLTSDDVSKASAAVQKEVDKMDLPSGV 856 Query: 872 TYSLGGITQQVTQMIFEMIIAVVLSIFLVLLITSFVFRSWRAPIAVLISIPLALSGVVLA 931 S+GG+T+ + + ++ +A++ +I +V + F AP A+L S+P + G ++A Sbjct: 857 DVSMGGVTKDIEESFKQLGLAMLAAIAIVYFVLVVTFGGALAPFAILFSLPFTIIGALVA 916 Query: 932 LYTVHGQWNLAALIGVLMLTGIVVTNGIVLIDKIERNRREGLGLRDAVMQGSLSRVRPIF 991 L +++A+IG LML GIVVTN IVLID++ EGL R+A+++ +R+RPI Sbjct: 917 LLISGETLSVSAMIGALMLIGIVVTNAIVLIDRVIHKENEGLSTREALLEAGATRLRPIL 976 Query: 992 MTAATTILTLVPLTLSGDDGTVISKVLGVVVIGGMITSTLNSFVVIPILYEAMN 1045 MTA TI L+PL L + +ISK LGV VIGG+ +STL + +++PI+YE ++ Sbjct: 977 MTAIATIGALIPLALGFEGSGLISKGLGVTVIGGLTSSTLLTLLIVPIVYEVLS 1030 >ref|NP_347010.1| Cation efflux system protein, AcrB/AcrF/AcrD family [Clostridium acetobutylicum] pir||C96945 cation efflux system protein, AcrB/AcrF/AcrD family [imported] - Clostridium acetobutylicum gb|AAK78350.1| Cation efflux system protein, AcrB/AcrF/AcrD family [Clostridium acetobutylicum] Length = 1166 Score = 343 bits (881), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 236/786 (30%), Positives = 427/786 (54%), Gaps = 46/786 (5%) Query: 274 VPLSAIASVSRSLNDVQTMSRTDGKASVLINVIKTSGANITDVAKQVKTRVAALPAVEGG 333 V L +A+V+R D R++ K S+++N+ KT N +VAK V + + L G Sbjct: 413 VYLKDVATVTRGSADKSYFIRSNSKNSIILNIYKTDDGNTVNVAKDVASALEDLQKSNNG 472 Query: 334 NVTMTLTGDRAHDLNASLLGLVREGLLGCVFSMLCVLFFFRNVRSTLLIAVSLPISLLAT 393 + D + + S+ G+VREG+LG +F+++ + FF +N+++T++ VS+P+S+L Sbjct: 473 -IKFNKISDSSKYVKDSVNGMVREGVLGALFAIVVIAFFLKNIKATIIAVVSIPLSILIA 531 Query: 394 TAILKAMGVTLNILTVSGLIVAMGRIVDDAIVILDNMHRK-MQEKKDQGALHVLAESVVE 452 +L G+TLNI+++SG+ VA+GRIVDD+IV+++N+HR+ +++ ++G + +A E Sbjct: 532 LILLPRFGITLNIMSLSGMAVAVGRIVDDSIVVIENIHRRILKDDVEKGKIIQVAAD--E 589 Query: 453 MLPAIFGSTATTVAVYAPIALVGGIIGASYSGFAWSVVIALAVSFLVAMLVIPAFAYMGW 512 + AI ST TTVAV+ P+A++ G++G + FA +VVI + S LVA+ V+P + + Sbjct: 590 VSSAITSSTVTTVAVFLPLAMISGMVGKVFVPFAVTVVICILASLLVAVTVVPVMSRLMI 649 Query: 513 HKPGGKAVTLEPAM----KPFLTTALKHKKTVLGVSLVIFIAAAFFASQLPFSLLPSTAS 568 K E A+ K L AL H+ V+GVS+V+F+ + F + LPS++S Sbjct: 650 LNEKPKPEKGEGAIVGFYKKVLNGALNHRAAVMGVSVVLFVLSLFLIKGIGIQFLPSSSS 709 Query: 569 GQVAVQLELPKGTALSEVDKAVQNVETVLKQDSQVAXXXXXXXXXXXPQADDVFDQGGGY 628 + + GT+ + ++ E L S V + + QGG Sbjct: 710 NVLNGTITTAPGTSAEKTNEEALKFEKYLLNRSDVKTVVSSIGDNSSSSSSAMSMQGG-- 767 Query: 629 IQQPNIANLSVALKD----QKQVNSYINSLQERLGNQQGVAAVTITNQNIAGDDSTINIM 684 N +++ LKD K + I + + ++ AVT+ + +G + I+ Sbjct: 768 ----NSGKITIVLKDGVNNDKSADEIIKKATD-MSSKNTKFAVTVQSFT-SGSKDNVEIV 821 Query: 685 LSGSDAGTLDKAASTVRSGLAGIEGLSVVGKTDLTNGIPKFSIALDRGKVLKAGVSRNDI 744 ++G++ + AAS L G++ L+ V T L++ P+ S+A+D K G+S Sbjct: 822 VNGNNMKDITAAASKATEALKGVKELTNVNNT-LSSKKPEISVAIDSAKAANEGLS---- 876 Query: 745 NKVLSRYMTKG-KDFDISVSSGTGIIPVDVYLDPVKMGQAQAGGPLPVYTPDQVLASLAA 803 +++ M +G +++ + +G V+VYL Y + + Sbjct: 877 -PMIAAGMVQGIMNYNNVTTIQSGKNNVNVYLG---------------YDNKDINSLDKV 920 Query: 804 ETVK--GKDGTVYRLDQLGTLQRSDAGSSIQERDGQPYTVVQARIVSSDTSGVSK-AVNK 860 +T++ G G+ + L + +Q +D S++E +G Y + A + + DT SK A+ K Sbjct: 921 KTIQLQGASGS-FNLSDIADVQVADGPVSVEELNGNQYASITADVKTKDTQTASKNAMEK 979 Query: 861 VLRTTNLPEGVTYSLGGITQQVTQMIFEMIIAVVLSIFLVLLITSFVFRSWRAPIAVLIS 920 + +P GVT++ G + ++ +M +A+++++F+V ++ F +AP A+L S Sbjct: 980 IKSIKGIPNGVTFTQNGSAKSISDSFSQMGMAMLVAVFMVYIVMVLAFGEPKAPFAILFS 1039 Query: 921 IPLALSGVVLALYTVHGQWNLAALIGVLMLTGIVVTNGIVLIDKIERNRREGLGLRDAVM 980 +P A G ++AL+ + LIG+LML GIVVTN IVL+D+++ NR++G+ +R+A++ Sbjct: 1040 LPFAAIGAIVALFITRQPLGIPGLIGMLMLIGIVVTNAIVLLDRVQSNRKKGMLVREALI 1099 Query: 981 QGSLSRVRPIFMTAATTILTLVPLTLSGDDGTVISKVLGVVVIGGMITSTLNSFVVIPIL 1040 + R+RPIFMTA T++ L PL +G VIS+ LG+VVIGG++ ST+ + +++P++ Sbjct: 1100 EAGAIRMRPIFMTAIATVMALTPLAAGFSEGAVISQGLGIVVIGGLVVSTILTLIIVPVM 1159 Query: 1041 YEAMNR 1046 Y + R Sbjct: 1160 YSVLER 1165 Score = 70.1 bits (170), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 47/178 (26%), Positives = 85/178 (47%), Gaps = 3/178 (1%) Query: 47 GAVSAFQMQRDYLPGINNTTLMVSLRASAYQADQVKRDITTPIEQAIRKTNGILNVETTS 106 G S + + +P IN + +QV DI+ P+++AI GI +V+TTS Sbjct: 26 GLYSTKTINMESMPNINIPVVTAVTVYPGASPEQVASDISRPVQKAISGIQGIQDVKTTS 85 Query: 107 YDGGLLMNLYYPMNYNMDKAEQDIRMALGDADLPANVNAPTVTRLTTSTFPVLSYSLTAN 166 + ++ + + +MDKA++++ A+ P + T++R++ + P+++YS+ Sbjct: 86 NENVSVVVAQFDYSADMDKAQKNVEDAVNKIKFPESAKKSTISRISMGSAPIMTYSI--- 142 Query: 167 NEKVDDDTLRSTVQAGIVNQLKSVPGVSDVQAVGGAKNGYVVLIHTKDLSAAGLTLDD 224 N D L V I +L + GVS V+ G + + V + L GLTL D Sbjct: 143 NSTKSIDELTKIVNDKIEPKLSGISGVSSVEMQGTSNDDIYVKVDNDKLKNNGLTLSD 200 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,392,542,189 Number of extensions: 56006193 Number of successful extensions: 234559 Number of sequences better than 1.0e-05: 2259 Number of HSP's gapped: 229003 Number of HSP's successfully gapped: 3439 Length of query: 1061 Length of database: 757,237,578 Length adjustment: 136 Effective length of query: 925 Effective length of database: 387,984,114 Effective search space: 358885305450 Effective search space used: 358885305450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)