BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0512.0012.p (360 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00073034.1| hypothetical protein [Trichodesmium erythraeu... 67 5e-10 ref|ZP_00061324.1| COG3401: Fibronectin type 3 domain-containing... 62 3e-08 ref|NP_831113.1| Internalin [Bacillus cereus ATCC 14579] >gi|298... 59 2e-07 ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella th... 59 2e-07 gb|AAO88279.1| endo-beta-N-acetylglucosaminidase [Bacillus circu... 58 3e-07 >ref|ZP_00073034.1| hypothetical protein [Trichodesmium erythraeum IMS101] Length = 422 Score = 67.4 bits (163), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 57/186 (30%), Positives = 87/186 (46%), Gaps = 16/186 (8%) Query: 183 FSDVPVNHPYAKSIYYLVQLGAIPGGTN-EFKPDKAVTRGQFLSQIMPLAGFQLSTRPSV 241 FSDVP + + I LV+ I N +F+PDK VTR +F +QI + + + + V Sbjct: 226 FSDVPNSFWASSFIQSLVEQDFIAQINNDQFEPDKPVTRAEFAAQIAKVFEEKSAKKSVV 285 Query: 242 FSDTKG-SPYEAVIQTAVDYGIVQGLPGSIFGPNQPLTREQAATFIWRMVKISLNADP-- 298 + D KG S ++ IQT+ G + G PG +F P + ++R Q + + + + +DP Sbjct: 286 YKDIKGDSTAQSEIQTSTKSGFLSGYPGDVFRPEEKVSRLQVLVSLASGLSLEIPSDPDS 345 Query: 299 ---VKADLKTPASPWASEGVQYIAGQQLF--GPDVQAANGALEYRPKDPMLNKEAAVLIY 353 V D T WA E V +L PDV+ N P P E A + Y Sbjct: 346 VLSVYKDT-TEIPDWAKEKVAAATAAELVVSHPDVKMLN------PNQPATRAEVAAVFY 398 Query: 354 KLVQKL 359 + + KL Sbjct: 399 QALVKL 404 >ref|ZP_00061324.1| COG3401: Fibronectin type 3 domain-containing protein [Clostridium thermocellum ATCC 27405] Length = 1042 Score = 61.6 bits (148), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 59/202 (29%), Positives = 94/202 (46%), Gaps = 15/202 (7%) Query: 168 NMTDSWAEKIPVPPIFSDVPVNHPYAK-SIYYLVQLGAIPGGTNE---FKPDKAVTRGQF 223 N T+ K + F+D+ + P+AK +I L G I G + E F P+ +TR +F Sbjct: 846 NYTNIGMAKTALSKTFNDLN-SVPWAKEAIESLAARGIIHGKSEEQGIFAPNDRITRAEF 904 Query: 224 LSQIMPLAGFQLSTRPS-VFSDTK-GSPYEAVIQTAVDYGIVQGLPGSIFGPNQPLTREQ 281 ++ I + FQLS P+ F+D K Y + A + GIV G + F P+ P+ RE Sbjct: 905 ITLI--VNAFQLSKTPTGTFADVKPNHWYYRNVMIAKNMGIVSGTGNNYFYPDDPIKRED 962 Query: 282 AATFIWRMVKISLNADPVKADLKTPASPWASEGVQYIAGQQ----LFGPDVQAANGALEY 337 A + + KI P +D + ++ + + I Q L G + + + Sbjct: 963 MAVILAKTFKIIGKPLPNHSD--SVLDKYSDKNLISIYALQSMAILNGEGIITGKSSSQL 1020 Query: 338 RPKDPMLNKEAAVLIYKLVQKL 359 PKD EAAV++YK + KL Sbjct: 1021 SPKDYATRAEAAVILYKALNKL 1042 >ref|NP_831113.1| Internalin [Bacillus cereus ATCC 14579] gb|AAP08314.1| Internalin [Bacillus cereus ATCC 14579] Length = 760 Score = 59.3 bits (142), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 41/147 (27%), Positives = 66/147 (44%), Gaps = 2/147 (1%) Query: 183 FSDVPVNHPYAKSIYYLVQLGAIPG-GTNEFKPDKAVTRGQFLSQIMPLAGFQLS-TRPS 240 F DVP H ++I YL + G G EF +TRGQ I + + + + Sbjct: 588 FKDVPKGHWSEEAINYLAKEKLFIGYGNGEFGFGDNITRGQVALLIQRYLKLENNLEQKT 647 Query: 241 VFSDTKGSPYEAVIQTAVDYGIVQGLPGSIFGPNQPLTREQAATFIWRMVKISLNADPVK 300 F+DTKG+ YE I V GI+ G +F P+ LTR + + + R+ ++ N + + Sbjct: 648 AFTDTKGNMYETAIDAVVQAGIMTGYGNGMFRPDGVLTRYEMSVVLQRVFQLKENENSAE 707 Query: 301 ADLKTPASPWASEGVQYIAGQQLFGPD 327 P WA V+ + ++ D Sbjct: 708 NFKDVPNGHWAKGYVKALVDNKISKGD 734 >ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella thermoacetica ATCC 39073] Length = 368 Score = 58.9 bits (141), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 50/185 (27%), Positives = 84/185 (45%), Gaps = 22/185 (11%) Query: 183 FSDV-PVNHPYAK-SIYYLVQLGAIPG-GTNEFKPDKAVTRGQFLSQIMPLAGFQ-LSTR 238 F DV P + +A+ +I L G + G + F+P +AVTR +F S ++ G + + Sbjct: 192 FLDVTPASFGWAQDTIETLAGAGIVAGVDGSHFEPGRAVTRAEFASLLVKALGLEGKAGE 251 Query: 239 PSVFSDTKGSP-YEAVIQTAVDYGIVQGLPGSIFGPNQPLTREQAATFIWRMVKISLNAD 297 + F D KG Y + A G+V+G F P+ +TRE+ A + R V + + Sbjct: 252 KNPFKDVKGDAWYAGAVTAAASNGLVKGYEDGTFRPDNVITREELAAMLVRAVNLPAGEE 311 Query: 298 PVKADLKTPASPWASEGVQYIAGQQLFGPDVQAANGALE------YRPKDPMLNKEAAVL 351 + S WA + V V AA+G ++ +RP D E AV+ Sbjct: 312 KLAFKDNDKVSTWARKSVA-----------VAAAHGLVKGFEDGTFRPGDAASRAECAVM 360 Query: 352 IYKLV 356 +Y+++ Sbjct: 361 VYRVL 365 >gb|AAO88279.1| endo-beta-N-acetylglucosaminidase [Bacillus circulans] Length = 1936 Score = 58.2 bits (139), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 50/190 (26%), Positives = 83/190 (43%), Gaps = 15/190 (7%) Query: 177 IPVPPIFSDVPVNHPYAKSIYYLVQLGAIPGGTNE--FKPDKAVTRGQFLSQIMPLAGFQ 234 + V +F DVP H +A+ + + + GT+E F+P++ VTR +F + + Sbjct: 1754 LKVVKVFDDVPAGH-WAEGVISKLTSRLMVDGTSETTFEPERVVTRAEFTALLARALKLT 1812 Query: 235 LSTRPSVFSDTK-GSPYEAVIQTAVDYGIVQGLPGSIFGPNQPLTREQAATFIWRMVKIS 293 P+ F+D K G Y + AV+ GI +G F P +TRE+ R + Sbjct: 1813 AGGTPT-FADVKAGDWYADAVTAAVEAGIAEGKSAGQFEPQARITREEMVVMTMRAYNKA 1871 Query: 294 LNADP---VKADL--KTPASPWASEGVQYIAGQQLFGPDVQAANGALEYRPKDPMLNKEA 348 + P V+A + S WA E V+ A QL Q ++ P+ EA Sbjct: 1872 KDKGPSTGVEASFTDENQISAWAVEQVKAAAALQLIQGRAQG-----KFEPQGTATRAEA 1926 Query: 349 AVLIYKLVQK 358 +I+ ++ K Sbjct: 1927 VQVIFNMLLK 1936 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 596,569,919 Number of extensions: 26736222 Number of successful extensions: 45940 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 46005 Number of HSP's successfully gapped: 20 Length of query: 360 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 233 Effective length of database: 412,420,005 Effective search space: 96093861165 Effective search space used: 96093861165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)