BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0512.0008.p (319 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00097140.1| COG4548: Nitric oxide reductase activation pr... 387 e-106 ref|ZP_00038024.1| COG4548: Nitric oxide reductase activation pr... 261 2e-68 ref|NP_371932.1| conserved hypothetical protein [Staphylococcus ... 72 2e-11 ref|NP_646115.1| conserved hypothetical protein [Staphylococcus ... 72 2e-11 ref|NP_691763.1| hypothetical protein OB0842 [Oceanobacillus ihe... 72 2e-11 >ref|ZP_00097140.1| COG4548: Nitric oxide reductase activation protein [Desulfitobacterium hafniense] Length = 678 Score = 387 bits (995), Expect = e-106, Method: Compositional matrix adjust. Identities = 181/318 (56%), Positives = 242/318 (76%), Gaps = 1/318 (0%) Query: 2 ERSDTEEDGAPRVHQANPELKSEEARQQERDFRKKLNREARETVSGSIHSKIKLIVHRPD 61 + SD E+G P H+ P EE +Q+RDFRK LN++ARE V GS H K+ LIVHRP+ Sbjct: 362 QSSDQGENGNPIYHELQPT-SPEELERQKRDFRKHLNQQAREMVKGSCHEKVNLIVHRPE 420 Query: 62 FDLDSKREYQSLSRELLPVVREIARKTMPLLAHDDSFDFAKHQYYGSKFQADSIAYRDFR 121 L +REY+ ++ LLPV+RE+ RKT PLL H+ + +F K + YG+KF A+ +A DFR Sbjct: 421 ATLAHRREYEQIASGLLPVIRELVRKTAPLLEHEVATEFGKSRLYGTKFHAEKLASPDFR 480 Query: 122 YFAKKRPPTDSPSLAVGVRVDESASMSAFGRLAAAKQAVIAVYEFCQLCRIPVLIYGDTA 181 F++K PP + PSLAV +R+DESASMSAFGRL AAKQA +AVYEFC C IP LIYGDTA Sbjct: 481 CFSQKCPPNEEPSLAVALRIDESASMSAFGRLTAAKQAAVAVYEFCTSCGIPTLIYGDTA 540 Query: 182 DVSRLEQMSMYAYADFEHPDANDRFRLMGIRARSNNRDGMALRVMADRLAGSAAQTRLLI 241 D S LE+MS+Y+Y D+E+P ND++ LM I+ SNNRDGMALR+++++L + +T+LLI Sbjct: 541 DRSPLERMSLYSYIDYENPSLNDKYSLMAIQGHSNNRDGMALRILSEKLLQAPQKTKLLI 600 Query: 242 SISDGQPKALEDYTGSLAVQDMQQTLAYYERKGVTFLAAAIGQDKDVIHDIYGSDRFLDI 301 SISDGQPKAL DYTG +A+ DM++ + Y R+G+ FLAAAIGQDK+ + DIYG +R+LDI Sbjct: 601 SISDGQPKALPDYTGEVALADMKEVIRDYSRQGIVFLAAAIGQDKETLCDIYGPERYLDI 660 Query: 302 SHLPELPAQLVRMIARYL 319 + L +LPA+LV++IA+YL Sbjct: 661 TDLRQLPARLVQVIAKYL 678 >ref|ZP_00038024.1| COG4548: Nitric oxide reductase activation protein [Enterococcus faecium] Length = 742 Score = 261 bits (666), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 137/300 (45%), Positives = 202/300 (67%), Gaps = 1/300 (0%) Query: 20 ELKSEEARQQERDFRKKLNREARETVSGSIHSKIKLIVHRPDFDLDSKREYQSLSRELLP 79 E+ + + +++ +K+LN+EAR+ + +IH K LIVHRP F + E + L +++P Sbjct: 444 EMSASDMEAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTP-EARELHMQVMP 502 Query: 80 VVREIARKTMPLLAHDDSFDFAKHQYYGSKFQADSIAYRDFRYFAKKRPPTDSPSLAVGV 139 +V ++R+ + LL ++ + + K +Y G +F A +AY D R F KK PP + PSLAV V Sbjct: 503 IVESLSRQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAV 562 Query: 140 RVDESASMSAFGRLAAAKQAVIAVYEFCQLCRIPVLIYGDTADVSRLEQMSMYAYADFEH 199 R+DES SM R+ AAK+A IA+ EF + IP+LIYGDTAD S E+ S+++Y +FE Sbjct: 563 RIDESGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFED 622 Query: 200 PDANDRFRLMGIRARSNNRDGMALRVMADRLAGSAAQTRLLISISDGQPKALEDYTGSLA 259 +L+ ++ R NNRDG ALR+ A++L +A T+LL++ISDGQPKAL DYTG A Sbjct: 623 GFQWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA 682 Query: 260 VQDMQQTLAYYERKGVTFLAAAIGQDKDVIHDIYGSDRFLDISHLPELPAQLVRMIARYL 319 +D+Q L YER+G+ F++AAIGQDK+ I IYG RF+DI+ L E P Q++++IARYL Sbjct: 683 KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIARYL 742 >ref|NP_371932.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] ref|NP_374521.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus N315] pir||H89917 conserved hypothetical protein SA1240 [imported] - Staphylococcus aureus (strain N315) dbj|BAB42500.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus N315] dbj|BAB57570.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] Length = 628 Score = 72.0 bits (175), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 71/281 (25%), Positives = 129/281 (45%), Gaps = 34/281 (12%) Query: 45 VSGSIHSKIKLIVHRPDFDLDSKREYQSLSRELLPVVREIARKTMPLLAHDDSFDFAKHQ 104 V+ ++ K ++ P + L+ + Q + E+ ++ +I +KT+ D F+ K Sbjct: 347 VNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLI-QIIKKTIEREQRDARFNLTK-- 403 Query: 105 YYGSKFQADSIAY---RDFRYFAKKRPPTDSPSLAVGVRVDESASMSAFGRLAAAKQAVI 161 + Q D I + ++ F KK+ + S + +D SASM ++A K+ V+ Sbjct: 404 ---GRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMH--DKMAETKKGVV 458 Query: 162 AVYEFCQLCRIP--VLIYG-------DTADVSRLEQMSMYAYADFEHPDANDRFRLMGIR 212 +E + I +L + D A + + ++ Y Y+ FE D R+M + Sbjct: 459 LFHETLKALNIKHEILSFSEDAFDSDDHAQPNIINEIINYDYSTFE----KDGPRIMALE 514 Query: 213 ARSNNRDGMALRVMADRLAGSAAQTRLLISISDGQPKALEDYTGSLAVQDMQQTLAYYER 272 + +NRDG+A+RV ++RL R LI SDG+P A +Y+ + D + + + Sbjct: 515 PQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAF-NYSQD-GIIDTYEAVEMSRK 572 Query: 273 KGVTFLAAAIGQD---KDV---IHDIYGSDRFL--DISHLP 305 G+ + QD +DV IH+IYG ++HLP Sbjct: 573 FGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLP 613 >ref|NP_646115.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus MW2] dbj|BAB95163.1| conserved hypothetical protein [Staphylococcus aureus subsp. aureus MW2] Length = 628 Score = 72.0 bits (175), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 71/281 (25%), Positives = 129/281 (45%), Gaps = 34/281 (12%) Query: 45 VSGSIHSKIKLIVHRPDFDLDSKREYQSLSRELLPVVREIARKTMPLLAHDDSFDFAKHQ 104 V+ ++ K ++ P + L+ + Q + E+ ++ +I +KT+ D F+ K Sbjct: 347 VNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLI-QIIKKTIEREQRDARFNLTK-- 403 Query: 105 YYGSKFQADSIAY---RDFRYFAKKRPPTDSPSLAVGVRVDESASMSAFGRLAAAKQAVI 161 + Q D I + ++ F KK+ + S + +D SASM ++A K+ V+ Sbjct: 404 ---GRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMH--DKMAETKKGVV 458 Query: 162 AVYEFCQLCRIP--VLIYG-------DTADVSRLEQMSMYAYADFEHPDANDRFRLMGIR 212 +E + I +L + D A + + ++ Y Y+ FE D R+M + Sbjct: 459 LFHETLKALNIKHEILSFSEDAFDSDDHAQPNIINEIINYDYSTFE----KDGPRIMALE 514 Query: 213 ARSNNRDGMALRVMADRLAGSAAQTRLLISISDGQPKALEDYTGSLAVQDMQQTLAYYER 272 + +NRDG+A+RV ++RL R LI SDG+P A +Y+ + D + + + Sbjct: 515 PQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAF-NYSQD-GIIDTYEAVEMSRK 572 Query: 273 KGVTFLAAAIGQD---KDV---IHDIYGSDRFL--DISHLP 305 G+ + QD +DV IH+IYG ++HLP Sbjct: 573 FGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLP 613 >ref|NP_691763.1| hypothetical protein OB0842 [Oceanobacillus iheyensis HTE831] dbj|BAC12798.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 638 Score = 72.0 bits (175), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 81/319 (25%), Positives = 141/319 (44%), Gaps = 30/319 (9%) Query: 23 SEEARQQERDFRKKLNR--EARETVSGSIHSK-----IKLIVHRPDFDLDSKREYQSLSR 75 S +++QQ+ ++ L++ E + SGS + K + LI LD + +Y Sbjct: 324 SVQSKQQDYSDKETLSKQEEQQSGNSGSEYGKENEDAVMLIEKAEHPSLDDQEKYDQYVV 383 Query: 76 ELLPVVREIARKTMPLLAHDDSFDFAKHQYYG--SKFQADSIAYRDFRYFAKKRPPTDSP 133 E+ P R++A+ L H +F + +G SK I R F KK + Sbjct: 384 EVDPYKRKLAKTIQLTLEHKRNFPRTE-LLFGRLSKKLLPIIIDESPRVFYKKEQESKEF 442 Query: 134 SLAVGVRVDESASMSAFGRLAAAKQAVIAVYEFCQLCRIPVLIYGDTADVSRLEQMSMYA 193 + +D SASM ++ K+ V+ +E + RIP I G D + +++ Sbjct: 443 DAVFTLLIDCSASM--VNKMEETKRGVVLFHEVLKELRIPHEIIGFWEDANEVKENYQPN 500 Query: 194 YADFEHPDANDRF------RLMGIRARSNNRDGMALRVMADRLAGSAAQTRLLISISDGQ 247 Y + H + ND ++M + + +NRDG ++R + +L + + L+ SDG+ Sbjct: 501 YLQYIH-ELNDSLYENNGAKIMQLEPQEDNRDGFSIRYITKQLMKRNEKNKFLLVFSDGE 559 Query: 248 PKALE-DYTGSLAVQDMQQTLAYYERKGV----TFLAAAIGQDKD--VIHDIYGSDRFLD 300 P A D G L D Q + + + FLA Q+K+ + +IYG +R + Sbjct: 560 PAASGYDQNGIL---DTHQAVTEARKHHIDVIGMFLANGEIQEKNDTTMKNIYGKERIM- 615 Query: 301 ISHLPELPAQLVRMIARYL 319 I H+ ELP ++ R L Sbjct: 616 IPHVEELPEHFTPLLKRLL 634 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.134 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 443,229,482 Number of extensions: 17224520 Number of successful extensions: 46001 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 46007 Number of HSP's successfully gapped: 15 Length of query: 319 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 194 Effective length of database: 417,850,203 Effective search space: 81062939382 Effective search space used: 81062939382 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)