BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0510.0011.p (221 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242993.1| BH2127~unknown conserved protein [Bacillus halo... 144 2e-33 ref|NP_346846.1| Sortase (surface protein transpeptidase), YHCS ... 95 1e-18 ref|NP_781831.1| conserved protein [Clostridium tetani E88] >gi|... 77 3e-13 ref|NP_242881.1| BH2015~unknown conserved protein [Bacillus halo... 73 5e-12 ref|NP_241228.1| BH0362~unknown conserved protein [Bacillus halo... 72 9e-12 >ref|NP_242993.1| BH2127~unknown conserved protein [Bacillus halodurans] pir||G83915 hypothetical protein BH2127 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05846.1| BH2127~unknown conserved protein [Bacillus halodurans] Length = 194 Score = 144 bits (362), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 81/213 (38%), Positives = 118/213 (55%), Gaps = 31/213 (14%) Query: 10 LIILAGILLMLYPK-ANEWYNDWXXXXXXXXXXXXYSEISPSPSPQSDLHKQYAEVTQLL 68 L I G+L M +P+ E+Y+ + ++ L Y ++ + L Sbjct: 8 LFISFGLLFMFFPRLQTEYYSFY----------------------ETRLIDSYEKINREL 45 Query: 69 AEESSEGDLAKATEAPAPEIKLGGKITGIIQIDKINLKLPVLEGXXXXXXXXXXXXXXET 128 S+E ++E P G+++I+KI+LKLPVL+G E+ Sbjct: 46 EAFSNEEPHLLSSEEP--------HAIGVLEIEKISLKLPVLQGVDQETLKVGAGHMIES 97 Query: 129 AAIGQVGNAAIAAHRSRTAGRLFNRLNEVEVGDTISVQTKEQDYQYVVYDISIVEPTDVS 188 + IG+ GNAAIAAHRSRT GR FNRL+EVEVGD I+V T Y+Y VY I++VEPT++ Sbjct: 98 SPIGEKGNAAIAAHRSRTYGRQFNRLDEVEVGDVITVTTNNHMYRYTVYSITVVEPTNID 157 Query: 189 VLNGHNDDKILTLITCDPLVNPTHRLIIHARLS 221 +L +LTLITCDP+ +PTHRLI+ A ++ Sbjct: 158 ILQHDGTAPVLTLITCDPVKDPTHRLIVQAEMT 190 >ref|NP_346846.1| Sortase (surface protein transpeptidase), YHCS B.subtilis ortholog [Clostridium acetobutylicum] pir||G96924 sortase (surface protein transpeptidase), YHCS B. subtilis ortholog [imported] - Clostridium acetobutylicum gb|AAK78186.1| Sortase (surface protein transpeptidase), YHCS B.subtilis ortholog [Clostridium acetobutylicum] Length = 194 Score = 94.7 bits (234), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 51/124 (41%), Positives = 70/124 (56%), Gaps = 1/124 (0%) Query: 96 GIIQIDKINLKLPVLEGXXXXXXXXXXXXXXETAAIGQVGNAAIAAHRSRTAGRLFNRLN 155 GI++I KI+LK+ + EG T+ GQ GN +A HRS T G FNRL Sbjct: 70 GILKIPKIDLKVAIGEGTDLKTLKYAVGHFRNTSMPGQNGNFCLAGHRSYTFGEYFNRLG 129 Query: 156 EVEVGDTISVQTKEQDYQYVVYDISIVEPTDVSVLNGHNDDKILTLITCDPLVNPTHRLI 215 E+ GD I V+T ++Y VY +V P++V VL+ D +TL+TC P+ THRLI Sbjct: 130 EIGSGDEIDVETVNGTFKYKVYSTKVVLPSEVHVLD-QTKDPTMTLVTCTPIRIATHRLI 188 Query: 216 IHAR 219 I A+ Sbjct: 189 IKAK 192 >ref|NP_781831.1| conserved protein [Clostridium tetani E88] gb|AAO35768.1| conserved protein [Clostridium tetani E88] Length = 199 Score = 77.0 bits (188), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 42/126 (33%), Positives = 69/126 (54%), Gaps = 2/126 (1%) Query: 96 GIIQIDKINLKLPVLEGXXXXXXXXXXXXXXETAAIGQVGNAAIAAHRSRTAGRLFNRLN 155 II+I I LK ++EG ETA GQ+GN IA H S + N L+ Sbjct: 72 AIIEIPSIGLKSVIVEGTEMEKLRYYIGHFKETALPGQLGNFCIAGHSSSMYSEILNNLH 131 Query: 156 EVEVGDTISVQTKEQDYQYVVYDISIVEPTDVSVLNGHNDDKILTLITCDPLVNPTHRLI 215 +V++GD I ++T ++++ Y + + +V+PT VSVLN + +T++TC N +RLI Sbjct: 132 KVKIGDEIKIKTIKEEFNYNIKEKFVVDPTKVSVLNQDKTKRSMTIVTCTD--NGKNRLI 189 Query: 216 IHARLS 221 + ++ Sbjct: 190 VKGEIN 195 >ref|NP_242881.1| BH2015~unknown conserved protein [Bacillus halodurans] pir||G83901 hypothetical protein BH2015 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05734.1| BH2015~unknown conserved protein [Bacillus halodurans] Length = 193 Score = 72.8 bits (177), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 42/107 (39%), Positives = 57/107 (53%), Gaps = 1/107 (0%) Query: 98 IQIDKINLKLPVLEGXXXXXXXXXXXXXXETAAIGQVGNAAIAAHRSRTAGRLFNRLNEV 157 ++I I LK VL E G+ GN AIA HR R F+RL +V Sbjct: 66 LEIPSIELKQKVLSETTEENLKLALTQLKENQHPGE-GNFAIAGHRGYRGNRHFSRLPDV 124 Query: 158 EVGDTISVQTKEQDYQYVVYDISIVEPTDVSVLNGHNDDKILTLITC 204 +GD + + TKE+ + Y V DISI+EPTDV VL+ + +T+ITC Sbjct: 125 TIGDEVFLHTKEETFVYKVTDISIIEPTDVDVLDDRDGKHEITMITC 171 >ref|NP_241228.1| BH0362~unknown conserved protein [Bacillus halodurans] pir||B83695 hypothetical protein BH0362 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04081.1| BH0362~unknown conserved protein [Bacillus halodurans] Length = 187 Score = 72.0 bits (175), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 41/107 (38%), Positives = 57/107 (53%), Gaps = 1/107 (0%) Query: 98 IQIDKINLKLPVLEGXXXXXXXXXXXXXXETAAIGQVGNAAIAAHRSRTAGRLFNRLNEV 157 ++I I LK VL E G+ GN AIA HR R F+RL +V Sbjct: 66 LEIPSIELKQKVLSETTEENLKLALTQLKENQHPGE-GNFAIAGHRGYRGNRHFSRLPDV 124 Query: 158 EVGDTISVQTKEQDYQYVVYDISIVEPTDVSVLNGHNDDKILTLITC 204 +GD + + TKE+ + Y V DISI+EPTDV +L+ + +T+ITC Sbjct: 125 TIGDEVFLHTKEETFVYKVTDISIIEPTDVDILDDRDGKHEITMITC 171 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 269,807,566 Number of extensions: 9175792 Number of successful extensions: 22511 Number of sequences better than 1.0e-05: 78 Number of HSP's gapped: 22508 Number of HSP's successfully gapped: 78 Length of query: 221 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 100 Effective length of database: 428,710,599 Effective search space: 42871059900 Effective search space used: 42871059900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 123 (52.0 bits)