BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0510.0008.p (1262 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_346994.1| Uncharacterized membrane protein [Clostridium a... 200 2e-49 ref|ZP_00062207.1| COG1928: Dolichyl-phosphate-mannose--protein ... 139 4e-31 ref|ZP_00061268.1| hypothetical protein [Clostridium thermocellu... 126 4e-27 ref|NP_487190.1| hypothetical protein [Nostoc sp. PCC 7120] >gi|... 118 8e-25 ref|NP_769052.1| blr2412 [Bradyrhizobium japonicum] >gi|27350667... 118 1e-24 >ref|NP_346994.1| Uncharacterized membrane protein [Clostridium acetobutylicum] pir||C96943 uncharacterized membrane protein [imported] - Clostridium acetobutylicum gb|AAK78334.1| Uncharacterized membrane protein [Clostridium acetobutylicum] Length = 544 Score = 200 bits (509), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 123/387 (31%), Positives = 192/387 (49%), Gaps = 8/387 (2%) Query: 252 ILRIWIGLTAQGYQNDMNTFIAWGQRLVDLGPGKFYEEGYFADYPPGYLYILYVLSLIRG 311 + RI +GL GY D+ + W Y G DYPP YLY VLS+I Sbjct: 156 LFRIIVGLLITGYVTDIGLYKQWALTAAK-NLAAVYTNGS-VDYPPVYLY---VLSIIGK 210 Query: 312 LFGFAHGSGGETLLFKLPAILSDLVLGWLIYRAGRTKLGSGMAVGLMLLFLFNPAVLMDS 371 + + S TLL KLP+I++D+ + +YR + + L L +LFNPA+L++S Sbjct: 211 IASSSFMSNYSTLLIKLPSIIADIFSAYFVYRLSIKRFSYETSFALTLFYLFNPAILINS 270 Query: 372 AAWGQADSXXXXXXXXSILGASEQRFVRSAIFFALATLVKPQALIFTPVLLFAFYHHRAW 431 WGQADS ++ SE + V S+IFFAL+ L+KPQ +IF P++LF + ++ Sbjct: 271 TLWGQADSFFTLLVILAVYFISEDKIVPSSIFFALSVLMKPQGIIFLPLVLFWYIKNKNL 330 Query: 432 KQLAVGALYGLGIFAVLAAPFFWNNGGIGGLIDLYKGTLSSYPYSTVNAFNLYALTDPLW 491 K + G ++ APF + G I + DLY +S YPY++ NAFN Y+L Sbjct: 331 KSIFKSIASGFITTIIVLAPFSISKGNITWIFDLYTREVSEYPYASNNAFNFYSLIGANR 390 Query: 492 ASIDSMWLGITYRAWGFIFILAAVALASYYSFKKDRKELSKSFFIGMVLITIVFVFGTKM 551 + +Y WG +FI+ ++ ++ K K S ++ + VF F M Sbjct: 391 VKDSNTMFIFSYHTWGMLFIVLITLISGFFYLKSKNKYAST--LAALIQVVGVFNFSVGM 448 Query: 552 HERYMYPALLLCLFTYIESRDRRXXXXXXXXXXXQYLNVGYTL-AHLNAGNNPPSDGIVL 610 HERYM+ A+L + YI +D+R Y N+ L LN N S +++ Sbjct: 449 HERYMFTAVLASILAYIYFKDKRLLLISLLLSFINYANMHTILFNRLNMNVNSSSSMLIV 508 Query: 611 VTAIANIALALYMLYVGYAVYVRKDIK 637 ++ NI +Y++ V + + ++KDIK Sbjct: 509 FISLLNITTFVYLIKVCFDLCLKKDIK 535 >ref|ZP_00062207.1| COG1928: Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Clostridium thermocellum ATCC 27405] Length = 967 Score = 139 bits (351), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 100/304 (32%), Positives = 148/304 (48%), Gaps = 26/304 (8%) Query: 588 LNVGYTLAHLNAGNNPPSDGIVLVTAIANIALALYMLYVGYAVYVRKDIKPILPPAAAAE 647 L+ YT + N N + G + + IA I L +L V + ++ + + AA + Sbjct: 203 LDPKYTGGNQNTSEN--TGGKIFMIFIALIFF-LILLAVIFVIFFKDKMPGFNAKAAQTD 259 Query: 648 KYDADLELAEGMRPLPKSASRIGLPKLQRKDWIWMLGITAVYAALALFHLGSTKAPETLW 707 K A + K K + D+I M +T VY +AL++LG K P T W Sbjct: 260 KPAAKKNQKDNDEKWIKV-------KFDKIDFIIMGVMTLVYLCVALYNLGDFKVPTTSW 312 Query: 708 EPAASGESFVVDLGQNRQLERVNIFGGVGTGKF------KLEFSQSPDTWSGPLDVNE-D 760 EP GE F VDLG+ L R+ + G+G+ K+ ++E+ + +++ + Sbjct: 313 EPTLPGEYFTVDLGKEATLSRIYYYCGLGSNKYNPFSKLRVEYLNENQEFEYLATIDKKE 372 Query: 761 VGNVFIWK-SQPLNVAARYVKLTVTSPGFTLNEMAFYEQGGGKTPLP----VASVTPDST 815 + ++F WK V +K V S G NE+A EQ K PL + SV D + Sbjct: 373 LQDIFKWKYVDTAPVTTTQLKFIVDSTGGAFNEIAIVEQNS-KEPLKNIKIIDSVLEDES 431 Query: 816 TAKRGQPSNLFDEQSVIPENSGFMNSTYFDEIYHARTAYEYAHGIVPYENTHPPLGKLLI 875 A G NLFDE +MN YFDEIYHARTA+E+ H + PYE THPPLGK+ I Sbjct: 432 KATIG---NLFDEPDKFAFKPSYMNGMYFDEIYHARTAFEHIHRMSPYETTHPPLGKIFI 488 Query: 876 SVGM 879 ++G+ Sbjct: 489 ALGI 492 Score = 122 bits (305), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 64/177 (36%), Positives = 104/177 (58%), Gaps = 10/177 (5%) Query: 1045 NTIVTLLSCVVFFVIIPGVIYSLSFIPVLSVSADGYTFKGLIDAQKNMYNYHSQLVATHP 1104 N +T+ +CV+FF+IIP VIY LS+IP + V D I+ K+M+ YHS+L A HP Sbjct: 638 NLGITIGACVLFFIIIPLVIYILSYIPYMLVPGDNKGITVFIENSKHMFEYHSKLEAEHP 697 Query: 1105 FASSWWEWPFMKRPVWFFSGGEGLPEGQVSSIVTMGNPLIWWTGIFAMLASVWFTIKRKD 1164 + S+WWEWP M +P+ F+ G + L G S I TMGNP +WW G+ A+L F + + + Sbjct: 698 YQSAWWEWPIMAKPMAFYFGSD-LEPGMTSKIFTMGNPAVWWVGLLALLIVAIFALSKVN 756 Query: 1165 KNLYMIWIGFFSQYVPWMLVPRETFLYH--------YFAMVPFMILAIVYIMKLLDS 1213 KNL +++ + ++ +P+ F +A++ F ++AI+ ++ LD+ Sbjct: 757 KNLVVLFT-LAATTFGYVALPKTIFTETLKAQSAELCWAVIFFALIAILLVLSKLDT 812 Score = 93.2 bits (230), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 49/166 (29%), Positives = 80/166 (48%) Query: 17 NLLQNXXXXXXXXXXXXXWTKDMWIQGDSSGLLSVQSEEVHSGTRAAVIENLEPNHLKWV 76 NL++N W W + + + HSG ++A I N N +++ Sbjct: 39 NLVKNPGFEEGNDESVYFWQTHCWEKAEGVTEFFIDESVYHSGGKSACIVNHSENDSRYM 98 Query: 77 QTIAVSPNSYYKISGYVKIVSTAGQGLGANIFPVGIGGGYPATTDTGGDWQYLEFYGQTG 136 Q I V ++YY++S +VK + + GANI G +T +W+YLE YG+T Sbjct: 99 QPIKVKGDTYYRLSCWVKTENVGTKTKGANISIEGSLDTSRDIRETSDNWEYLELYGKTS 158 Query: 137 SGQKELGIGAALGGYASLIQGKAYFDDLSVEQVDAAPAGAGVISLD 182 Q+ + LGGY + GK + DD+ V ++++ PAG I+LD Sbjct: 159 PNQETFTLTIGLGGYGNTNTGKIWIDDVEVVELESLPAGKTAINLD 204 Score = 86.7 bits (213), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 45/120 (37%), Positives = 66/120 (55%), Gaps = 1/120 (0%) Query: 1143 LIWWTGIFAMLASVWFTIKRKDKNLYMIWIGFFSQYVPWMLVPRETFLYHYFAMVPFMIL 1202 LI W + + ++ I R DK + + G QYVPW+ VPR F+YHYF++VPF+IL Sbjct: 849 LIIWACLLICITLLFIGIYRYDKKMMVPLAGLIFQYVPWIAVPRIAFIYHYFSIVPFLIL 908 Query: 1203 AIVYIMKLLDSKYPEXXXXXXXXXXXXXXXFVMFYPVLSGMQVSKDYVDVVLRWFPSWVF 1262 IVY++K K+ F++FYP LSG++V Y+ L+WF +W F Sbjct: 909 LIVYVIKKAADKHSGVKYFACVYLGIVMALFILFYPGLSGLEVPTSYMR-YLKWFNTWYF 967 >ref|ZP_00061268.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 402 Score = 126 bits (316), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 95/331 (28%), Positives = 156/331 (47%), Gaps = 38/331 (11%) Query: 264 YQNDMNTFIAWGQRLVDLGPGKFYEEGYFADYPPGYLYILYVLSLIRGLFGFAHGSGGET 323 YQ DM + AW L + GP FYE Y Y P Y+Y+L++ +I F+ + Sbjct: 1 YQVDMGGYRAWSLYLAENGPVGFYER-YHVVYAPAYMYLLWITGIIAK--AFSVNASTHA 57 Query: 324 LLFKLPAILSDLVLGWLIYRAGRTKLGSGMAVGLMLLFLFNPAVLMDSAAWGQADSXXXX 383 L KL A+ S+LV +LIY+ G+ + L +++ NP V +S+ WGQ DS Sbjct: 58 FLIKLWAVASELVGAYLIYKIGKKYKKERLGFILGVVYALNPGVFFNSSIWGQFDSIPAT 117 Query: 384 XXXXSILGASEQRFVRSAIFFALATLVKPQALIFTPVLLFAFYHH-------------RA 430 I S R + + + +A+A L KPQ+ + TP+ + FY ++ Sbjct: 118 LLVGMIYAFSVNRKMTAVVLYAIAVLTKPQSALLTPLGIL-FYKELFDFSNITKEKIVKS 176 Query: 431 WKQLAVGALYGLGIFAVLAAPFFWNN-------------GGIGGLID-------LYKGTL 470 K+ V GL + ++ PF+++ I ID LY ++ Sbjct: 177 IKETLVAICVGLSCYFIVIYPFYYHTDLYERMKSTSVVKDFIAESIDYFWWMPNLYLTSV 236 Query: 471 SSYPYSTVNAFNLYALTDPLWASIDSMWLGITYRAWGFIFILAAVALASYYSFKKDRKEL 530 YPY+T NAFNL+ L +++ ++Y+ WG I L + +A Y KK + + Sbjct: 237 EDYPYATANAFNLWTLLGGQPVKDSNIFFILSYKTWGTILFLICIGIAFAYLLKKRKSDF 296 Query: 531 SKSFFIGMVLITIVFVFGTKMHERYMYPALL 561 + +F +++ F F T+MHERY+ PA++ Sbjct: 297 AM-YFASFFILSSAFTFITRMHERYLLPAII 326 >ref|NP_487190.1| hypothetical protein [Nostoc sp. PCC 7120] pir||AG2199 hypothetical protein alr3150 [imported] - Nostoc sp. (strain PCC 7120) dbj|BAB74849.1| ORF_ID:alr3150~hypothetical protein [Nostoc sp. PCC 7120] Length = 486 Score = 118 bits (296), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 117/479 (24%), Positives = 205/479 (42%), Gaps = 78/479 (16%) Query: 840 NSTYFDEIYHARTAYEYAHGIVPYENTHPPLGKLLISVGMELF--------GVN------ 885 N+ FDEIY+A+ + Y + I P+ + HPPLGK +I++GM L GVN Sbjct: 29 NTLVFDEIYYAKFGHNYLNYI-PFFDAHPPLGKYMIAMGMWLSSYIPFWQEGVNGLTGAL 87 Query: 886 --PFGWRIIGTLFGIAMLPLIYI-MALRLFKKSXXXXXXXXXXXXDFMHFTQTRISTIDV 942 P+ +R + L G + +PLI +A + + D + ++R + I++ Sbjct: 88 RSPWSYRWVNALSG-SFIPLIIANIAYHISYRRSFALIAGLFTALDGLFIVESRYALINI 146 Query: 943 YGVFFIMLMFYFMQRYFTMSFYRIPLRRTLVPLFWSGLFFGIGVASKWIVIYXXXXXXXX 1002 Y V F +L Q F ++ RR L L +G+ FG +A+KW ++ Sbjct: 147 YIVIFGLL----GQWLFLLALASQKQRRNLY-LVIAGMAFGCSIATKWNGLFYLVGIYLI 201 Query: 1003 XXXXXFERYREYRAAGRLLAEGRVGDQELRAACSTADKSFWKNTIVTLLSCVVFFVIIPG 1062 ++ + +E + D + S + T + + + F IIP Sbjct: 202 WSIAWVWQF--------ITSEYQPRDNSADVEPISLQTSLYNITQINIGQIIFFLGIIPT 253 Query: 1063 VIYSLSFIPVLSVSADGYTFKGLIDAQKNMYNYHSQL----VATHPFASSWWEWPFMKRP 1118 VIYSL +IP L ++ G I + + +H +L HP+ ++W++WP M RP Sbjct: 254 VIYSLIWIPHLQINPK----YGFIQVHQEILGFHERLDGNTAQVHPYCAAWYKWPLMIRP 309 Query: 1119 V-WFFSGGEGLPEGQ--------------VSSIVTMGNPLIWWTGIFAML-------ASV 1156 + +++ + + + + + MGNP +WW G+ A+L A V Sbjct: 310 MAYYYQTAQSVNDPLPVLGPPLPSGAGKVIYDVHAMGNPFLWWFGVAALLFLLGMLIAKV 369 Query: 1157 WFTIKRKDK-------------NLYMIWIGFFSQYVPWMLVPRETFLYHYFAMVPFMILA 1203 + ++ + +LY++W + +PW +V R F+YHY V F LA Sbjct: 370 MIPLVKERRLSLPAKLSVDTWISLYIVW-NYAINLLPWTIVSRCVFIYHYMTGVVFAFLA 428 Query: 1204 IVYIM-KLLDSKYPEXXXXXXXXXXXXXXXFVMFYPVLSGMQVSKDYVDVVLRWFPSWV 1261 I + + + L S Y + + PV G+ +S + + + WF SWV Sbjct: 429 IAWFVDQCLRSYYRPLRLVGVTISFVIIAALIFWLPVYLGLPLSPEGYKMRM-WFKSWV 486 >ref|NP_769052.1| blr2412 [Bradyrhizobium japonicum] dbj|BAC47677.1| blr2412 [Bradyrhizobium japonicum USDA 110] Length = 476 Score = 118 bits (295), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 97/371 (26%), Positives = 160/371 (43%), Gaps = 34/371 (9%) Query: 844 FDEIYHARTAYEYAHGIV--PYEN-THPPLGKLLISVGMELFGVNPFGWRIIGTLFGIAM 900 FDE+++ A + ++ P N HPPL K LI+ + FG N GWR TLFG Sbjct: 87 FDEVHYVPAARQMLAPVMSQPMLNPMHPPLAKELIAASIATFGDNALGWRYPATLFGALA 146 Query: 901 LPLIYIMALRLFKKSXXXXXXXXXXXXDFMHFTQTRISTIDVYGVFFIMLMFYFMQRYFT 960 + IY+ L LF + M + Q RI+ +D++ + F +L F Sbjct: 147 IVAIYLCGLALFAAQGPAIAAALIAAFNQMLYVQARIAMLDIFALGFGLLA----TAAFM 202 Query: 961 MSFYRIPLRRTLVPLFWSGLFFGIGVASKWIVIYXXXXXXXXXXXXXFERYREYRAAGRL 1020 F R R +G FG A KW ++ R + L Sbjct: 203 HGFRR---ERPHALFALAGGLFGCAAACKWSGLFPLGICIVIVAVI-----RLMQGWRTL 254 Query: 1021 LAEGRVGDQELRAACSTADKSFWKNTIVTLLSCVVFFVIIPGVIYSLSFIPVLSVSADGY 1080 A+ R D W + ++ + F ++P + Y +FIP+ G Sbjct: 255 FADARPDDWY--------RPDLWPDFRAPHVA--LCFAVLPAMTYLAAFIPLY-----GL 299 Query: 1081 TFKGLIDAQKNMY-NYHSQLVATHPFASSWWEWPFMKRPVWFFSGGEGLPEGQVSSIVTM 1139 + LI+ Q+ ++ + + +A H + SSW WP + RPVWF E VS+IV + Sbjct: 300 SLPDLIEGQRRIFADNTTTAIAGHTYMSSWPSWPLLARPVWFLFDKTA--EDSVSAIVFL 357 Query: 1140 GNPLIWWTGIFAMLASVWFTIKRKDKNLYMIWIGFFSQYVPWMLVPRET-FLYHYFAMVP 1198 GNPL+ W + A+ + + + + ++I +F ++ W L+PR F+Y+Y Sbjct: 358 GNPLVLWPALAALAIVLRDVVVARRWDAFLIAAFYFGPWLAWALLPRTLGFIYYYLPAAT 417 Query: 1199 FMILAIVYIMK 1209 LA+VY+++ Sbjct: 418 AASLALVYVLR 428 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.141 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,806,599,251 Number of extensions: 76287714 Number of successful extensions: 218627 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 218582 Number of HSP's successfully gapped: 54 Length of query: 1262 Length of database: 757,237,578 Length adjustment: 137 Effective length of query: 1125 Effective length of database: 385,269,015 Effective search space: 433427641875 Effective search space used: 433427641875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)