BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0051.p (425 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_346571.1| alcohol dehydrogenase, iron-containing [Strepto... 455 e-127 ref|NP_359554.1| Probable alcohol dehydrogenase. [Streptococcus ... 455 e-126 ref|NP_561365.1| alcohol dehydrogenase [Clostridium perfringens]... 368 e-100 ref|NP_936583.1| alcohol dehydrogenase, class IV [Vibrio vulnifi... 363 4e-99 ref|NP_762008.1| Alcohol dehydrogenase, class IV [Vibrio vulnifi... 363 4e-99 >ref|NP_346571.1| alcohol dehydrogenase, iron-containing [Streptococcus pneumoniae TIGR4] pir||B95252 alcohol dehydrogenase, iron-containing [imported] - Streptococcus pneumoniae (strain TIGR4) gb|AAK76211.1| alcohol dehydrogenase, iron-containing [Streptococcus pneumoniae TIGR4] Length = 383 Score = 455 bits (1171), Expect = e-127, Method: Compositional matrix adjust. Identities = 222/348 (63%), Positives = 267/348 (76%), Gaps = 2/348 (0%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK++ S I K L LD GI+Y ++ +V+PNPTCKNV DG+A L GCD++IS+GG Sbjct: 36 TDKYIEGSDILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGG 95 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSPQD A I I+ATNGG +DYEG+HKS K LP+VA+NTTAGTSAE+TINYVITDEER Sbjct: 96 GSPQDAASCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEER 155 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 KVKMVMVDKNS+A ISVNDPELM+ KP LTAATGMDALTHA+EALVTPGAY VT ++ Sbjct: 156 KVKMVMVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSI 215 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI L R V+NGHDIEARE MV AI YVH+MAHQLG VY+LPH Sbjct: 216 GAIELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPH 275 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVC AMLLP +E ENAK VPE FR++AKA+GL +EGKSD ECAD+ I I+ LS+ VGIP Sbjct: 276 GVCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGIP 335 Query: 380 SKLSELGVTEVDLDL--LAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 KL+ELG+ E D D L++N++ DACAPGNPF+PT EE I ++++ Sbjct: 336 KKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF 383 >ref|NP_359554.1| Probable alcohol dehydrogenase. [Streptococcus pneumoniae R6] pir||H98116 probable alcohol dehydrogenase (EC 1.1.1.1) [imported] - Streptococcus pneumoniae (strain R6) gb|AAL00765.1| Probable alcohol dehydrogenase. [Streptococcus pneumoniae R6] Length = 383 Score = 455 bits (1170), Expect = e-126, Method: Compositional matrix adjust. Identities = 222/348 (63%), Positives = 266/348 (76%), Gaps = 2/348 (0%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK++ S I K L LD GI+Y ++ +V+PNPTCKNV DG+A L GCD++IS+GG Sbjct: 36 TDKYIEGSDILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGG 95 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSPQD A I I+ATNGG +DYEG+HKS K LP+VA+NTTAGTSAE+TINYVITDEER Sbjct: 96 GSPQDAASCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEER 155 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 KVKMVMVDKNS+A ISVNDPELM+ KP LTAATGMDALTHA+EALVTPGAY VT ++ Sbjct: 156 KVKMVMVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSI 215 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI L R V NGHDIEARE MV AI YVH+MAHQLG VY+LPH Sbjct: 216 GAIELIKEYLPRAVANGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPH 275 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVC AMLLP +E ENAK VPE FR++AKA+GL +EGKSD ECAD+ I I+ LS+ VGIP Sbjct: 276 GVCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGIP 335 Query: 380 SKLSELGVTEVDLDL--LAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 KL+ELG+ E D D L++N++ DACAPGNPF+PT EE I ++++ Sbjct: 336 KKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF 383 >ref|NP_561365.1| alcohol dehydrogenase [Clostridium perfringens] dbj|BAB80155.1| alcohol dehydrogenase [Clostridium perfringens str. 13] Length = 382 Score = 368 bits (944), Expect = e-100, Method: Compositional matrix adjust. Identities = 188/346 (54%), Positives = 236/346 (68%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK L + GI K+ VLD I+YA+YDE KPNPT KNV+DGLA L + CD++IS+GG Sbjct: 37 TDKVLGQIGIVKKVTDVLDNKNIEYAIYDETKPNPTVKNVNDGLALLKEKECDFVISLGG 96 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GS D AK I ++ATNGG IKDYEGV KSK LP+V +NTTAGT +E+T+ +ITDEER Sbjct: 97 GSAHDCAKGIALLATNGGEIKDYEGVDKSKKPQLPMVGINTTAGTGSEMTLFAIITDEER 156 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 +KM +VDK+ I+VNDP LM+ P +LTAATGMDALTHAIEA V+ A P+TDA A Sbjct: 157 HIKMALVDKHLTPIIAVNDPMLMLAMPKSLTAATGMDALTHAIEAYVSTSATPITDACAE 216 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI L V+NG ++EAR+ M YA YVHAMAHQLGG Y+LPH Sbjct: 217 KAIELISNYLVNAVENGENVEARDMMAYAEYLAGMAFNNASLGYVHAMAHQLGGFYNLPH 276 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNA+LLP+V+E N + IAK +G IEG +D + AD I+ IK+LS+ +GIP Sbjct: 277 GVCNAILLPHVQEYNKDTSASRLAKIAKIMGGNIEGLTDEQGADLCIDMIKSLSQTIGIP 336 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 L LGV E D + LA N++ DAC+ NP EEVI +FRK + Sbjct: 337 EGLGVLGVKESDFETLATNALNDACSLTNPRKGNLEEVIAIFRKAM 382 >ref|NP_936583.1| alcohol dehydrogenase, class IV [Vibrio vulnificus YJ016] dbj|BAC96553.1| alcohol dehydrogenase, class IV [Vibrio vulnificus YJ016] Length = 382 Score = 363 bits (932), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 181/346 (52%), Positives = 241/346 (69%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK L + G+ ++ +L E ++ ++D +PNPT NV+ GLA L CD++IS+GG Sbjct: 37 TDKILNQIGVVKQVQDLLSERHVEAVVFDGTQPNPTIGNVNAGLALLKQNECDFVISLGG 96 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSP D AK I ++A NGG I DYEGV KS LP++A+NTTAGT++E+T +ITDEER Sbjct: 97 GSPHDCAKGIALVAANGGEIADYEGVDKSAKPMLPLIAINTTAGTASEMTRFCIITDEER 156 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 +KM +VDK++ ISVNDPELM+ KPA+LTAATGMDALTHA+EA V+ A P+TDA A+ Sbjct: 157 HIKMAIVDKHTTPLISVNDPELMLAKPASLTAATGMDALTHAVEAYVSIAATPITDAVAI 216 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI A+L V +G DIEAREQM YA YVHAMAHQLGG YDLPH Sbjct: 217 KAIELIQAHLRTAVAHGDDIEAREQMAYAQFMAGMAFNNASLGYVHAMAHQLGGFYDLPH 276 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNA+LLP+V+ NA+ PE+ R +AKA+G+ +EG S + A+ I++I AL+K+VGIP Sbjct: 277 GVCNAILLPHVQRYNAQVCPERLRDVAKAMGVNVEGMSAEQGAEAAIDAIVALAKDVGIP 336 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 + + ELG D+ LA+N++KDAC NP T EE+ +F + Sbjct: 337 AGIRELGAKSEDIPTLADNALKDACGFTNPKQATHEEISAIFEAAM 382 >ref|NP_762008.1| Alcohol dehydrogenase, class IV [Vibrio vulnificus CMCP6] gb|AAO06998.1| Alcohol dehydrogenase, class IV [Vibrio vulnificus CMCP6] Length = 370 Score = 363 bits (932), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 181/346 (52%), Positives = 241/346 (69%) Query: 80 TDKFLVKSGIAGKLLAVLDEAGIQYALYDEVKPNPTCKNVHDGLAFLNAQGCDYLISIGG 139 TDK L + G+ ++ +L E ++ ++D +PNPT NV+ GLA L CD++IS+GG Sbjct: 25 TDKILNQIGVVKQVQDLLSERHVETVVFDGTQPNPTIGNVNAGLALLKQNECDFVISLGG 84 Query: 140 GSPQDTAKAIGIIATNGGHIKDYEGVHKSKNKSLPIVAVNTTAGTSAEVTINYVITDEER 199 GSP D AK I ++A NGG I DYEGV KS LP++A+NTTAGT++E+T +ITDEER Sbjct: 85 GSPHDCAKGIALVAANGGEIADYEGVDKSAKPMLPLIAINTTAGTASEMTRFCIITDEER 144 Query: 200 KVKMVMVDKNSIATISVNDPELMIDKPAALTAATGMDALTHAIEALVTPGAYPVTDATAL 259 +KM +VDK++ ISVNDPELM+ KPA+LTAATGMDALTHA+EA V+ A P+TDA A+ Sbjct: 145 HIKMAIVDKHTTPLISVNDPELMLAKPASLTAATGMDALTHAVEAYVSIAATPITDAVAI 204 Query: 260 AAVELIFANLARTVKNGHDIEAREQMVYAIXXXXXXXXXXXXXYVHAMAHQLGGVYDLPH 319 A+ELI A+L V +G DIEAREQM YA YVHAMAHQLGG YDLPH Sbjct: 205 KAIELIQAHLRTAVAHGDDIEAREQMAYAQFMAGMAFNNASLGYVHAMAHQLGGFYDLPH 264 Query: 320 GVCNAMLLPYVEEENAKHVPEKFRSIAKAIGLQIEGKSDLECADFVIESIKALSKEVGIP 379 GVCNA+LLP+V+ NA+ PE+ R +AKA+G+ +EG S + A+ I++I AL+K+VGIP Sbjct: 265 GVCNAILLPHVQRYNAQVCPERLRDVAKAMGVNVEGMSAEQGAEAAIDAIVALAKDVGIP 324 Query: 380 SKLSELGVTEVDLDLLAENSMKDACAPGNPFIPTKEEVITLFRKIL 425 + + ELG D+ LA+N++KDAC NP T EE+ +F + Sbjct: 325 AGIRELGAKSEDIPTLADNALKDACGFTNPKQATHEEISAIFEAAM 370 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 590,719,439 Number of extensions: 22986145 Number of successful extensions: 66452 Number of sequences better than 1.0e-05: 963 Number of HSP's gapped: 64599 Number of HSP's successfully gapped: 963 Length of query: 425 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 297 Effective length of database: 409,704,906 Effective search space: 121682357082 Effective search space used: 121682357082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)