BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0045.p (340 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060249.1| COG0502: Biotin synthase and related enzymes ... 364 2e-99 ref|NP_810748.1| biotin synthase [Bacteroides thetaiotaomicron V... 316 4e-85 ref|NP_622716.1| Biotin synthase and related enzymes [Thermoanae... 307 2e-82 ref|NP_563376.1| probable biotin synthetase [Clostridium perfrin... 292 9e-78 ref|NP_348258.1| Biotin synthase family enzyme [Clostridium acet... 282 7e-75 >ref|ZP_00060249.1| COG0502: Biotin synthase and related enzymes [Clostridium thermocellum ATCC 27405] Length = 353 Score = 364 bits (934), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 172/332 (51%), Positives = 225/332 (67%), Gaps = 1/332 (0%) Query: 1 VSQQNLADLISHLEGALRRRLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRS 60 +S + LI H L L++ A + RI YG VY+RGLIEF+N C+ +C YCG+R Sbjct: 20 LSYDEMYQLIEHRNEELANYLFEKARQVRILYYGHDVYMRGLIEFTNYCRNDCYYCGIRK 79 Query: 61 GNRTLSRYRLQPEEILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAA 120 N RYRL E+IL+CC GY LG+RTFVLQ GED +YT L +++S+K ++PD A Sbjct: 80 SNCNAERYRLTKEQILECCDVGYELGFRTFVLQGGEDGYYTDKILADIVSSIKAKYPDCA 139 Query: 121 LTLSVGERDDESYTRLYAAGADRFLLRHETASASLYRALHP-TMEYXXXXXXXXXXQRIG 179 +TLS+GE+ ESY LY AGADR+LLRHETA+A Y LHP M + IG Sbjct: 140 ITLSLGEKSYESYKLLYEAGADRYLLRHETANAQHYSKLHPPVMSLKNRKQCLYNLKEIG 199 Query: 180 YQVGAGFMVGLPGQSXXXXXXXXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLD 239 YQVG GFMVG P Q+ +++ELQP M+GIGPF+PH+DTP + GT+E TL Sbjct: 200 YQVGCGFMVGSPFQTTECLVDDLMFIKELQPHMVGIGPFIPHKDTPFAGKPAGTLELTLF 259 Query: 240 MIALARLMVPDALIPATTAMGTAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKIC 299 ++ + RLM+P L+PATTA+GT HP GRE + AGANV+MPNLSP VR+KY+LY+NKIC Sbjct: 260 LLGIIRLMLPYVLLPATTALGTIHPKGRELGILAGANVVMPNLSPKEVRSKYLLYDNKIC 319 Query: 300 TGDESAQCRFCLEQRVKSAGFHVDMGRGDSLK 331 TGDE+A+CR CL R++S G+ + + RGD K Sbjct: 320 TGDEAAECRMCLTHRIESIGYKLVVSRGDCKK 351 >ref|NP_810748.1| biotin synthase [Bacteroides thetaiotaomicron VPI-5482] gb|AAO76942.1| biotin synthase [Bacteroides thetaiotaomicron VPI-5482] Length = 345 Score = 316 bits (810), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 150/326 (46%), Positives = 210/326 (64%), Gaps = 1/326 (0%) Query: 4 QNLADLISHLEGALRRRLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNR 63 + L++ +G R + A + + +G +++RGLIE SN C+ NC YCG+R GN Sbjct: 17 EEFRQLLTECDGESLRYINKQAQEVSLRHFGNRIFIRGLIEVSNCCRNNCYYCGIRKGNP 76 Query: 64 TLSRYRLQPEEILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAALTL 123 L RYRL E IL+CC++GY LG+RTFVLQ GED T +++ +++++++ +PD A+TL Sbjct: 77 NLERYRLSTENILNCCKQGYGLGFRTFVLQGGEDPALTEERIEDIVSTIRRSYPDCAITL 136 Query: 124 SVGERDDESYTRLYAAGADRFLLRHETASASLYRALHPT-MEYXXXXXXXXXXQRIGYQV 182 S+GE+ E+Y R + AGA+R+LLRHET Y+ LHP M + IGYQ Sbjct: 137 SLGEKSREAYERFFQAGANRYLLRHETYDKEHYQQLHPAGMSCEHRLQCLRDLKDIGYQT 196 Query: 183 GAGFMVGLPGQSXXXXXXXXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMIA 242 G G MVG PGQ+ ++++L+P+MIGIGPFL HRDTP GTVE TL +++ Sbjct: 197 GTGIMVGSPGQTIEHLIQDILFIEQLRPEMIGIGPFLSHRDTPFAQSPSGTVERTLLLLS 256 Query: 243 LARLMVPDALIPATTAMGTAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICTGD 302 + RLM P ALIPATTA+ T P+GRE+ + AGANV+MPNLSP R KY LYNNK G Sbjct: 257 IFRLMHPSALIPATTALATLTPDGREQGILAGANVVMPNLSPQEERKKYNLYNNKASLGA 316 Query: 303 ESAQCRFCLEQRVKSAGFHVDMGRGD 328 ESA+ L+Q+++ G+ + RGD Sbjct: 317 ESAEGLNILQQQLEKIGYQISFSRGD 342 >ref|NP_622716.1| Biotin synthase and related enzymes [Thermoanaerobacter tengcongensis] gb|AAM24320.1| Biotin synthase and related enzymes [Thermoanaerobacter tengcongensis] Length = 351 Score = 307 bits (786), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 147/323 (45%), Positives = 207/323 (64%), Gaps = 2/323 (0%) Query: 14 EGALRRRLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNRTLSRYRLQPE 73 EG + L+ A + R G V+LRGLIEFS+ CK +C YCGLR N+ RYR+Q + Sbjct: 28 EGEDKDLLFKTADRVRKEHVGDEVFLRGLIEFSSYCKNDCFYCGLRRSNQNAQRYRMQED 87 Query: 74 EILDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAALTLSVGERDDESY 133 EI++ + Y +GYRT VLQSGED +YT D+L +I +K+ D A+TLS+GER + Y Sbjct: 88 EIVEVAKRAYQMGYRTVVLQSGEDMYYTKDRLCSIIKKMKR-VVDVAITLSIGERSYDEY 146 Query: 134 TRLYAAGADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGYQVGAGFMVGLPGQ 193 AGADRFL+R ET++ LYR HP M + +++GY++G GF++GLPGQ Sbjct: 147 KVFKDAGADRFLMRFETSNEKLYRKYHPGMSFENRIECLKWLKKLGYELGTGFLIGLPGQ 206 Query: 194 SXXXXXXXXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMIALARLMVPDALI 253 + ++EL DMIGIGPF+PH TPL+D + G+V+ TL IA+ RL+ PDA I Sbjct: 207 TLDDLAQDIILVKELDADMIGIGPFIPHPQTPLKDAKEGSVDLTLKSIAILRLLNPDANI 266 Query: 254 PATTAMGTAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICTGDESAQCRFCLEQ 313 PATTA+GT P GR++ L GAN++MPN++ + + KY LY KIC +++ +CR C+E Sbjct: 267 PATTALGTLDPLGRQKGLMCGANIVMPNVNRLEYKLKYELYPGKICINEDATKCRGCIES 326 Query: 314 RVKSAGFHVDMGRGDSLKHLSRS 336 + S G V G+G S +H R+ Sbjct: 327 IIVSVGRKVGQGKGQS-RHYKRA 348 >ref|NP_563376.1| probable biotin synthetase [Clostridium perfringens] dbj|BAB82166.1| probable biotin synthetase [Clostridium perfringens str. 13] Length = 347 Score = 292 bits (747), Expect = 9e-78, Method: Compositional matrix adjust. Identities = 147/312 (47%), Positives = 194/312 (62%), Gaps = 2/312 (0%) Query: 16 ALRRRLYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNRTLSRYRLQPEEI 75 ++ L+ A + R G V+LRGLIEF+NICK+NC+YCGLR N+ L+RYRL EEI Sbjct: 27 SINEELFKAADEVRKKYLGDEVHLRGLIEFTNICKRNCMYCGLRRDNKNLNRYRLSHEEI 86 Query: 76 LDCCREGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAALTLSVGERDDESYTR 135 +D ++ GY+T VLQ GEDD+YT ++LV ++ +K ALTLS+GER E Y Sbjct: 87 IDFAKKAVGYGYKTLVLQGGEDDYYTVERLVPIVKDLKAL--GVALTLSIGERPFEEYEA 144 Query: 136 LYAAGADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGYQVGAGFMVGLPGQSX 195 L AGADRFLLR ET LY L P M + +++GY+VG+G +VGLPGQ Sbjct: 145 LKKAGADRFLLRIETTDRELYEELDPGMSHENRIQCLKNLRKLGYEVGSGCLVGLPGQKI 204 Query: 196 XXXXXXXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMIALARLMVPDALIPA 255 + +EL DM GIGPF+P+ DTPL+D + G E L ++A+ RL++PD IPA Sbjct: 205 ESLADDILFFKELDVDMNGIGPFIPNEDTPLKDAEGGQFELALKVMAIVRLLLPDINIPA 264 Query: 256 TTAMGTAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICTGDESAQCRFCLEQRV 315 TTAM T + GR ALQ GANV+MPN++ R Y LY KICTGD A CR C+ ++ Sbjct: 265 TTAMETLNKQGRVIALQCGANVVMPNVTEGEYRKLYALYPGKICTGDTPAHCRGCISGKI 324 Query: 316 KSAGFHVDMGRG 327 + G V G G Sbjct: 325 RGIGRIVSDGPG 336 >ref|NP_348258.1| Biotin synthase family enzyme [Clostridium acetobutylicum] pir||C97101 biotin synthase family enzyme [imported] - Clostridium acetobutylicum gb|AAK79598.1| Biotin synthase family enzyme [Clostridium acetobutylicum] Length = 350 Score = 282 bits (722), Expect = 7e-75, Method: Compositional matrix adjust. Identities = 134/317 (42%), Positives = 193/317 (60%), Gaps = 2/317 (0%) Query: 21 LYDLAHKARITAYGTAVYLRGLIEFSNICKQNCLYCGLRSGNRTLSRYRLQPEEILDCCR 80 +Y A + R G V+LRGLIEFSNICK+NC+YCGLR N+ + RYRL+P+EI+ + Sbjct: 35 IYKAADRVREKYVGEEVHLRGLIEFSNICKRNCMYCGLRRDNKNIKRYRLEPDEIIHLAK 94 Query: 81 EGYTLGYRTFVLQSGEDDWYTTDKLVQLITSVKQQFPDAALTLSVGERDDESYTRLYAAG 140 GY+T VLQSGEDD+YT +K+ +++ +K+ + A+TLS+GE+ E Y +G Sbjct: 95 SAKNYGYQTVVLQSGEDDYYTVEKMKYIVSEIKKL--NMAITLSIGEKTFEEYEEYRKSG 152 Query: 141 ADRFLLRHETASASLYRALHPTMEYXXXXXXXXXXQRIGYQVGAGFMVGLPGQSXXXXXX 200 ADR+L+R ET LY L P M + +++GY+VG+G +VGLP Q+ Sbjct: 153 ADRYLIRIETTDKELYEKLDPKMSHENRINCLKNLRKLGYEVGSGCLVGLPNQTIESLAD 212 Query: 201 XXXYLQELQPDMIGIGPFLPHRDTPLRDEQPGTVENTLDMIALARLMVPDALIPATTAMG 260 + +E+ DMIG+GPF+P+ DTPL +E+ G ++ + AL RL++PD IPATTAM Sbjct: 213 DILFFKEIDADMIGVGPFIPNEDTPLGEEKGGEFFMSVKVTALIRLLLPDINIPATTAME 272 Query: 261 TAHPNGRERALQAGANVLMPNLSPMSVRAKYMLYNNKICTGDESAQCRFCLEQRVKSAGF 320 + +PNGR AL +GANV+MPN++ R Y LY KIC D CR C+ ++ Sbjct: 273 SLYPNGRSIALTSGANVVMPNVTEGEYRKLYALYPGKICVNDTPGHCRQCISLKINKINR 332 Query: 321 HVDMGRGDSLKHLSRSL 337 V +G K S+ Sbjct: 333 KVSATKGFRKKSYKESI 349 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 457,949,304 Number of extensions: 17361438 Number of successful extensions: 34733 Number of sequences better than 1.0e-05: 63 Number of HSP's gapped: 34762 Number of HSP's successfully gapped: 63 Length of query: 340 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 214 Effective length of database: 415,135,104 Effective search space: 88838912256 Effective search space used: 88838912256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)