BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0504.0031.p (527 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_244550.1| xylan beta-1,4-xylosidase [Bacillus halodurans]... 578 e-164 ref|ZP_00056973.1| COG3507: Beta-xylosidase [Thermobifida fusca] 502 e-140 gb|AAB97967.1| xylosidase/arabinosidase [Selenomonas ruminantium] 459 e-128 gb|AAF66622.1| xylosidase/arabinofuranosidase [Azospirillum irak... 456 e-127 ref|NP_694047.1| xylan 1,4-beta-xylosidase [Oceanobacillus iheye... 443 e-123 >ref|NP_244550.1| xylan beta-1,4-xylosidase [Bacillus halodurans] pir||C84110 xylan beta-1,4-xylosidase xynB [imported] - Bacillus halodurans (strain C-125) dbj|BAB07402.1| xylan beta-1,4-xylosidase [Bacillus halodurans] Length = 528 Score = 578 bits (1491), Expect = e-164, Method: Compositional matrix adjust. Identities = 283/526 (53%), Positives = 354/526 (67%), Gaps = 3/526 (0%) Query: 1 MAPTIVNPVLKGFHPDPSFLRVGDDYYIATSTFEWFPGVEIHHSRDLVHWRPLTRVLTES 60 M I NP+L GFHPDPS +RVGDDYYIATSTFEWFPGV IHHSRDL HWR ++ LT + Sbjct: 1 MVNRIQNPILPGFHPDPSIVRVGDDYYIATSTFEWFPGVRIHHSRDLKHWRFVSSPLTRT 60 Query: 61 SQVDLRGNSSSGSIWAPALSYDNGIYYLLFTDVKSRKSAYKDLHNYLITAADILGPWSDP 120 SQ+D++GN +SG IWAP LSY +G +YL++TDVK A+KD HNYL+TA +I GPWSDP Sbjct: 61 SQLDMKGNMNSGGIWAPCLSYHDGTFYLIYTDVKQWHGAFKDAHNYLVTAQNIEGPWSDP 120 Query: 121 VRLNGSGFDPFLFHDADGTQWLLNMRWDFRKQHSNFSGIIMQQYDPKSRSLTGPVHELYK 180 + LN SGFDP LFHD DG +WL+NM WD+RK + F+GII+Q+Y + L GPV +YK Sbjct: 121 IYLNSSGFDPSLFHDDDGRKWLVNMIWDYRKGNHPFAGIILQEYSEAEQKLVGPVKNIYK 180 Query: 181 GTPAGVTEGPQLYQRNGYYYLLVAEGGTGINHMVTLARSRSRTGPYETDPDYPVMTTAHD 240 GT +TEGP LY+++GYYYLLVAEGGT H TLARS+S GPYETDP YP++T+ Sbjct: 181 GTDIQLTEGPHLYKKDGYYYLLVAEGGTEYEHAATLARSQSIDGPYETDPSYPLVTSTGQ 240 Query: 241 LAFAFQQAGHGSLVETQSGEWYMAHLCTRPIPGTGTMNPLGRETAIQRVVWTDDGWLRLA 300 A Q+AGHGSLVETQ+GEWY+AHLC RP+ G LGRETAIQ+V WT+DGWLR+ Sbjct: 241 PELALQKAGHGSLVETQNGEWYLAHLCGRPL--KGKYCTLGRETAIQKVNWTEDGWLRIE 298 Query: 301 HGGKLPALAVEGPDLEPHPFPEWPERDDFDYDTLGYPYQSLRVPFDPSWVSLTERPGFLR 360 GG P V PDL HPF + PE DDFD L + + +LR+P DPSW SL ERPG LR Sbjct: 299 DGGNHPLREVTAPDLPEHPFEKEPELDDFDAPQLHHQWNTLRIPADPSWCSLEERPGHLR 358 Query: 361 LRGRESLASLHDQSLIGRPIQHFACTVSTCLEFEPDTFLQMAGLVLXXXXXXXXXLRVTA 420 LRG ESL S+H QSL+ R Q F C V T LE++P++F MAGLV+ L VT Sbjct: 359 LRGMESLTSVHSQSLVARRQQSFHCEVETKLEYQPESFQHMAGLVIYYDTEDHVYLHVTW 418 Query: 421 DEIRGVSLGVVMCRAGKYDEITSMQISVKAWDRYYLKAAIDGRDIMFYASPDGEI-WTAM 479 E +G L ++ + G YDE+ + I + YLK I + Y +GE W + Sbjct: 419 HEEKGKCLQIIQTKGGNYDELLASPIPLAEEKAVYLKGRIHRETMHLYFKQEGEAEWQPV 478 Query: 480 CTPLDFGTLSDEYGGKLGFTGAYAGICVQDLDQQAKKAYFDYFEYR 525 +D +SD+ ++ FTG + G+ QDL K A FDYF Y+ Sbjct: 479 GPTIDVTHMSDDSAKQVRFTGTFVGMATQDLSGTKKPADFDYFRYK 524 >ref|ZP_00056973.1| COG3507: Beta-xylosidase [Thermobifida fusca] Length = 550 Score = 502 bits (1292), Expect = e-140, Method: Compositional matrix adjust. Identities = 265/533 (49%), Positives = 334/533 (62%), Gaps = 13/533 (2%) Query: 2 APTIVNPVLKGFHPDPSFLRVGDDYYIATSTFEWFPGVEIHHSRDLVHWRPLTRVLTESS 61 A TI NPVL GF+PDPS LRVGDDYY+ATSTFEW+PGV +HHSRDLVHWRPL LTE+ Sbjct: 17 AGTIRNPVLTGFYPDPSILRVGDDYYMATSTFEWYPGVTLHHSRDLVHWRPLGGALTETR 76 Query: 62 QVDLRGNSSSGSIWAPALSYDNGIYYLLFTDVKSRKSAYKDLHNYLITAADILGPWSDPV 121 +DL G +WAPALSY +G+++L+FT+V S + D NY+ TA DI GPWSDPV Sbjct: 77 LLDLAGRRDGAGVWAPALSYRDGLFFLVFTNVASYSGNFWDAPNYVTTAPDITGPWSDPV 136 Query: 122 RLNGSGFDPFLFHDADGTQWLLNMRWDFRKQHSNFSGIIMQQYDPKSRSLTGPVHELYKG 181 L+ GFDP LFHD DG WLL+ D+R F GI+ Q++ + L G ++ G Sbjct: 137 PLHSLGFDPSLFHDDDGRSWLLSTSMDWRPGRDAFGGIVAQEFSVRDMKLVGEPVIIFTG 196 Query: 182 TPAGVTEGPQLYQRNGYYYLLVAEGGTGINHMVTLARSRSRTGPYETDPDYPVMTTAHDL 241 T AGVTE P +Y+R+G+YYL+ AEGGT H VT+ARSRS TGPYE DP P +T+ H Sbjct: 197 TEAGVTEAPHIYKRDGWYYLVTAEGGTQWEHQVTVARSRSVTGPYEVDPAGPALTSRHVP 256 Query: 242 AFAFQQAGHGSLVETQSGEWYMAHLCTRPIPGTGTMNPLGRETAIQRVVWTDDGWLRLAH 301 Q+AGH S+VETQ GEWY AHL RP+P +G LGRETA+Q++ W+ DGW R+ + Sbjct: 257 EAPLQKAGHASMVETQHGEWYFAHLTGRPMPPSGRCV-LGRETALQKIEWSSDGWPRVRN 315 Query: 302 GGKLPALAVEGP-DLEPHPFPEWPERDDFDYDTLGYPYQSLRVPFDPSWVSLTERPGFLR 360 P L V GP L PHP+P+ E D FD T + +LR PFD SWVSLTERPG+LR Sbjct: 316 AE--PLLEVPGPRGLAPHPWPQPSETDHFDDPTPRPEWSTLRRPFDSSWVSLTERPGYLR 373 Query: 361 LRGRESLASLHDQSLIGRPIQHFACTVSTCLEFEPDTFLQMAGLVLXXXXXXXXXLRVTA 420 +RG +S A LH+ SL+ R +QH AC CLEF+P+ F QMAG+ LR+ Sbjct: 374 IRGGQSPAGLHEPSLVARRLQHRACIFEACLEFKPEDFRQMAGITAYYNTRQWHYLRINR 433 Query: 421 DEIRGVSLGVVMCRAGKYDEITSMQISVKAWDRYYLKAAIDGRDIMFYASPDGEIWTAMC 480 D+ GV GV+ G E+ +ISV W + +L+A ID D+ F S DG W M Sbjct: 434 DDRGGVFAGVLTSDRGIIREV-GRRISVTDWPKVFLRAEIDRNDLRFAVSSDGSTWADMG 492 Query: 481 TPLDFGTLSDEYGGK--------LGFTGAYAGICVQDLDQQAKKAYFDYFEYR 525 LD LSDEY + GFTGA+ G+ D+ A FD+ YR Sbjct: 493 VRLDMSILSDEYAEERFGNDPIMWGFTGAFLGLWAHDMTGAGLPADFDFCTYR 545 >gb|AAB97967.1| xylosidase/arabinosidase [Selenomonas ruminantium] Length = 538 Score = 459 bits (1181), Expect = e-128, Method: Compositional matrix adjust. Identities = 251/537 (46%), Positives = 322/537 (59%), Gaps = 18/537 (3%) Query: 4 TIVNPVLKGFHPDPSFLRVGDDYYIATSTFEWFPGVEIHHSRDLVHWRPLTRVLTESSQV 63 I NPVLKGF+PDPS +R GDDYYIATSTFEWFPGV+IHHS+DLVHW + L+ + + Sbjct: 2 NIQNPVLKGFNPDPSIVRAGDDYYIATSTFEWFPGVQIHHSKDLVHWHLVAHPLSTTEFL 61 Query: 64 DLRGNSSSGSIWAPALSYDNGIYYLLFTDVKSRKSAYKDLHNYLITAADILGPWSDPVRL 123 D++GN SG IWAP LSY +G ++L++TDVK +KD HNYL TA DI GPWS P+ L Sbjct: 62 DMKGNPDSGGIWAPDLSYADGKFWLIYTDVKVVDGMWKDCHNYLTTAEDIKGPWSKPILL 121 Query: 124 NGSGFDPFLFHDADGTQWLLNMRWDFRKQHSNFSGIIMQQYDPKSRSLTGPVHELYKGTP 183 NG+GFD LFHD G ++L+NM WD R H NF GI +Q+Y L G +YKGT Sbjct: 122 NGAGFDASLFHDPSGKKYLVNMYWDQRVYHHNFYGIALQEYSVAEEKLIGKPEIIYKGTD 181 Query: 184 AGVTEGPQLYQRNGYYYLLVAEGGTGINHMVTLARSRSRTGPYETDPDYPVMTTAHDLAF 243 TEGP LY N YYL+ AEGGT H T+ARS++ GPYE PDYP+++ ++ Sbjct: 182 IAYTEGPHLYYINDMYYLMTAEGGTTYQHSETIARSKTIHGPYEIQPDYPLLSAWKEVHN 241 Query: 244 AFQQAGHGSLVETQSGEWYMAHLCTRPIPGTG----------TMNPLGRETAIQRVVWTD 293 Q+ GH SLVETQ+G+WY+AHL RP+P PLGRETAIQ++ W Sbjct: 242 PLQKCGHASLVETQNGQWYLAHLTGRPLPAPAGFPSREREQHAFCPLGRETAIQKIEW-Q 300 Query: 294 DGWLRLAHGGKLPALAVEGPDLEPHPF-PEWPERDDFDYDTLGYPYQSLRVPFDPSWVSL 352 DGW + GG+ +L VE PDL + P + ERDDFD DTL +Q+LR+PF SL Sbjct: 301 DGWPVVV-GGQQGSLEVEAPDLPQQEWAPTYEERDDFDKDTLNINFQTLRIPFSEHLGSL 359 Query: 353 TERPGFLRLRGRESLASLHDQSLIGRPIQHFACTVSTCLEFEPDTFLQMAGLVLXXXXXX 412 T RPGFLRL GRESL S Q+ I R Q F T +EF P++F QMAGL Sbjct: 360 TARPGFLRLYGRESLQSKFTQAHIARRWQSFNFDAGTSVEFSPNSFQQMAGLTCYYNTEN 419 Query: 413 XXXLRVTADEIRGVSLGVVMCRAGKYD-EITSMQISV-KAWDRYYLKAAIDGRDIMFYAS 470 + VT +E +G + +V G + + +I + + K ++ GR + S Sbjct: 420 WSSIHVTWNEEKGRIIDLVTADNGTFSMPLAGAEIPIPDEVKTVHFKVSVRGRIYQYAYS 479 Query: 471 PDGEIWTAMCTPLDFGTLSDEY--GGKLGFTGAYAGICVQDLDQQAKKAYFDYFEYR 525 DGE + + L LSD+Y GG FTGA+ GI D+ A A FDYF Y+ Sbjct: 480 FDGETFHTLPIELPSWKLSDDYVRGGGF-FTGAFVGINAIDITGTALPADFDYFTYK 535 >gb|AAF66622.1| xylosidase/arabinofuranosidase [Azospirillum irakense] Length = 542 Score = 456 bits (1172), Expect = e-127, Method: Compositional matrix adjust. Identities = 250/534 (46%), Positives = 327/534 (61%), Gaps = 17/534 (3%) Query: 5 IVNPVLKGFHPDPSFLRVGDDYYIATSTFEWFPGVEIHHSRDLVHWRPLTRVLTESSQVD 64 I NP+L GF+PDPS +RVG DYYIATSTFEWFPGV+IHHSRDLVHWR LTR L SQ++ Sbjct: 2 IRNPILPGFNPDPSIVRVGADYYIATSTFEWFPGVQIHHSRDLVHWRLLTRPLRRESQLN 61 Query: 65 LRGNSSSGSIWAPALSYDNGIYYLLFTDVK---------SRKSAYKDLHNYLITAADILG 115 + G S +WAP L++ +G+++L++TDVK + ++ +D HNYL+T I G Sbjct: 62 MLGAPDSCGVWAPCLTHADGLFHLIYTDVKRYGRTSVGGASGASLRDFHNYLVTCDRIDG 121 Query: 116 PWSDPVRLNGSGFDPFLFHDADGTQWLLNMRWDFRKQHSNFSGIIMQQYDPKSRSLTGPV 175 WSDPV LN SGFDP LFHD DG ++LLNM WD R H+ F+GI++Q+Y P++R+L G Sbjct: 122 EWSDPVFLNSSGFDPSLFHDEDGRKYLLNMLWDHRPGHNRFAGIVLQEYSPQARALVGER 181 Query: 176 HELYKGTPAGVTEGPQLYQRNGYYYLLVAEGGTGINHMVTLARSRSRTGPYETDPDYPVM 235 ++ GT G+TE P LY+R+G+YYLL AEGGTG NH VT+ARSRS GPYE PD ++ Sbjct: 182 RVIFPGTALGLTEAPHLYKRDGWYYLLTAEGGTGWNHAVTMARSRSIDGPYELHPDTHIL 241 Query: 236 TTAHDLAFAFQQAGHGSLVETQSGEWYMAHLCTRPIPGTGTMNPLGRETAIQRVVWTDDG 295 T+ + Q+AGH LVET GE Y +LC RP+P G LGRETAIQR+VW DDG Sbjct: 242 TSFNRPDAPLQRAGHADLVETAEGETYAVYLCGRPLPNRGRCV-LGRETAIQRMVWGDDG 300 Query: 296 WLRLAHGGKLPALAVEGPDLEPHPFPEWPERDDFDYDTLGYPYQSLRVPFDPSWVSLTER 355 WLR G LP+L P L HPFP P R DFD +L +Q L P SL R Sbjct: 301 WLRTMDGQGLPSLTTPSPGLPAHPFPTAPARSDFDDPSLPLDFQWLPSPHPEELFSLFAR 360 Query: 356 PGFLRLRGRESLASLHDQSLIGRPIQHFACTVSTCLEFEPDTFLQMAGLVLXXXXXXXXX 415 PG+LRL GRE++ S Q+L+ R Q F +V+T ++F P F QMAGLV Sbjct: 361 PGWLRLYGRETIGSQFRQALVARRQQAFCVSVATFMDFHPRHFQQMAGLVCYYGGSKFHY 420 Query: 416 LRVTADEIRGVSLGVV--MCRAGKYDEITSMQISVKAWDRYYLKAAIDGRDIMFYASPDG 473 L V+ DE RG + V+ + + + D T M + + ++G + PDG Sbjct: 421 LYVSHDEARGRHIRVMSALPDSTQADAFTEMIPIPEGPIICGQSSTMNG--CFAFRLPDG 478 Query: 474 EIWTAMCTPLDFGTLSDEYG--GKLGFTGAYAGICVQDLDQQAKKAYFDYFEYR 525 + W + D LSDE G FTGA+ G+ QD+ A FD+F+Y+ Sbjct: 479 D-WVWLPRIFDASILSDEATSPGAPNFTGAFIGMACQDMAGSGVSADFDWFDYQ 531 >ref|NP_694047.1| xylan 1,4-beta-xylosidase [Oceanobacillus iheyensis HTE831] dbj|BAC15081.1| xylan 1,4-beta-xylosidase (beta xylosidase) (EC 3.2.1.37) [Oceanobacillus iheyensis HTE831] Length = 536 Score = 443 bits (1140), Expect = e-123, Method: Compositional matrix adjust. Identities = 236/534 (44%), Positives = 325/534 (60%), Gaps = 15/534 (2%) Query: 4 TIVNPVLKGFHPDPSFLRVGDDYYIATSTFEWFPGVEIHHSRDLVHWRPLTRVLTESSQV 63 I NP+L GF+PDPS R G+DYYIA STFEWFPGV I+HSRDL +W+ ++R L SQ+ Sbjct: 3 NIKNPILTGFNPDPSICRAGEDYYIAVSTFEWFPGVGIYHSRDLKNWKLVSRPLNRLSQL 62 Query: 64 DLRGNSSSGSIWAPALSYDNGIYYLLFTDVKSRKSAYKDLHNYLITAADILGPWSDPVRL 123 ++ GN +SG +WAPALSY +G ++L++TDVK +KD HNYL+T I G WS+P+ L Sbjct: 63 NMMGNPNSGGVWAPALSYKDGKFWLIYTDVKVTGGQWKDCHNYLVTCNSIDGEWSEPIYL 122 Query: 124 NGSGFDPFLFHDADGTQWLLNMRWDFRKQHSNFSGIIMQQYDPKSRSLTGPVHELYKGTP 183 N SGFDP LFHD DG ++L+NM WD R F GI++Q+YD L G ++KGT Sbjct: 123 NSSGFDPSLFHDEDGKKYLVNMVWDSRISTHPFYGIVLQEYDSVKGKLVGKKEIIFKGTD 182 Query: 184 AGVTEGPQLYQRNGYYYLLVAEGGTGINHMVTLARSRSRTGPYETDPDYPVMTTAHDLAF 243 +TE P LY+ N YYYLL AEGGT +H T+ARS++ GPYE PD P++++ Sbjct: 183 VKLTEAPHLYKINDYYYLLTAEGGTRYDHQATIARSKNLHGPYEVHPDNPLISSHSHPRN 242 Query: 244 AFQQAGHGSLVETQSGEWYMAHLCTRPIPGTGT-------MNPLGRETAIQRVVWTDDGW 296 Q+AGH S+V+T + EW+M HL RP+P PLGRETAIQR+ W ++ W Sbjct: 243 PLQKAGHASIVQTHTDEWFMVHLTGRPLPKENQPILDPRGYCPLGRETAIQRLEWKNE-W 301 Query: 297 LRLAHGGKLPALAVEGPDL-EPHPFPEWPERDDFDYDTLGYPYQSLRVPFDPSWVSLTER 355 + GG P++ +EGP + E + DDFD + L YQ+LR+P + + +LTER Sbjct: 302 PYVV-GGNQPSMEIEGPKIPESSADVQLSGIDDFDENELNLNYQTLRIPLNENIKTLTER 360 Query: 356 PGFLRLRGRESLASLHDQSLIGRPIQHFACTVSTCLEFEPDTFLQMAGLVLXXXXXXXXX 415 PG LRL GRESL S Q+ + R QHFA T T ++F+P +F Q AGLV Sbjct: 361 PGHLRLYGRESLTSTFTQAFVARRWQHFAFTAETKVDFQPTSFQQGAGLVNYYNTENWSA 420 Query: 416 LRVTADEIRGVSLGVVMCRAGKYDE-ITSMQISV-KAWDRYYLKAAIDGRDIMFYASPDG 473 L++T DE +G L + C K+++ + S +I + YLK ++ + ++ S +G Sbjct: 421 LQITWDEQKGKVLSLTTCDNFKFEQPLLSKEIEIPDEQGDVYLKVVVNYNNYQYFYSFNG 480 Query: 474 EIWTAMCTPLDFGTLSDEY--GGKLGFTGAYAGICVQDLDQQAKKAYFDYFEYR 525 W A+ L LSD+Y GG FTGA+ G+ QD + A FDYF Y+ Sbjct: 481 NDWIAIPITLQSYKLSDDYVKGGGF-FTGAFVGMQCQDTSGEGIHADFDYFYYQ 533 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.139 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 873,655,425 Number of extensions: 38921627 Number of successful extensions: 69423 Number of sequences better than 1.0e-05: 99 Number of HSP's gapped: 69075 Number of HSP's successfully gapped: 101 Length of query: 527 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 397 Effective length of database: 404,274,708 Effective search space: 160497059076 Effective search space used: 160497059076 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)