BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0500.0011.p (461 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00059019.1| COG0635: Coproporphyrinogen III oxidase and r... 315 1e-84 ref|NP_783061.1| putative oxygen-independent coproporphyrogen II... 312 1e-83 gb|AAM94763.1| CalE1 [Micromonospora echinospora] 312 1e-83 ref|NP_462892.1| putative coproporphyrinogen III oxidase [Salmon... 259 8e-68 ref|NP_747202.1| oxygen-independent coproporphyrinogen III oxida... 133 7e-30 >ref|ZP_00059019.1| COG0635: Coproporphyrinogen III oxidase and related Fe-S oxidoreductases [Thermobifida fusca] Length = 505 Score = 315 bits (808), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 184/430 (42%), Positives = 248/430 (57%), Gaps = 7/430 (1%) Query: 34 PYRNYLYSYPHKTAYRDLNPPVPLEPLWREEPAESFFLYMHIPFCGARCGFCNLFTLPDK 93 PYR Y+Y YPHKTAYR L P L +WR+EP ++ FLY HIPFC RCGFCNLFT Sbjct: 75 PYRTYVYGYPHKTAYRPLIPAPRLADVWRDEPTDALFLYFHIPFCEVRCGFCNLFTRAVP 134 Query: 94 RAEVHTVYVDALERQARQWAAFTGGKP-FARFAIGGGTPSLLAPAQLRRLFRIAEDIMGL 152 AE T Y+DAL RQA A GG FAR A GGGTP+ L +L RL+ +A+ ++G+ Sbjct: 135 PAEQVTAYLDALARQAEAVADALGGTARFARAAFGGGTPTYLTADELHRLYDLADRVLGV 194 Query: 153 DPASASISVETSPETLTEDKLNILKEHTVDRVSMGIQSFVAAESAAIYRPQNPDEVYRAL 212 D A+ SVETSP T T ++L+++ R+S+G+QSF+ AE+ A+ RPQ +V RAL Sbjct: 195 DLAAIPTSVETSPATATPERLDVVAARGASRISIGVQSFLDAEAHAVGRPQRRADVDRAL 254 Query: 213 ELLKLYDFPV-LNLDLIYGLPGQTVDSWLYSLEQAVNHEPEEIFIYPLYTRDHTIVKPED 271 ++ + LN+DLIYG+ GQT DSW YSL+ AV + PEEI++YPLY R T + Sbjct: 255 AAIRSHAPRARLNIDLIYGIAGQTPDSWRYSLDAAVAYRPEEIYLYPLYVRPLTGLGKRG 314 Query: 272 IRRQTDIRHECYRAAQQLLAEHGYTQYSMRRFARATAGTHKSIIDYSCQEEGMVGLGCGA 331 R D R Y+ + L E GY Q SMR F R A + Y CQ +GMVGLGCGA Sbjct: 315 -RAWDDQRRTLYQQGRDYLLERGYRQVSMRMFERVDAPSETGPA-YCCQTDGMVGLGCGA 372 Query: 332 RSYTRNVHYASRYGVSRKATESIIADYVA--AERYDTADYGIVLSLEEQKRRYILKAVLH 389 RSYTR +HY+ Y V + +I+ DY++ +D A+ G L +E++RR++L+++L Sbjct: 373 RSYTRGLHYSFDYAVGVRQVRAILDDYISRGPGGFDCAEVGFRLDEDERRRRHLLQSLLQ 432 Query: 390 SEGLKLADYSGRFGSSLWRDHPXXXXXXXXXX-XXXXXXXXXXXXXXXXYSDSIGDWFIS 448 ++GL L Y RF S D P YSD++G F S Sbjct: 433 ADGLDLDWYRSRFASDPRTDFPAEFAVLTQRGWVTETSRVVRLTDEGLAYSDTVGPLFFS 492 Query: 449 GEIREQMERF 458 + M + Sbjct: 493 PRVTRLMAEY 502 >ref|NP_783061.1| putative oxygen-independent coproporphyrogen III oxidase [Clostridium tetani E88] gb|AAO36998.1| putative oxygen-independent coproporphyrogen III oxidase [Clostridium tetani E88] Length = 439 Score = 312 bits (799), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 157/439 (35%), Positives = 246/439 (56%), Gaps = 5/439 (1%) Query: 23 VTQWREHITADPYRNYLYSYPHKTAYRDLNPPVPLEPLWREEPAESFFLYMHIPFCGARC 82 +T ++I +PY++Y+YSYPHK +YR + L+ LW +++ LY+HIPFC +C Sbjct: 1 MTDLEKYIEENPYKSYIYSYPHKKSYRTFDKAFDLKKLWENRKSDNLTLYIHIPFCLNKC 60 Query: 83 GFCNLFTLPDKRAEVHTVYVDALERQARQWAAFTGGKP---FARFAIGGGTPSLLAPAQL 139 G+CNLF+ D + + YV+ L + + + F GGGTP+ L + Sbjct: 61 GYCNLFSTTDFNKDKMSKYVNKLIEEIKAVKNILDLRKESVFYSVIFGGGTPTALPKDLM 120 Query: 140 RRLFRIAEDIMGLDPASASISVETSPETLTEDKLNILKEHTVDRVSMGIQSFVAAESAAI 199 +L + E + +D S+ETSP T+ ++ +++LK+ ++R+SMGIQSF E I Sbjct: 121 EKLLIVIEKDLNIDFKKIFFSMETSPNTMNKEYIDLLKKFHINRISMGIQSFKECELKEI 180 Query: 200 YRPQNPDEVYRALELLKLYDFPVLNLDLIYGLPGQTVDSWLYSLEQAVNHEPEEIFIYPL 259 R + + + ++LEL+K + + NLDLIYG+P Q+++S++ SL++ + + PEEIFIYPL Sbjct: 181 CRFETVNNINKSLELMKKENIEIKNLDLIYGIPSQSMESFMDSLKRVIEYRPEEIFIYPL 240 Query: 260 YTRDHTIVKPEDIRRQTDIRHECYRAAQQLLAEHGYTQYSMRRFARATAGTHKSIIDYSC 319 Y R+ T + E +R + Y A+ L ++GY Q SMR F R K YSC Sbjct: 241 YIREKTKL-YEKYKRDFNKMTNMYELAKDELIKNGYIQTSMRNFIREDMN-EKLYPSYSC 298 Query: 320 QEEGMVGLGCGARSYTRNVHYASRYGVSRKATESIIADYVAAERYDTADYGIVLSLEEQK 379 QE M+G+GCGARSY N+HY+ +Y V++ SII +Y+ E A YG +LS +E K Sbjct: 299 QENEMIGIGCGARSYISNIHYSIKYAVNQSNINSIINNYLKEENLYYATYGYILSEDEIK 358 Query: 380 RRYILKAVLHSEGLKLADYSGRFGSSLWRDHPXXXXXXXXXXXXXXXXXXXXXXXXXXYS 439 R+YILK++L GL L +Y RF +++ D YS Sbjct: 359 RKYILKSILKVTGLDLEEYFKRFNKNIFDDFNEINLLIDKGFLIKKGTRVYPTEKGLKYS 418 Query: 440 DSIGDWFISGEIREQMERF 458 D++G FIS E+ ++ + F Sbjct: 419 DAMGCLFISNEVNKKTQDF 437 >gb|AAM94763.1| CalE1 [Micromonospora echinospora] Length = 441 Score = 312 bits (799), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 182/435 (41%), Positives = 243/435 (55%), Gaps = 8/435 (1%) Query: 30 ITADPYRNYLYSYPHKTAYRDLNPPVPLEPLWREEPAESFFLYMHIPFCGARCGFCNLFT 89 + PY+ YLY+YPHKT+YR L P L +WR E ++ FLY+H+PFC RCGFCNLFT Sbjct: 8 LGGSPYQQYLYAYPHKTSYRPLRPRPLLADVWRAEARDALFLYVHVPFCEMRCGFCNLFT 67 Query: 90 LPDKRAEVHTVYVDALERQARQWA-AFTGGKPFARFAIGGGTPSLLAPAQLRRLFRIAED 148 + AE T Y+ L RQA Q A A +AR A GGGTP+ L +L LF IA Sbjct: 68 RANAPAEQVTGYLRQLRRQAEQVADALGADAGYARAAFGGGTPTYLTADELAELFDIATT 127 Query: 149 IMGLDPASASISVETSPETLTEDKLNILKEHTVDRVSMGIQSFVAAESAAIYRPQNPDEV 208 G +SVETSP T T D+L +L H RVS+G+QSF+ AE+ A RPQ EV Sbjct: 128 TTGARLPGVPLSVETSPATATPDRLAVLAAHGTTRVSIGVQSFLDAEARAAGRPQRRTEV 187 Query: 209 YRALELLKLYDFPVLNLDLIYGLPGQTVDSWLYSLEQAVNHEPEEIFIYPLYTRDHT-IV 267 AL ++ PVLN+DLIYG+ GQT D+W SL+ A+ PEE+++YPLY R T + Sbjct: 188 EAALGAIRDARIPVLNIDLIYGIDGQTADTWRESLDAALAWRPEELYLYPLYVRPLTGLG 247 Query: 268 KPEDIRRQTDI-RHECYRAAQQLLAEHGYTQYSMRRFARATAGTHKSIIDYSCQEEGMVG 326 + R + D R Y A + L GY Q SMR+F RA DY CQ++GMVG Sbjct: 248 RRAHHRAEWDAQRLALYEQAVEALDAAGYRQESMRQFRRADVPPPDG-PDYCCQDDGMVG 306 Query: 327 LGCGARSYTRNVHYASRYGVSRKATESIIADYVA--AERYDTADYGIVLSLEEQKRRYIL 384 LGCGARSYT ++HY+ Y VS +++ DY+A A+ + A++G L EQ+RR++L Sbjct: 307 LGCGARSYTTSLHYSFDYAVSVTEVRAVLDDYLARPADDFRHAEFGFTLDGAEQRRRWLL 366 Query: 385 KAVLHSEGLKLADYSGRFGSSLWRDHPXXXXXXXXXXXXXXXXXXXXXXXXXXYSDSIGD 444 K++L +G+ A Y RFG D P SD++G Sbjct: 367 KSLLRVDGVDPAAYRARFGRPPGDDFPELGRLVDRGWATDGGLRLTPAGLAR--SDAVGP 424 Query: 445 WFISGEIREQMERFV 459 W S +R+ M +V Sbjct: 425 WLTSARVRDAMTGYV 439 >ref|NP_462892.1| putative coproporphyrinogen III oxidase [Salmonella typhimurium LT2] gb|AAL22851.1| putative coproporphyrinogen III oxidase and related FeS oxidoreductase [Salmonella typhimurium LT2] Length = 413 Score = 259 bits (663), Expect = 8e-68, Method: Compositional matrix adjust. Identities = 161/427 (37%), Positives = 225/427 (52%), Gaps = 20/427 (4%) Query: 33 DPYRNYLYSYPHKTAYRDLNPPVPLEPLWREEPAESFFLYMHIPFCGARCGFCNLFTLPD 92 D YR Y+YSYPHKTAYR+L V + LY+H+PFC +RCGFCNLFT Sbjct: 5 DIYRQYMYSYPHKTAYRELEN-VSFSEVKPRIYEHDTHLYVHMPFCQSRCGFCNLFTCTG 63 Query: 93 KRAEVHTVYVDALERQARQWAAFTGGKPFARFAIGGGTPSLLAPAQLRRLFRIAEDIMGL 152 Y+DA+ Q RQ A +A F +GGGTP LL AQL +LF D++ + Sbjct: 64 ADNTFIDSYIDAIITQGRQMA--LAPAHWASFTVGGGTPLLLNIAQLEKLFFSVFDVLNV 121 Query: 153 DPASASISVETSPETLTEDKLNILKEHTVDRVSMGIQSFVAAESAAIYRPQNPDEVYRAL 212 D + ++ETSP T +K+ +L +V+RVS+G+QSF +E ++R N ++AL Sbjct: 122 D-WNIYKTIETSPTDTTAEKVALLNAFSVNRVSIGVQSFHDSELHTLHRRHNAASAHQAL 180 Query: 213 ELLKLYDFPVLNLDLIYGLPGQTVDSWLYSLEQAVNHEPEEIFIYPLYTRDHTIVKPEDI 272 E LK FP LN+D+IYG+PGQT S SL QA+ ++PEE+F+YPLY ++ Sbjct: 181 EWLKAGHFPSLNIDIIYGIPGQTHASLTESLHQALVYQPEELFLYPLY----------NM 230 Query: 273 RRQTDIRHECYRAAQQLLAEHGYTQYSMRRFARATAGTHKSIIDYSCQEEGMVGLGCGAR 332 RQ ++ H Y A+ LL GYTQ SMRRF A S SC E + LG G R Sbjct: 231 PRQENM-HSYYVLARDLLRNAGYTQTSMRRFVLNPA---PSAAAESCGFENSLALGAGGR 286 Query: 333 SYTRNVHYASRYGVSRKATESIIADYVAAERYDTADYGIVLSLEEQKRRYILKAVLHSEG 392 SY N+HY S + + + + II ++ + ++G +L +E KRR+I+K + +G Sbjct: 287 SYLGNLHYCSPWSSNLQTSRKIIQAFIDSPDKTVINHGYLLPPDEMKRRFIIKNLFFWQG 346 Query: 393 LKLADYSGRFGSSLWRDHPXXXXXXXXXXXXXXXXXXXXXXXXXXYSDSIGDWFISGEI- 451 L L+DY F S RD P SD + F+S E+ Sbjct: 347 LSLSDYQQYFASDALRDFPDLERFIRQGYCYQNGSRLRLTETGMALSDCLAPVFVSPEVM 406 Query: 452 -REQMER 457 RE +R Sbjct: 407 LRENRQR 413 >ref|NP_747202.1| oxygen-independent coproporphyrinogen III oxidase, putative [Pseudomonas putida KT2440] gb|AAN70666.1| oxygen-independent coproporphyrinogen III oxidase, putative [Pseudomonas putida KT2440] Length = 391 Score = 133 bits (335), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 97/338 (28%), Positives = 167/338 (49%), Gaps = 11/338 (3%) Query: 71 LYMHIPFCGARCGFCNLFTLPDKRAEVHTVYVDALERQARQWAAFTGGKPFARFAIGGGT 130 LY+HIP+C +C +C+ + YV AL Q A G+P + GGGT Sbjct: 21 LYIHIPWCVRKCPYCDFNSHAAGPELPEDAYVTALLDDLDQELAAVQGRPISSIFFGGGT 80 Query: 131 PSLLAPAQLRRLFRIAEDIMGLDPASASISVETSPETLTEDKLNILKEHTVDRVSMGIQS 190 PSL + + L RL R E + P I++E +P T +DK ++ ++R+S+G+QS Sbjct: 81 PSLFSASALGRLLRGVEQRIPFAP-DIEITLEANPGTFEQDKFKAYRQTGINRLSIGVQS 139 Query: 191 FVAAESAAIYRPQNPDEVYRALELLKLYDFPVLNLDLIYGLPGQTVDSWLYSLEQAVNHE 250 F A+ A+ R N DE RA + + F N+DL++GLP Q++D L L QA++ Sbjct: 140 FQPAKLEALGRIHNGDEAVRAAGMARAAGFDNFNMDLMHGLPDQSLDDALGDLRQAIDLG 199 Query: 251 PEEIFIYPLYTRDHTIV--KPEDIRRQTDIRHECYRAAQQLLAEHGYTQYSMRRFARATA 308 P + Y L +T+ +P ++ + DI + A Q L+AEHGY QY + +A+A Sbjct: 200 PTHLSWYQLTVEPNTVFWNQPPEL-PEDDILWDIQEAGQALMAEHGYRQYEVSAYAQAGR 258 Query: 309 GTHKSIIDYSCQEEGMVGLGCGARSYTRNVHYASRYGVSRKATESIIADYVAAERYDTAD 368 ++ + + +G+G GA + +A + R + DY+ + A Sbjct: 259 AARHNLNYWRFGD--FIGIGAGAHG---KLTFADGR-ILRTWKTRLPKDYLNLAKPFRAG 312 Query: 369 YGIVLSLEEQKRRYILKAVLHSEGLKLADYSGRFGSSL 406 + L ++E +++ A+ ++G++ ++ R G L Sbjct: 313 EKL-LPVDELPFEFLMNALRLTDGVEAELFTQRTGLPL 349 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.137 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 673,778,079 Number of extensions: 27456962 Number of successful extensions: 59936 Number of sequences better than 1.0e-05: 442 Number of HSP's gapped: 59045 Number of HSP's successfully gapped: 442 Length of query: 461 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 332 Effective length of database: 406,989,807 Effective search space: 135120615924 Effective search space used: 135120615924 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)