BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0497.0002.p (53 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_632643.1| conserved protein [Methanosarcina mazei Goe1] >... 90 1e-17 ref|NP_781617.1| thymidylate kinase [Clostridium tetani E88] >gi... 90 1e-17 ref|NP_391962.1| similar to hypothetical proteins [Bacillus subt... 90 2e-17 gb|AAH65526.1| FLJ21347 protein [Homo sapiens] 89 2e-17 ref|ZP_00062180.1| COG1331: Highly conserved protein containing ... 89 3e-17 >ref|NP_632643.1| conserved protein [Methanosarcina mazei Goe1] gb|AAM30315.1| conserved protein [Methanosarcina mazei Goe1] Length = 700 Score = 90.1 bits (222), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 39/50 (78%), Positives = 45/50 (90%) Query: 4 HKVPNRLAKEKSPYLLQHAHNPVNWFPWSEEAFEIAKRDNKPVFLSIGYS 53 K PNRL KEKSPYLLQHA+NPV+W+PW EEAFE A+++NKPVFLSIGYS Sbjct: 8 QKEPNRLIKEKSPYLLQHAYNPVDWYPWGEEAFEKARKENKPVFLSIGYS 57 >ref|NP_781617.1| thymidylate kinase [Clostridium tetani E88] gb|AAO35554.1| thymidylate kinase [Clostridium tetani E88] Length = 713 Score = 90.1 bits (222), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 37/51 (72%), Positives = 48/51 (94%) Query: 3 THKVPNRLAKEKSPYLLQHAHNPVNWFPWSEEAFEIAKRDNKPVFLSIGYS 53 +++VPNRLA+EKSPYLLQHA+NPV+W+PW EEAF+ AK ++KP+FLSIGYS Sbjct: 40 SNRVPNRLAQEKSPYLLQHAYNPVDWYPWGEEAFQKAKEEDKPIFLSIGYS 90 >ref|NP_391962.1| similar to hypothetical proteins [Bacillus subtilis] sp|P37512|YYAL_BACSU Hypothetical protein yyaL pir||S66006 conserved hypothetical protein yyaL - Bacillus subtilis dbj|BAA05212.1| unknown [Bacillus subtilis] emb|CAB16119.1| yyaL [Bacillus subtilis subsp. subtilis str. 168] Length = 689 Score = 89.7 bits (221), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 39/47 (82%), Positives = 42/47 (89%) Query: 7 PNRLAKEKSPYLLQHAHNPVNWFPWSEEAFEIAKRDNKPVFLSIGYS 53 PNRL EKSPYLLQHAHNPV+WFPW EEAFE AKR+NKPV +SIGYS Sbjct: 7 PNRLINEKSPYLLQHAHNPVDWFPWGEEAFEKAKRENKPVLVSIGYS 53 >gb|AAH65526.1| FLJ21347 protein [Homo sapiens] Length = 742 Score = 89.4 bits (220), Expect = 2e-17, Method: Composition-based stats. Identities = 36/52 (69%), Positives = 46/52 (88%) Query: 2 TTHKVPNRLAKEKSPYLLQHAHNPVNWFPWSEEAFEIAKRDNKPVFLSIGYS 53 T +VPNRL EKSPYLLQHA+NPV+W+PW EEAF+ A+++NKP+FLS+GYS Sbjct: 13 TPQRVPNRLIHEKSPYLLQHAYNPVDWYPWGEEAFDKARKENKPIFLSVGYS 64 >ref|ZP_00062180.1| COG1331: Highly conserved protein containing a thioredoxin domain [Clostridium thermocellum ATCC 27405] Length = 888 Score = 89.0 bits (219), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 38/53 (71%), Positives = 46/53 (86%) Query: 1 MTTHKVPNRLAKEKSPYLLQHAHNPVNWFPWSEEAFEIAKRDNKPVFLSIGYS 53 M+ +K NRL EKSPYLLQHA+NPV+W+PW +EAFE AKR+NKP+FLSIGYS Sbjct: 1 MSAYKQANRLIHEKSPYLLQHAYNPVDWYPWCDEAFEKAKRENKPIFLSIGYS 53 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.132 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 83,534,971 Number of extensions: 2345671 Number of successful extensions: 6074 Number of sequences better than 1.0e-05: 176 Number of HSP's gapped: 6073 Number of HSP's successfully gapped: 176 Length of query: 53 Length of database: 757,237,578 Length adjustment: 26 Effective length of query: 27 Effective length of database: 686,645,004 Effective search space: 18539415108 Effective search space used: 18539415108 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)