BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0495.0008.p (343 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO91750.1| sugar dehydrogenase; StmD [Rhizobium leguminosaru... 253 4e-66 ref|NP_461680.1| putative dehydrogenase [Salmonella typhimurium ... 174 4e-42 ref|NP_466322.1| similar to dehydrogenase [Listeria monocytogene... 148 1e-34 ref|NP_472259.1| similar to dehydrogenase [Listeria innocua] >gi... 148 2e-34 ref|NP_613535.1| Predicted dehydrogenase [Methanopyrus kandleri ... 135 1e-30 >gb|AAO91750.1| sugar dehydrogenase; StmD [Rhizobium leguminosarum bv. viciae] Length = 341 Score = 253 bits (646), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 135/342 (39%), Positives = 194/342 (56%), Gaps = 3/342 (0%) Query: 1 MTVIKMGIVGAGTWGETHAFIYNDHPGAEVTAVCDMNIERAKALADKFNIPGNHVFTDHK 60 M+ ++MG++GAG WG HA +N P E+ VCD++ RA + + + FTD++ Sbjct: 1 MSRLRMGVIGAGLWGGNHAHTFNVLPETELVGVCDLDEGRALKMKETYG--ATRAFTDYQ 58 Query: 61 EMLRSADIDAVAIVTPDFAHTRIAVDCANAGKHFIIEKPLTTSREEADEIVEAVERNNVR 120 +++ S IDA+++ TPDF HT I + A KH + EKPL T+ EA+EI A + + Sbjct: 59 KLISSDQIDAISVATPDFTHTPIILAALKADKHVLSEKPLATTVSEAEEIAAAAAISKGK 118 Query: 121 MMVDLHSRWSPLVAIPKKSIVDGDIGKPYSAYYRLNDIKWVATDLLPWAAKSSILWFLGY 180 +M+D H+R +P++A + I DG IG RL++ +V ++L WAAKSS LWFLG Sbjct: 119 LMIDFHNRVNPILAQVRDMIQDGQIGLAKHGTARLSNTTFVPFEMLSWAAKSSALWFLGS 178 Query: 181 HSVDVLRWLFDDEVERVYSVSREGVLQGEGVDTVDIYQTILEFKNGGIATMENGWITPNS 240 H VDVLR++ DEV RVYSV+R G L GVDT D + +ILEF G + TMEN WI Sbjct: 179 HLVDVLRFILADEVVRVYSVARSGTLSAGGVDTKDFHASILEFSKGTVVTMENSWILSRD 238 Query: 241 NPNVNDMKLNITGTKGMFNIDPTHSQMMERFTEMKADRPDLL-VRHFIHGKPKGFAYESI 299 NP++ D K+ G KG DPTHS + R + D + + + GF ESI Sbjct: 239 NPSLVDFKVEFVGEKGQIQADPTHSGGLRRIVDGGLKYNDYIGITPTGATRIGGFVLESI 298 Query: 300 RHFIDHLISGEPFYVSMEDAYNTTMVILAIMESAKTRTPVNV 341 F+D ++ P +D T ++ AI ES T VN+ Sbjct: 299 ARFVDSVVRDAPLLADAQDGLANTKILAAIEESVATGKAVNI 340 >ref|NP_461680.1| putative dehydrogenase [Salmonella typhimurium LT2] gb|AAL21639.1| putative dehydrogenase [Salmonella typhimurium LT2] Length = 337 Score = 174 bits (440), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 102/341 (29%), Positives = 169/341 (49%), Gaps = 4/341 (1%) Query: 1 MTVIKMGIVGAGTWGETHAFIYNDHPGAEVTAVCDMNIERAKALADKFNIPGNHVFTDHK 60 M I+ +VGAG +G+ H Y +P + A+CD + ER LA + I G +T Sbjct: 1 MNKIRAAVVGAGIYGKHHMNAYRHNPDTVLVAICDTDTERCDDLAMAYGIQG---YTRLD 57 Query: 61 EMLRSADIDAVAIVTPDFAHTRIAVDCANAGKHFIIEKPLTTSREEADEIVEAVERNNVR 120 +L+ ID V++ TPD HT + G+H + EKPL TS E + IVE ++ ++ Sbjct: 58 RLLQQEAIDIVSVATPDPYHTESILTALRHGQHVLAEKPLATSVRECELIVEMAQQRDLL 117 Query: 121 MMVDLHSRWSPLVAIPKKSIVDGDIGKPYSAYYRLNDIKWVATDLLPWAAKSSILWFLGY 180 + VD H RW P V K + + + G + ++D+ V T L WA SS +WFLG Sbjct: 118 VGVDFHKRWDPAVMRIKAELENPETGCILRGHISMDDVITVPTQWLNWAGASSPVWFLGS 177 Query: 181 HSVDVLRWLFDDEVERVYSVSREGVLQGEGVDTVDIYQTILEFKNGGIATMENGWITPNS 240 H D++R L EV VY+V ++ ++ G+DT D Q++L +G +EN W+ P Sbjct: 178 HCFDLVRHLSGQEVVSVYAVGQKRLMVECGLDTFDSVQSLLTMADGSSWGVENSWVLPEG 237 Query: 241 NPNVNDMKLNITGTKGMFNIDPTHSQMMERFTEMKADRPDLLVRHFIHGKPKGFAYESIR 300 P ND +++I + + + + +E T P+ ++ +G GF + I Sbjct: 238 FPKDNDGRIDIL-CEATYIRSTSQHRGLEITTPALTLTPNSYFINYRNGVASGFGIDPIN 296 Query: 301 HFIDHLISGEPFYVSMEDAYNTTMVILAIMESAKTRTPVNV 341 F+ + P+ V+ ED + + A+ S ++ P +V Sbjct: 297 DFVRAVRHKAPYPVTAEDGLAVSRICEAVHRSLESGKPEDV 337 >ref|NP_466322.1| similar to dehydrogenase [Listeria monocytogenes EGD-e] pir||AG1424 dehydrogenase homolog lmo2800 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD01013.1| lmo2800 [Listeria monocytogenes] Length = 333 Score = 148 bits (374), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 94/330 (28%), Positives = 165/330 (50%), Gaps = 11/330 (3%) Query: 4 IKMGIVGAGTWGETHAFIYNDHPGAEVTAVCDMNIERAKALADKFNIPGNHVFTDHKEML 63 IK+G++G G G+ HA IY P E+ A+C+ N ER +A+KF + D+ E+L Sbjct: 5 IKVGVIGVGQMGQYHAEIYQKLPQVELVAICEYNDERRAEMAEKFQCKA---YKDYHELL 61 Query: 64 RSADIDAVAIVTPDFAHTRIAVDCANAGKHFIIEKPLTTSREEADEIVEAVERNNVRMMV 123 + +I+AV+IV PD H + KH ++EKPL E+ + E + + V Sbjct: 62 ANTEIEAVSIVLPDNMHRDCVEEAVKYKKHILLEKPLAKELEDGKAMYELTKDYDKVFTV 121 Query: 124 DLHSRWSPLVAIPKKSIVDGDIGKPYSAYYRLNDIKWVATDLLPWAAKSSILWFLGYHSV 183 R+ P + K+ + +G++G Y R N T + S + + H + Sbjct: 122 GFLLRFDPRFNMIKQRLDNGELGDIIHLYCRRNS---PITGPKRYIGASDLSMHVMIHDI 178 Query: 184 DVLRWLFDDEVERVYSVSREGVLQGEGVDTVDIYQTILEFKNGGIATMENGWITPNSNPN 243 D + W D E +V + SR +L+ G++ D+ I+ ++NG IA +E W+ P ++P Sbjct: 179 DYINWYMDSEPVKVIAKSRSVLLKEYGMN--DVIYAIVTYENGAIACLEACWVLPENSPT 236 Query: 244 VNDMKLNITGTKGMFNIDPTHSQMMERF-TEMKADRPDLLVRHFIHGKPKGFAYESIRHF 302 + D KL + GTKG+ +D + RF +E PD ++ +G+ G E + F Sbjct: 237 IIDDKLELVGTKGVAYVDSCDHGV--RFVSEKGVSYPDSRHWYYTNGEVSGDLAEEVMAF 294 Query: 303 IDHLISGEPFYVSMEDAYNTTMVILAIMES 332 I+++ + ++ ++AY++ V+ AI S Sbjct: 295 INNVANNTKSIITPKEAYDSLRVVDAIERS 324 >ref|NP_472259.1| similar to dehydrogenase [Listeria innocua] pir||AE1798 dehydrogenase homolog lin2932 [imported] - Listeria innocua (strain Clip11262) emb|CAC98157.1| lin2932 [Listeria innocua] Length = 333 Score = 148 bits (373), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 94/330 (28%), Positives = 164/330 (49%), Gaps = 11/330 (3%) Query: 4 IKMGIVGAGTWGETHAFIYNDHPGAEVTAVCDMNIERAKALADKFNIPGNHVFTDHKEML 63 IK+G++G G G+ HA IY P E+ A+C+ N ER +A+KF + D+ E+L Sbjct: 5 IKVGVIGVGQMGQYHAEIYQKLPQVELVAICEFNDERRAEMAEKFQCKA---YKDYHELL 61 Query: 64 RSADIDAVAIVTPDFAHTRIAVDCANAGKHFIIEKPLTTSREEADEIVEAVERNNVRMMV 123 + +I+AV IV PD H + KH ++EKPL E+ + E + + V Sbjct: 62 ANTEIEAVIIVLPDNMHRDCVEEAVKHKKHILLEKPLAKELEDGKAMYELTKDYDKVFTV 121 Query: 124 DLHSRWSPLVAIPKKSIVDGDIGKPYSAYYRLNDIKWVATDLLPWAAKSSILWFLGYHSV 183 R+ P + K+ + +G++G Y R N T + S + + H + Sbjct: 122 GFLLRFDPRFNMIKQRLDNGELGDIIHLYCRRNS---PITGPKRYIGASDLSMHVMIHDI 178 Query: 184 DVLRWLFDDEVERVYSVSREGVLQGEGVDTVDIYQTILEFKNGGIATMENGWITPNSNPN 243 D + W D E +V + SR +L+ G++ D+ I+ ++NG IA +E W+ P ++P Sbjct: 179 DYINWYMDSEPVKVIAKSRSVLLKEYGMN--DVIYAIVTYENGAIACLEACWVLPENSPT 236 Query: 244 VNDMKLNITGTKGMFNIDPTHSQMMERF-TEMKADRPDLLVRHFIHGKPKGFAYESIRHF 302 + D KL + GTKG+ +D + RF +E PD ++ +G+ G E + F Sbjct: 237 IIDDKLELVGTKGVAYVDSCDHGV--RFVSEKGVSYPDSRHWYYTNGEVSGDLAEEVMAF 294 Query: 303 IDHLISGEPFYVSMEDAYNTTMVILAIMES 332 I+++ + ++ ++AY++ V+ AI S Sbjct: 295 INNVANNTKSIITPKEAYDSLRVVDAIERS 324 >ref|NP_613535.1| Predicted dehydrogenase [Methanopyrus kandleri AV19] gb|AAM01465.1| Predicted dehydrogenase [Methanopyrus kandleri AV19] Length = 317 Score = 135 bits (340), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 94/348 (27%), Positives = 170/348 (48%), Gaps = 47/348 (13%) Query: 4 IKMGIVGAGTWGETHAFIYNDHPGAEVTAVCDMNIERAKALADKFNIPGNHVFTDHKEML 63 ++ G++G G G HA +Y++ E+ AVCD+N R K +A K+++ + +TDH++ML Sbjct: 3 VRAGVIGVGMMGSHHARVYHELEETELVAVCDVNERRVKEVAKKYDV---NWYTDHRKML 59 Query: 64 RSADIDAVAIVTPDFAHTRIAVDCANAGKHFIIEKPLTTSREEADEIVEAVERNNVRMMV 123 + D+D V+I P H IAVD AG H ++EKP+ + E A I++A E + +++ V Sbjct: 60 QEEDLDGVSICVPTKYHADIAVDALEAGVHVLVEKPIADTIENARRIIDAAEDHGLKLAV 119 Query: 124 DLHSRWSPLVAIPKKSIVDGDIGKPYSAYYRLNDIKWVATDLLPWAAK---SSILWFLGY 180 R++P V K+ + GD+G + + A + P+ + ++ L Sbjct: 120 GHIERFNPSVMKAKEVVSRGDLG---------DIVVMSAKRVGPYPPRIRDVGVIVDLAV 170 Query: 181 HSVDVLRWLFDDEVERVYSVSREGVLQGEGVDTVDIYQTILEFKNGGIATMENGWITPNS 240 H +DV+R+L + EVE VY+ + + + + D + +L F+ +E W+TP+ Sbjct: 171 HDIDVMRYLAETEVEEVYAAAGSAITRTQE----DYAEVMLRFEGDPTGLIEVNWLTPH- 225 Query: 241 NPNVNDMKLNITGTKGMFNIDPTHSQM-------MERFTEMKADRPDLLVRHFIHGKPKG 293 + +L +TG + + I ++ ++RF K + L +R F Sbjct: 226 ----KERRLEVTGREAILEIQYIEQELRLMDREGVKRFNIRKEEPLKLELRDF------- 274 Query: 294 FAYESIRHFIDHLISGEPFYVSMEDAYNTTMVILAIMESAKTRTPVNV 341 ESI D L+ GE ++ A +A ++S K PV++ Sbjct: 275 --AESILENRDPLVDGEAGLQALRTA-------VAALKSVKEDRPVSL 313 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 526,620,907 Number of extensions: 22232761 Number of successful extensions: 56725 Number of sequences better than 1.0e-05: 1174 Number of HSP's gapped: 56131 Number of HSP's successfully gapped: 1174 Length of query: 343 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 217 Effective length of database: 415,135,104 Effective search space: 90084317568 Effective search space used: 90084317568 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)