BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0495.0004.p (310 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00183122.1| hypothetical protein Exigu147901 [Exiguobacte... 251 1e-65 ref|NP_470110.1| conserved hypothetical protein [Listeria innocu... 242 6e-63 ref|NP_816282.1| diacylglycerol kinase catalytic domain protein ... 241 2e-62 ref|NP_464301.1| conserved hypothetical protein [Listeria monocy... 241 2e-62 ref|ZP_00037893.1| COG1597: Sphingosine kinase and enzymes relat... 226 4e-58 >ref|ZP_00183122.1| hypothetical protein Exigu147901 [Exiguobacterium sp. 255-15] Length = 296 Score = 251 bits (642), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 127/295 (43%), Positives = 187/295 (63%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M++ +I+NPSSGKE + E L EV V+ T+KE DAT ++ A D Sbjct: 1 MSKVMLIINPSSGKELGKKHAELAEGTLAQRFTEVDVRFTEKELDATEFATQASLGHYDA 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 +V++GGDGT++E I G++ QAH+P LGI+PLGTVND ARAL IP PEEA+ L + Sbjct: 61 VVAMGGDGTVNEVITGIAEQAHRPTLGIVPLGTVNDLARALHIPTDPEEAISILEDAPSR 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D G+ ++ F+NV+A G +AE++ VS + K+ LG++AYF EG+K ++ +P+ + Sbjct: 121 PLDIGKYDDHYFMNVIAVGLIAEAVEEVSVEQKTSLGSVAYFIEGLKAFKDHSPYPIRLT 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 + E++ GETPL + +LTNSVGGFE A +A VDDGL+H +I+K L FF+ L +L Sbjct: 181 AEEESFDGETPLLIISLTNSVGGFESFAKDARVDDGLLHVFIVKQLGFFDALQALPKLLT 240 Query: 255 GNLKNHKDVEYFTAGEVTISSSDTVRTNVDGEEGPALPIRLSVLPRHIRVVVPDS 309 G + + VEYFT EV + S + + N DG+ LP+ + VL +H+ V P S Sbjct: 241 GKISDADQVEYFTTKEVHVDSKERLPINADGDTAGHLPVTVQVLSQHLTVFAPVS 295 >ref|NP_470110.1| conserved hypothetical protein [Listeria innocua] pir||AH1528 conserved hypothetical protein lin0768 [imported] - Listeria innocua (strain Clip11262) emb|CAC96000.1| lin0768 [Listeria innocua] Length = 309 Score = 242 bits (618), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 122/294 (41%), Positives = 182/294 (61%), Gaps = 1/294 (0%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M +A +IVNPSSGKE+ Y EE+L+ EV V+ T+K GDAT ++ A E D Sbjct: 1 MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWASEQGFDA 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 ++++GGDGTL+ETINGL+ +P G IPLGTVND AR++ IPL PE+A++ L + Sbjct: 61 VIAMGGDGTLNETINGLAIHEKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAKAI 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D GR+ + F+NV+A G +A+++ VS + K++ G++AYF EG+K N ++ Sbjct: 121 PMDIGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAYFLEGLKAFNRNELLHFKLE 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 +D E W GE L +A LT SVGG E AP+A +DDG +H ++ L + + ++ Sbjct: 181 YDEEVWEGEAALVVAGLTKSVGGMESWAPDAKIDDGYLHVVVLTKLGLLDAANMIPQLIR 240 Query: 255 GNLKNHKDVEYFTAGEVTISSS-DTVRTNVDGEEGPALPIRLSVLPRHIRVVVP 307 GNLKN V Y ++TI +S D + NVDG+ GP +P + VL H+ ++ P Sbjct: 241 GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAP 294 >ref|NP_816282.1| diacylglycerol kinase catalytic domain protein [Enterococcus faecalis V583] gb|AAO82352.1| diacylglycerol kinase catalytic domain protein [Enterococcus faecalis V583] Length = 300 Score = 241 bits (615), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 127/292 (43%), Positives = 184/292 (63%), Gaps = 2/292 (0%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M +A +IVNPSSG E+A ++ EE L+ EV VK+T+K GDA ++ A E+ D Sbjct: 1 MKKAVLIVNPSSGGEKAKEFETLAEEKLKQLFDEVVVKQTEKGGDAEQFAREAAESHFDS 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 + +GGDGT++E I+GL+ QA++PK G PLGTVND ARAL +P+ PEEA++ L + Sbjct: 61 VFVMGGDGTVNEGISGLAEQAYRPKFGFFPLGTVNDLARALNLPMDPEEAIQQLDLEKTS 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D G++N+ F+NVVA G + ES+ V + K++LG LAYF G K LA ++P + Sbjct: 121 ALDVGKINDDYFMNVVAIGTIPESINDVDVEQKTKLGKLAYFISGAKHLANAQTYPFHLS 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 D + E+ L LTNS+GGFE L PEA VDDG +H +KD ++ + +L Sbjct: 181 LDQKEQTIESSTVLVGLTNSIGGFETLLPEAQVDDGKLHLVYLKDQSLWDAVKAVPDLLK 240 Query: 255 GNLKNHKDVEYFTAGEVTIS--SSDTVRTNVDGEEGPALPIRLSVLPRHIRV 304 G ++ ++ Y T E TIS + + + TNVDG+EG ALPI L +LP+H+ V Sbjct: 241 GVDQSTDNLVYLTFKEGTISLENQEELTTNVDGDEGAALPITLKILPKHLTV 292 >ref|NP_464301.1| conserved hypothetical protein [Listeria monocytogenes EGD-e] pir||AF1171 conserved hypothetical protein lmo0774 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC98852.1| lmo0774 [Listeria monocytogenes] Length = 309 Score = 241 bits (614), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 123/294 (41%), Positives = 181/294 (61%), Gaps = 1/294 (0%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M +A +IVNPSSGKE+ Y EE+L+ EV V+ T+K GDAT ++ A E + Sbjct: 1 MGKALLIVNPSSGKEKGKTYQGKTEEVLKKRYDEVEVRLTEKAGDATEFASWAAEQGFEA 60 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 ++++GGDGTL+ETINGL+ +P G IPLGTVND AR++ IPL PE+A++ L Sbjct: 61 VIAMGGDGTLNETINGLAIHEKRPDFGFIPLGTVNDLARSVGIPLKPEKAIQALEKAIAV 120 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D GR+ + F+NV+A G +A+++ VS + K++ G++AYF EG+K N I+ Sbjct: 121 PMDVGRIGDQYFMNVLAIGMIAQAVDQVSVEQKTKFGSVAYFLEGLKAFNRNELLHFKIE 180 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 +D E W GE L +A LT SVGG E AP+A +DDG +H I+ L + + ++ Sbjct: 181 YDEEVWEGEAALVVAGLTKSVGGMESWAPDAKIDDGYLHIIILTKLGLLDAANMIPQLIR 240 Query: 255 GNLKNHKDVEYFTAGEVTISSS-DTVRTNVDGEEGPALPIRLSVLPRHIRVVVP 307 GNLKN V Y ++TI +S D + NVDG+ GP +P + VL H+ ++ P Sbjct: 241 GNLKNSDGVVYIKTKKLTIDASGDDLSINVDGDPGPGVPAEIEVLGSHLNILAP 294 >ref|ZP_00037893.1| COG1597: Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Enterococcus faecium] Length = 295 Score = 226 bits (577), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 121/292 (41%), Positives = 178/292 (60%), Gaps = 2/292 (0%) Query: 15 MTQATIIVNPSSGKEEALDYLRSVEELLQSHGYEVTVKETQKEGDATGYSIAACEAEADM 74 M + ++VNPSSG E+A ++ + L+S EV V T+K GDA ++ A Sbjct: 2 MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAGDAKNFTREAATEGYHS 61 Query: 75 IVSIGGDGTLHETINGLSGQAHQPKLGIIPLGTVNDFARALQIPLIPEEAVRTLASTRVQ 134 + +GGDGT++E I+G++ Q H+P G PLGTVND ARAL IPL PEEA+ + V+ Sbjct: 62 VFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALGIPLEPEEAINHFSIESVK 121 Query: 135 LVDAGRLNNGLFINVVAAGKLAESLTSVSSDDKSRLGALAYFKEGIKELAGNASHPLTIQ 194 +D G++N+ F+NVVA G + ES+ V ++ K++ G LAYF GIK+LA S+ ++ Sbjct: 122 PLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLAYFMSGIKQLASTQSYSFHVE 181 Query: 195 HDGETWAGETPLFLAALTNSVGGFEKLAPEAAVDDGLIHCYIIKDLHFFNTLTVSLSVLL 254 DG+ E+ L LTNSVGGFE L P A VDDG +H +KD +TL +L Sbjct: 182 VDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKLHLVYLKDSSLIDTLKTLPDLLK 241 Query: 255 GNLKNHKDVEYFTAGEVTISSSD--TVRTNVDGEEGPALPIRLSVLPRHIRV 304 G ++ K++ Y T ++ +S +D + TNVDG+EG LP+ + VLP H++V Sbjct: 242 GVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEGDKLPVTIKVLPSHLQV 293 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 443,447,827 Number of extensions: 17720170 Number of successful extensions: 56085 Number of sequences better than 1.0e-05: 225 Number of HSP's gapped: 55862 Number of HSP's successfully gapped: 225 Length of query: 310 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 185 Effective length of database: 417,850,203 Effective search space: 77302287555 Effective search space used: 77302287555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 126 (53.1 bits)