BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0493.0022.p (570 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_388576.1| similar to two-component sensor histidine kinas... 166 1e-39 ref|ZP_00100011.1| COG2972: Predicted signal transduction protei... 162 2e-38 ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongen... 159 1e-37 ref|NP_563004.1| two-component sensor histidine kinase [Clostrid... 156 1e-36 ref|NP_244314.1| two-component sensor histidine kinase [Bacillus... 155 2e-36 >ref|NP_388576.1| similar to two-component sensor histidine kinase [YesN] [Bacillus subtilis] pir||D69796 two-component sensor histidine kinase homolog yesM - Bacillus subtilis emb|CAB12514.1| yesM [Bacillus subtilis subsp. subtilis str. 168] Length = 577 Score = 166 bits (421), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 104/310 (33%), Positives = 173/310 (55%), Gaps = 18/310 (5%) Query: 258 IPKKQVLSKLSESMRNYIRIIALIAF-VSLAAGVLLSLRFTRVIRDLLRSIHKVK----K 312 IP Q+ +K+S M+ I L+ F V L G ++ T I L+ ++ V+ + Sbjct: 276 IPFDQMFAKIS-FMKTVIGTCFLLFFCVVLLFGRKIANSITEPIEQLVTAMKSVQHSGIE 334 Query: 313 GDYNVRMPAYKNVELNQLSTTFNRMTEEINYLIKEVYEKHLLIKESEIKFLQAQINPHFL 372 ++ +P + E L+ F M + IN L++E EK L+IKE+E+K LQAQINPHFL Sbjct: 335 AGVSLSLPEHTQDEAGMLNRHFTVMMKRINELMEENVEKQLIIKETELKALQAQINPHFL 394 Query: 373 FNTLITIGYKAKLSKDETVYKMVTSLTELLQAGIYSNSLAKVPIRQELDVIQFYLYLQKE 432 +NTL +I + AK ++ + + KMV SL LL+ I+ V I++E D+++ Y+ +Q+ Sbjct: 395 YNTLESINWLAKANQQKQISKMVESLGFLLRNSIHMKK-DIVTIQEEADIVRHYMTIQRF 453 Query: 433 RFEDKIEYTIHIEDETILDLLLPKLSVEPLVENAVVHGVEKKLGKGIIHIRIYRRNESVY 492 RFE+++++T+ I+DE + L+PKL+++PL ENA+ + +E I I+ + V Sbjct: 454 RFEERLKFTLDIDDE-VKHCLIPKLTLQPLAENAIQYALEPFTRPCAIRIQAKKAKGCVC 512 Query: 493 FEITDNGNGFEVVPRNWSNFESITIRKQGHNNIGLINTQKRVKLIYGEPYGIEVESEFGA 552 + DNG G + + G IGL N ++R+ L +GEPYG+ + SE Sbjct: 513 ITVEDNGPG----------MDGRILESTGGRGIGLWNIRERISLTFGEPYGLRIHSEHEK 562 Query: 553 GAKVTLHIPA 562 G ++ + IP Sbjct: 563 GTRIVITIPC 572 >ref|ZP_00100011.1| COG2972: Predicted signal transduction protein with a C-terminal ATPase domain [Desulfitobacterium hafniense] Length = 577 Score = 162 bits (411), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 95/272 (34%), Positives = 151/272 (55%), Gaps = 17/272 (6%) Query: 297 TRVIRDLLRSIHKVKKGDYNVRMPAYKNVELNQLSTTFNRMTEEINYLIKEVYEKHLLIK 356 R IR L +++ +V+KG + + E+ LST F M + I L++E+ K ++ Sbjct: 316 ARPIRQLEKAMEEVEKGSLATEVGVVGSAEIASLSTHFLSMLDRIRELMQEIRNKEQFLR 375 Query: 357 ESEIKFLQAQINPHFLFNTLITIGYKAKLSKDETVYKMVTSLTELLQAGIYSNSLAKVPI 416 SE+K L +QINPHFL+NTL TI + A+ E V + ++ + + S + Sbjct: 376 ASELKTLYSQINPHFLYNTLDTIVWLAEFGNTERVITVSKAMARFFRLSLRGGS-ETTTV 434 Query: 417 RQELDVIQFYLYLQKERFEDKIEYTIHIEDETILDLLLPKLSVEPLVENAVVHGVEKKLG 476 R ELD ++ YL++QKER++DK+ Y I ED ++D+ +PK+ ++PLVENA+ HG+ K G Sbjct: 435 RDELDHVRQYLFIQKERYQDKLSYEIQAED-GLMDITIPKILLQPLVENAIYHGLRKMPG 493 Query: 477 KGIIHIRIYR-RNESVYFEITDNGNGFEVVPRNWSNFESITIRKQGHNNIGLINTQKRVK 535 G+I I + E ++ + DNG GFE + +R G +GL N +R++ Sbjct: 494 GGMIRITARKGEKEDLFLVVEDNGGGFEDAEKG-----KDPLRLGG---VGLKNVAERIR 545 Query: 536 LIYGEPYGIEVESEFGAGA------KVTLHIP 561 L YGE YG+ + + G GA K TL +P Sbjct: 546 LYYGEDYGLSIANRPGQGATIILRLKTTLPVP 577 >ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongensis] gb|AAM25485.1| predicted ATPase [Thermoanaerobacter tengcongensis] Length = 593 Score = 159 bits (403), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 109/344 (31%), Positives = 188/344 (54%), Gaps = 12/344 (3%) Query: 224 LKDNAEVREVKLNHESYLTVSRSIGTTGLTFIAGIPKKQVLSKLSES-MRNYIRIIALIA 282 LKD V + +N L S I T+ ++ + K ++ + S + Y+RI+ Sbjct: 252 LKDKQNVV-ININSSKLLVTSSPIPTSDWRLVSVVSLKNAINSIRISFIEFYLRILVFCL 310 Query: 283 FVSLAAGVLLSLRFTRVIRDLLRSIHKVKKGDY--NVRMPAYKNVELNQLSTTFNRMTEE 340 + + S T + L S+ KV++G+ V + E+ + +NRM E Sbjct: 311 LFAFLVSMYFSRALTHPLERLANSMKKVRQGNLEEKVEFEYHSKDEIAIVIKEYNRMVER 370 Query: 341 INYLIKEVYEKHLLIKESEIKFLQAQINPHFLFNTLITIGYKAKLSKDETVYKMVTSLTE 400 IN LI + + + +E+E+K L+AQINPHFL+NTL TI +KA+ +KD + +M+ SL+ Sbjct: 371 INDLINRILKLEINKREAELKALEAQINPHFLYNTLDTIFWKAEKAKDRELSEMIYSLSR 430 Query: 401 LLQAGIYSNSLAKVPIRQELDVIQFYLYLQKERFEDKIEYTIHIEDETILDLLLPKLSVE 460 L + + + + +R E ++I+ YL+LQ +R+++K +Y++ I DE ILD +PKL ++ Sbjct: 431 LFRLTL-NRGEEFIQVRGEKELIERYLFLQSKRYKNKFKYSVKISDE-ILDYYIPKLILQ 488 Query: 461 PLVENAVVHGVEKKLGKGIIHIRIYRRNESVYFEITDNGNGF--EVVPRNWSNFESITI- 517 P VENA+VHG+E + + + + E++ F I DNG G E + + ES I Sbjct: 489 PFVENAIVHGLENNSEESFVEVIGEKEGENLKFIIKDNGPGMSEETLEKLKRFIESENIE 548 Query: 518 RKQGHNNIGLINTQKRVKLIYGEPYGIEVESEFGAGAKVTLHIP 561 K G+ + N +R++L YG+ Y +++ES G G ++L +P Sbjct: 549 EKVGY---AIRNVNERLRLYYGDKYELKIESRQGEGTTISLILP 589 >ref|NP_563004.1| two-component sensor histidine kinase [Clostridium perfringens] dbj|BAB81794.1| two-component sensor histidine kinase [Clostridium perfringens str. 13] Length = 578 Score = 156 bits (394), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 101/290 (34%), Positives = 167/290 (57%), Gaps = 12/290 (4%) Query: 283 FVSLAAGVLLSLRFTRV---IRDLLRSIHKVKKGDYNVRMPAYKNV-ELNQLSTTFNRMT 338 F+ + A +L ++F + + L+ ++ KV+KGD +V +P K+ E+ ++ FN+M Sbjct: 295 FLFIIAECVLYIKFKNLSSRMDSLVLAMEKVEKGDIDVHIPLSKDRDEITFIADNFNKMC 354 Query: 339 EEINYLIKEVYEKHLLIKESEIKFLQAQINPHFLFNTLITIGYKAKLSKDETVYKMVTSL 398 E++N I++ Y L +E+EI LQ QINPHFL+NTL +I KA + D+ V KM+ SL Sbjct: 355 EKLNDHIRKSYLAELRQREAEIVALQNQINPHFLYNTLESIRMKAICNGDKEVGKMLYSL 414 Query: 399 TELLQAGIYSNSLAKVPIRQELDVIQFYLYLQKERFEDKIEYTIHIEDETILDLLLPKLS 458 L ++ + N + V I QE+ YL L K R+++K ++ I E E + D + K + Sbjct: 415 AFLFRSQVKGNLI--VSIDQEIKYCNKYLELFKFRYDEKFKFKIECE-EDLYDKGIVKFT 471 Query: 459 VEPLVENAVVHGVEKKLGKGIIHIRIYRRNESVYFEITDNGNGFEVVPRNWSNFESITIR 518 ++PL+EN VHG+ + + I IY+ E + E+ DNG G +P+ + + IR Sbjct: 472 LQPLIENYFVHGIRLERDDNELKIYIYKHLEDIVVEVIDNGRG---IPKEKLDDINRRIR 528 Query: 519 KQGHN--NIGLINTQKRVKLIYGEPYGIEVESEFGAGAKVTLHIPADQGE 566 + H+ +IG++N +R+K+ YGEPYG+ V SE G + L P + E Sbjct: 529 ECDHSGKSIGMLNVHERIKIKYGEPYGLTVTSEENKGTNMILKFPLREVE 578 >ref|NP_244314.1| two-component sensor histidine kinase [Bacillus halodurans] pir||G84080 two-component sensor histidine kinase BH3447 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07166.1| two-component sensor histidine kinase [Bacillus halodurans] Length = 602 Score = 155 bits (393), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 141/577 (24%), Positives = 270/577 (46%), Gaps = 39/577 (6%) Query: 10 WNISQILTKNANDNLSLLIRQTNDNIEKSFQIIDNTSLHFLANKTIRTW---SIEDVSLG 66 W + +I+ + N+N LI + N IE + + N + + I+ + ++E + Sbjct: 37 WAVDRIIEQQVNENTLQLIDKVNSTIEAHIENVQNMTYFISLHSNIQQFLDGTLEHLGPD 96 Query: 67 DDY-YKKFLNKSEMEEDLKYSLMFNNAWNISLLSTAYVFFDKDNYISVLKSQPNIEQINK 125 ++Y KFL ++ ++ I +++ + + Y +S N+ Q + Sbjct: 97 EEYEISKFLQN--------FTTLYPEIAGILVVNQQGDYLSNELYT---RSSFNLNQESW 145 Query: 126 NNLAV----FHSVNGRVRGKEIITPSY--EDRTLYFTRIVSNINLPQQRLVLIFGTDEAD 179 AV + G+ +G+ I + + ED + R + + +++ V++ Sbjct: 146 YRQAVENKGIAKLIGKPQGRRITSHVHYREDEVVTVVRAIIDSTTGEEKGVILIDLKLRV 205 Query: 180 LADQYSGLLAFPGAMAYIIDNRG-AIYSSADKQELGSIVSPAVF--ALKDNAEVREVKLN 236 + + + ++D +G IYS A SI+ F N + ++ Sbjct: 206 IEEAVRDVRLGKSGYLMVVDQKGDPIYSPAP-----SIMYEQSFDHRFSQNYGIFTEEVE 260 Query: 237 HESYLTVSRSIGTTGLTFIAGIPKKQVLSKLSESMRNYIRIIALIAFVSLAAGVLLSLRF 296 E Y + + TG T I Q + ++ + + + + + FV + A LS Sbjct: 261 GERYQLMYQQSAFTGWTLIGVFSTDQSVEEVQQILFYVVTFVFFVCFVGITASYFLSYTI 320 Query: 297 TRVIRDLLRSIHKVKKGDYNVRMPAYKNVELNQLSTTFNRMTEEINYLIKEVYEKHLLIK 356 +R I L+ ++ K + GD R + E+ +L +FN M E+I LI + + Sbjct: 321 SRPINQLMLAMQKAESGDLTSRYEGEEQDEVGRLGRSFNTMIEQIGKLISLTERQERKKR 380 Query: 357 ESEIKFLQAQINPHFLFNTLITIGYKAKLSKDETVYKMVTSLTELLQAGIYSNSLAKVPI 416 E+E+ LQA I PHFL+NTL TI + A+ V +V +L++L + G+ S +P+ Sbjct: 381 EAELHSLQANIKPHFLYNTLDTIQWMARKKNASDVADLVGALSKLFRIGL-SKGGTIIPL 439 Query: 417 RQELDVIQFYLYLQKERFEDKIEYTIHIEDETILDLLLPKLSVEPLVENAVVHGVEKKLG 476 E++ I+ YL +Q R+++K+ Y++ + + L + K+ ++P+VENA+ HG++++ G Sbjct: 440 VDEIEHIKNYLQIQSVRYKNKLNYSL-VMPSNMERLSILKIVLQPIVENAIYHGIKERRG 498 Query: 477 KGIIHIRIYRRNESVYFEITDNGNGF--EVVPRNWSNFESITIR--KQGHNNI----GLI 528 G+I I R N+ + I DNG G E + + +N + K+G + GLI Sbjct: 499 PGLITIEAERENDMLIIRIRDNGKGIKPETLQQLRTNVNLFLEKGDKEGGQRLTKGYGLI 558 Query: 529 NTQKRVKLIYGEPYGIEVESEFGAGAKVTLHIPADQG 565 N R++L +GEPYG++++S F G VT+ P +G Sbjct: 559 NVHARLRLTFGEPYGVKIDSVFNEGTVVTIFHPILEG 595 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 817,664,801 Number of extensions: 35297951 Number of successful extensions: 110360 Number of sequences better than 1.0e-05: 188 Number of HSP's gapped: 110111 Number of HSP's successfully gapped: 188 Length of query: 570 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 439 Effective length of database: 401,559,609 Effective search space: 176284668351 Effective search space used: 176284668351 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)