BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0053.p (366 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAB61395.1| germination protein 213 6e-54 ref|NP_242802.1| spore germination protein KB [Bacillus halodura... 193 5e-48 ref|NP_388254.1| gerKB [Bacillus subtilis] >gi|1708044|sp|P49940... 189 6e-47 ref|NP_149186.1| Spore germination protein, GRKB [Clostridium ac... 130 5e-29 ref|NP_348154.1| Spore germination protein GerKB [Clostridium ac... 113 7e-24 >gb|AAB61395.1| germination protein Length = 329 Score = 213 bits (542), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 123/329 (37%), Positives = 178/329 (54%), Gaps = 1/329 (0%) Query: 1 MGTVKIGMIQFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRK 60 M KI Q F + +LFELG+AL+V L M A +DAW F Y LY Sbjct: 1 MEKAKISATQLFILMVLFELGSALLVPLAMGAKQDAWLAILIGMLCSFFFFLIYHKLYSY 60 Query: 61 YPGMSFTAYTRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXX 120 YP + T Y +K+LGK +GT +A +YI+ ++ A+R LRD ML +TPLF Sbjct: 61 YPDLLPTEYMQKILGKVLGTLLAFIYIVYFLYDASRVLRDFGEMLLTFAYPDTPLFIANA 120 Query: 121 XXXXXXAYVLHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEP 180 Y+ KG E +++ SG I+ L PVL++G P Sbjct: 121 LLMLVIIYITRKGIEAIARSGELLFIFMYFLAIAGFILIISSGLIDFKNLQPVLKEGILP 180 Query: 181 VIRSVLHQNYMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLG 240 V + L + FPF E I FTML+P+LSN KK + A +S + L TM +NISVLG Sbjct: 181 VAKVALTETLYFPFTEAIVFTMLLPYLSNPKKANVTMLCATGLSGINLIITMIINISVLG 240 Query: 241 ADIVERSPLPLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGI 300 D+ RS PL+ T+ I ++DF++R+D+F + ++IG++FK+S+ F A++I + LF I Sbjct: 241 VDLTTRSQFPLLSTVQTIQVADFLERLDVFFMFAMVIGIYFKVSILFYASIISTATLFKI 300 Query: 301 PY-RRMLYPCTLIILFTSMLDARSFTEHL 328 R+ YP +I+LF S+ A +F EHL Sbjct: 301 KSPSRLAYPLGIIVLFLSITIASNFQEHL 329 >ref|NP_242802.1| spore germination protein KB [Bacillus halodurans] pir||H83891 spore germination protein KB gerKB [imported] - Bacillus halodurans (strain C-125) dbj|BAB05655.1| spore germination protein KB [Bacillus halodurans] Length = 373 Score = 193 bits (491), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 117/351 (33%), Positives = 188/351 (53%), Gaps = 4/351 (1%) Query: 10 QFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRKYPGMSFTAY 69 QFFS+ +LFELG+A+VV +G+ AG+DAW ++ YL+ +P ++ AY Sbjct: 11 QFFSMIVLFELGSAIVVGVGLDAGQDAWMTVLIAMLAGLVLYCVLYYLHSHFPSDTWIAY 70 Query: 70 TRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXXXXXXXXAYV 129 +K+L IG + +Y + +I +A R LRD +L + + +TPL YV Sbjct: 71 LQKVLTVPIGWFIGFLYCLYFIYIATRVLRDFGDLLTTSMLTDTPLMVINLLMVFVIVYV 130 Query: 130 LHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEPVIRSVLHQN 189 L G ++ + N+ L+PVLE+G PV+R Sbjct: 131 LFHGINVLARTGEFFLIILFFIGFFSVTLIFSTDLYNIKNLMPVLENGWMPVLREAFPVT 190 Query: 190 YMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLGADIVERSPL 249 + FPFGE+I FTML FL+ + G + A+ +S L+L+ T L I VLG DI R+ Sbjct: 191 FTFPFGEIIVFTMLFHFLNKKEMGLRIGLLAIGMSGLLLALTTILYIIVLGVDITNRATF 250 Query: 250 PLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGIPYRRMLYPC 309 PL+ + KI +DFI+R+D V VL++ +FFK+++FF A + G S+LF + + L P Sbjct: 251 PLLTMVGKIQAADFIERLDALAVTVLVVAMFFKVAIFFYAGVAGFSELFKVDKFQKLLPL 310 Query: 310 -TLIILFTSMLDARSFTEHLDEGGRLL--YTVYPFLMIAIPAVLILVSVVK 357 I++F S+ A +F+EHL EG ++ Y PF + IP +++ + +++ Sbjct: 311 FGAILVFNSVHIAANFSEHLKEGLEVVPYYLHLPF-QVGIPLIVLAIVLIR 360 >ref|NP_388254.1| gerKB [Bacillus subtilis] sp|P49940|GRKB_BACSU Spore germination protein KB pir||I39860 germination response protein gerKB - Bacillus subtilis dbj|BAA11256.1| GerKB [Bacillus subtilis] dbj|BAA09004.1| spore germination protein GerKB [Bacillus subtilis] emb|CAB12180.1| gerKB [Bacillus subtilis subsp. subtilis str. 168] Length = 373 Score = 189 bits (481), Expect = 6e-47, Method: Compositional matrix adjust. Identities = 113/332 (34%), Positives = 175/332 (52%), Gaps = 1/332 (0%) Query: 1 MGTVKIGMIQFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRK 60 M +I + Q F + ++FELG++L++ G AGRDAW +F Y +Y+ Sbjct: 1 MEKARISIRQLFVMIIIFELGSSLLITPGSMAGRDAWIAVLLGCAIGLFLFYLYQGIYQC 60 Query: 61 YPGMSFTAYTRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXX 120 YP S Y +LG + + +YI+ + +AAR LRD ML H+TP+ Sbjct: 61 YPNSSPKEYMDDMLGTKLSWLFSFLYILYFAYIAARVLRDFGEMLLTFAYHDTPIIIVNA 120 Query: 121 XXXXXXAYVLHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEP 180 Y + KG E +++ SG+I+ + L PVL +G P Sbjct: 121 LLMVVSIYAVRKGIEVLARAAELLFGAMYLLGAIGLVLIIVSGTIDPHNLKPVLANGISP 180 Query: 181 VIRSVLHQNYMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLG 240 V+ SV Q PFGE++ F M+ P L++ K + AM +S L+++ T+A+NISVL Sbjct: 181 VLHSVFTQTMYVPFGEVVLFVMIFPNLNDRKDVKKMGMIAMAISGLIVALTVAINISVLD 240 Query: 241 ADIVERSPLPLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGI 300 D+ RS PL+ TI I + +F+ R+D+F ++ LIIG FFK+S++ A ++G S LF Sbjct: 241 VDLTLRSQFPLLSTIQTIKVEEFLDRLDVFFMLALIIGGFFKVSLYLYATVVGTSTLFKE 300 Query: 301 PY-RRMLYPCTLIILFTSMLDARSFTEHLDEG 331 ++ YP L IL S+ A +F+EHL+EG Sbjct: 301 KNPSQLAYPMGLGILILSITIATNFSEHLNEG 332 >ref|NP_149186.1| Spore germination protein, GRKB [Clostridium acetobutylicum] gb|AAK76768.1| Spore germination protein, GRKB [Clostridium acetobutylicum] Length = 361 Score = 130 bits (327), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 83/325 (25%), Positives = 158/325 (48%), Gaps = 2/325 (0%) Query: 4 VKIGMIQFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRKYPG 63 +++ Q F++ +FE+G+ + LG+ A ++AW + Y L + +P Sbjct: 2 IRLSKHQLFTLIFVFEVGSTPIFALGIEAEQNAWIVVLLALLIGLFILWSYLELQKAFPT 61 Query: 64 MSFTAYTRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXXXXX 123 +LGK +G P+ ++Y + +I R+LR+ ++AM + +TPL+ Sbjct: 62 KGLVEIFTNILGKPLGLPLCILYAMGFIWGCGRNLREFIELIAMTVIPHTPLWIILFFFI 121 Query: 124 XXXAYVLHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEPVIR 183 YV KGFE ++ SG ++ L P+L DG +P+++ Sbjct: 122 GLSLYVSVKGFEVFARTTEFIFPTIIFFLIILFALISISGEVHFKNLTPILGDGLKPILK 181 Query: 184 SVLHQNYMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLGADI 243 S L FPFGE+ F M +++++ A+++S ++L FT +NI LGA Sbjct: 182 S-LPGVVWFPFGEIYVFLMYWHYVNDINTIKKASFKAVILSGILLCFTTMINICTLGAKY 240 Query: 244 VERSPLPLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGIPYR 303 + +PL+ T+ I+I D I +D+ V+++ +G FFK++++ I I+ +F I Sbjct: 241 TSIATIPLVETLRLINIGDIITHLDVIGVIIIFLGGFFKMTLYLNGIAIIINSVFSIKKS 300 Query: 304 RMLYPCT-LIILFTSMLDARSFTEH 327 +++ T + +++ S+ SF H Sbjct: 301 QLVLILTGIFMMYFSIYFEPSFAYH 325 >ref|NP_348154.1| Spore germination protein GerKB [Clostridium acetobutylicum] pir||C97088 spore germination protein GerKB [imported] - Clostridium acetobutylicum gb|AAK79494.1| Spore germination protein GerKB [Clostridium acetobutylicum] Length = 365 Score = 113 bits (283), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 84/319 (26%), Positives = 156/319 (48%), Gaps = 2/319 (0%) Query: 10 QFFSITLLFELGTALVVNLGMAAGRDAWXXXXXXXXXXXXVFAGYAYLYRKYPGMSFTAY 69 Q F++T +F++G+ + LG+ A +DAW + L+ +P + Sbjct: 8 QLFTLTFIFQIGSTPLFALGIEAEQDAWIVILLALLIGLLMAWISTELFYIFPDKNLIEI 67 Query: 70 TRKLLGKYIGTPVALVYIILYINLAARDLRDGSTMLAMATMHNTPLFXXXXXXXXXXAYV 129 +LGK +G P +VY +++I R+LR+ S ++ + ++ + L+ Y Sbjct: 68 INIILGKKLGLPFCIVYAMVFIWACGRNLREFSELILLISLPHIHLWIIILSYILLALYT 127 Query: 130 LHKGFEXXXXXXXXXXXXXXXXXXXXXXMLMFSGSINLNRLLPVLEDGAEPVIRSVLHQN 189 L KGFE +++F S ++ L+P+L +G +PV++S L Sbjct: 128 LFKGFETLCRLSELIVPPTIIFLISMFIIIIFFSSAHIRNLMPILGNGIKPVLKS-LPGV 186 Query: 190 YMFPFGEMIAFTMLMPFLSNVKKGPWVIAGAMLVSALMLSFTMALNISVLGADIVERSPL 249 FPFGE+ + +L++ K A++ S L+L + A+ IS LGA S + Sbjct: 187 IWFPFGEVFVLLLYWHYLNDKKSVRKTTFKALISSGLLLCISTAVTISELGAKYTSVSTI 246 Query: 250 PLMPTISKISISDFIQRVDIFVVMVLIIGVFFKISVFFAAALIGISDLFGIPYRRM-LYP 308 PL+ T+ I+I + + R+DI + LI+G FFKI ++ + + I+ +F I + ++ L Sbjct: 247 PLVETLRLINIVEVLNRLDILGIPFLILGGFFKICIYLNSIALTINSVFKINHFKLTLML 306 Query: 309 CTLIILFTSMLDARSFTEH 327 C + +LF S+ ++ H Sbjct: 307 CGVFMLFFSIYFEPNYAYH 325 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.331 0.144 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 417,046,530 Number of extensions: 14464524 Number of successful extensions: 48016 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 47988 Number of HSP's successfully gapped: 21 Length of query: 366 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 239 Effective length of database: 412,420,005 Effective search space: 98568381195 Effective search space used: 98568381195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 126 (53.1 bits)