BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0048.p (185 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243378.1| guanylate kinase [Bacillus halodurans] >gi|1343... 268 7e-71 ref|ZP_00099422.1| COG0194: Guanylate kinase [Desulfitobacterium... 241 7e-63 ref|NP_389450.1| similar to guanylate kinase [Bacillus subtilis]... 231 9e-60 ref|ZP_00036344.1| COG0194: Guanylate kinase [Enterococcus faecium] 230 1e-59 sp|O34328|KGUA_BACSU Guanylate kinase (GMP kinase) >gi|32468748|... 230 1e-59 >ref|NP_243378.1| guanylate kinase [Bacillus halodurans] sp|Q9K9Y2|KGUA_BACHD Guanylate kinase (GMP kinase) pir||H83963 guanylate kinase gmk [imported] - Bacillus halodurans (strain C-125) dbj|BAB06231.1| guanylate kinase [Bacillus halodurans] Length = 204 Score = 268 bits (684), Expect = 7e-71, Method: Compositional matrix adjust. Identities = 123/183 (67%), Positives = 156/183 (85%) Query: 1 LLIILSGPSGVGKGTVCTALRPKMPELVYSVSATTRQPREGEENGVNYFFKTREQFAAMI 60 LLI+LSGP+GVGKGTVC+ALR + YSVSATTR PR+GE +GVNYFFK+REQF AMI Sbjct: 7 LLIVLSGPAGVGKGTVCSALRKHDTNIQYSVSATTRAPRKGEVDGVNYFFKSREQFEAMI 66 Query: 61 EADQLLEYAEYVGNYYGTPRDFVERTLDSGRDIILEIEVQGALKVKEKFPEGIFVFLLPP 120 E ++LLE+AEYVGNYYGTP +V T+DSG+DIILEIEVQGALKV+E+FPEG+F+FL+PP Sbjct: 67 EKEELLEWAEYVGNYYGTPIQYVRETIDSGKDIILEIEVQGALKVRERFPEGVFIFLMPP 126 Query: 121 SMDELKDRIRGRGTEHPDVISHRMSVAEDEISLIRHYDYAVVNDEIDLACKRIESIIIAE 180 S+ EL+ RI GRGTE +VI+ RM+VA++EI +++ YDY V NDE++LA RI++I+ AE Sbjct: 127 SLAELRSRIVGRGTETEEVINKRMNVAKEEIEMMKKYDYVVENDEVELAVDRIKAIVTAE 186 Query: 181 HCK 183 HCK Sbjct: 187 HCK 189 >ref|ZP_00099422.1| COG0194: Guanylate kinase [Desulfitobacterium hafniense] Length = 239 Score = 241 bits (614), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 107/185 (57%), Positives = 147/185 (79%) Query: 1 LLIILSGPSGVGKGTVCTALRPKMPELVYSVSATTRQPREGEENGVNYFFKTREQFAAMI 60 LLI+LSGPSG GKGT+C L ++P++ YSVSATTRQPR GE +G++Y+F++RE+F MI Sbjct: 46 LLIVLSGPSGAGKGTLCQELLRQLPQVKYSVSATTRQPRPGEVDGLHYYFRSREEFQTMI 105 Query: 61 EADQLLEYAEYVGNYYGTPRDFVERTLDSGRDIILEIEVQGALKVKEKFPEGIFVFLLPP 120 E DQLLE+AE+ GNYYGTP+ VE+ + +G D+ILEIE+QGAL+VK++FP+G+F+F++PP Sbjct: 106 EQDQLLEWAEFCGNYYGTPQFAVEQAIQAGNDVILEIEIQGALQVKQRFPQGVFIFVVPP 165 Query: 121 SMDELKDRIRGRGTEHPDVISHRMSVAEDEISLIRHYDYAVVNDEIDLACKRIESIIIAE 180 SMDEL RI RGTE +VI R+ A E+ + YDY VVNDEI LA +++SI++AE Sbjct: 166 SMDELSQRIHKRGTESEEVIQKRLQTAARELEYVSEYDYVVVNDEIPLAVDKLKSILLAE 225 Query: 181 HCKVR 185 C+V+ Sbjct: 226 KCRVK 230 >ref|NP_389450.1| similar to guanylate kinase [Bacillus subtilis] pir||B69878 guanylate kinase homolog yloD - Bacillus subtilis emb|CAA74271.1| putative Gmk protein [Bacillus subtilis] Length = 244 Score = 231 bits (588), Expect = 9e-60, Method: Compositional matrix adjust. Identities = 109/184 (59%), Positives = 142/184 (77%), Gaps = 1/184 (0%) Query: 1 LLIILSGPSGVGKGTVCTALRPKM-PELVYSVSATTRQPREGEENGVNYFFKTREQFAAM 59 LLI+LSGPSGVGKGTV A+ + + YS+S TTR PREGE NGV+YFFKTR++F M Sbjct: 46 LLIVLSGPSGVGKGTVRQAIFSQEDTKFEYSISVTTRSPREGEVNGVDYFFKTRDEFEQM 105 Query: 60 IEADQLLEYAEYVGNYYGTPRDFVERTLDSGRDIILEIEVQGALKVKEKFPEGIFVFLLP 119 I ++LLE+AEYVGNYYGTP D+VE+TL G+D+ LEIEVQGAL+V+ FPEG+F+FL P Sbjct: 106 IADNKLLEWAEYVGNYYGTPVDYVEQTLQDGKDVFLEIEVQGALQVRNAFPEGLFIFLAP 165 Query: 120 PSMDELKDRIRGRGTEHPDVISHRMSVAEDEISLIRHYDYAVVNDEIDLACKRIESIIIA 179 PS+ ELK+RI RGTE +I +RM A+ EI ++ YDY V ND ++ AC +I++I++A Sbjct: 166 PSLSELKNRIVTRGTETDALIENRMKAAKAEIEMMDAYDYVVENDNVETACDKIKAIVLA 225 Query: 180 EHCK 183 EH K Sbjct: 226 EHLK 229 >ref|ZP_00036344.1| COG0194: Guanylate kinase [Enterococcus faecium] Length = 204 Score = 230 bits (587), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 111/185 (60%), Positives = 146/185 (78%), Gaps = 1/185 (0%) Query: 1 LLIILSGPSGVGKGTVCTAL-RPKMPELVYSVSATTRQPREGEENGVNYFFKTREQFAAM 59 LLI+LSGPSGVGKGTV A+ + + YS+S TTR+ REGE GV+YFF+++E+F +M Sbjct: 6 LLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKMREGEVEGVDYFFRSKEEFESM 65 Query: 60 IEADQLLEYAEYVGNYYGTPRDFVERTLDSGRDIILEIEVQGALKVKEKFPEGIFVFLLP 119 IEA ++LEYAEYVGNYYGTP +V +TLD G+D+ LEIEVQGA +VKEK P+G+F+FL P Sbjct: 66 IEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLEIEVQGAKQVKEKVPDGVFIFLTP 125 Query: 120 PSMDELKDRIRGRGTEHPDVISHRMSVAEDEISLIRHYDYAVVNDEIDLACKRIESIIIA 179 P + ELK RI GRGT+ +VI RM VA++EI ++ YDYAVVNDE+ LA +RI++II + Sbjct: 126 PDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMMALYDYAVVNDEVPLAVERIKNIIAS 185 Query: 180 EHCKV 184 EH +V Sbjct: 186 EHFRV 190 >sp|O34328|KGUA_BACSU Guanylate kinase (GMP kinase) emb|CAB13441.2| guanylate kinase [Bacillus subtilis subsp. subtilis str. 168] Length = 204 Score = 230 bits (586), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 109/184 (59%), Positives = 142/184 (77%), Gaps = 1/184 (0%) Query: 1 LLIILSGPSGVGKGTVCTALRPKM-PELVYSVSATTRQPREGEENGVNYFFKTREQFAAM 59 LLI+LSGPSGVGKGTV A+ + + YS+S TTR PREGE NGV+YFFKTR++F M Sbjct: 6 LLIVLSGPSGVGKGTVRQAIFSQEDTKFEYSISVTTRSPREGEVNGVDYFFKTRDEFEQM 65 Query: 60 IEADQLLEYAEYVGNYYGTPRDFVERTLDSGRDIILEIEVQGALKVKEKFPEGIFVFLLP 119 I ++LLE+AEYVGNYYGTP D+VE+TL G+D+ LEIEVQGAL+V+ FPEG+F+FL P Sbjct: 66 IADNKLLEWAEYVGNYYGTPVDYVEQTLQDGKDVFLEIEVQGALQVRNAFPEGLFIFLAP 125 Query: 120 PSMDELKDRIRGRGTEHPDVISHRMSVAEDEISLIRHYDYAVVNDEIDLACKRIESIIIA 179 PS+ ELK+RI RGTE +I +RM A+ EI ++ YDY V ND ++ AC +I++I++A Sbjct: 126 PSLSELKNRIVTRGTETDALIENRMKAAKAEIEMMDAYDYVVENDNVETACDKIKAIVLA 185 Query: 180 EHCK 183 EH K Sbjct: 186 EHLK 189 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.139 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 278,554,594 Number of extensions: 11308589 Number of successful extensions: 42218 Number of sequences better than 1.0e-05: 740 Number of HSP's gapped: 41652 Number of HSP's successfully gapped: 740 Length of query: 185 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 67 Effective length of database: 436,855,896 Effective search space: 29269345032 Effective search space used: 29269345032 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)