BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0028.p (327 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAE11242.1| hypothetical protein [Bacillus amyloliquefaciens] 581 e-164 ref|NP_831586.1| Carboxylic ester hydrolase [Bacillus cereus ATC... 77 4e-13 gb|EAE24421.1| unknown [environmental sequence] 65 2e-09 gb|EAC76171.1| unknown [environmental sequence] 64 4e-09 gb|EAK00707.1| unknown [environmental sequence] 62 2e-08 >emb|CAE11242.1| hypothetical protein [Bacillus amyloliquefaciens] Length = 402 Score = 581 bits (1497), Expect = e-164, Method: Compositional matrix adjust. Identities = 280/327 (85%), Positives = 301/327 (92%), Gaps = 3/327 (0%) Query: 1 MFPEVYDMLNEQPLISAYLEGKGFFSIDIKTQCYDDLALSGKEKRYPVLFYVCGGGGSPE 60 MFPEV +ML+EQPL+SA K +SIDIKTQCYDDLALSGKEKRYPVLFYVCGGGGSPE Sbjct: 79 MFPEVCEMLDEQPLLSAL---KDAYSIDIKTQCYDDLALSGKEKRYPVLFYVCGGGGSPE 135 Query: 61 WGTVLCTDMASIGYVVVSIGHPNSTMYKRKDGRLFNVSEDFSDAIMALSEDPGMQALAGK 120 WGTV+CTD+AS+GYV+VSIGH NSTMYKRKDGRLFNVS+DFS IMA SEDP M ALAGK Sbjct: 136 WGTVICTDLASMGYVLVSIGHQNSTMYKRKDGRLFNVSKDFSGVIMAFSEDPEMLALAGK 195 Query: 121 MEMRPDDETAAQMCRNVLTLPIISRVTEYSELQAEDVRTVADYLYKLDAGEQNSIFEGRL 180 MEMRPDDETA +MC+NVLTLPI+ ++TEYSELQAEDVR VADYL KLD+GE NSIF+ RL Sbjct: 196 MEMRPDDETAIEMCQNVLTLPILDKLTEYSELQAEDVRYVADYLVKLDSGELNSIFKDRL 255 Query: 181 LFDIGMGIIGHSYGGLTTAMVCRDDDRFACGIGLDSGAFGLLGSDLKTPFLLLFREPNYN 240 L DIGMGI+GHSYGG TTA+VCRDD+RFACGIGLDSGAFGL GSDLK PFLLLF EPNYN Sbjct: 256 LLDIGMGIVGHSYGGATTAIVCRDDERFACGIGLDSGAFGLQGSDLKKPFLLLFSEPNYN 315 Query: 241 MNAIIGANNSMETYYFSVDRVAHLDYCDIVFTSVNEQLRGERDAMEMRNLVTDYTKNFFD 300 MNAIIGANNSM+TYYFSVDR AHLDYCDIVFTSVNE+LRGERDAMEMRNLVTDYTKNFFD Sbjct: 316 MNAIIGANNSMDTYYFSVDRAAHLDYCDIVFTSVNEELRGERDAMEMRNLVTDYTKNFFD 375 Query: 301 HYILQKAVSVESLAYDGVDLIKKTSNK 327 HYILQKA SVESLAYDGVDLIKKTSNK Sbjct: 376 HYILQKAASVESLAYDGVDLIKKTSNK 402 >ref|NP_831586.1| Carboxylic ester hydrolase [Bacillus cereus ATCC 14579] gb|AAP08787.1| Carboxylic ester hydrolase [Bacillus cereus ATCC 14579] Length = 456 Score = 77.4 bits (189), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 74/286 (25%), Positives = 114/286 (39%), Gaps = 50/286 (17%) Query: 29 IKTQCYDDLALSGKEKRYPVLFYVCGGGGSPEWGTVLCTDMASIGYVVVSIGHPNSTMYK 88 +K+ + DL LS E YP+L + G G T ++AS GY+V+SI H Sbjct: 191 VKSHSFMDLQLSDSENHYPILLFSHGFNGFRNQNTFQVEELASQGYIVLSIDH------- 243 Query: 89 RKDGRLFNVSEDFSDAIMALSEDPGMQALAGKMEMRPDDETAAQMCRNVLTLPIISRVTE 148 F A P TA V + + Sbjct: 244 -----------TFDAAATVF----------------PGGRTAY-----VQPINLTDEGDS 271 Query: 149 YSELQAEDVRTVADYLYKLDAGEQNSIFEGRLLFDIG-MGIIGHSYGGLTTAMVCRDDDR 207 + +L EDV V + + KL+ ++ F GRL D +G+ GHSYGG T A + D R Sbjct: 272 HIKLWEEDVSFVLNQIEKLNENDETGFFTGRL--DTSRIGMFGHSYGGATAAQILAKDSR 329 Query: 208 FACGIGLDSGAFG--LLGSDLKTPFLLL-FREPN----YNMNAIIGANNSMETYYFSVDR 260 I +D +G L S + PFLL+ EP+ + + G + + Sbjct: 330 VKAAINMDGTLYGEILPESGIGKPFLLMNAEEPDEADPFEVRERYGRGLAGGGMSMVIPH 389 Query: 261 VAHLDYCDIVFTSVNEQLRGERDAMEMRNLVTDYTKNFFDHYILQK 306 H + D+ S Q GE + E+ ++ +++ FFD Y+ QK Sbjct: 390 TDHTSFTDLHLFSPLLQSPGE-NPKEVHRIINEFSLAFFDQYVKQK 434 >gb|EAE24421.1| unknown [environmental sequence] Length = 193 Score = 65.1 bits (157), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 48/171 (28%), Positives = 84/171 (49%), Gaps = 16/171 (9%) Query: 142 IISRVTEYSELQAEDVRTVADYLYKLDAGEQNSIFEGRLLFDIG-MGIIGHSYGGLTTAM 200 + RV E++ +D+R + D +++ +N + + D +GI GHS+GG T+ M Sbjct: 1 MFQRVRSQLEIRMQDIRFLIDRMFQSPFFMENVM----AIADTSKIGIFGHSFGGCTSVM 56 Query: 201 VCRDDDRFACGIGLDSGAFGL----LGSDLKTPFLLL-----FREPNYNMNAIIGANNSM 251 DDR +GLD+ L + DL PF L R NY + +G NNS Sbjct: 57 AGYKDDRIDAVLGLDTYFLPLPKDAIARDLNKPFAHLGQPSWGRSNNYKVMETLGKNNSK 116 Query: 252 ETYYFSVDRVAHLDYCDIV-FTSVNEQL-RGERDAMEMRNLVTDYTKNFFD 300 +++F+V+ H DY D FT + ++ G+ ++R+++ +FF+ Sbjct: 117 ISFHFAVEGSRHYDYTDFSQFTRLTKKFGSGKISPKKIRSIMNTIMLDFFN 167 >gb|EAC76171.1| unknown [environmental sequence] Length = 227 Score = 64.3 bits (155), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 50/179 (27%), Positives = 85/179 (47%), Gaps = 20/179 (11%) Query: 151 ELQAEDVRTVADYLYKLDAGEQNSIFEGRLLFDIGMGIIGHSYGGLTTAMVCRDDDRFAC 210 + + D++ + D L K+ +G G L +GI GHS+GG T A+V + D R Sbjct: 30 DTRVSDIQFILDELEKIQSGALRHPLNGYLDLS-RIGITGHSFGGSTAALVAKKDTRVKA 88 Query: 211 GIGLDSGAFGLLGSD-----LKTPFLLLFREPNY---------NMNAIIGANNSMETYYF 256 + L G L D + PFL + R P++ +++ I+ NN+ T++ Sbjct: 89 -VSLIDGWMNPLPQDVIRNGIDQPFLYMGR-PSWVDSDYPSSPSISEIMHNNNNGPTHHI 146 Query: 257 SVDRVAHLDYCDI-VFTSVNEQLR--GERDAMEMRNLVTDYTKNFFDHYILQKAVSVES 312 ++ HL++CD +FT + + L G+ D LV + FFD Y+ QK ++ S Sbjct: 147 TIKNTRHLNFCDAPLFTPIGKYLVEIGDIDRKRSVELVNQLSLEFFDMYLKQKPSALLS 205 >gb|EAK00707.1| unknown [environmental sequence] Length = 295 Score = 61.6 bits (148), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 68/291 (23%), Positives = 116/291 (39%), Gaps = 52/291 (17%) Query: 29 IKTQCYDDLALSGKEKRYPVLFYVCGGGGSPEWGTVLCTDMASIGYVVVSIGHPNSTMYK 88 IKT +L+ +PV+ G G + T L +AS GY V ++ H Sbjct: 21 IKTNSALNLSPDPIAAPFPVVIISHGITGMRQLHTSLAEKLASNGYAVFALDHT------ 74 Query: 89 RKDGRLFNVSEDFSDAIMALSEDPGMQALAGKMEMRPDDETAAQMCRNVLTLPIISRVTE 148 DA + + D + ++ PD ++ I R E Sbjct: 75 -------------YDANITVFPDGSVANYRSEITGHPD------------SVKIRKRQIE 109 Query: 149 YSELQAEDVRTVADYLYKLDAGEQNSIFEGRLLFDIGMGIIGHSYGGLTTAMVCRDDDRF 208 + D+ + D L ++ G G + + +G+ GHS+GG T A+ DDR Sbjct: 110 ---TRVADLSFIVDQLERIQNGALRHPLNGYIDLN-RIGVTGHSFGGSTVALALEYDDRI 165 Query: 209 ACGIGLDSG----AFGLLGSDLKTPFLLLFREPNY---------NMNAIIGANNSMETYY 255 G+DS ++ L PFL + R P++ + + I+ NN+ +++ Sbjct: 166 KAASGIDSWMNPIPKEIIDRGLSKPFLYMGR-PSWVDSDYPSSVSFSEIMHQNNNGPSHH 224 Query: 256 FSVDRVAHLDYCDI-VFTSVNEQLRGERDAMEMR--NLVTDYTKNFFDHYI 303 ++ HL++CD +F+ V + L D R NLV + FFD Y+ Sbjct: 225 ITIKNTRHLNFCDAPLFSPVGKYLVEIGDINRQRSVNLVNRLSLEFFDKYL 275 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.139 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 494,650,514 Number of extensions: 21027055 Number of successful extensions: 45810 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 45811 Number of HSP's successfully gapped: 15 Length of query: 327 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 201 Effective length of database: 415,135,104 Effective search space: 83442155904 Effective search space used: 83442155904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)