BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0490.0014.p (450 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_388578.1| similar to sugar-binding protein [Bacillus subt... 273 6e-72 ref|ZP_00057848.1| COG1653: ABC-type sugar transport system, per... 154 3e-36 ref|NP_384223.1| PUTATIVE PERIPLASMIC BINDING ABC TRANSPORTER PR... 152 2e-35 ref|NP_357484.1| AGR_L_3389p [Agrobacterium tumefaciens] >gi|255... 148 3e-34 ref|NP_533614.1| ABC transporter, substrate binding protein [sug... 148 3e-34 >ref|NP_388578.1| similar to sugar-binding protein [Bacillus subtilis] pir||F69796 sugar-binding protein homolog yesO - Bacillus subtilis emb|CAB12516.1| yesO [Bacillus subtilis subsp. subtilis str. 168] Length = 412 Score = 273 bits (698), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 143/301 (47%), Positives = 195/301 (64%), Gaps = 7/301 (2%) Query: 94 VTLRIAWWGGDTRHSYTQQVIDMYEAQNPNVKIEPEYASFDDYWKKLAPQAAANRLPDIV 153 VTLRIAWWGG RH YT +VI++YE +NP+V IE E+A++DDYWKKLAP +AA +LPD++ Sbjct: 18 VTLRIAWWGGQPRHDYTTKVIELYEKKNPHVHIEAEFANWDDYWKKLAPMSAAGQLPDVI 77 Query: 154 QMDVSYINQYGSNGQLEDMTPYL-NKTVQVGDVNENVLGTGKIDGKQYGIPLGVNVLGFH 212 QMD +Y+ QYG QLED+TPY + T+ V ++EN+L GKID K YG LGVNVL Sbjct: 78 QMDTAYLAQYGKKNQLEDLTPYTKDGTIDVSSIDENMLSGGKIDNKLYGFTLGVNVLSVI 137 Query: 213 YDPALLKKAGVDSIPDNWTWDQYKEIAMK-AKAAGLYMDSSM-AADIFFNYYLRTKGLSL 270 + LLKKAGV +NWTW+ Y+++A + AG+Y + M DIFF YYLRTKG Sbjct: 138 ANEDLLKKAGVSINQENWTWEDYEKLAYDLQEKAGVYGSNGMHPPDIFFPYYLRTKGERF 197 Query: 271 YNTDXXXXSALGYDDDALFRDFFGMLSDLIAKGAVPSQDKLSQNKGVIEEADVVKGTGIG 330 Y D + L Y DD LF D+F L+ P+ D+ +Q KG +E+ +VKG Sbjct: 198 YKED---GTGLAYQDDQLFVDYFERQLRLVKAKTSPTPDESAQIKG-MEDDFIVKGKSAI 253 Query: 331 IWQWSNQYVALQIAVNRPMELAQMPGPDMEKGLYMQPSMYWSVTANSKNKEEAAKFIDFW 390 W +SNQY+ + P+ L P EK L ++PSM +S+ +S++K+EAAKFI+F+ Sbjct: 254 TWNYSNQYLGFARLTDSPLSLYLPPEQMQEKALTLKPSMLFSIPKSSEHKKEAAKFINFF 313 Query: 391 T 391 Sbjct: 314 V 314 >ref|ZP_00057848.1| COG1653: ABC-type sugar transport system, periplasmic component [Thermobifida fusca] Length = 448 Score = 154 bits (390), Expect = 3e-36, Method: Compositional matrix adjust. Identities = 104/305 (34%), Positives = 160/305 (52%), Gaps = 17/305 (5%) Query: 94 VTLRIAWWGGDTRHSYTQQVIDMYEAQNPNVKIEPEYASFDDYWKKLAPQAAANRLPDIV 153 V LR +WWG D RH+ TQ+VI+++E QNP +KI EY + YW KLA AA PDI+ Sbjct: 53 VELRFSWWGSDERHAMTQEVIELFEEQNPGIKIVGEYTDWASYWDKLATTTAAGDAPDII 112 Query: 154 QMDVSYINQYGSNGQLEDMTPYLNKTVQVGDVNENVLGTGKIDGKQYGIPLGVNVLGFHY 213 + Y+ +Y G L D++ + + ++ V +G +DG YG+ GVN Sbjct: 113 TQEERYLREYAERGALLDLSQL--DGLDLSKIDPLVAESGDLDGATYGVATGVNAYAILA 170 Query: 214 DPALLKKAGVDSIP--DNWTWDQYKEIA--MKAKAAGLYMDSSMAA--DIFFNYYLRTKG 267 DP AGVD +P D WTWD Y +IA + K+ G + + + F + R G Sbjct: 171 DPQAFADAGVD-MPDDDTWTWDDYVKIAAEISEKSNGEIVGTQAMGYNETGFAIFARQHG 229 Query: 268 LSLYNTDXXXXSALGYDDDALFRDFFGMLSDLIAKGAVP-SQDKLSQNKGVIEEADVVKG 326 SLY D LG+ L + +F DL+ GA P + + + G +++ + Sbjct: 230 ESLYTED----GKLGFSKKTLEK-WFQYTVDLLESGAQPGASESVEIEAGGPDQSVLATN 284 Query: 327 TGIGIWQWSNQYVALQIAVNRPMELAQMPGPDMEK--GLYMQPSMYWSVTANSKNKEEAA 384 G W+NQ A+ + R +EL + PG + G+Y +P+MY+S++A +++ EEAA Sbjct: 285 KGAMAHFWTNQLGAISASSGREIELLRYPGETEHERTGMYFKPAMYYSISAGTEHPEEAA 344 Query: 385 KFIDF 389 KF+DF Sbjct: 345 KFVDF 349 >ref|NP_384223.1| PUTATIVE PERIPLASMIC BINDING ABC TRANSPORTER PROTEIN [Sinorhizobium meliloti] emb|CAC41504.1| PUTATIVE PERIPLASMIC BINDING ABC TRANSPORTER PROTEIN [Sinorhizobium meliloti] Length = 427 Score = 152 bits (383), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 95/304 (31%), Positives = 151/304 (49%), Gaps = 14/304 (4%) Query: 96 LRIAWWGGDTRHSYTQQVIDMYEAQNPNVKIEPEYASFDDYWKKLAPQAAANRLPDIVQM 155 LR+ WWG R T +V +++ QN V I E+ + DYW +LA Q A PDI+QM Sbjct: 33 LRVLWWGSQARADRTNKVNQLFQEQNAGVAINGEFLGWSDYWPRLATQVAGRNAPDIIQM 92 Query: 156 DVSYINQYGSNGQLEDMTPYLNKTVQVGDVNENVLGTGKIDGKQYGIPLGVNVLGFHYDP 215 D YI +Y G L + YL ++V D ++ + G ++GK YGI LG N + Sbjct: 93 DYRYIVEYARRGALAPLDDYLGSVLKVEDFDQVQIKGGSVEGKLYGISLGANSAAMMVNA 152 Query: 216 ALLKKAGVDSIPDNWTWDQY----KEIAMKAKAAGLY-MDSSMAADIFFNYYLRTKGLSL 270 A ++AG++ + TWD+ EI K G Y + A + F +LR +G +L Sbjct: 153 AAFEEAGIELPTPSTTWDEMARIGAEITKAGKRKGFYGLSDGSAVEPLFENWLRQRGKAL 212 Query: 271 YNTDXXXXSALGYD-DDALFRDFFGMLSDLIAKGAVPSQDKLSQNKGVIEEADVVKGTGI 329 + D + YD +DA ++F M + A D + ++ +E + + G Sbjct: 213 FTAD----GKIAYDANDAA--EWFAMWQKMREDKACVPPDIQALDQYNVETSPLSLGKAA 266 Query: 330 GIWQWSNQYVALQIAVNRPMELAQMP--GPDMEKGLYMQPSMYWSVTANSKNKEEAAKFI 387 + SNQ+VA Q + L P G D + G Y +PSM++SV+A +K+ E AK++ Sbjct: 267 ASFAHSNQFVAYQGINKDKLALRSHPLIGKDAKGGHYRKPSMFFSVSAQTKDPELGAKYV 326 Query: 388 DFWT 391 +F+ Sbjct: 327 NFFV 330 >ref|NP_357484.1| AGR_L_3389p [Agrobacterium tumefaciens] pir||C98343 sugar-binding protein homolog yesO [imported] - Agrobacterium tumefaciens (strain C58, Cereon) gb|AAK90269.1| AGR_L_3389p [Agrobacterium tumefaciens str. C58 (Cereon)] Length = 438 Score = 148 bits (373), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 97/301 (32%), Positives = 148/301 (49%), Gaps = 12/301 (3%) Query: 96 LRIAWWGGDTRHSYTQQVIDMYEAQNPNVKIEPEYASFDDYWKKLAPQAAANRLPDIVQM 155 LR+ WWG R T + I Y+ P++ I E+A + DYW +LA Q A PD++QM Sbjct: 44 LRMLWWGSQERADRTNKAISAYKQVKPDLDIAGEFAGWSDYWPRLATQVAGRNAPDLIQM 103 Query: 156 DVSYINQYGSNGQLEDMTPYLNKTVQVGDVNENVLGTGKIDGKQYGIPLGVNVLGFHYDP 215 D YI +YG G L + Y+ K +++ D + + +G++DGK YGI LGVN +D Sbjct: 104 DYRYIFEYGRRGALAPLDDYIGKGLKIEDFGKMNIDSGRVDGKLYGINLGVNSSAVFFDK 163 Query: 216 ALLKKAGVDSIPDNWTWDQYKEIAM---KAKAAGLYMDSSMAADI--FFNYYLRTKGLSL 270 A +K+G TW+++ E A K K Y ++ A+ + F +LR G SL Sbjct: 164 AAWEKSGATPPATGQTWEEFAENAARLSKNKPKPGYYATADASGVEPSFENWLRQNGKSL 223 Query: 271 YNTDXXXXSALGYDDDALFRDFFGMLSDLIAKGAVPSQDKLSQNKGVIEEADVVKGTGIG 330 Y D A+G+D + +F + +DL GA D + ++ IE + G Sbjct: 224 YTQD----GAIGFDPTDATK-WFSLWADLRKSGACVPADVQALDQLNIETNPLTTGKAAT 278 Query: 331 IWQWSNQYVALQIAVNRPMELAQMPGP--DMEKGLYMQPSMYWSVTANSKNKEEAAKFID 388 + SNQ+V Q + ++ P D G Y++PSM SV S E+A FI+ Sbjct: 279 AFAHSNQFVGYQKLNKSKLAISSYPKSKKDGPSGHYLKPSMLISVANGSAGIEQAVGFIN 338 Query: 389 F 389 F Sbjct: 339 F 339 >ref|NP_533614.1| ABC transporter, substrate binding protein [sugar] [Agrobacterium tumefaciens str. C58 (U. Washington)] pir||AD2939 hypothetical protein Atu3114 [imported] - Agrobacterium tumefaciens (strain C58, Dupont) gb|AAL43930.1| ABC transporter, substrate binding protein [sugar] [Agrobacterium tumefaciens str. C58 (U. Washington)] Length = 429 Score = 148 bits (373), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 97/301 (32%), Positives = 148/301 (49%), Gaps = 12/301 (3%) Query: 96 LRIAWWGGDTRHSYTQQVIDMYEAQNPNVKIEPEYASFDDYWKKLAPQAAANRLPDIVQM 155 LR+ WWG R T + I Y+ P++ I E+A + DYW +LA Q A PD++QM Sbjct: 35 LRMLWWGSQERADRTNKAISAYKQVKPDLDIAGEFAGWSDYWPRLATQVAGRNAPDLIQM 94 Query: 156 DVSYINQYGSNGQLEDMTPYLNKTVQVGDVNENVLGTGKIDGKQYGIPLGVNVLGFHYDP 215 D YI +YG G L + Y+ K +++ D + + +G++DGK YGI LGVN +D Sbjct: 95 DYRYIFEYGRRGALAPLDDYIGKGLKIEDFGKMNIDSGRVDGKLYGINLGVNSSAVFFDK 154 Query: 216 ALLKKAGVDSIPDNWTWDQYKEIAM---KAKAAGLYMDSSMAADI--FFNYYLRTKGLSL 270 A +K+G TW+++ E A K K Y ++ A+ + F +LR G SL Sbjct: 155 AAWEKSGATPPATGQTWEEFAENAARLSKNKPKPGYYATADASGVEPSFENWLRQNGKSL 214 Query: 271 YNTDXXXXSALGYDDDALFRDFFGMLSDLIAKGAVPSQDKLSQNKGVIEEADVVKGTGIG 330 Y D A+G+D + +F + +DL GA D + ++ IE + G Sbjct: 215 YTQD----GAIGFDPTDATK-WFSLWADLRKSGACVPADVQALDQLNIETNPLTTGKAAT 269 Query: 331 IWQWSNQYVALQIAVNRPMELAQMPGP--DMEKGLYMQPSMYWSVTANSKNKEEAAKFID 388 + SNQ+V Q + ++ P D G Y++PSM SV S E+A FI+ Sbjct: 270 AFAHSNQFVGYQKLNKSKLAISSYPKSKKDGPSGHYLKPSMLISVANGSAGIEQAVGFIN 329 Query: 389 F 389 F Sbjct: 330 F 330 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 654,630,808 Number of extensions: 26780672 Number of successful extensions: 54625 Number of sequences better than 1.0e-05: 129 Number of HSP's gapped: 54599 Number of HSP's successfully gapped: 129 Length of query: 450 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 321 Effective length of database: 406,989,807 Effective search space: 130643728047 Effective search space used: 130643728047 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)