BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0019.p (195 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00097594.1| COG0178: Excinuclease ATPase subunit [Desulfi... 192 2e-48 ref|ZP_00081549.1| COG0178: Excinuclease ATPase subunit [Geobact... 188 5e-47 ref|NP_954365.1| excinuclease ABC, A subunit [Geobacter sulfurre... 187 1e-46 ref|NP_618214.1| excinuclease ABC, subunit A [Methanosarcina ace... 186 2e-46 ref|NP_966656.1| excinuclease ABC, A subunit [Wolbachia endosymb... 184 7e-46 >ref|ZP_00097594.1| COG0178: Excinuclease ATPase subunit [Desulfitobacterium hafniense] Length = 823 Score = 192 bits (489), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 98/198 (49%), Positives = 136/198 (68%), Gaps = 9/198 (4%) Query: 2 CNGYGHIKRWLSQLVFSEE----CPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAF 57 C G G++ +S ++F E+ CP C G +F+DEVL VKY+ SI D+L MSV EA A Sbjct: 630 CEGLGYV---VSNMLFFEDLEVSCPVCHGDQFQDEVLSVKYQGHSIKDVLGMSVEEALAV 686 Query: 58 FQDQPKISSLLTAIEEMGMGYIHLGQSLTTLSGGEVQRLKLAREL-GKSGKVNYLYILDE 116 F P+I+ +L +EE+G+GY+ LGQ+LTTLSGGE QRLKLA+EL G GK N LY++DE Sbjct: 687 FALHPRITKVLELLEEVGLGYLELGQTLTTLSGGEGQRLKLAKELIGNEGKTN-LYLIDE 745 Query: 117 PTVGLSYYDAEKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIVAT 176 PT GL D E L +L+++V +VI++EH+ V+ D++I+LGP GHKGG ++A Sbjct: 746 PTTGLHPLDVENFLALLNRMVDAGSTVIVVEHNQQVMQASDWIIDLGPEGGHKGGRVMAV 805 Query: 177 GTPEEVKKHAGSMIGPFL 194 GTPE++K S+ G FL Sbjct: 806 GTPEQIKSEPDSVTGRFL 823 Score = 112 bits (280), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 69/202 (34%), Positives = 110/202 (54%), Gaps = 17/202 (8%) Query: 10 RWLSQLVFSEECPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFF---------QD 60 R LS + CP C G+R V + ++ ++S+ E Q + Sbjct: 288 RGLSAYFAEQICPDCAGERLNGLSRSVTVLGTRLPELASLSLEELQDWLIKLEDSVTGTH 347 Query: 61 QPKISSLLTAIE-------EMGMGYIHLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYI 113 Q ++S L ++ ++G+GY+ L + TLSGGE QR+KLA L S +YI Sbjct: 348 QDLVASYLNDLKTKIRRIIKVGLGYLSLDRQTMTLSGGEAQRIKLAATL-DSTLTGIIYI 406 Query: 114 LDEPTVGLSYYDAEKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEI 173 +DEPT+GL D + ++ IL +L ++VI+IEHD V+S D+++++GPGAG GGEI Sbjct: 407 MDEPTIGLHPQDTQGIIQILKELRDLGNTVIVIEHDMDVMSAADFIVDIGPGAGKLGGEI 466 Query: 174 VATGTPEEVKKHAGSMIGPFLE 195 V G+ +E+K+ S+ G +L Sbjct: 467 VGQGSIDELKEQGRSVTGLYLN 488 >ref|ZP_00081549.1| COG0178: Excinuclease ATPase subunit [Geobacter metallireducens] Length = 952 Score = 188 bits (478), Expect = 5e-47, Method: Compositional matrix adjust. Identities = 96/196 (48%), Positives = 132/196 (67%), Gaps = 5/196 (2%) Query: 2 CNGYGHIK---RWLSQLVFSEECPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFF 58 C+G G IK +L + +C C+G R+ E L V YK +SIA +L M+V+EA F Sbjct: 755 CSGDGIIKIEMHFLPDVYV--QCEVCKGARYNRETLEVTYKGKSIAQVLDMTVSEALRFL 812 Query: 59 QDQPKISSLLTAIEEMGMGYIHLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYILDEPT 118 ++ PKI + L +EE+G+GYI LGQ+ TTLSGGE QR+KLA+EL + +YILDEPT Sbjct: 813 ENIPKIKTKLQTLEEVGLGYIRLGQAATTLSGGEAQRVKLAKELARRATGRTIYILDEPT 872 Query: 119 VGLSYYDAEKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIVATGT 178 GL ++D KLL +L KLV G +++++IEH+ V+ DYLI+LGP G +GGE++ATGT Sbjct: 873 TGLHFHDIAKLLEVLRKLVEGGNTIVIIEHNLDVIKTADYLIDLGPEGGDRGGEVIATGT 932 Query: 179 PEEVKKHAGSMIGPFL 194 PEEV K S G +L Sbjct: 933 PEEVAKVTRSYTGQYL 948 Score = 127 bits (320), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 70/188 (37%), Positives = 107/188 (56%), Gaps = 15/188 (7%) Query: 21 CPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFFQD---QPK-----------ISS 66 CP+C+G R K E L VK R I + A+S+ +A +F PK I Sbjct: 421 CPTCEGARLKKEALHVKVAGRDIRQVTALSIKDALEYFASLALTPKEEEIARRILKEIRE 480 Query: 67 LLTAIEEMGMGYIHLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYILDEPTVGLSYYDA 126 L + +G+ Y+ L ++ TLSGGE QR++LA ++G S V LYILDEP++GL D Sbjct: 481 RLNFLVNVGLDYLSLDRASGTLSGGEGQRIRLATQIG-SSLVGVLYILDEPSIGLHQRDN 539 Query: 127 EKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIVATGTPEEVKKHA 186 +LL L + ++V+++EHD + D+++++GPGAG GG +VA GTP E+ + Sbjct: 540 GRLLQTLKHMRDIGNTVLVVEHDEETILEADHVLDMGPGAGEHGGRVVAQGTPAEIMANP 599 Query: 187 GSMIGPFL 194 S+ G +L Sbjct: 600 ASLTGRYL 607 >ref|NP_954365.1| excinuclease ABC, A subunit [Geobacter sulfurreducens PCA] gb|AAR36715.1| excinuclease ABC, A subunit [Geobacter sulfurreducens PCA] Length = 937 Score = 187 bits (475), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 89/175 (50%), Positives = 123/175 (70%) Query: 20 ECPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFFQDQPKISSLLTAIEEMGMGYI 79 +C C+G R+ E L V YK +SIA +L M+V+EA F ++ PK+ + L +EE+G+GYI Sbjct: 759 QCEVCKGARYNRETLEVTYKGKSIAQVLDMTVSEALRFLENIPKVKAKLQTLEEVGLGYI 818 Query: 80 HLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYILDEPTVGLSYYDAEKLLMILSKLVAG 139 LGQS TTLSGGE QR+KLA+EL + +YILDEPT GL ++D KLL +L KLV G Sbjct: 819 RLGQSATTLSGGEAQRVKLAKELARRATGRTIYILDEPTTGLHFHDIAKLLEVLRKLVEG 878 Query: 140 KHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIVATGTPEEVKKHAGSMIGPFL 194 +++++IEH+ V+ DY+I+LGP G +GGE++ATGTPEEV K S G +L Sbjct: 879 GNTIVIIEHNLDVIKTADYIIDLGPEGGDRGGEVIATGTPEEVAKVTRSYTGQYL 933 Score = 130 bits (328), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 72/188 (38%), Positives = 110/188 (58%), Gaps = 15/188 (7%) Query: 21 CPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFF-------QDQPKISSLLTAIEE 73 CP+CQG R K E L VK +R I + A+S+ +A FF +++ +L I E Sbjct: 406 CPTCQGARLKREALHVKVAERDIRQVTALSIKDALEFFASLTLTPKEEEIARRILKEIRE 465 Query: 74 -------MGMGYIHLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYILDEPTVGLSYYDA 126 +G+ Y+ L ++ TLSGGE QR++LA ++G S V LYILDEP++GL D Sbjct: 466 RLHFLVNVGLDYLSLDRTSGTLSGGEGQRIRLATQIG-SSLVGVLYILDEPSIGLHQRDN 524 Query: 127 EKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIVATGTPEEVKKHA 186 +LL L L ++V+++EHD + D+++++GPGAG GG +VA GTP E+ + Sbjct: 525 GRLLQTLKHLRDIGNTVLVVEHDEETILEADHVLDMGPGAGEHGGRVVAQGTPAEIMANP 584 Query: 187 GSMIGPFL 194 S+ G +L Sbjct: 585 ESLTGRYL 592 >ref|NP_618214.1| excinuclease ABC, subunit A [Methanosarcina acetivorans str. C2A] gb|AAM06694.1| excinuclease ABC, subunit A [Methanosarcina acetivorans str. C2A] Length = 993 Score = 186 bits (472), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 96/196 (48%), Positives = 129/196 (65%), Gaps = 5/196 (2%) Query: 2 CNGYGHIK---RWLSQLVFSEECPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFF 58 C G G IK +L + EC C+G R+ E L VKYK +SIA++L M+V EA F Sbjct: 736 CQGDGMIKIEMNFLPDVYI--ECEECKGTRYNRETLEVKYKGKSIAEVLNMTVEEAAEHF 793 Query: 59 QDQPKISSLLTAIEEMGMGYIHLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYILDEPT 118 ++ P I L + +G+GYI LGQS TTLSGGE QR+KL REL K G +Y+LDEPT Sbjct: 794 ENVPAIKGKLDTLTRVGLGYIKLGQSSTTLSGGEAQRIKLTRELSKKGTGKTIYLLDEPT 853 Query: 119 VGLSYYDAEKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIVATGT 178 GL ++D +KL+ +L+ LVA ++V++IEH+ V+ D++I+LGP GH GGEIVATGT Sbjct: 854 TGLHFHDVKKLISVLNGLVAKGNTVVVIEHNLDVIKSADHIIDLGPEGGHAGGEIVATGT 913 Query: 179 PEEVKKHAGSMIGPFL 194 PEE+ + S G FL Sbjct: 914 PEEIARIPESHTGRFL 929 Score = 140 bits (353), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 74/200 (37%), Positives = 120/200 (60%), Gaps = 15/200 (7%) Query: 9 KRWLSQLVFSEECPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFFQDQP------ 62 ++ L + + CP C+GKR K++VL VK +SI D +S+ + FF++ Sbjct: 390 RKELEKFMQVRPCPRCEGKRLKEKVLAVKLAGKSIVDATDLSIIQCIQFFEELKLSEKEQ 449 Query: 63 --------KISSLLTAIEEMGMGYIHLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYIL 114 +I S L +E +G+GY+ L +S TLSGGE QR++LA ++G S + LY+L Sbjct: 450 EIAKQVLKEIHSRLGFLEHVGLGYLTLSRSAGTLSGGEAQRIRLATQIG-SNLMGVLYVL 508 Query: 115 DEPTVGLSYYDAEKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIV 174 DEP++GL D E+L+ L L +++I++EHD + DY++++GPGAG GG +V Sbjct: 509 DEPSIGLHQRDNERLIQTLQTLRDLGNTLIVVEHDEDTIRAADYVLDIGPGAGIHGGYVV 568 Query: 175 ATGTPEEVKKHAGSMIGPFL 194 A GTP E++++ S+ G +L Sbjct: 569 AEGTPAEIERNPESLTGKYL 588 >ref|NP_966656.1| excinuclease ABC, A subunit [Wolbachia endosymbiont of Drosophila melanogaster] gb|AAS14590.1| excinuclease ABC, A subunit [Wolbachia endosymbiont of Drosophila melanogaster] Length = 931 Score = 184 bits (468), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 93/197 (47%), Positives = 134/197 (68%), Gaps = 5/197 (2%) Query: 2 CNGYGHIK---RWLSQLVFSEECPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFF 58 C G GH+K +L + +C C+G+R+ E L V YK++SI+D+L M++ +A FF Sbjct: 733 CKGDGHLKIEMHFLPDVYV--KCEQCKGRRYNRETLEVTYKEKSISDVLDMTIDQACDFF 790 Query: 59 QDQPKISSLLTAIEEMGMGYIHLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYILDEPT 118 ++ P + L +++E+G+GYI LGQS TTLSGGE QR+KL++EL K LYILDEPT Sbjct: 791 ENLPMVKEKLVSLQEVGLGYIKLGQSSTTLSGGEAQRIKLSKELSKRFTGRTLYILDEPT 850 Query: 119 VGLSYYDAEKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIVATGT 178 GL + D LL IL +LV ++VI+IEH+ +V+ DY+I++GP G KGGE++ATGT Sbjct: 851 TGLHFEDINNLLKILHRLVNLGNTVIVIEHNLHVIKTADYIIDIGPEGGIKGGEVIATGT 910 Query: 179 PEEVKKHAGSMIGPFLE 195 PEEV K S+ G +L+ Sbjct: 911 PEEVVKIPESVTGRYLK 927 Score = 137 bits (344), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 71/190 (37%), Positives = 114/190 (60%), Gaps = 17/190 (8%) Query: 21 CPSCQGKRFKDEVLRVKYKQRSIADILAMSVTEAQAFFQDQP----------------KI 64 C C G R + E L VK + I +I +S+ E+ +F++ P +I Sbjct: 397 CRECTGYRLRKEALTVKIDSKHIGEISGLSIDESLKWFENLPDKLTEQQKQISNKILSEI 456 Query: 65 SSLLTAIEEMGMGYIHLGQSLTTLSGGEVQRLKLARELGKSGKVNYLYILDEPTVGLSYY 124 LT ++ +G+ Y+ L + +TLSGGE QRL+LA ++G SG LY+LDEP++GL Sbjct: 457 IKRLTFLKNVGLNYLTLDRESSTLSGGESQRLRLASQIG-SGLTGVLYVLDEPSIGLHQC 515 Query: 125 DAEKLLMILSKLVAGKHSVILIEHDPYVLSYCDYLIELGPGAGHKGGEIVATGTPEEVKK 184 D ++L+ L L ++VI++EHD + DY I++GPGAG GG++VA GTP++V++ Sbjct: 516 DNDRLIATLKNLRDMGNTVIVVEHDEDTIMAADYAIDIGPGAGVNGGKVVAEGTPDQVQR 575 Query: 185 HAGSMIGPFL 194 ++GS+ G +L Sbjct: 576 NSGSITGQYL 585 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.138 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 295,501,322 Number of extensions: 11460809 Number of successful extensions: 54613 Number of sequences better than 1.0e-05: 1233 Number of HSP's gapped: 55951 Number of HSP's successfully gapped: 1592 Length of query: 195 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 76 Effective length of database: 434,140,797 Effective search space: 32994700572 Effective search space used: 32994700572 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 122 (51.6 bits)