BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0014.p (218 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO61214.1| phosphatase [Streptomyces hygroscopicus] 124 1e-27 gb|AAD31830.1| NapH phosphatase [Streptomyces collinus] 124 2e-27 gb|AAD31837.1| AnsH phosphatase [Streptomyces collinus] 119 6e-26 gb|AAD28462.1| MitJ [Streptomyces lavendulae] 117 3e-25 gb|AAN63810.1| ShnP [Streptomyces hygroscopicus] 114 2e-24 >gb|AAO61214.1| phosphatase [Streptomyces hygroscopicus] Length = 331 Score = 124 bits (312), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 71/202 (35%), Positives = 107/202 (52%), Gaps = 2/202 (0%) Query: 3 NAVVFDFDGVIIDSLEVQRKAFFESF-RLVSDGPLPSFEEFLGHSGDSIPNIFTKMGLSL 61 +VVFD DGV+++S V R+AF ++ +V DG P FEE+ H G P+I MGL L Sbjct: 121 TSVVFDLDGVLVNSFAVMREAFTLAYAEVVGDG-EPPFEEYNRHLGRYFPDIMRIMGLPL 179 Query: 62 DMTEHYRRIARENIHLIQIIDGMEELLRYIKDAGWLCGLCTGKDRPRTLLTLGEHGLAGY 121 +M + R + HL+++ DG+ ELL ++ G + TGK PR L G+ G Sbjct: 180 EMEGPFVRESYRLAHLVEMFDGVPELLSELRHRGLRLAVATGKSGPRARSLLDTLGIRGQ 239 Query: 122 FDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIICAREAKMRSIAVAWG 181 F V+ SD+V PKP P+ +L +D + + +V VGD D+ AR A + ++A WG Sbjct: 240 FHVVLGSDEVARPKPAPDIVLKAMDMMDADPDRTVMVGDAVTDLASARGAGITAVAAMWG 299 Query: 182 HNPVEILRQEAPSYVAYNVEEL 203 + L P + + EL Sbjct: 300 ETDEKTLLAAEPDVILHKPAEL 321 >gb|AAD31830.1| NapH phosphatase [Streptomyces collinus] Length = 231 Score = 124 bits (311), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 72/182 (39%), Positives = 103/182 (56%), Gaps = 6/182 (3%) Query: 2 NNAVVFDFDGVIIDSLEVQRKAFFESF-RLVSDGPLPSFEEFLGHSGDSIPNIFTKMGLS 60 AVVFD DGVI+DS V R+AF ++ +V DGP P FEE++ H G P+I MGL Sbjct: 20 RRAVVFDLDGVIVDSFAVMREAFAVAYAEVVGDGPAP-FEEYVRHQGRYFPDIMRIMGLP 78 Query: 61 LDMTEHYRRIARENIHLIQIIDGMEELLRYIKDAGWLCGLCTGK--DRPRTLLTLGEHGL 118 L+M E + R + H +++ DG+ ELL ++ G + TGK DR R+LL GL Sbjct: 79 LEMEEPFVRESYRLAHRVEVFDGVLELLLTLRVRGLRLAVATGKAGDRARSLLD--GLGL 136 Query: 119 AGYFDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIICAREAKMRSIAV 178 +F V+ SD+V PKP P+ + ++ L E ++ VGD D+ AR+A + S A Sbjct: 137 LPFFAHVIGSDEVPRPKPAPDIVQRALELLDVPPEQAIMVGDAPTDLASARDAGVASAAA 196 Query: 179 AW 180 W Sbjct: 197 LW 198 >gb|AAD31837.1| AnsH phosphatase [Streptomyces collinus] Length = 268 Score = 119 bits (297), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 73/202 (36%), Positives = 106/202 (52%), Gaps = 2/202 (0%) Query: 3 NAVVFDFDGVIIDSLEVQRKAFFESF-RLVSDGPLPSFEEFLGHSGDSIPNIFTKMGLSL 61 +AVVFD DGV+++S V R+AF ++ +V DG P FEE+ H G P+I MGL Sbjct: 57 SAVVFDLDGVLVNSFAVMRQAFTTAYAEVVGDGDAP-FEEYERHLGRYFPDIMRIMGLPP 115 Query: 62 DMTEHYRRIARENIHLIQIIDGMEELLRYIKDAGWLCGLCTGKDRPRTLLTLGEHGLAGY 121 +M + R + HL+ + DG+ E+L ++ G + TGK PR L + GL Sbjct: 116 EMEAPFVRESYRLAHLVPVFDGVPEMLGQLRARGVGLAVATGKSGPRARSLLEQLGLLHL 175 Query: 122 FDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIICAREAKMRSIAVAWG 181 F V+ SD+V PKP P+ +L + L + +V VGD D+ AR+A + ++A WG Sbjct: 176 FTVVIGSDEVRRPKPAPDIVLKALGVLGSDPSRTVMVGDAVTDLASARDAGVAAVAALWG 235 Query: 182 HNPVEILRQEAPSYVAYNVEEL 203 E L E P V EL Sbjct: 236 ETDEETLLAEKPDVVLRTPAEL 257 >gb|AAD28462.1| MitJ [Streptomyces lavendulae] Length = 235 Score = 117 bits (292), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 67/194 (34%), Positives = 95/194 (48%) Query: 3 NAVVFDFDGVIIDSLEVQRKAFFESFRLVSDGPLPSFEEFLGHSGDSIPNIFTKMGLSLD 62 VVFD DGV+IDS V R+AF ++R V P FEE+ H G P+I MGL + Sbjct: 25 TTVVFDLDGVLIDSFAVMREAFAVAYREVVGPGEPPFEEYRTHQGRYFPDIMRLMGLPGE 84 Query: 63 MTEHYRRIARENIHLIQIIDGMEELLRYIKDAGWLCGLCTGKDRPRTLLTLGEHGLAGYF 122 M E + R + + +++ + +LL ++ G + TGK R L GL Sbjct: 85 MEEPFVRESHRLMDRVEVYPDVPQLLAELRADGVGTAIATGKSGSRARAVLEAVGLLPLL 144 Query: 123 DAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIICAREAKMRSIAVAWGH 182 D VV SD+V PKPHP+ + + +L E +V VGD DI R A ++ WG Sbjct: 145 DEVVGSDEVPRPKPHPDIVREALRRLDAAPEDAVMVGDAVIDIRSGRAAGTATVGATWGE 204 Query: 183 NPVEILRQEAPSYV 196 LR E P ++ Sbjct: 205 GAAGQLRAERPDFL 218 >gb|AAN63810.1| ShnP [Streptomyces hygroscopicus] Length = 232 Score = 114 bits (284), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 65/183 (35%), Positives = 102/183 (55%), Gaps = 6/183 (3%) Query: 2 NNAVVFDFDGVIIDSLEVQRKAFFESF-RLVSDGPLPSFEEFLGHSGDSIPNIFTKMGLS 60 +AV+FD DGV++DS EV +AF ++ +V G P FEE+ H G P+I MGL Sbjct: 21 RHAVIFDLDGVVVDSFEVMGEAFSLAYAEVVGTGEAP-FEEYRRHQGRYFPDIMRIMGLP 79 Query: 61 LDMTEHYRRIARENIHLIQIIDGMEELLRYIKDAGWLCGLCTGK--DRPRTLLTLGEHGL 118 L+M E + R + +Q+ DG+ ++LR + + G + TGK +R R+LL + GL Sbjct: 80 LEMEEPFVRESYRLADRVQVYDGVVDVLRTLNERGLRLAIATGKAGERARSLLDV--LGL 137 Query: 119 AGYFDAVVCSDDVTHPKPHPESLLCTIDQLHTTVEHSVFVGDGKNDIICAREAKMRSIAV 178 YF V+ SD+V PKP P+ + ++ L E ++ +GD D+ A A + ++A Sbjct: 138 LPYFAHVIGSDEVPRPKPAPDIIRRALELLEVPAERAIMIGDAPTDLASAHGADVTAVAA 197 Query: 179 AWG 181 WG Sbjct: 198 LWG 200 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.140 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 342,507,923 Number of extensions: 14132512 Number of successful extensions: 30456 Number of sequences better than 1.0e-05: 306 Number of HSP's gapped: 30377 Number of HSP's successfully gapped: 306 Length of query: 218 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 97 Effective length of database: 428,710,599 Effective search space: 41584928103 Effective search space used: 41584928103 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)