BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0010.p (425 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||T44805 malonyl-CoA transacylase fenF [imported] - Bacillus ... 239 6e-62 gb|AAA81588.1| transacylase 236 1e-60 emb|CAE11251.1| BmyD protein [Bacillus amyloliquefaciens] 226 6e-58 gb|AAN37954.1| Ita22 [Bacillus sp. CY22] 226 6e-58 dbj|BAB69697.1| putative malonyl-CoA transacylase [Bacillus subt... 226 1e-57 >pir||T44805 malonyl-CoA transacylase fenF [imported] - Bacillus subtilis gb|AAF08794.1| FenF [Bacillus subtilis] Length = 400 Score = 239 bits (611), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 134/395 (33%), Positives = 214/395 (54%), Gaps = 4/395 (1%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M +A LFPGQGSQ+ MGKS + A+ +F +D + D+ + +G++ +LTRT Sbjct: 1 MNNLAFLFPGQGSQFVGMGKSFWNDFVLAKRLFEEASDAISMDVKKLCFDGDMTELTRTM 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 AQPAI S +Q + P F+AGHSLGE +AL CAGV +++ A++L+ +R L Sbjct: 61 NAQPAILTVSVIAYQVYMQEIGIKPHFLAGHSLGEYSALVCAGVLSFQEAVKLIRQRGIL 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ A E G MAAI ++ +++ C +S ++ + VA NS Q VISG + V Sbjct: 121 MQNADPEQLGTMAAITQVYIQPLQDLCTEISTEDFPVGVACMNSDQQHVISGHRQAVEFV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 + ++GA L VSAPFH+ + +++ FQ L WP++SNV A PY++ Sbjct: 181 IKKAERMGANHTYLNVSAPFHSSMMRSASEQFQTALNQYSFRDAEWPIISNVTAIPYNNG 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAF----S 296 +R L M VRW E++ YLL GV IE+GPK+ L L+K+ +H+ A+ + Sbjct: 241 HSVREHLQTHMTMPVRWAESMHYLLLHGVTEVIEMGPKNVLVGLLKKITNHIAAYPLGQT 300 Query: 297 YEEHNQEIMAIIDEQIAEKRPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQALSN 356 + H A +E I R +++ + ++ RN N D Y + ++Q L Sbjct: 301 SDLHLLSDSAERNENIVNLRKKQLNKMMIQSIIARNYNKDAKTYSNLTTPLFPQIQLLKE 360 Query: 357 RLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQ 391 R+E + E SA++++ +I + + + K++P EQ Sbjct: 361 RVERKEVELSAEELEHSIHLCQLICEAKQLPTWEQ 395 >gb|AAA81588.1| transacylase Length = 400 Score = 236 bits (601), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 131/391 (33%), Positives = 203/391 (51%), Gaps = 4/391 (1%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M +A LFPGQGSQ+ MGK + A+ +F +D + +D+ + G++++LT+T Sbjct: 1 MNNLAFLFPGQGSQFVGMGKPFWNDFVLAKRLFEEASDAISFDIKKLCFNGDMNELTKTM 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 AQPAI S FQ + P F+AGHSLGE +AL C G ++ A+ LV +R L Sbjct: 61 NAQPAILTVSVIAFQVYMQEIGIKPRFLAGHSLGEYSALVCTGALSFHDAVTLVRQRGIL 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ A + QG MAA+ L ++E C+++S +E VA NS Q V+SG + V + Sbjct: 121 MQNADPQQQGTMAAVTQLSLQTLQEICSKVSTEECPADVACMNSDQQHVVSGHREAVERV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 + GA L VSAPFH+ + +++ FQ VL WP++SNV A PYS Sbjct: 181 IRMAEEKGAKYTYLNVSAPFHSSMIRSASEQFQTVLHQYSFRDAAWPIISNVTAHPYSSG 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYEE- 299 I L M VRW E+I YLL QGV IE+GP + L+ L+K+ +H+ + + Sbjct: 241 NSINEHLKQHMTMPVRWAESIHYLLLQGVTEVIEMGPNNVLSGLLKKITNHIAPYPLGQT 300 Query: 300 ---HNQEIMAIIDEQIAEKRPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQALSN 356 H A+ ++ + R +++ + V RN N D Y + ++ L Sbjct: 301 SDIHQLSNSAVRNKLVVRLRKKQLNKLMIQTVIARNYNKDSLAYSNMTTPLFTQIHELKE 360 Query: 357 RLEEEGREESADDMKEAIEMLRSVFVTKKVP 387 R+E G E S +++ +I + + + K++P Sbjct: 361 RMERHGDELSEQELEHSIHLCKLICKAKQLP 391 >emb|CAE11251.1| BmyD protein [Bacillus amyloliquefaciens] Length = 400 Score = 226 bits (577), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 129/396 (32%), Positives = 206/396 (52%), Gaps = 6/396 (1%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M +A LFPGQGSQ+ MGK + A+ +F +D + D+ + G++++LT+T Sbjct: 1 MNNLAFLFPGQGSQFVGMGKQFWNDFVLAKRLFEEASDAISLDVKKLCFNGDMNELTKTM 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 AQPAI S FQ + P F+AGHSLGE +AL CAG ++ A+ LV ER L Sbjct: 61 NAQPAILTVSVIAFQVYMQEIGVKPRFLAGHSLGEYSALVCAGALSFRDAVTLVRERGIL 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ A + QG MAA+ L ++E C+++S ++ VA NS Q VISG + V + Sbjct: 121 MQNADPQQQGAMAAVTHLSLQTLQEICSKISTEDFPAGVACMNSEQQHVISGHRQAVERV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 + + GA L VSAPFH+ + +++ FQ VL WP++SNV A+PYS Sbjct: 181 IKMAEEKGAAYTYLNVSAPFHSSMIRSASEQFQTVLHQYSFRDAAWPIISNVTARPYSSG 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYEEH 300 I L M VRW E++ YLL GV IE+GP + L L+++ +H+ + + Sbjct: 241 NSISEHLKQHMTMPVRWTESMHYLLLHGVTEVIEMGPNNVLAGLLRKTTNHIVPYPLGQ- 299 Query: 301 NQEIMAIID-----EQIAEKRPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQALS 355 ++ + + + I R +++ + +V RN N D A Y + ++Q L Sbjct: 300 TSDVPPLSNSAERKKHIVHLRKKQLNKLMIQSVIARNYNKDSAAYSNMTTPLFTQIQELK 359 Query: 356 NRLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQ 391 R++ S +++ +I + + + K++P E+ Sbjct: 360 ERMKRHEDVLSEQELEHSIHLCKLICEAKQLPAWEE 395 >gb|AAN37954.1| Ita22 [Bacillus sp. CY22] Length = 400 Score = 226 bits (577), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 129/396 (32%), Positives = 208/396 (52%), Gaps = 6/396 (1%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M +A LFPGQGSQ+ MGK + A+ +F +D + D+ + G++++LT+T Sbjct: 1 MNNLAFLFPGQGSQFVGMGKPFWNDFVLAKRLFEEASDAISLDVKKLCFNGDMNELTKTM 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 AQPAI S FQ + P F+AGHSLGE +AL CAG +++ A+ LV ER L Sbjct: 61 NAQPAILTVSVIAFQVYMQEIGVKPRFLAGHSLGEYSALVCAGALSFQDAVTLVRERGIL 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ A + QG MAA+ L ++E C+++S ++ VA NS Q VISG + V + Sbjct: 121 MQNADPKQQGAMAAVTHLSLQTLQEICSKVSTEDFPAGVACMNSEQQHVISGHRQAVERV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 + + GA L VSAPFH+ + +++ FQ VL WP++SNV A+PYS Sbjct: 181 IKMAEEKGAAYTYLNVSAPFHSSMIRSASEQFQTVLQRYSFRDAAWPIISNVTARPYSSG 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYEEH 300 I L M+ VRW E++ YLL GV IE+GP + L L+++ +H+ + + Sbjct: 241 NSISEHLKQHMMMPVRWTESMHYLLLHGVTEVIEMGPNNVLAGLLRKTTNHIVPYPLGQ- 299 Query: 301 NQEIMAIID-----EQIAEKRPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQALS 355 ++ + + + I R +++ + +V RN N D A Y + ++Q L Sbjct: 300 TSDVPPLSNSAERKKHIVHLRKKQLNKLMIQSVIARNYNKDSAAYSNMTTPLFTQIQELK 359 Query: 356 NRLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQ 391 R++ S +++ +I + + + K++P E+ Sbjct: 360 ERMKRHEDVLSEQELEHSIHLCKLICEAKQLPAWEE 395 >dbj|BAB69697.1| putative malonyl-CoA transacylase [Bacillus subtilis] Length = 400 Score = 226 bits (575), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 129/396 (32%), Positives = 207/396 (52%), Gaps = 6/396 (1%) Query: 1 MIKIALLFPGQGSQYNMMGKSLYESSAEAREIFSRTNDILGYDLLAVINEGNLDQLTRTE 60 M +A LFPGQGSQ+ MGK + A+ +F +D + D+ + G++++LT+T Sbjct: 1 MNNLAFLFPGQGSQFVGMGKQFWNDFVLAKRLFEEASDAISLDVKKLCFNGDMNELTKTM 60 Query: 61 FAQPAIFAYSYAIFQSIWKGKKFFPSFMAGHSLGEITALTCAGVFTYEAALRLVMERASL 120 AQPAI S FQ + P F+AGHSLGE +AL CAG +++ A+ LV ER L Sbjct: 61 NAQPAILTVSVIAFQVYMQEIGVKPRFLAGHSLGEYSALVCAGALSFQDAVTLVRERGIL 120 Query: 121 MQQATTEGQGGMAAINGLHPLLVEEECARLSNDEDKIVVANYNSSNQVVISGSKALVTQA 180 MQ A + QG MAA+ L ++E C+++S ++ VA NS Q VISG + V + Sbjct: 121 MQNADPQQQGTMAAVTQLSLQTLQEICSKVSTEDFPAGVACMNSEQQHVISGHRQAVERV 180 Query: 181 AENLAKIGAISIPLQVSAPFHTPYLEISNQIFQRVLAATDMSAINWPVLSNVDAKPYSDP 240 + + GA L VSAPFH+ + +++ FQ VL WP++SNV A+PYS Sbjct: 181 IKMAEEKGAAYTYLNVSAPFHSSMIRSASEQFQTVLHRYSFRDAAWPIISNVTARPYSSG 240 Query: 241 EGIRARLSMQMVSAVRWKETIDYLLRQGVNTFIELGPKSALTNLIKRDFSHVRAFSYEEH 300 I L M VRW E++ YLL GV IE+GP + L L+++ +H+ + + Sbjct: 241 NSISEHLKQHMTMPVRWTESMHYLLLHGVTEVIEMGPNNVLAGLLRKTTNHIVPYPLGQ- 299 Query: 301 NQEIMAIID-----EQIAEKRPTLVSRSLAIAVCTRNQNWDEAEYQRGVIEPYRKVQALS 355 ++ + + + I R +++ + +V RN N D A Y + ++Q L Sbjct: 300 TSDVPPLSNSTERKKHIVHLRKKQLNKLMIQSVIARNYNKDSAAYSTMTTPLFTQIQELK 359 Query: 356 NRLEEEGREESADDMKEAIEMLRSVFVTKKVPLEEQ 391 R++ S +++ +I + + + K++P E+ Sbjct: 360 ERMKRHEDVLSEQELEHSIHLCKLICEAKQLPAWEE 395 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 562,249,172 Number of extensions: 21221429 Number of successful extensions: 69845 Number of sequences better than 1.0e-05: 1384 Number of HSP's gapped: 66455 Number of HSP's successfully gapped: 1622 Length of query: 425 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 297 Effective length of database: 409,704,906 Effective search space: 121682357082 Effective search space used: 121682357082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)