BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0009.p (247 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAB69698.1| iturin A synthetase A [Bacillus subtilis] 157 2e-37 emb|CAE11250.1| BmyA protein [Bacillus amyloliquefaciens] 157 2e-37 pir||T44806 mycosubtilin synthetase chain mycA [imported] - Baci... 156 4e-37 gb|AAS47562.1| mixed type I polyketide synthase - peptide synthe... 139 5e-32 ref|NP_940497.1| Putative polyketide synthase [Corynebacterium d... 135 9e-31 >dbj|BAB69698.1| iturin A synthetase A [Bacillus subtilis] Length = 3982 Score = 157 bits (398), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 77/184 (41%), Positives = 117/184 (63%), Gaps = 4/184 (2%) Query: 33 KDIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQA 92 KDIA+IG++ +P A ++W + G SIR+ P SR +D + + ++ + +++ Sbjct: 669 KDIAIIGMSLNVPGASTKSDFWNLLEKGEHSIREYPESRLKDAADYLKSI---QSEFNES 725 Query: 93 AYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTG 152 + + GYLD ID FD +F ++PK A+ MDPNQRLFLQ A+EDAGY G + G+ G Sbjct: 726 QFVKGGYLDEIDRFDYSFFGLAPKTAQFMDPNQRLFLQSAWHAIEDAGYAGGSMNGSRVG 785 Query: 153 VFLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACSS 212 V+ GYS G +Y+ LLS E + GN+ S++ +R+AY+L++ GP++ VDTACSS Sbjct: 786 VYAGYSK-VGYDYERLLSANYPEELHQYIVGNLPSVLASRIAYFLNLKGPAVTVDTACSS 844 Query: 213 SLVA 216 SL A Sbjct: 845 SLAA 848 >emb|CAE11250.1| BmyA protein [Bacillus amyloliquefaciens] Length = 3982 Score = 157 bits (397), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 77/184 (41%), Positives = 117/184 (63%), Gaps = 4/184 (2%) Query: 33 KDIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQA 92 KDIA+IG++ +P A ++W + G SIR+ P SR +D + + ++ + +++ Sbjct: 669 KDIAIIGMSLNVPGASTKSDFWNLLEKGEHSIREYPESRLKDAADYLKSI---QSEFNES 725 Query: 93 AYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTG 152 + + GYLD ID FD +F ++PK A+ MDPNQRLFLQ A+EDAGY G + G+ G Sbjct: 726 QFVKGGYLDEIDRFDYSFFGLAPKAAQFMDPNQRLFLQSAWHAIEDAGYAGGSMNGSRVG 785 Query: 153 VFLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACSS 212 V+ GYS G +Y+ LLS E + GN+ S++ +R+AY+L++ GP++ VDTACSS Sbjct: 786 VYAGYSK-VGYDYERLLSANYPEELHQYIVGNLPSVLASRIAYFLNLKGPAVTVDTACSS 844 Query: 213 SLVA 216 SL A Sbjct: 845 SLAA 848 >pir||T44806 mycosubtilin synthetase chain mycA [imported] - Bacillus subtilis gb|AAF08795.1| MycA [Bacillus subtilis] Length = 3971 Score = 156 bits (394), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 77/184 (41%), Positives = 116/184 (63%), Gaps = 4/184 (2%) Query: 33 KDIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQA 92 KDIA+IG++ +P A N ++W + G IR+ P R +D + + ++ ++++ Sbjct: 670 KDIAIIGMSLNVPGASNKSDFWHLLENGEHGIREYPAPRVKDAIDYLRSI---KSERNEK 726 Query: 93 AYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTG 152 + GYLD ID FD +F ++PK AK MDPNQRLFLQ A+EDAGY + G+ G Sbjct: 727 QFVRGGYLDEIDRFDYSFFGLAPKTAKFMDPNQRLFLQSAWHAIEDAGYAGDTISGSQLG 786 Query: 153 VFLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACSS 212 V++GYS G +Y+ LLS E + GN+ S++ +R+AY+L++ GP++ VDTACSS Sbjct: 787 VYVGYSK-VGYDYERLLSANYPEELHHYIVGNLPSVLASRIAYFLNLKGPAVTVDTACSS 845 Query: 213 SLVA 216 SLVA Sbjct: 846 SLVA 849 >gb|AAS47562.1| mixed type I polyketide synthase - peptide synthetase [symbiont bacterium of Paederus fuscipes] Length = 6266 Score = 139 bits (350), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 72/182 (39%), Positives = 113/182 (62%), Gaps = 7/182 (3%) Query: 35 IAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQAAY 94 +A+IG++G+ P A + + +W+N+ G+D I +IP R D+ + K+ + D Sbjct: 1763 VAIIGMSGRFPMAEDIQAFWSNLLEGKDCISEIPEDR-WDWRAIYGDPTKEANKSD---V 1818 Query: 95 AESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTGVF 154 G++D + FD +F ISP+EA+LMDP QRL +Q +A+EDAGY L G+ T +F Sbjct: 1819 KWGGFIDGVAHFDARFFGISPREAELMDPQQRLLMQYVWKAVEDAGYAPASLSGSRTAIF 1878 Query: 155 LGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACSSSL 214 +G +S GE L++ E + + TG + S+ P R++Y+L++HGPS V+TACSSSL Sbjct: 1879 VGTASSGYGE---LMAQEGLAIESYSSTGVVGSVGPNRMSYFLNLHGPSEPVETACSSSL 1935 Query: 215 VA 216 VA Sbjct: 1936 VA 1937 Score = 119 bits (298), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 74/194 (38%), Positives = 103/194 (53%), Gaps = 19/194 (9%) Query: 34 DIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSR---KQDYEEVMSALLKKDCQKD 90 DIAVIG++G+ P A++ E+W N+ G+D + ++P R + Y E S C+ Sbjct: 2971 DIAVIGISGRYPMANDLDEFWLNLREGKDCVSEVPSQRWNWRDHYSEEHSRAGGHFCKW- 3029 Query: 91 QAAYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGY--------G 142 G++D ID FD +F+ISP A+ MDP +RLFL+ AMEDAGY Sbjct: 3030 ------GGFIDDIDKFDPLFFNISPSAAEYMDPQERLFLEHAWMAMEDAGYRREDLRKLA 3083 Query: 143 KGKLGGTPTGVFLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGP 202 +G G Y+ EY+ LL E+A A N + + R++Y LD+HGP Sbjct: 3084 RGSAAEDLPGQVGVYAGVMYSEYQ-LLGIEAARQGKGATVANFHASVANRVSYVLDLHGP 3142 Query: 203 SMLVDTACSSSLVA 216 SM VDT CSSSL A Sbjct: 3143 SMTVDTMCSSSLTA 3156 Score = 110 bits (275), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 67/182 (36%), Positives = 100/182 (54%), Gaps = 9/182 (4%) Query: 35 IAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQAAY 94 IA+I + G P + + E+ ++ G D I +IP R D+ EV K+ + + Y Sbjct: 223 IAIIAMDGVFPQSADLLEFERHLRQGDDCISEIPADR-WDWREVYGD--PKEGEFTRVKY 279 Query: 95 AESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPTGVF 154 G+ ID FD +F +SP+EA+LMDP R F+QC +E AGY L G+ G+F Sbjct: 280 G--GFAPDIDKFDPLFFGMSPREAQLMDPQHRQFIQCVWRLIESAGYAPKALSGSKVGLF 337 Query: 155 LGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACSSSL 214 +G + +Y HL+ A + L +T P RL++ L++HGPS ++DTACSSS Sbjct: 338 IGINLQ---DYAHLVDRADA-MDALHLTSLGHMFCPNRLSFLLNLHGPSQVIDTACSSSS 393 Query: 215 VA 216 VA Sbjct: 394 VA 395 Score = 105 bits (262), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 64/188 (34%), Positives = 103/188 (54%), Gaps = 15/188 (7%) Query: 33 KDIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDYEEVMSALLKKDCQKDQA 92 +DIA+IG++G+ P A + +W N++ G D I +IP +R + E + K Sbjct: 4180 RDIAIIGMSGRFPFAPDLEAFWENLSQGCDCITEIPPTRWKHQE-----YFDPEKGKPGK 4234 Query: 93 AYAE-SGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGY---GKGKLGG 148 Y + G+L+ ID FD +F I P +A+++DP +RLFL+ +E +GY ++ Sbjct: 4235 TYCKWGGFLESIDQFDPLFFKIPPAQAEVLDPQERLFLETVWNLLESSGYLGETLQRIAQ 4294 Query: 149 TPTGVFLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDT 208 + GVF+G S ++ L+ ES +T + S I R++Y+ D GPS+ VDT Sbjct: 4295 SRVGVFVGSMSQQYHAFQADLTRES------LVTMSSHSSIANRVSYFFDFQGPSVAVDT 4348 Query: 209 ACSSSLVA 216 CSS+LVA Sbjct: 4349 MCSSALVA 4356 >ref|NP_940497.1| Putative polyketide synthase [Corynebacterium diphtheriae] emb|CAE50717.1| Putative polyketide synthase [Corynebacterium diphtheriae] Length = 1586 Score = 135 bits (340), Expect = 9e-31, Method: Composition-based stats. Identities = 77/185 (41%), Positives = 105/185 (56%), Gaps = 12/185 (6%) Query: 34 DIAVIGLAGKLPDAHNAREYWTNITLGRDSIRQIPLSRKQDY--EEVMSALLKKDCQKDQ 91 DIA++G+A + P AH+ + W+ + GRD I +P R +Y +EV S +K Sbjct: 105 DIAIVGMAARYPGAHDIDQMWSMLIEGRDGITDLPEGRWSEYAADEVTS-------EKIA 157 Query: 92 AAYAESGYLDRIDGFDCEYFSISPKEAKLMDPNQRLFLQCCVEAMEDAGYGKGKLGGTPT 151 GYLD I FD E+F +SP EA +DP QR+ L+ EA+E A L G P Sbjct: 158 NTDLRGGYLDDIAFFDAEFFGLSPLEAVNIDPQQRIMLELTWEALEHAHIPASSLKGKPV 217 Query: 152 GVFLGYSSDFGGEYKHLLSGESAELADLAMTGNIKSIIPARLAYWLDVHGPSMLVDTACS 211 GVF G S++ +Y L+ + AE AMTGN SI+ R++Y D GPS+ VDTACS Sbjct: 218 GVFFGSSNN---DYGMLIGADPAESHPYAMTGNASSIVANRVSYAFDFRGPSVSVDTACS 274 Query: 212 SSLVA 216 SSLV+ Sbjct: 275 SSLVS 279 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 335,507,907 Number of extensions: 12902507 Number of successful extensions: 30917 Number of sequences better than 1.0e-05: 770 Number of HSP's gapped: 29499 Number of HSP's successfully gapped: 1065 Length of query: 247 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 125 Effective length of database: 425,995,500 Effective search space: 53249437500 Effective search space used: 53249437500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 124 (52.4 bits)