BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0489.0008.p (430 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAB69698.1| iturin A synthetase A [Bacillus subtilis] 348 1e-94 emb|CAE11250.1| BmyA protein [Bacillus amyloliquefaciens] 348 2e-94 pir||T44806 mycosubtilin synthetase chain mycA [imported] - Baci... 342 8e-93 ref|ZP_00074380.1| COG3321: Polyketide synthase modules and rela... 286 6e-76 gb|AAN32979.1| BarE [Lyngbya majuscula] 286 9e-76 >dbj|BAB69698.1| iturin A synthetase A [Bacillus subtilis] Length = 3982 Score = 348 bits (893), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 179/398 (44%), Positives = 246/398 (61%), Gaps = 17/398 (4%) Query: 12 GIGIVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQDGQS 71 G+ + SS T+TF +++DGTG GEGA VLLK + A++DGDHIY +IKGSA+NQDG + Sbjct: 879 GLDMESSDGYTKTFSKDSDGTGTGEGAAAVLLKPLQDAVRDGDHIYGVIKGSAMNQDGTT 938 Query: 72 IGITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDRYTRK 131 GITAPN AQ E+I AWKDA + PET+S IEAHGT T+LGDPVE +G+ +AF++YT K Sbjct: 939 AGITAPNPAAQTEVIETAWKDAGIAPETLSFIEAHGTGTKLGDPVEFNGLCKAFEKYTAK 998 Query: 132 KQFCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSPVYVN 191 KQFCAIGS+K+NIGHL AAGI GL+K +L L H+ PP HF PNP I F SP YVN Sbjct: 999 KQFCAIGSVKSNIGHLFEAAGIVGLIKSVLMLNHKKNPPLAHFNEPNPLIHFHSSPFYVN 1058 Query: 192 DAVRTWESGEHPRRCGINSFGLSGTNCHMILEQY---KETAAVERTGNLSADRWNMLTLS 248 + SG+ P R G++SFG SGTN H++LE+Y E A + G ++ LS Sbjct: 1059 QEAAEFTSGDEPLRGGVSSFGFSGTNAHVVLEEYISQSEYAPEDEHGP------HLFVLS 1112 Query: 249 AKNSDVLRRLVSEYHQFINQSESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEKLQS 308 A L L +Y Q+++ L ++CYTA+TGR H H A++ + +L +KL Sbjct: 1113 AHTEKSLYELAQQYRQYVSDDSQASLKSICYTASTGRAHLDHGIAMIVSGKQELSDKLTR 1172 Query: 309 LKQAGTGKISMEHVYVRPGHEQANAKIPGSSPDVQ---GIVQEMRAAVDQHRGSLLQQLA 365 L Q G ++ VY+ G++ +P ++ + + R R + L + A Sbjct: 1173 LIQ---GDRNLPGVYI--GYKNMKEMLPAHKEELNQQAAALIKQRLRTQDERITWLHRAA 1227 Query: 366 QLYVQGEAIPWQSLYADGRYQLISLPAYPFQETRCWIE 403 +L+VQG I W++LY+ Q LP YPF+ +RCW E Sbjct: 1228 ELFVQGAVIDWRALYSGETVQKTPLPLYPFERSRCWAE 1265 >emb|CAE11250.1| BmyA protein [Bacillus amyloliquefaciens] Length = 3982 Score = 348 bits (893), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 179/398 (44%), Positives = 246/398 (61%), Gaps = 17/398 (4%) Query: 12 GIGIVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQDGQS 71 G+ + SS T+TF +++DGTG GEGA VLLK + A++DGDHIY +IKGSA+NQDG + Sbjct: 879 GLDMESSDGYTKTFSKDSDGTGTGEGAAAVLLKPLQDAVRDGDHIYGVIKGSAMNQDGTT 938 Query: 72 IGITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDRYTRK 131 GITAPN AQ E+I AWKDA + PET+S IEAHGT T+LGDPVE +G+ +AF++YT K Sbjct: 939 AGITAPNPAAQTEVIETAWKDAGIAPETLSFIEAHGTGTKLGDPVEFNGLCKAFEKYTAK 998 Query: 132 KQFCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSPVYVN 191 KQFCAIGS+K+NIGHL AAGI GL+K +L L H+ PP HF PNP I F SP YVN Sbjct: 999 KQFCAIGSVKSNIGHLFEAAGIVGLIKSVLMLNHKKNPPLAHFNEPNPLIHFHSSPFYVN 1058 Query: 192 DAVRTWESGEHPRRCGINSFGLSGTNCHMILEQY---KETAAVERTGNLSADRWNMLTLS 248 + SG+ P R G++SFG SGTN H++LE+Y E A + G ++ LS Sbjct: 1059 QEAAEFTSGDEPLRGGVSSFGFSGTNAHVVLEEYISQSEYAPEDEHGP------HLFVLS 1112 Query: 249 AKNSDVLRRLVSEYHQFINQSESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEKLQS 308 A L L +Y Q+++ L ++CYTA+TGR H H A++ + +L +KL Sbjct: 1113 AHTEKSLYELAQQYRQYVSDDSQASLKSICYTASTGRAHLDHGIAMIVSGKQELSDKLTR 1172 Query: 309 LKQAGTGKISMEHVYVRPGHEQANAKIPGSSPDVQ---GIVQEMRAAVDQHRGSLLQQLA 365 L Q G ++ VY+ G++ +P ++ + + R R + L + A Sbjct: 1173 LIQ---GDRNLPGVYI--GYKNMKEMLPAHKEELNQQAAALIKQRLRTQDERITWLNRAA 1227 Query: 366 QLYVQGEAIPWQSLYADGRYQLISLPAYPFQETRCWIE 403 +L+VQG I W++LY+ Q LP YPF+ +RCW E Sbjct: 1228 ELFVQGAVIDWRALYSGETVQKTPLPLYPFERSRCWAE 1265 >pir||T44806 mycosubtilin synthetase chain mycA [imported] - Bacillus subtilis gb|AAF08795.1| MycA [Bacillus subtilis] Length = 3971 Score = 342 bits (878), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 178/393 (45%), Positives = 243/393 (61%), Gaps = 7/393 (1%) Query: 12 GIGIVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQDGQS 71 G+ + SS T+TF +++DGTG GEG VLLK + AI+DGDHIY +IKGSA+NQDG + Sbjct: 880 GLDMESSDGLTKTFSKDSDGTGSGEGVAAVLLKPLQAAIRDGDHIYGVIKGSAINQDGTT 939 Query: 72 IGITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDRYTRK 131 +GITAP+ AQ E+I AWKDA + PET+S IEAHGT T+LGDPVE +G+ +AF++ T K Sbjct: 940 VGITAPSPAAQTEVIEMAWKDAGIAPETLSFIEAHGTGTKLGDPVEFNGLCKAFEKVTEK 999 Query: 132 KQFCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSPVYVN 191 KQFCAIGS+K NIGHL AAGI GL+K L L H+ +PP HF PNP I F SP YVN Sbjct: 1000 KQFCAIGSVKANIGHLFEAAGIVGLIKSALMLNHKKIPPLAHFNKPNPLIPFHSSPFYVN 1059 Query: 192 DAVRTWESGEHPRRCGINSFGLSGTNCHMILEQYK-ETAAVERTGNLSADRWNMLTLSAK 250 V + + P R GI+SFG SGTN H++LE+Y E+ GN ++ LSA Sbjct: 1060 QEVMDFTPEDRPLRGGISSFGFSGTNAHVVLEEYTPESEYAPEDGNDP----HLFVLSAH 1115 Query: 251 NSDVLRRLVSEYHQFINQSESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEKLQSLK 310 L L +Y Q+I+ L ++CYTA+TGR H + A++ + +L++KL SL Sbjct: 1116 TEASLYELTHQYRQYISDDSQSSLRSICYTASTGRAHLDYCLAMIVSSNQELIDKLTSLI 1175 Query: 311 QAGTGKISMEHVYVRPGHEQANAKIPGSSPDVQGIVQEMRAAVDQHRGSLLQQLAQLYVQ 370 Q G + H + E A+ S + ++Q R R + L ++A+LYVQ Sbjct: 1176 Q-GERNLPQVHFGYKNIKEMQPAEKDNLSKQISDLMQH-RPCTKDERITWLNRIAELYVQ 1233 Query: 371 GEAIPWQSLYADGRYQLISLPAYPFQETRCWIE 403 I W+++Y++ Q LP YPF+ RCW+E Sbjct: 1234 RAVIDWRAVYSNEVVQKTPLPLYPFERNRCWVE 1266 >ref|ZP_00074380.1| COG3321: Polyketide synthase modules and related proteins [Trichodesmium erythraeum IMS101] Length = 1909 Score = 286 bits (732), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 142/293 (48%), Positives = 191/293 (65%), Gaps = 12/293 (4%) Query: 23 RTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQDGQ-SIGITAPNSKA 81 R FD + GT G G GVVLLK + AI DGD I+A+IKGSA+N DG +G TAP+ Sbjct: 237 RAFDARSSGTVFGNGVGVVLLKPLQDAIADGDCIHAVIKGSAINNDGSLKLGYTAPSVDG 296 Query: 82 QEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDRYTRKKQFCAIGSIK 141 Q +I A A V PETI+ IEAHGT T LGDP+EI+ +T+AF +T KKQFCAIGS+K Sbjct: 297 QAAVISEAQAVASVTPETITYIEAHGTGTELGDPIEIEALTKAFSEHTDKKQFCAIGSLK 356 Query: 142 TNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSPVYVNDAVRTWESGE 201 TN+GH++ AAGI L+K +L L+H +PP++H++ PNP I+F DSP YVN + W+ Sbjct: 357 TNVGHMNTAAGIGALIKTVLTLKHNLIPPSLHYEKPNPQINFSDSPFYVNSTLSEWKRNG 416 Query: 202 HPRRCGINSFGLSGTNCHMILE----QYKETAAVERTGNLSADRWNMLTLSAKNSDVLRR 257 P R G++SFG+ GTN H++LE Q KE ++R ++ LTLSAK L Sbjct: 417 TPLRAGVSSFGIGGTNSHVVLEEAPSQVKEEDDLQRPVHI-------LTLSAKTPTALAD 469 Query: 258 LVSEYHQFINQSESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEKLQSLK 310 LV YH ++ + +DL ++CYTANTGR H H+ A+V + +L EKL+ K Sbjct: 470 LVDSYHHYLETNLDLDLGDVCYTANTGRVHFNHRLAVVAEKQTELTEKLRQFK 522 >gb|AAN32979.1| BarE [Lyngbya majuscula] Length = 1706 Score = 286 bits (731), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 140/300 (46%), Positives = 195/300 (65%), Gaps = 5/300 (1%) Query: 15 IVSSGFRTRTFDQNADGTGLGEGAGVVLLKDARQAIQDGDHIYALIKGSAVNQDGQS-IG 73 I S R FD A+GT G G G+V LK +A+QDGDHIYA+IKGSA+N DG + + Sbjct: 922 IASPDGHCRAFDAQAEGTIFGNGVGLVALKRLEEALQDGDHIYAVIKGSAINNDGSNKVA 981 Query: 74 ITAPNSKAQEELIIRAWKDAEVHPETISLIEAHGTATRLGDPVEIDGITRAFDRYTRKKQ 133 TAPN + Q ++ A A ++PETI+ +EAHGT T LGDP+E+ +++AF T++KQ Sbjct: 982 YTAPNGEGQTTVVAEAIATANINPETITYVEAHGTGTLLGDPIELGALSQAFQAKTQRKQ 1041 Query: 134 FCAIGSIKTNIGHLDHAAGISGLLKCILALQHETLPPTIHFQSPNPNISFPDSPVYVNDA 193 FCA+GS+KTN+GHL +GI GL+K +LAL H+ +PP++HFQ+PNP I F +SP +VN Sbjct: 1042 FCALGSVKTNVGHLQITSGIVGLIKTVLALYHKQIPPSLHFQTPNPKIDFANSPFFVNTM 1101 Query: 194 VRTWESGEHPRRCGINSFGLSGTNCHMILEQYKETAAV----ERTGNLSADRWNMLTLSA 249 + WE HPRR G+NS G GTN H+ILE+ E A E L ++LTLSA Sbjct: 1102 LSEWERNGHPRRAGVNSLGFGGTNAHVILEEAPENAQPKTRNEDGAKLEDRSHHLLTLSA 1161 Query: 250 KNSDVLRRLVSEYHQFINQSESVDLDNLCYTANTGRDHHVHKAAIVFRNRMDLLEKLQSL 309 K + L+ LV Y ++++ + V+L ++CYTA GR H H+ AIV + DL KL ++ Sbjct: 1162 KTAPALQALVKRYQRYLSDNTEVELGDICYTACVGRAHFEHRLAIVSDSIPDLRCKLANV 1221 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 645,672,668 Number of extensions: 26972132 Number of successful extensions: 66610 Number of sequences better than 1.0e-05: 1792 Number of HSP's gapped: 63704 Number of HSP's successfully gapped: 2094 Length of query: 430 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 302 Effective length of database: 409,704,906 Effective search space: 123730881612 Effective search space used: 123730881612 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)