BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0488.0001.p (390 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_347542.1| Alanyl-tRNA synthetase related protein [Clostri... 263 5e-69 ref|NP_657529.1| tRNA-synt_2c, tRNA synthetases class II (A) [Ba... 246 7e-64 ref|NP_845947.1| alanyl-tRNA synthetase family protein [Bacillus... 244 2e-63 ref|NP_979940.1| alanyl-tRNA synthetase family protein [Bacillus... 243 6e-63 ref|NP_622586.1| predicted metal-dependent hydrolases related to... 229 9e-59 >ref|NP_347542.1| Alanyl-tRNA synthetase related protein [Clostridium acetobutylicum] pir||G97011 alanyl-tRNA synthetase related protein [imported] - Clostridium acetobutylicum gb|AAK78882.1| Alanyl-tRNA synthetase related protein [Clostridium acetobutylicum] Length = 387 Score = 263 bits (673), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 133/387 (34%), Positives = 223/387 (57%), Gaps = 3/387 (0%) Query: 1 MTKKLYYESAYLKDWHTSVSQVVEREDGLYLILEETAFYPHGGGQPCDAGTIGGIPVLDV 60 MT+KL+Y+ Y+ + + V+E + ++L+ET+FYP GGGQPCD GTI IPV V Sbjct: 1 MTRKLFYDDPYICESEGKIVDVLENDGKYEIVLDETSFYPEGGGQPCDLGTINNIPVEYV 60 Query: 61 ILEENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFHLG 120 + + + H ++ P G+ C++D+ RR D++QQH+G+HLLSA F +L+ + FH+G Sbjct: 61 YEKGDTIYHVMKERPEGNTAKCKVDFLRRQDNIQQHTGEHLLSAAFFKLYNGVNYGFHMG 120 Query: 121 NDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTENI 180 DY TID+++ E+T D + E E N + + +YF++ E LPL K K I Sbjct: 121 EDYVTIDIDMEEVTNDMIKKIEAEANAYIYANVETKTYFMSKPEAEKLPLRKAIKAEGKI 180 Query: 181 RIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQRIL 240 RIV++ V+++AC GTHV TG +G+IK+LR EK KG R+ FKCG RA DE + I+ Sbjct: 181 RIVQLGDVDYSACCGTHVVRTGEVGIIKVLRTEKYKGMTRVYFKCGRRAFDEYSKEHNII 240 Query: 241 SALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELLSGLEPETRVVAH 300 + L + +D IL+++ + E+ + + ++N+ + E + + H Sbjct: 241 TKLQKNLSIDEDNILNKV-QTTMEEISNLKKKLLEYKKNEAKLEVEKIKEKNNNGVIFEH 299 Query: 301 IFADKSLKDLQSLATKLTANTELPVLLLTAMENKAVLACSGSAGLSCGAFFKAHLGEFQG 360 + D L+ + + + + ++L + ++N+ + A +G + CG FK ++ E+ G Sbjct: 300 -YEDGDFSFLEEIYDGI-KDEKAVIILTSGVDNRILFAQNGIDDIECGKIFKENIKEYNG 357 Query: 361 KGGGSDKMAQAGFPAWDEVLKFYEFAK 387 +GGG+ K AQA F D++ KF +F + Sbjct: 358 RGGGNAKRAQAAFSNKDDLNKFAKFLR 384 >ref|NP_657529.1| tRNA-synt_2c, tRNA synthetases class II (A) [Bacillus anthracis A2012] Length = 400 Score = 246 bits (628), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 142/385 (36%), Positives = 215/385 (55%), Gaps = 14/385 (3%) Query: 1 MTKKLYYESAYLKDWHTSV-SQVVEREDGLYLILEETAFYPHGGGQPCDAGTIGGIPVLD 59 M +KLYY AY +D+ T V Q +++ LY+IL ET FYP GGGQP D GT+ GI VL Sbjct: 1 MEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLG 60 Query: 60 VILEENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFHL 119 V + + H + +V +++W RRFDHMQQH+ QH+LSA F + F T+ FHL Sbjct: 61 VEEVDEEIRHFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHL 120 Query: 120 GNDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTEN 179 G + TIDLE L + + A Q N+ V + I+ ++ EE +LPL K P +TEN Sbjct: 121 GKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKWMNLEEAKTLPLRKEPTMTEN 180 Query: 180 IRIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQRI 239 IR+V IE ++N CGGTH TG +G I++L E+ KG IR+TF G R L Q+I Sbjct: 181 IRVVMIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWRTLKLMGQQQQI 240 Query: 240 LSALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELLSGLEPETRV-- 297 + +S + N+ + +I ++ + QK+ + M + E+ A ELL +P T++ Sbjct: 241 MKDVSKQLNSSEADIPAKVAQLLTSQKENKKAMQTMTEKLLYTEANELLQ--QP-TKIHA 297 Query: 298 ---VAHIFADKSLKDLQSLATKLTANTELPVLLLTAMEN----KAVLACSGSAGLSCGAF 350 ++ +F ++S++++ L+ +T E + +EN + +LAC + L A Sbjct: 298 GILISKVFTNRSMQEIAKLSAIITEQQEHAITYF-VIENEDKLQCILACGKTVTLDMNAL 356 Query: 351 FKAHLGEFQGKGGGSDKMAQAGFPA 375 K L +GKGGG+ K A+ G A Sbjct: 357 LKEALPTIEGKGGGNKKSARGGGKA 381 >ref|NP_845947.1| alanyl-tRNA synthetase family protein [Bacillus anthracis str. Ames] gb|AAP27433.1| alanyl-tRNA synthetase family protein [Bacillus anthracis str. Ames] Length = 411 Score = 244 bits (624), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 141/385 (36%), Positives = 215/385 (55%), Gaps = 14/385 (3%) Query: 1 MTKKLYYESAYLKDWHTSV-SQVVEREDGLYLILEETAFYPHGGGQPCDAGTIGGIPVLD 59 + +KLYY AY +D+ T V Q +++ LY+IL ET FYP GGGQP D GT+ GI VL Sbjct: 12 LEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVILNETVFYPTGGGQPHDTGTLNGIAVLG 71 Query: 60 VILEENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFHL 119 V + + H + +V +++W RRFDHMQQH+ QH+LSA F + F T+ FHL Sbjct: 72 VEEVDEEIRHFIAEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWDYFNIPTIGFHL 131 Query: 120 GNDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTEN 179 G + TIDLE L + + A Q N+ V + I+ ++ EE +LPL K P +TEN Sbjct: 132 GKETVTIDLETENLHAETVEKAVQIANKIVFENHPILIKWMNLEEAKTLPLRKEPTMTEN 191 Query: 180 IRIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQRI 239 IR+V IE ++N CGGTH TG +G I++L E+ KG IR+TF G R L Q+I Sbjct: 192 IRVVMIENFDYNGCGGTHPKRTGEVGPIQVLGWERNKGGIRLTFIAGWRTLKLMGQQQQI 251 Query: 240 LSALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELLSGLEPETRV-- 297 + +S + N+ + +I ++ + QK+ + M + E+ A ELL +P T++ Sbjct: 252 MKDVSKQLNSSEADIPAKVAQLLTSQKENKKAMQTMTEKLLYTEANELLQ--QP-TKIHA 308 Query: 298 ---VAHIFADKSLKDLQSLATKLTANTELPVLLLTAMEN----KAVLACSGSAGLSCGAF 350 ++ +F ++S++++ L+ +T E + +EN + +LAC + L A Sbjct: 309 GILISKVFTNRSMQEIAKLSAIITEQQEHAITYF-VIENEDKLQCILACGKTVTLDMNAL 367 Query: 351 FKAHLGEFQGKGGGSDKMAQAGFPA 375 K L +GKGGG+ K A+ G A Sbjct: 368 LKEALPTIEGKGGGNKKSARGGGKA 392 >ref|NP_979940.1| alanyl-tRNA synthetase family protein [Bacillus cereus ATCC 10987] gb|AAS42548.1| alanyl-tRNA synthetase family protein [Bacillus cereus ATCC 10987] Length = 400 Score = 243 bits (620), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 140/387 (36%), Positives = 213/387 (55%), Gaps = 18/387 (4%) Query: 1 MTKKLYYESAYLKDWHTSV-SQVVEREDGLYLILEETAFYPHGGGQPCDAGTIGGIPVLD 59 M +KLYY AY +D+ T V Q +++ LY++L ETAFYP GGGQP D GT+ GI VLD Sbjct: 1 MEQKLYYTDAYKQDFTTKVIKQDYDKDGNLYVVLNETAFYPTGGGQPHDTGTLNGIAVLD 60 Query: 60 VILEENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFHL 119 V + + H + +V +++W RRFDHMQQH+ QH+LSA F + F T+ FHL Sbjct: 61 VEEVDEEIRHFITEQLHTEEVEGKINWERRFDHMQQHAAQHILSAAFWDHFNIPTIGFHL 120 Query: 120 GNDYATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTEN 179 G + TIDLE L + + A Q N V + I ++ EE +LPL K P + EN Sbjct: 121 GRETVTIDLETENLHTETVEKAVQIANNIVFENHPIRIEWMNLEEAKTLPLRKEPTMIEN 180 Query: 180 IRIVEIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQRI 239 IR+V IE ++N CGGTH TG +G I++L E+ KG IR+TF G R L Q+I Sbjct: 181 IRVVMIENFDYNGCGGTHPKRTGEVGPIQVLSWERNKGGIRLTFIAGWRTLKLVGQQQQI 240 Query: 240 LSALSAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQNDIYMAKELL-------SGLE 292 + +S + N+ + +I ++ + QK+ + + + E+ A ELL +G+ Sbjct: 241 MKDVSKQLNSSESDISAKVAQLLTSQKENEKTIQTMTEKLLYAEANELLQHPTEIHAGI- 299 Query: 293 PETRVVAHIFADKSLKDLQSLATKLTANTELPVLLLTAMEN----KAVLACSGSAGLSCG 348 +++ +F ++S++++ L+ +T E + +EN + +LAC + L Sbjct: 300 ----LISKMFTNRSMQEVSKLSAIITEQQEHAITYF-VIENDDKLQCILACGKTVTLDMN 354 Query: 349 AFFKAHLGEFQGKGGGSDKMAQAGFPA 375 A K L +GKGGG+ K A+ G A Sbjct: 355 ALLKKALPTIEGKGGGNKKSARGGGKA 381 >ref|NP_622586.1| predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain [Thermoanaerobacter tengcongensis] gb|AAM24190.1| predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain [Thermoanaerobacter tengcongensis] Length = 418 Score = 229 bits (584), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 135/393 (34%), Positives = 218/393 (55%), Gaps = 18/393 (4%) Query: 4 KLYYESAYLKDWHTSVSQVVEREDGLYLILEETAFYPHGGGQPCDAGTIGGIPVLDVILE 63 KL+YE +Y+K++ + + E++ ++L+ T FYP GGGQP D G IG V+ + Sbjct: 22 KLFYEDSYVKEFEAVILKREEKDGEKTVVLDRTYFYPEGGGQPSDTGKIGSKSVIKAYEK 81 Query: 64 ENVVLHKVESLPAGSQVSCQLDWNRRFDHMQQHSGQHLLSAVFRELFQAMTLSFHLGNDY 123 + V H VE P G +V C +DW+RRFDHMQQH+GQHLLS+V + + A T SF +G + Sbjct: 82 KGEVYHVVEDFPEGERVHCVIDWDRRFDHMQQHTGQHLLSSVLLKKYGADTESFSIGENS 141 Query: 124 ATIDLELRELTPDQLAAAEQEVNRQVVLDRNIVSYFVTPEEMASLPLVKLPKVTENIRIV 183 + I + T +++ E+EVN + + + SYF + ++ LPL K P+V ENIRIV Sbjct: 142 SHITVTKEFFTEEEIHEVEKEVNGLIYKNLAVKSYFADEKLISKLPLRKKPQVKENIRIV 201 Query: 184 EIEGVEHNACGGTHVSSTGAIGMIKLLRAEKQKGNIRITFKCGGRALDEANDNQRILSAL 243 EIEG++++ CGGTHV +TG IG++K+ + E+ R+ F CG RAL++ +++ L Sbjct: 202 EIEGIDYSPCGGTHVKNTGEIGILKIKKWERTNKGARLEFVCGFRALEDYQKKNSLVNFL 261 Query: 244 SAKFNTGKDEILDRIGKWEAEQKQLQAEMAVLREQ------NDIYMAKELLSGLEPETRV 297 + ++ I + + K + ++ + E+ L+E+ ++ EL G+ + Sbjct: 262 IQVLSEKEEGIAEHLQKLLEDYREAKREVNFLKEKILKEEAENLVKKSELYGGI----HI 317 Query: 298 VAHIFADKSLKDLQSLATKLTANTELPVLLLTAMENKA--VLACSGSAGLSCGAFFKAHL 355 V ++ + +DL+ L +TA ++ +L + A V+A S + FFK L Sbjct: 318 VYSLYEGRDPEDLKMLGKFVTAESKSVAILGNKRGDVADIVVARSYDVDIVVNGFFKEVL 377 Query: 356 GEFQGKGGGSDKMAQAGFP------AWDEVLKF 382 F GKGGGS + Q G A D+ LKF Sbjct: 378 RTFDGKGGGSAQFCQGGGSPSRLELAMDKALKF 410 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 562,044,083 Number of extensions: 22943208 Number of successful extensions: 63917 Number of sequences better than 1.0e-05: 515 Number of HSP's gapped: 63167 Number of HSP's successfully gapped: 518 Length of query: 390 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 262 Effective length of database: 409,704,906 Effective search space: 107342685372 Effective search space used: 107342685372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)