BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0484.0001.p (190 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_693794.1| 4-hydroxyphenylacetate-3-hydroxylase [Oceanobac... 115 4e-25 ref|NP_244703.1| 4-hydroxyphenylacetate-3-hydroxylase [Bacillus ... 115 4e-25 ref|NP_978461.1| 4-hydroxyphenylacetate-3-hydroxylase [Bacillus ... 113 2e-24 gb|EAI23481.1| unknown [environmental sequence] 107 2e-22 gb|EAH89738.1| unknown [environmental sequence] 103 2e-21 >ref|NP_693794.1| 4-hydroxyphenylacetate-3-hydroxylase [Oceanobacillus iheyensis HTE831] dbj|BAC14828.1| 4-hydroxyphenylacetate-3-hydroxylase [Oceanobacillus iheyensis HTE831] Length = 498 Score = 115 bits (289), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 54/159 (33%), Positives = 98/159 (61%), Gaps = 3/159 (1%) Query: 6 MAVKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQM 65 M K G++Y +++ + N++ G+ ++ +++HPAF G +++ A++YD+Q++K E+M Sbjct: 1 MPAKTGQQYIDKLKKANNNVYIHGERVED-VTTHPAFKGAVQSMAKLYDLQYEKP--EKM 57 Query: 66 TFQSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAA 125 + S T G++ +F+AP + E+L+ RR + W G +GRSPDY+N+ +M++ A Sbjct: 58 LYTSPTTGDKVSKTFMAPETIEELIERREAIMEWQGITKGLMGRSPDYLNSEVMTMEKAY 117 Query: 126 HLLQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 + N + +N KKY EY R DI+L+H I P +R Sbjct: 118 DYYGKSNSLFAENAKKYAEYARENDISLTHTLIHPQVNR 156 >ref|NP_244703.1| 4-hydroxyphenylacetate-3-hydroxylase [Bacillus halodurans] pir||D84129 4-hydroxyphenylacetate-3-hydroxylase BH3836 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07555.1| 4-hydroxyphenylacetate-3-hydroxylase [Bacillus halodurans] Length = 479 Score = 115 bits (289), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 57/159 (35%), Positives = 89/159 (55%), Gaps = 3/159 (1%) Query: 6 MAVKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQM 65 MA+ G +Y ERIN+ Q +W G+ + +S HPAF G+MK++A +YD+ H + + Sbjct: 1 MAIIKGSDYLERINKLQNEVWIGGEKVTGAISEHPAFKGIMKSKAALYDLYHHSDKKDLL 60 Query: 66 TFQSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAA 125 T E + S+ P++KE L RR +L + G +GRSPDY+NT +MSL + Sbjct: 61 TCWDEKTNEYHSFSYSQPKTKEQLRNRRQATQLLARESGGTMGRSPDYINTAIMSLGSGG 120 Query: 126 HLLQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 + + Y DN +K +E R +D + +H FI P +R Sbjct: 121 AGMPK---PYGDNARKIFEEAREKDFSFTHTFINPQVNR 156 >ref|NP_978461.1| 4-hydroxyphenylacetate-3-hydroxylase [Bacillus cereus ATCC 10987] gb|AAS41069.1| 4-hydroxyphenylacetate-3-hydroxylase [Bacillus cereus ATCC 10987] Length = 490 Score = 113 bits (283), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 51/159 (32%), Positives = 93/159 (58%), Gaps = 1/159 (0%) Query: 6 MAVKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQM 65 M ++ G++Y E + ++ IW G+ + T + + F + A +YDMQH ++ +++ Sbjct: 1 MGIRTGEQYIEGLRKRNPEIWIGGRKV-TDVVNEDVFREPILQIANLYDMQHHPEYQDKI 59 Query: 66 TFQSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAA 125 T GER +FL P++ EDL RR + E+W + G +GR+PD++N T+ S+ + + Sbjct: 60 THICEETGERVSNAFLVPKNYEDLKARRELFEVWAKATFGLMGRTPDFLNVTVTSMASNS 119 Query: 126 HLLQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 +++NPE+ N+K YY++ + D+ L+HA I P R Sbjct: 120 WFFEKFNPEWGANIKNYYKHIKDNDLFLTHAIINPQNDR 158 >gb|EAI23481.1| unknown [environmental sequence] Length = 223 Score = 107 bits (266), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 55/159 (34%), Positives = 87/159 (54%), Gaps = 2/159 (1%) Query: 6 MAVKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQM 65 M V+ +Y E + + +I+ +G+ IK ++ F G +T AE+ MQH+K E + Sbjct: 1 MPVRTAAQYMESLKDDR-SIYIEGERIKD-VTKDNRFVGAAETMAELLQMQHEKDLKEVL 58 Query: 66 TFQSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAA 125 T+ S T GE G+S + P+SKED+ R M++W++ G LGRSPDY N + + AA Sbjct: 59 TYNSPTSGESVGMSHIQPKSKEDIKARGDAMKVWMDATCGMLGRSPDYKNVMVSAWAAAA 118 Query: 126 HLLQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 + + N+K YYEY R D+ ++H + P R Sbjct: 119 KSFERETFDGSANIKNYYEYVRENDLMMTHVLVNPQVDR 157 >gb|EAH89738.1| unknown [environmental sequence] Length = 492 Score = 103 bits (257), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 49/157 (31%), Positives = 90/157 (57%), Gaps = 2/157 (1%) Query: 8 VKNGKEYTERINRQQINIWYQGKIIKTPLSSHPAFNGLMKTQAEMYDMQHQKKFMEQMTF 67 +KNG ++ + R ++ G+ + +++H AF +++ A +YD Q + + +E+MTF Sbjct: 2 IKNGTQHIASL-RDGRQVYLNGQPVGD-VTAHAAFRNSIRSYASLYDYQARDENVEKMTF 59 Query: 68 QSGTDGERYGLSFLAPRSKEDLVRRRIMMELWVEKHHGFLGRSPDYMNTTLMSLYTAAHL 127 S G R + P S ++LV RR +E W E H+GF+GRSPD++ + L ++ A + Sbjct: 60 VSPDSGHRVSRIWQLPTSYDELVERRAALEAWSELHYGFMGRSPDHVASCLSGMFMGADV 119 Query: 128 LQEYNPEYEDNLKKYYEYCRSQDITLSHAFIQPFASR 164 ++Y+P L+ YY Y R D+ L++ + P A++ Sbjct: 120 FEQYDPARAGALRDYYRYARDNDLFLTYVIVNPQANQ 156 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.134 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 273,709,021 Number of extensions: 10374585 Number of successful extensions: 23460 Number of sequences better than 1.0e-05: 50 Number of HSP's gapped: 23416 Number of HSP's successfully gapped: 50 Length of query: 190 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 71 Effective length of database: 434,140,797 Effective search space: 30823996587 Effective search space used: 30823996587 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 122 (51.6 bits)