BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0483.0004.p (213 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_863958.1| probable secreted glycosyl hydrolase [Pirellula... 77 3e-13 ref|NP_357362.1| AGR_L_3146p [Agrobacterium tumefaciens] >gi|179... 76 4e-13 ref|NP_387173.1| HYPOTHETICAL PROTEIN [Sinorhizobium meliloti] >... 69 7e-11 gb|EAJ56514.1| unknown [environmental sequence] 66 6e-10 gb|EAJ40229.1| unknown [environmental sequence] 65 1e-09 >ref|NP_863958.1| probable secreted glycosyl hydrolase [Pirellula sp. 1] emb|CAD71632.1| probable secreted glycosyl hydrolase [Pirellula sp.] Length = 1557 Score = 77.0 bits (188), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 48/171 (28%), Positives = 77/171 (45%), Gaps = 6/171 (3%) Query: 42 TEDYSLLSKDSLSEYKLVIAYTEFSDHKIPPEQSGGFLSYVASGGGLLVVHNGI-SLQRN 100 T+D S+LS + L++Y ++ Y + P Q+ L YV GGG + +H + N Sbjct: 127 TDDMSMLSLEKLNQYDALVLYANIDEIAKP--QADALLQYVEEGGGFVPLHCATYCFRNN 184 Query: 101 QELGVMLGGHFTHHPAYTSLQMTVPAT-GHPIMEGITDFVMEDEPYYFDMQPHFETTILA 159 E+ ++G F H T + T P H +M+G + F DE Y + T+L Sbjct: 185 SEIVALMGAQFQRH--GTGVFRTTPDKPNHEVMKGYSGFESWDETYVHHLHNEANRTVLE 242 Query: 160 EYPHGGAMRPAAWCHEFGQGRVVYLMPGHHLPSFSVEPFRKMIKRGGLWAA 210 P W + G+GRV Y GH +++ F +++RG W A Sbjct: 243 YRVDSNGREPWTWVRDQGKGRVFYTAWGHDSRTWTNPGFLNLVERGIRWVA 293 >ref|NP_357362.1| AGR_L_3146p [Agrobacterium tumefaciens] ref|NP_533742.1| conserved hypothetical protein [Agrobacterium tumefaciens str. C58 (U. Washington)] pir||A98328 hypothetical protein AGR_L_3146 [imported] - Agrobacterium tumefaciens (strain C58, Cereon) pir||AD2955 conserved hypothetical protein Atu3242 [imported] - Agrobacterium tumefaciens (strain C58, Dupont) gb|AAK90147.1| AGR_L_3146p [Agrobacterium tumefaciens str. C58 (Cereon)] gb|AAL44058.1| conserved hypothetical protein [Agrobacterium tumefaciens str. C58 (U. Washington)] Length = 213 Score = 76.3 bits (186), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 57/195 (29%), Positives = 88/195 (45%), Gaps = 16/195 (8%) Query: 28 VFEELLGEE-LQVQSTEDYSLLSKDSLSEYKLVIAYTEFSDHKIPPEQSGGFLSYVASGG 86 + +++L E+ +V + S+ + LV+ S KI E+ + V +G Sbjct: 22 IVKDILEEDGFKVYVEHSTEAFADPSVHDLSLVVPIVTMS--KIEKEEIKNLTAAVENGV 79 Query: 87 GLLVVHNGI--SLQRNQELGVMLGGHFTHHPAYT---SLQMTVPATGHPIMEGITDFVME 141 G+ H G S + E M+GG + HP ++ +T P P+MEGI+DF Sbjct: 80 GIAGFHGGAGDSFRECVEYQFMIGGQWVAHPGNIIDYTVNITRP--DDPLMEGISDFPYT 137 Query: 142 DEPYYFDMQPHFE----TTILAEYPH--GGAMRPAAWCHEFGQGRVVYLMPGHHLPSFSV 195 E YY + P E TT ++ G + P W ++G+GRV Y GH FSV Sbjct: 138 SEQYYMHVDPSNEVLATTTFTGDHAWWIDGVVMPVVWKRKYGKGRVFYSALGHQAKEFSV 197 Query: 196 EPFRKMIKRGGLWAA 210 + M +RG WAA Sbjct: 198 PQMKTMFRRGANWAA 212 >ref|NP_387173.1| HYPOTHETICAL PROTEIN [Sinorhizobium meliloti] emb|CAC47646.1| HYPOTHETICAL PROTEIN [Sinorhizobium meliloti] Length = 213 Score = 68.9 bits (167), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 50/168 (29%), Positives = 74/168 (44%), Gaps = 11/168 (6%) Query: 52 SLSEYKLVIAYTEFSDHKIPPEQSGGFLSYVASGGGLLVVHNGI--SLQRNQELGVMLGG 109 S+ + L++ S KI E+ + V G G+ H G + + + E ++GG Sbjct: 47 SIHDLSLIVPIMTMS--KIEKEEVKNLAAAVEGGVGIAGYHGGAGDAFRESVEYQFIIGG 104 Query: 110 HFTHHPA-YTSLQMTVPATGHPIMEGITDFVMEDEPYYFDMQPHFE----TTILAEYPHG 164 + HP ++ V P+MEGI+DF E YY + P E TT E+ Sbjct: 105 QWVAHPGNIIDYRVNVTRPDDPLMEGISDFPYTSEQYYMHVDPSNEVLATTTFSGEHASW 164 Query: 165 --GAMRPAAWCHEFGQGRVVYLMPGHHLPSFSVEPFRKMIKRGGLWAA 210 G + P W + G+GRV Y GH F V R + +RG WAA Sbjct: 165 IEGVVMPVVWKRKHGKGRVFYSSLGHVAKEFEVPEMRTIFRRGANWAA 212 >gb|EAJ56514.1| unknown [environmental sequence] Length = 237 Score = 65.9 bits (159), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 50/194 (25%), Positives = 85/194 (43%), Gaps = 23/194 (11%) Query: 35 EELQVQSTEDYSLLSK-------DSLSEYKLVIAYTEFSDHKIPPEQSGGFLSYVASGGG 87 E+ + DY +++ D L +Y L+I + I +Q + V +G G Sbjct: 48 EKWLISQNADYKIINGLDAYDNYDKLIKYDLIIQSVTMDE--ISKKQEENLVKAVLNGVG 105 Query: 88 LLVVHNGI--SLQRNQELGVMLGGHFTHHPAYTSLQMTVPATGHPIMEGITDFVMEDEPY 145 + H G+ S ++N M+GG F HP ++ V + G++DF + E Y Sbjct: 106 IAGAHGGLIDSFRKNTNYQFMIGGQFVAHPG-GMIKHKVKILDDELTIGLSDFDILSEQY 164 Query: 146 YFDMQPHFETTILAEYPHGG--------AMRPAAWCHEFGQGRVVYLMPGHHLPSFSV-E 196 Y P + I+AE GG + P AW +G+G+V ++ GH F V + Sbjct: 165 YMHYDPSIK--IIAETKFGGEVYPWIKDVIMPVAWKKIYGKGKVFFISIGHDPDEFMVYK 222 Query: 197 PFRKMIKRGGLWAA 210 +++ RG +WA Sbjct: 223 DGWELLTRGFMWAV 236 >gb|EAJ40229.1| unknown [environmental sequence] Length = 260 Score = 65.1 bits (157), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 56/216 (25%), Positives = 84/216 (38%), Gaps = 24/216 (11%) Query: 5 ELKALALGSYTEVKYHPFAGVDRVFEELLGEELQVQSTEDYSLLSKDSLSEYKLVIAYTE 64 ELK ++ KYH +LL E + +T + + D+ LVI YT Sbjct: 4 ELKPYSVHLICNAKYHDSDFARLEILKLLAEHDDIYTTVSDTYDNFDNHIGKNLVITYT- 62 Query: 65 FSDHKIPPEQSGGFLSYVASGGGLLVVH--NGI---------SLQRNQELGVMLGGHFTH 113 D K + ++ +GG +H N I + E +LG F Sbjct: 63 -CDLKPEDKDVKHMEEFLKNGGNWFALHGTNAILEFAGGKADTPNTCPEYMELLGSRFIS 121 Query: 114 HPAYTSLQMTVPATGHPIMEGITDFVMEDEPYYFDMQPHFETTILAEY-----------P 162 HP + V H + +GI DF + DEPYY + + AEY P Sbjct: 122 HPPNMDFLVRVKDKEHQLTKGIDDFEVYDEPYYCEYDDKIHVLLDAEYSEPAEAYVAVDP 181 Query: 163 HGGAMRPAAWCHEFGQGRVVYLMPGHHLPSFSVEPF 198 RP + H G+G+V+YL+ GH + + PF Sbjct: 182 PNDDPRPQMYLHNVGKGKVLYLLLGHCRGKYDMRPF 217 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 345,828,324 Number of extensions: 14656838 Number of successful extensions: 28937 Number of sequences better than 1.0e-05: 33 Number of HSP's gapped: 28932 Number of HSP's successfully gapped: 33 Length of query: 213 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 93 Effective length of database: 431,425,698 Effective search space: 40122589914 Effective search space used: 40122589914 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)