BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0479.0008.p (394 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_563831.1| amidase family protein [Arabidopsis thaliana] >... 238 1e-61 emb|CAE01284.1| OSJNBa0020P07.1 [Oryza sativa (japonica cultivar... 217 3e-55 gb|AAS47585.1| chloroplast Toc64-2 [Physcomitrella patens] 214 3e-54 ref|ZP_00055631.1| COG0154: Asp-tRNAAsn/Glu-tRNAGln amidotransfe... 214 4e-54 ref|ZP_00024293.1| COG0154: Asp-tRNAAsn/Glu-tRNAGln amidotransfe... 209 1e-52 >ref|NP_563831.1| amidase family protein [Arabidopsis thaliana] gb|AAG35612.1| amidase [Arabidopsis thaliana] gb|AAK59783.1| At1g08980/F7G19_15 [Arabidopsis thaliana] gb|AAO42800.1| At1g08980/F7G19_15 [Arabidopsis thaliana] Length = 425 Score = 238 bits (608), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 155/423 (36%), Positives = 205/423 (48%), Gaps = 35/423 (8%) Query: 1 MAVHNLYGAFIEP---ELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPS 57 MA +N +GAFIE T S L G +FA+KD+F V G + GNPDWLR+H + Sbjct: 1 MATNNDFGAFIEKVTISPTSTSSSPPSLQGLTFAIKDIFDVEGRVTGFGNPDWLRTHSAA 60 Query: 58 AAHSAVVSMLLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIPXXXXX-XXXX 116 + + VVS LL++GAT G T DE+ YS+ GEN HYGTP NP+A DR+P Sbjct: 61 TSTAPVVSSLLEAGATALGITIMDEMAYSINGENAHYGTPRNPIAFDRVPGGSSSGSAVA 120 Query: 117 XXXXXXDFALGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPE 176 DF++GTDTGGSVRVP++YCG++GFRP+HGAV G+ P+A FDTVGW Sbjct: 121 VAARLVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTVGWFARDTA 180 Query: 177 LLLQAGEVL-----LGAAAPGSR--GGAMGKLHIAPEAWXXXXXXXXXXXRNG------- 222 L + G VL L P KL P G Sbjct: 181 TLKRVGCVLLQQHHLNPIEPSQLIIADDCFKLCSVPHDLLVQPLVGSVEKSFGGNTVVKK 240 Query: 223 -------------LQLLQAGAGWSGATEIAPEGLKTWMNVFRELQGAEIWATHGAWIERA 269 L+ + E L + R LQ E HGAWI Sbjct: 241 VNLGEYIGQNVPSLKHFMTSDDVTTQQEFCIPSLMALSSSMRLLQRHEFKINHGAWISSV 300 Query: 270 QPLFGPDIXXXXXXXXXXXGQ--DHSRALRMRGEIAARLRQLLGEDSCIVLPTVPGPAPL 327 +P FGP I + DH R+++ E+ L LLGE +V+PTVPGP P Sbjct: 301 KPEFGPGISERIEEAIRTSDEKIDHCRSVK--SELITALSTLLGEKGVLVIPTVPGPPPH 358 Query: 328 RGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTLPVIGPAGLPLGLSVIGGHGQDLRLLSWV 387 ++A LE RS A L IAG++G QV++P+ LP+ +S++ +G D LLS V Sbjct: 359 LQANVAALESFRSRAFSLLSIAGVSGFCQVSIPLGLHENLPVSVSLVAKYGSDGFLLSLV 418 Query: 388 REI 390 + Sbjct: 419 DSL 421 >emb|CAE01284.1| OSJNBa0020P07.1 [Oryza sativa (japonica cultivar-group)] Length = 435 Score = 217 bits (553), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 145/413 (35%), Positives = 198/413 (47%), Gaps = 27/413 (6%) Query: 7 YGAFIEPELEVTGSGSGKLA-GFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVVS 65 YGAF+E + G +FA+KD+F +AG + GNPDW R+H P+AA S VV Sbjct: 11 YGAFMERFVLPPPPSQQLPLHGLTFAIKDIFDIAGRVTGFGNPDWARTHAPAAATSPVVL 70 Query: 66 MLLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIP-XXXXXXXXXXXXXXXDF 124 L +GAT G T DE+ YS+ GEN HYGTP NP A R+P DF Sbjct: 71 AALAAGATSLGTTIMDEMAYSINGENTHYGTPTNPCAPGRVPGGSSSGSAVAVAANLVDF 130 Query: 125 ALGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQAGEV 184 +LGTDTGGSVRVP+AYCG++G RP+HG V E VIP+A FDTVGW + L + +V Sbjct: 131 SLGTDTGGSVRVPAAYCGIFGLRPSHGLVSAENVIPMAQMFDTVGWFSRDLSTLSRVTKV 190 Query: 185 LLG-----AAAPGSRGGAMGKLHI--APEAWXXXXXXXXXXXRNGLQLLQAGAGWSGATE 237 LL P M I + + R Q+L ++ Sbjct: 191 LLPLPDDIVKQPTQVTIPMDCFQILGSLDDRTYQIINASVAKRFDSQILDNRNLGDFISD 250 Query: 238 IAPE-----------------GLKTWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXX 280 P L +V R LQ ++ A H W+ +P GP + Sbjct: 251 NVPSIGKFITDFSESELPSVPALSVISHVMRGLQRSQFKANHAEWVNTVKPNLGPGLRER 310 Query: 281 XXXXXXXXGQDHSRALR-MRGEIAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNR 339 + + +R E + L LL + + +PTVPGP P G + A LE R Sbjct: 311 ILEAIASGDNESLEDFQAIRAEFKSALAALLKDHGILAIPTVPGPPPKVGMEAAPLENFR 370 Query: 340 SGAMMLSCIAGLAGLPQVTLPVIGPAGLPLGLSVIGGHGQDLRLLSWVREIWQ 392 + A L IAGL+G QV++P+ GLP+ +S++ HG D LL+ V E++Q Sbjct: 371 ARAFSLLSIAGLSGFCQVSIPLGMRNGLPVSVSLVARHGADHFLLNVVEELYQ 423 >gb|AAS47585.1| chloroplast Toc64-2 [Physcomitrella patens] Length = 585 Score = 214 bits (545), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 132/384 (34%), Positives = 180/384 (46%), Gaps = 23/384 (5%) Query: 27 GFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHSAVVSMLLQSGATLRGATHTDELMYS 86 G +FAVKD+F V G + GNP W +H P+ + V +L+++GA G H DEL YS Sbjct: 63 GLTFAVKDIFDVEGFITGFGNPVWAETHEPATVTAPAVKVLVEAGAKCVGKLHMDELAYS 122 Query: 87 LGGENYHYGTPVNPLAKDRIPXXXXX-XXXXXXXXXXDFALGTDTGGSVRVPSAYCGVYG 145 + GEN HYGTPVNP A +RIP DF+LGTDTGGSVRVP+A+CG+ G Sbjct: 123 INGENKHYGTPVNPAAPNRIPGGSSSGSAVAVAANCVDFSLGTDTGGSVRVPAAFCGILG 182 Query: 146 FRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQAGEVLLGAAAPGSR-------GGAM 198 FRP+HGA+ + GV+P+A FDTVGW P+ L Q G LL S+ Sbjct: 183 FRPSHGAISVSGVLPMAQSFDTVGWFAKDPKTLRQVGHALLQLPYSDSKQPRRVLIADDC 242 Query: 199 GKLHIAPEAWXXXXXXXXXXXRNGLQLLQ-------AGAGWSGATEIAPE--------GL 243 KL + P G Q+LQ E+ E L Sbjct: 243 FKLSLIPNEDVVGAVIRSVQKLLGRQVLQYINLGDFIRRNVPSLKELEKEISNGSPIGAL 302 Query: 244 KTWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQDHSRALRMRGEIA 303 + LQ E H W+E A+P DI G S ++R E Sbjct: 303 TLLRTAMQLLQRWEFKENHQEWLENAKPDLSSDIAARVQAALDMKGDQVSLVQQIRNEAR 362 Query: 304 ARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTLPVIG 363 + LL D+ +V+PTVP P G ++ R+ A L ++G++G QV++PV Sbjct: 363 FAINDLLKNDTILVMPTVPDIPPKLGIKADFPDEFRARAFDLLSVSGMSGCCQVSVPVGE 422 Query: 364 PAGLPLGLSVIGGHGQDLRLLSWV 387 +P +S++ G D LL V Sbjct: 423 YNNVPTAVSLLAKRGSDRFLLDTV 446 >ref|ZP_00055631.1| COG0154: Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases [Magnetospirillum magnetotacticum] Length = 391 Score = 214 bits (544), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 142/390 (36%), Positives = 197/390 (50%), Gaps = 4/390 (1%) Query: 3 VHNLYGAF-IEPELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAHS 61 +H+ AF + ++ + G+ SG LAG +FA KDVF VAG+ + AGNPDW R P+ + Sbjct: 1 MHDPLNAFALGGDVYIDGAKSGPLAGLTFAAKDVFDVAGYVTGAGNPDWRRLAEPAKQTA 60 Query: 62 AVVSMLLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIP-XXXXXXXXXXXXX 120 VS LL+SGA L G THTDEL + G+N HYGTP NP A +P Sbjct: 61 WAVSALLESGARLVGKTHTDELTRGIFGDNSHYGTPDNPRAPGHVPGGSSSGSAAAVAGG 120 Query: 121 XXDFALGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLLQ 180 ALGTDT GS RVP+++CGVYG RPT G + M+GV+ + FDTVG + E+L Sbjct: 121 LASMALGTDTAGSTRVPASFCGVYGLRPTLGTIPMDGVLSQSNTFDTVGLLADDAEILGL 180 Query: 181 AGEVLLGAAAPGSRGGAMGKLHIAPEAWXXXXXXXXXXXRNGLQLLQAGAGWSGATEIAP 240 GE LL R L A EA ++ A A + +++P Sbjct: 181 MGEALLRKKIKDVRPAQAVVLEDAMEASDPAVAAAIEAALPRIK--DAVAPITRGRKVSP 238 Query: 241 EGLKTWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQDHSRALRMRG 300 L W+ +QG E W G WI + P FG ++ + S A R Sbjct: 239 VPLADWVEHQNAIQGREAWERFGEWINNSNPRFGFEVADNFLRGSKVSQRTLSAARGFRL 298 Query: 301 EIAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTLP 360 +++ L ++ +VLPT P AP + + + R + L+ IAG+AG PQ++LP Sbjct: 299 RAKRWVQEALEGNAVLVLPTTPVTAPPVHSPRSVMWEIRHRMVSLTTIAGMAGCPQISLP 358 Query: 361 VIGPAGLPLGLSVIGGHGQDLRLLSWVREI 390 + GLP+GLS+IG G D LL+ R I Sbjct: 359 LCKVDGLPVGLSLIGPRGSDALLLAAARRI 388 >ref|ZP_00024293.1| COG0154: Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases [Ralstonia metallidurans] Length = 396 Score = 209 bits (531), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 131/386 (33%), Positives = 188/386 (48%), Gaps = 3/386 (0%) Query: 1 MAVHNLYGAFIEPELEVTGSGSGKLAGFSFAVKDVFAVAGHCSSAGNPDWLRSHPPSAAH 60 +A H P+ V + +G L G +FAVKD+F V+G+ + GNP L Sbjct: 3 LATHPSRAFLPYPDAPVPNAANGPLRGLTFAVKDLFDVSGYPTGGGNPHVLARSGIKTTT 62 Query: 61 SAVVSMLLQSGATLRGATHTDELMYSLGGENYHYGTPVNPLAKDRIPXXXXXXXXXXXXX 120 + V LL +GA G HTDEL +S+ G+N+HYG P N A DRI Sbjct: 63 APTVQKLLDAGAAFVGKVHTDELAFSMNGQNHHYGGPYNGAAPDRITGGSSSGSASAVSN 122 Query: 121 XX-DFALGTDTGGSVRVPSAYCGVYGFRPTHGAVEMEGVIPLAPGFDTVGWITACPELLL 179 DFALGTDTGGSVR P+++CG++G RP+HG + + +PL DT G+ Sbjct: 123 KLCDFALGTDTGGSVRAPASHCGLFGIRPSHGRISLTHCMPLCETLDTCGFFARDIATFA 182 Query: 180 QAGEVLLGAAAPGSRGGAMGKLHIAPEAWXXXXXXXXXXXRNGLQLLQAGAGWSGATEIA 239 + +VLLGA A + + +L +A + + ++A G + ++A Sbjct: 183 RVADVLLGADA--NPLPSRPRLLLAADLFELPTPEARKALAPATARIEAVLGKASPVDVA 240 Query: 240 PEGLKTWMNVFRELQGAEIWATHGAWIERAQPLFGPDIXXXXXXXXXXXGQDHSRALRMR 299 L FR +QG E W GA IE GPD+ ++ +R Sbjct: 241 DRPLSDLYWAFRYVQGWEAWRADGALIENYGLQLGPDVAGRFAFSKEVTAAQFEKSSAVR 300 Query: 300 GEIAARLRQLLGEDSCIVLPTVPGPAPLRGGDLAQLEQNRSGAMMLSCIAGLAGLPQVTL 359 E A L LLG+D ++LPT+P APLR + LE R+ A C+ L G PQ++L Sbjct: 301 REFTAHLGCLLGQDGILLLPTMPDIAPLRTTPMEALEDYRTTAAHTLCLTPLTGFPQLSL 360 Query: 360 PVIGPAGLPLGLSVIGGHGQDLRLLS 385 P+ G G PLG+S+IG G D L++ Sbjct: 361 PLSGRDGAPLGVSLIGPMGSDRSLIA 386 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 602,355,519 Number of extensions: 24508104 Number of successful extensions: 56173 Number of sequences better than 1.0e-05: 995 Number of HSP's gapped: 55273 Number of HSP's successfully gapped: 1006 Length of query: 394 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 266 Effective length of database: 409,704,906 Effective search space: 108981504996 Effective search space used: 108981504996 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)