BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0072.p (475 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_844382.1| sensor histidine kinase [Bacillus anthracis str... 290 6e-77 ref|NP_831743.1| Two component system histidine kinase [Bacillus... 288 2e-76 ref|NP_563155.1| two-component sensor histidine kinase [Clostrid... 286 6e-76 ref|NP_978375.1| sensor histidine kinase [Bacillus cereus ATCC 1... 286 9e-76 ref|NP_782523.1| alkaline phosphatase synthesis sensor protein p... 270 4e-71 >ref|NP_844382.1| sensor histidine kinase [Bacillus anthracis str. Ames] gb|AAP25868.1| sensor histidine kinase [Bacillus anthracis str. Ames] Length = 453 Score = 290 bits (741), Expect = 6e-77, Method: Compositional matrix adjust. Identities = 170/468 (36%), Positives = 276/468 (58%), Gaps = 28/468 (5%) Query: 6 MRRQIVLHYXXXXXXXXXXXXXXXXXXXRTYYYDSVYSKITTHIGTAEDFLK-------- 57 M+R++ L++ YYY+ + + +H T+ F Sbjct: 1 MKRKVTLYFMTVIILMLVLFEVVFSVSVYRYYYNGIVQYVESHAKTSTRFFSEYNSLYFI 60 Query: 58 --YEISGERSPNQLQKLLDFFEMDYTELQVLTQDGNMLISSTGFQPDRTITTSDVPEAV- 114 E SG+ +++ F+++ TELQ++ + G ++ SS+G + + + +P ++ Sbjct: 61 RLQEYSGD--------IIESFQLEGTELQLIDRHGTIIQSSSGEKTEGKVV---IPYSLL 109 Query: 115 GGSVGRWVGRQPGTNESVMAVSKLVHINGRETYVIRYVTSMERIDSDLLNLTLVSIGIGS 174 G + V + + +S L+H G+ V++Y T + +++ ++ + + +I +G Sbjct: 110 EGEMYHQVTTTKDKVKQLEVISPLIH-QGQTIGVLKYTTVLTHVNAKIIEIIMFTICVGI 168 Query: 175 AVMAIVVLFSFGLANSIVKPLKNITAVSAQMAKGKFTTRIKGDYRYEIGDLASTLNYMAD 234 + IV L S LANS VKP+++I S+Q+A+G +IK DY E+G+LA +LN+M+D Sbjct: 169 VISGIVFLISRRLANSFVKPIESIIHASSQIAEGTLKKKIKEDYPGELGELAHSLNHMSD 228 Query: 235 EIIRSNQIKDDFISSISHELRTPLTSIKGWSETLVSGGY-DPEETKLGMGIISKESDRLI 293 +I ++ Q+K++FI+SISHE+RTPLT IKGWSETL + + EE K GMGIIS E+DRLI Sbjct: 229 KIEKAEQMKNEFIASISHEIRTPLTGIKGWSETLKTVDHLTEEEIKQGMGIISGETDRLI 288 Query: 294 GLVEEILDFSKLQQNEMKLSMGRVDIKVLLQETILNIWAKAEKKRIHLLLECEETFFIKG 353 LVEE+LDFS+LQ N L +V + +L+ETI + AE+K++ + E I G Sbjct: 289 HLVEELLDFSRLQSNHFNLYKQKVQLYDILEETIWQLTPNAEEKKMQFIKTIERIELI-G 347 Query: 354 DANRLKQVFLNLVDNAVKFSPEDSSIVLSAQRLTGSELAVI-VRDSGIGISEAHLGRVRD 412 D NRLKQ+FLN+V NA+K+S E+ + + A + G AVI V+D GIGI++ HL + Sbjct: 348 DRNRLKQIFLNIVQNAIKYSHENGKVYIEATKNEGQ--AVIKVKDDGIGIAKEHLPYIEQ 405 Query: 413 RFFQVDALNGGTGLGLAISQQIVELHGGKLEMDSELGKGTQVTVILPM 460 F+Q++ G GLGLAI +++VELHGG + + S+ G GT + + LP+ Sbjct: 406 SFYQINNHATGAGLGLAIVKKMVELHGGTINIISKEGIGTTILIKLPL 453 >ref|NP_831743.1| Two component system histidine kinase [Bacillus cereus ATCC 14579] gb|AAP08944.1| Two component system histidine kinase [Bacillus cereus ATCC 14579] Length = 443 Score = 288 bits (736), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 170/438 (38%), Positives = 270/438 (61%), Gaps = 29/438 (6%) Query: 36 YYYDSVYSKITTHIGTAEDFLK----------YEISGERSPNQLQKLLDFFEMDYTELQV 85 YYY+ + I +H T+ F E SG+ ++ F+++ TELQ+ Sbjct: 22 YYYNGIVQYIESHAKTSTRFFSEYNSLYFIRLQEYSGD--------IISSFQLEGTELQL 73 Query: 86 LTQDGNMLISSTGFQPDRTITTSDVPEAV-GGSVGRWVGRQPGTNESVMAVSKLVHINGR 144 + + G+++ SS+G + + + ++P+++ G + V + G + + +S L+H G+ Sbjct: 74 IDRHGSVIQSSSGEKVEGKV---NIPQSLLEGEMYNQVTTK-GNKKQLEVLSPLIH-QGQ 128 Query: 145 ETYVIRYVTSMERIDSDLLNLTLVSIGIGSAVMAIVVLFSFGLANSIVKPLKNITAVSAQ 204 V++Y T + ++ ++ + +I +G + +V L S LANS V+P+++I S+Q Sbjct: 129 TIGVLKYTTVLTNVNKKIIEIITFTISVGIVISGVVFLISRRLANSFVEPIESIIQASSQ 188 Query: 205 MAKGKFTTRIKGDYRYEIGDLASTLNYMADEIIRSNQIKDDFISSISHELRTPLTSIKGW 264 +A+G +IK DY E+GDLA +LNYMA +I ++ Q+K++FI+SISHE+RTPLT IKGW Sbjct: 189 IAEGTLKQKIKEDYPGELGDLAHSLNYMAYKIDKAEQMKNEFIASISHEIRTPLTGIKGW 248 Query: 265 SETLVSGGY-DPEETKLGMGIISKESDRLIGLVEEILDFSKLQQNEMKLSMGRVDIKVLL 323 SETL + + EE K GMGIIS E+DRLI LVEE+LDFS+LQ N L +V + +L Sbjct: 249 SETLKTVDHLTEEEIKQGMGIISGETDRLIHLVEELLDFSRLQSNHFNLYKQKVQLYDIL 308 Query: 324 QETILNIWAKAEKKRIHLLLECEETFFIKGDANRLKQVFLNLVDNAVKFSPEDSSIVLSA 383 +ETI + K+E+K I + + + GD NRLKQVFLN+V NA+K+S + S+I + A Sbjct: 309 EETIWQLTPKSEEKEIQFITTIDRMELM-GDRNRLKQVFLNIVQNAIKYSHKKSTIHIEA 367 Query: 384 QRLTGSELAVI-VRDSGIGISEAHLGRVRDRFFQVDALNGGTGLGLAISQQIVELHGGKL 442 ++ G AVI VRD GIGI++ HL + F+Q++ G G+GLAI +++VELH G L Sbjct: 368 TKIEGQ--AVIKVRDEGIGIAKEHLPYIEQSFYQINNHAAGAGIGLAIVKKMVELHSGVL 425 Query: 443 EMDSELGKGTQVTVILPM 460 + S+ G GT + + LP+ Sbjct: 426 SVTSKEGIGTTILIKLPL 443 >ref|NP_563155.1| two-component sensor histidine kinase [Clostridium perfringens] dbj|BAB81945.1| two-component sensor histidine kinase [Clostridium perfringens str. 13] Length = 471 Score = 286 bits (733), Expect = 6e-76, Method: Compositional matrix adjust. Identities = 167/428 (39%), Positives = 253/428 (59%), Gaps = 6/428 (1%) Query: 36 YYYDSVYSKITTHIGTAEDFLKYEISGERSPNQLQKLLD-FFEMDYTELQVLTQDGNMLI 94 YYY + + + I + DF K +S + + +D F++ ++Q+ G +++ Sbjct: 34 YYYQNTEEILLSQINISVDFYKRYLSNVSLEENVYEDVDIFWKQTDAQVQIYNLKGQLIM 93 Query: 95 SSTGFQPDRTITTSDVPEAVGGSVGRWVGRQPGTNESVMAVSKLVHINGRETY-VIRYVT 153 S G +P T DV A+ G +WVG P VMA+S + + E VIRY++ Sbjct: 94 DSIGLEPKEYNTPVDVKRALEGDTAKWVGTVPDYTGKVMAISAPLRNSSNEIVGVIRYIS 153 Query: 154 SMERIDSDLLNLTLVSIGIGSAVMAIVVLFSFGLANSIVKPLKNITAVSAQMAKGKFTTR 213 S+ +D+ +LN LV + IG V+AI ++ S+ LANSIV P+ + VS QMAKG R Sbjct: 154 SLRNVDNFILNFFLVFLVIGLTVLAIGIILSYFLANSIVNPITELIKVSEQMAKGNLKVR 213 Query: 214 IKGDYRYEIGDLASTLNYMADEIIRSNQIKDDFISSISHELRTPLTSIKGWSETLVSGGY 273 K EI LA +LN MA+EI +K++FISS+SHELRTPLTSIKGW+ TL + Sbjct: 214 NKIVTNDEIEKLADSLNIMAEEIENREILKNEFISSVSHELRTPLTSIKGWAITLNNDFT 273 Query: 274 DPEETKLGMGIISKESDRLIGLVEEILDFSKLQQNEMKLSMGRVDIKVLLQETILNIWAK 333 D E K+G II KE+DRL +VEE+LDFSK ++KL +++K ++ L + + Sbjct: 274 DRETLKMGFDIIEKEADRLSNMVEELLDFSKFVSGKIKLKYEEINLKEFIEYLRLYMNPR 333 Query: 334 AEKKRIHLLLE-CEETFFIKGDANRLKQVFLNLVDNAVKFSPEDSSIVLSAQRLTGSE-L 391 AE++ L+L+ E F I GD +RLKQVF+N++DNA KF+ E+ I + + + E + Sbjct: 334 AEREHKELILKGVTEDFIIVGDKDRLKQVFINIIDNAFKFTHENEKITI--EFVYADEGI 391 Query: 392 AVIVRDSGIGISEAHLGRVRDRFFQVDALNGGTGLGLAISQQIVELHGGKLEMDSELGKG 451 + + D+G GIS+ L RV+++F++ G+GL+I +I+ LH G LE+ SELG G Sbjct: 392 YINIIDTGCGISKEELPRVKEKFYKGKNSKSQNGIGLSICDEIIALHEGTLEIYSELGNG 451 Query: 452 TQVTVILP 459 T+V + LP Sbjct: 452 TKVVIYLP 459 >ref|NP_978375.1| sensor histidine kinase [Bacillus cereus ATCC 10987] gb|AAS40983.1| sensor histidine kinase [Bacillus cereus ATCC 10987] Length = 453 Score = 286 bits (731), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 169/468 (36%), Positives = 275/468 (58%), Gaps = 28/468 (5%) Query: 6 MRRQIVLHYXXXXXXXXXXXXXXXXXXXRTYYYDSVYSKITTHIGTAEDFLK-------- 57 M+R++ L++ YYY+ + I +H T+ F Sbjct: 1 MKRKVTLYFMTVIILMLVLFEVVFSVSIYRYYYNGIVQYIESHAKTSTRFFSEYNSLYFI 60 Query: 58 --YEISGERSPNQLQKLLDFFEMDYTELQVLTQDGNMLISSTGFQPDRTITTSDVPEAV- 114 E SG+ +++ F+++ TELQ++ + G ++ SS+G + I +P ++ Sbjct: 61 RLQEYSGD--------IIESFQLEGTELQLIDRHGTIIQSSSGQKVKGKIV---IPYSLL 109 Query: 115 GGSVGRWVGRQPGTNESVMAVSKLVHINGRETYVIRYVTSMERIDSDLLNLTLVSIGIGS 174 G + V + + +S L+H G+ V++Y T + +++ ++ + + +I +G Sbjct: 110 EGEMYHQVATTKDNVKQLEVISPLIH-QGQTIGVLKYTTVLTNVNAKIIEIIMFTISVGI 168 Query: 175 AVMAIVVLFSFGLANSIVKPLKNITAVSAQMAKGKFTTRIKGDYRYEIGDLASTLNYMAD 234 + IV L S LANS V+P+++I S+Q+A+G +IK DY E+G+LA +LN+M++ Sbjct: 169 VISGIVFLISRRLANSFVRPIESIIHASSQIAEGTLKKKIKEDYPGELGELAHSLNHMSN 228 Query: 235 EIIRSNQIKDDFISSISHELRTPLTSIKGWSETLVSGGY-DPEETKLGMGIISKESDRLI 293 +I ++ Q+K++FI+SISHE+RTPLT IKGWSETL + + EE K GMGIIS E+DRLI Sbjct: 229 KIEKAEQMKNEFIASISHEIRTPLTGIKGWSETLKTVDHLTEEEIKQGMGIISGETDRLI 288 Query: 294 GLVEEILDFSKLQQNEMKLSMGRVDIKVLLQETILNIWAKAEKKRIHLLLECEETFFIKG 353 LVEE+LDFS+LQ N L +V + +L+ETI + AE+K++ + E I G Sbjct: 289 HLVEELLDFSRLQSNHFNLYKQKVQLYDILEETIWQLTPNAEEKQMQFIKTIERIELI-G 347 Query: 354 DANRLKQVFLNLVDNAVKFSPEDSSIVLSAQRLTGSELAVI-VRDSGIGISEAHLGRVRD 412 D NRLKQ+FLN+V NA+K+S ++ + + A + G AVI V+D GIGI++ HL + Sbjct: 348 DRNRLKQIFLNIVQNAIKYSHKNGKVYIEATKNEGQ--AVIKVKDEGIGIAKEHLPYIEQ 405 Query: 413 RFFQVDALNGGTGLGLAISQQIVELHGGKLEMDSELGKGTQVTVILPM 460 F+Q++ G GLGLAI +++VELHGG + + S+ G GT + + LP+ Sbjct: 406 SFYQINNHATGAGLGLAIVKKMVELHGGTINIISKEGIGTTILIKLPL 453 >ref|NP_782523.1| alkaline phosphatase synthesis sensor protein phoR [Clostridium tetani E88] gb|AAO36460.1| alkaline phosphatase synthesis sensor protein phoR [Clostridium tetani E88] Length = 471 Score = 270 bits (691), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 150/436 (34%), Positives = 262/436 (60%), Gaps = 8/436 (1%) Query: 34 RTYYYDSVYSKITTHIGTAEDFLKYEISGERSPNQLQKLLD-FFEMDYTELQVLTQDGNM 92 + YYY ++ +T + + DF S + +D F++ ++Q++ G + Sbjct: 32 KRYYYGNLEEMLTNQVKLSSDFYSRYFSNTTLEENIMDNVDVFWKQTNAQVQIINTQGIV 91 Query: 93 LISSTGFQPDRTITTSDVPEAVGGSVGRWVGRQPGTNESVMAVSKLVHINGRETYVIRYV 152 L+ S G I T+D A+ G G WVG VM+VS + ++ + ++RYV Sbjct: 92 LLDSIGATTKEVIKTADFKNALEGDKGIWVGDVSYDKYRVMSVSYPIKVDNKIIGILRYV 151 Query: 153 TSMERIDSDLLNLTLVSIGIGSAVMAIVVLFSFGLANSIVKPLKNITAVSAQMAKGKFTT 212 +S++ ID ++ ++++ IG V+ +V +FS LA++++ P+K +T V+ +MA G Sbjct: 152 SSLKEIDKAIVKMSVLFFSIGLIVIFVVGIFSVFLADTVIGPIKKLTKVAEEMADGNLEI 211 Query: 213 RIKGDYRYEIGDLASTLNYMADEIIRSNQIKDDFISSISHELRTPLTSIKGWSETLVSGG 272 R + ++ EIG L+ TLNYMA+EI++ +++K++FISSISHE+RTPLTSIKGW+ TL Sbjct: 212 RNEKEFDDEIGRLSDTLNYMAEEILKKDKLKNEFISSISHEIRTPLTSIKGWAITL--NT 269 Query: 273 YDPEETKL---GMGIISKESDRLIGLVEEILDFSKLQQNEMKLSMGRVDIKVLLQETILN 329 D E+ L G+ II +ES+RL +VEE+LDFSKL ++ + +VD+ ++ Sbjct: 270 EDLEDKNLIRDGLNIIEEESERLSKMVEELLDFSKLISGKVTIKKEKVDLNEVISYIEKY 329 Query: 330 IWAKAEKKRIHLLLECEETFFIKG-DANRLKQVFLNLVDNAVKFSPED-SSIVLSAQRLT 387 + +A+++ + ++E I D +R+KQVF+N++DNA KFS E+ VL + ++ Sbjct: 330 LSPRAKRENVQFIVEKNYDLPIMYIDVDRMKQVFINILDNAFKFSKEEGEKRVLLSIKIE 389 Query: 388 GSELAVIVRDSGIGISEAHLGRVRDRFFQVDALNGGTGLGLAISQQIVELHGGKLEMDSE 447 E+ +++ D+G GIS++ L +++++F++ G+GL+I +IV +HGG LE DSE Sbjct: 390 NEEVIILIEDNGYGISKSDLPKIKEKFYKGKNSKSQNGIGLSICDEIVAMHGGTLEFDSE 449 Query: 448 LGKGTQVTVILPMTEE 463 + +GT+V + LP +E Sbjct: 450 INRGTKVYIKLPYKDE 465 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.135 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 637,447,741 Number of extensions: 25762432 Number of successful extensions: 99152 Number of sequences better than 1.0e-05: 5631 Number of HSP's gapped: 87370 Number of HSP's successfully gapped: 5659 Length of query: 475 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 346 Effective length of database: 406,989,807 Effective search space: 140818473222 Effective search space used: 140818473222 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)