BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0476.0062.p
         (99 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF 
           2,715,099 sequences; 757,237,578 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_349775.1| Prolyl-tRNA synthetase [Clostridium acetobutyli...    73   2e-12
ref|ZP_00106959.1| COG0442: Prolyl-tRNA synthetase [Nostoc punct...    72   4e-12
ref|NP_563344.1| proline-tRNA ligase [Clostridium perfringens] >...    72   4e-12
ref|NP_895087.1| tRNA synthetases, class-II (G, H, P and S):Prol...    72   5e-12
ref|ZP_00164978.1| hypothetical protein Selo116501 [Synechococcu...    72   5e-12

>ref|NP_349775.1| Prolyl-tRNA synthetase [Clostridium acetobutylicum]
 pir||H97290 prolyl-tRNA synthetase [imported] - Clostridium acetobutylicum
 gb|AAK81115.1| Prolyl-tRNA synthetase [Clostridium acetobutylicum]
          Length = 570

 Score = 72.8 bits (177), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 35/62 (56%), Positives = 47/62 (75%)

Query: 23  GIEVLLDDRDERAGVKFKDSILMGIPVTIVVGKAAAEGKVEFMDRGSNSKEVIGIAEAVS 82
           G++ LLDDR+ERAGVKFKD+ L+GIP+ I VGK  +EGKVEF  R S   EVI + + ++
Sbjct: 497 GVDALLDDRNERAGVKFKDADLIGIPMRITVGKKISEGKVEFKCRNSEEVEVIDLDKVIA 556

Query: 83  RI 84
           R+
Sbjct: 557 RV 558


>ref|ZP_00106959.1| COG0442: Prolyl-tRNA synthetase [Nostoc punctiforme]
          Length = 604

 Score = 72.0 bits (175), Expect = 4e-12,   Method: Composition-based stats.
 Identities = 40/89 (44%), Positives = 51/89 (57%)

Query: 2   SIQDEVQTXXXXXXXXXXXXXGIEVLLDDRDERAGVKFKDSILMGIPVTIVVGKAAAEGK 61
           +I+D  Q              GIE LLDDRDERAGVKFKD+ L+GIP  IV G+A A GK
Sbjct: 510 NIKDAQQVEVAQKLYTELNQAGIETLLDDRDERAGVKFKDADLIGIPYRIVTGRAIANGK 569

Query: 62  VEFMDRGSNSKEVIGIAEAVSRITSGGTA 90
           VE ++R +   + I I E  + +    TA
Sbjct: 570 VEVVERATRKSQEIVIDEVTTTLQQWITA 598


>ref|NP_563344.1| proline-tRNA ligase [Clostridium perfringens]
 dbj|BAB82134.1| proline-tRNA ligase [Clostridium perfringens str. 13]
          Length = 570

 Score = 72.0 bits (175), Expect = 4e-12,   Method: Compositional matrix adjust.
 Identities = 38/83 (45%), Positives = 48/83 (57%)

Query: 2   SIQDEVQTXXXXXXXXXXXXXGIEVLLDDRDERAGVKFKDSILMGIPVTIVVGKAAAEGK 61
           ++++E Q              G+E LLDDR ERAGVKFKDS LMGIP+ I VGK   EG+
Sbjct: 477 NVKNEEQATKAEEIYNELRKMGVEALLDDRKERAGVKFKDSELMGIPMRITVGKMIGEGQ 536

Query: 62  VEFMDRGSNSKEVIGIAEAVSRI 84
           VEF  R     E + I E  +R+
Sbjct: 537 VEFKLRNGGEVETLSIEEVYNRV 559


>ref|NP_895087.1| tRNA synthetases, class-II (G, H, P and S):Prolyl-tRNA synthe...
           [Prochlorococcus marinus str. MIT 9313]
 emb|CAE21434.1| tRNA synthetases, class-II (G, H, P and S):Prolyl-tRNA synthetase
           [Prochlorococcus marinus str. MIT 9313]
          Length = 599

 Score = 71.6 bits (174), Expect = 5e-12,   Method: Composition-based stats.
 Identities = 37/83 (44%), Positives = 51/83 (61%)

Query: 2   SIQDEVQTXXXXXXXXXXXXXGIEVLLDDRDERAGVKFKDSILMGIPVTIVVGKAAAEGK 61
           +IQDEVQ               IE LLDDR ERAGVKFKD+ L+GIP  +VVG+ AA+G+
Sbjct: 505 NIQDEVQQKLAEQLYSELQAADIEALLDDRTERAGVKFKDADLIGIPWRVVVGRDAAKGQ 564

Query: 62  VEFMDRGSNSKEVIGIAEAVSRI 84
           VE + R S   +++   +A + +
Sbjct: 565 VELIQRSSRKVQILSAKQAFTAL 587


>ref|ZP_00164978.1| hypothetical protein Selo116501 [Synechococcus elongatus PCC 7942]
          Length = 600

 Score = 71.6 bits (174), Expect = 5e-12,   Method: Compositional matrix adjust.
 Identities = 38/83 (45%), Positives = 46/83 (55%)

Query: 2   SIQDEVQTXXXXXXXXXXXXXGIEVLLDDRDERAGVKFKDSILMGIPVTIVVGKAAAEGK 61
           +I D  Q              GIE LLDDRDERAGVKFKD+ L+GIP  IV G++  EGK
Sbjct: 509 NITDTEQVAAAEKIYADLTAAGIETLLDDRDERAGVKFKDADLIGIPYRIVTGRSLKEGK 568

Query: 62  VEFMDRGSNSKEVIGIAEAVSRI 84
           VE + R S    VI +   V  +
Sbjct: 569 VEVVQRASKESSVIAVGSVVETV 591


  Database: All non-redundant GenBank CDS
  translations+PDB+SwissProt+PIR+PRF
    Posted date:  Mar 24, 2004  1:53 AM
  Number of letters in database: 757,237,578
  Number of sequences in database:  2,715,099
  
Lambda     K      H
   0.315    0.135    0.360 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 2715099
Number of Hits to DB: 113,395,598
Number of extensions: 3670926
Number of successful extensions: 7363
Number of sequences better than 1.0e-05: 117
Number of HSP's gapped: 7408
Number of HSP's successfully gapped: 117
Length of query: 99
Length of database: 757,237,578
Length adjustment: 66
Effective length of query: 33
Effective length of database: 578,041,044
Effective search space: 19075354452
Effective search space used: 19075354452
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 120 (50.8 bits)