BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0476.0061.p (488 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_833539.1| Prolyl-tRNA synthetase [Bacillus cereus ATCC 14... 498 e-139 ref|NP_657787.1| tRNA-synt_2b, tRNA synthetase class II core dom... 496 e-139 ref|NP_623856.1| Prolyl-tRNA synthetase [Thermoanaerobacter teng... 494 e-138 ref|NP_980157.1| prolyl-tRNA synthetase [Bacillus cereus ATCC 10... 494 e-138 ref|NP_243285.1| prolyl-tRNA synthetase [Bacillus halodurans] >g... 489 e-137 >ref|NP_833539.1| Prolyl-tRNA synthetase [Bacillus cereus ATCC 14579] gb|AAP10740.1| Prolyl-tRNA synthetase [Bacillus cereus ATCC 14579] Length = 566 Score = 498 bits (1283), Expect = e-139, Method: Compositional matrix adjust. Identities = 245/464 (52%), Positives = 328/464 (70%), Gaps = 11/464 (2%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 M+QS + + TLRE PA+AE +HQLLLRAG++RQ A+GIY++LP G KVL K+E IVREE Sbjct: 1 MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 M+ AGA ELLMP+MQ AELW+ES R+ YG ELM++ DR+ REF LG THEEVIT L+R Sbjct: 61 MERAGAVELLMPAMQAAELWQESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRD 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 E+ SY+KLP+T+YQI TKFRDE+RPR GL+RGREFLMKDAYSF E LD+ Y R+YKA Sbjct: 121 EVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLYKA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEV- 242 Y IF RCGL +RAV AD+GA+GG+ +THEFM +S +GEDT+A DYAAN+E A V Sbjct: 181 YSNIFARCGLNFRAVIADSGAMGGK-DTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVV 239 Query: 243 --HTEAEAVQRNVSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVL 300 +T+++ ++ A EK TP + I+++ L I D+ +K+++++ K VL Sbjct: 240 ATYTKSDEAEK------ALEKVATPDQKAIEEVSAFLNIEADKCIKSMVFKVDEKLVVVL 293 Query: 301 VRGDHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAM 360 VRGDHEVN+VKV+N GAS + LA E V+A E G +GP+ + ++ D AVA++ Sbjct: 294 VRGDHEVNDVKVKNVYGASVVELASHEEVKALLNWEVGSLGPINVTGDIEIIADHAVASI 353 Query: 361 TSGITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIF 420 +G +GA E FH +V P RDF + D R +EG+ P G G + F +GIE+GH+F Sbjct: 354 VNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSP-DGNGTILFARGIEVGHVF 412 Query: 421 KLGTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLVXXXXLQW 464 KLGT+YSE + A+ LD NG+++ +IMGCYGIG+SR V Q+ Sbjct: 413 KLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQF 456 >ref|NP_657787.1| tRNA-synt_2b, tRNA synthetase class II core domain (G, H, P, S and T) [Bacillus anthracis A2012] ref|NP_846200.1| prolyl-tRNA synthetase [Bacillus anthracis str. Ames] gb|AAP27686.1| prolyl-tRNA synthetase [Bacillus anthracis str. Ames] Length = 566 Score = 496 bits (1276), Expect = e-139, Method: Compositional matrix adjust. Identities = 243/464 (52%), Positives = 327/464 (70%), Gaps = 11/464 (2%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 M+QS + + TLRE PA+AE +HQLLLRAG++RQ A+GIY++LP G KVL K+E IVREE Sbjct: 1 MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 M+ AGA ELLMP+MQ AELW+ES R+ YG ELM++ DR+ REF LG THEEVIT L+R Sbjct: 61 MERAGAVELLMPAMQAAELWQESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRD 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 E+ SY+KLP+T+YQI TKFRDE+RPR GL+RGREFLMKDAYSF E LD+ Y R+YKA Sbjct: 121 EVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLYKA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEV- 242 Y IF RCGL +RAV AD+GA+GG+ +THEFM +S +GEDT+A DYAAN+E A V Sbjct: 181 YSNIFARCGLNFRAVIADSGAMGGK-DTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVV 239 Query: 243 --HTEAEAVQRNVSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVL 300 +T+++ ++ + EK TP + I+++ L I D+ +K+++++ K VL Sbjct: 240 ATYTKSDEAEKEL------EKVATPDQKAIEEVSAFLNIEADKCIKSMVFKVDEKLVVVL 293 Query: 301 VRGDHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAM 360 VRGDHEVN+VKV+N GAS + LA E V+ E G +GP+ + ++ D AVA++ Sbjct: 294 VRGDHEVNDVKVKNVYGASVVELASHEEVKELLNCEVGSLGPIGVNGDIEIIADHAVASI 353 Query: 361 TSGITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIF 420 +G +GA E FH +V P RDF + D R +EG+ P G G + F +GIE+GH+F Sbjct: 354 VNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSP-DGNGTILFARGIEVGHVF 412 Query: 421 KLGTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLVXXXXLQW 464 KLGT+YSE + A+ LD NG+++ +IMGCYGIG+SR V Q+ Sbjct: 413 KLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQF 456 >ref|NP_623856.1| Prolyl-tRNA synthetase [Thermoanaerobacter tengcongensis] gb|AAM25460.1| Prolyl-tRNA synthetase [Thermoanaerobacter tengcongensis] Length = 573 Score = 494 bits (1273), Expect = e-138, Method: Compositional matrix adjust. Identities = 241/456 (52%), Positives = 324/456 (71%), Gaps = 9/456 (1%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 MR S LL TLRE PA+AE +H L+L+AG +R+LAAGIY YLPLG++VL+K+E IVREE Sbjct: 1 MRLSQLLMPTLREVPADAEIPSHVLMLKAGLMRKLAAGIYVYLPLGKRVLKKVEEIVREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 MD G+QE+LM ++ PAEL+KE+ R++V+G E+ KL DR++R+F LGPTHEEV T L+R Sbjct: 61 MDREGSQEVLMSALIPAELFKETGRWDVFGPEMFKLKDRNERDFCLGPTHEEVFTDLVRN 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 E+ SYR+LP+ +YQI TKFRDERRPR G+MR REF+MKDAYSFD WEGLD+++++MY+A Sbjct: 121 EVKSYRQLPLILYQIQTKFRDERRPRFGVMRSREFIMKDAYSFDADWEGLDESFNKMYRA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEVH 243 Y RIF+RCGL Y V AD GA+GG+ ++ EFM IS +GE +A C +C YAAN E+AE Sbjct: 181 YCRIFDRCGLKYLVVEADPGAMGGR-DSKEFMVISSVGEAVIAYCDSCGYAANEEKAECL 239 Query: 244 TEAEAVQRNVSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVLVRG 303 E+ + EK +TP ++TI++L L+I+P + +KTLIY+ GK A LVRG Sbjct: 240 IESRD-----EEMLEIEKVYTPNVKTIEELVDFLKISPSKFVKTLIYKAKGKVVAALVRG 294 Query: 304 DHEVNEVKVRNY--IGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAMT 361 D ++NE K+ N I E+ LAD V+ + G+ GP+ VTL+VD + + Sbjct: 295 DRDINETKLLNVLSIREEELELADGALVEEVTGAKVGFAGPLGLKGEVTLVVDSEIPQLR 354 Query: 362 SGITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIFK 421 + I GA E D+H+++V GRDF + VAD ++ EG+ CP CG L +GIE+GHIFK Sbjct: 355 NFIVGANETDYHIKNVNYGRDFKGDIVADIKSVVEGDRCPRCG-APLKIARGIEVGHIFK 413 Query: 422 LGTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLV 457 LGTKYSE LGA+ D G K ++MGCYGIGI+R V Sbjct: 414 LGTKYSEALGATYTDEEGNEKPIVMGCYGIGINRTV 449 >ref|NP_980157.1| prolyl-tRNA synthetase [Bacillus cereus ATCC 10987] gb|AAS42765.1| prolyl-tRNA synthetase [Bacillus cereus ATCC 10987] Length = 566 Score = 494 bits (1273), Expect = e-138, Method: Compositional matrix adjust. Identities = 243/464 (52%), Positives = 327/464 (70%), Gaps = 11/464 (2%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 M+QS + + TLRE PA+AE +HQLLLRAG++RQ A+GIY++LP G KVL K+E IVREE Sbjct: 1 MKQSMVFSPTLREVPADAEIKSHQLLLRAGFMRQNASGIYSFLPFGLKVLHKVERIVREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 M+ AGA ELLMP+MQ AELW+ES R+ YG ELM++ DR+ REF LG THEEVIT L+R Sbjct: 61 MERAGAVELLMPAMQAAELWQESGRWYSYGSELMRMKDRNAREFALGATHEEVITDLVRD 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 E+ SY+KLP+T+YQI TKFRDE+RPR GL+RGREFLMKDAYSF E LD+ Y R+YKA Sbjct: 121 EVKSYKKLPLTLYQIQTKFRDEQRPRFGLLRGREFLMKDAYSFHATQESLDEVYDRLYKA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEV- 242 Y IF RCGL +RAV AD+GA+GG+ +THEFM +S +GEDT+A DYAAN+E A V Sbjct: 181 YSNIFARCGLNFRAVIADSGAMGGK-DTHEFMVLSDVGEDTIAYSDTSDYAANIEMAPVV 239 Query: 243 --HTEAEAVQRNVSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVL 300 +T+++ ++ A EK TP + I+++ L I ++ +K+++++ K VL Sbjct: 240 ATYTKSDEAEK------ALEKVATPDQKAIEEVSAFLNIEAEKCIKSMVFKVDEKLVVVL 293 Query: 301 VRGDHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAM 360 VRGDHEVN+VKV+N GAS + LA E V+ E G +GP+ + ++ D AVA++ Sbjct: 294 VRGDHEVNDVKVKNVYGASVVELASHEEVKELLNCEVGSLGPIGVTGDIEIIADHAVASI 353 Query: 361 TSGITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIF 420 +G +GA E FH +V P RDF + D R +EG+ P G G + F +GIE+GH+F Sbjct: 354 VNGCSGANEEGFHYVNVNPERDFKVSQYTDLRFIQEGDQSP-DGNGTILFARGIEVGHVF 412 Query: 421 KLGTKYSEKLGASVLDANGRSKLMIMGCYGIGISRLVXXXXLQW 464 KLGT+YSE + A+ LD NG+++ +IMGCYGIG+SR V Q+ Sbjct: 413 KLGTRYSEAMNATFLDENGKTQPLIMGCYGIGVSRTVAAIAEQF 456 >ref|NP_243285.1| prolyl-tRNA synthetase [Bacillus halodurans] pir||C83952 prolyl-tRNA synthetase proS [imported] - Bacillus halodurans (strain C-125) dbj|BAB06138.1| prolyl-tRNA synthetase [Bacillus halodurans] Length = 569 Score = 489 bits (1259), Expect = e-137, Method: Compositional matrix adjust. Identities = 243/454 (53%), Positives = 322/454 (70%), Gaps = 6/454 (1%) Query: 4 MRQSHLLATTLREAPAEAETVNHQLLLRAGYIRQLAAGIYTYLPLGRKVLRKIEAIVREE 63 M+QS L T+RE PA+A+ V+HQL+LRAG IRQ+AAGIYTYLPL R+V+RKIE+IVREE Sbjct: 1 MKQSFFLVPTMREVPADADVVSHQLMLRAGMIRQVAAGIYTYLPLARRVIRKIESIVREE 60 Query: 64 MDAAGAQELLMPSMQPAELWKESERYEVYGKELMKLSDRHDREFVLGPTHEEVITSLMRG 123 +D GAQE+ MP++ PAELW+ES R+E YG ELM+L+DRH+R+F LGPTHEEVITSL+R Sbjct: 61 LDQTGAQEITMPTLHPAELWQESGRWEKYGDELMRLTDRHNRQFALGPTHEEVITSLIRD 120 Query: 124 EISSYRKLPITVYQIGTKFRDERRPRSGLMRGREFLMKDAYSFDTGWEGLDDTYSRMYKA 183 I+SY+KLP+ VYQI +KFRDERRPR GL+RGREF+MKDAYSF TG E LD+ Y MY A Sbjct: 121 SINSYKKLPLNVYQIQSKFRDERRPRFGLLRGREFIMKDAYSFHTGGESLDEMYKVMYDA 180 Query: 184 YQRIFERCGLVYRAVHADAGAIGGQGETHEFMAISGIGEDTVAVCSACDYAANLEQAEVH 243 Y RIF R GL R V AD+GAIGG+ +THEFMA++ +GEDT+A DYAAN+E A V Sbjct: 181 YSRIFRRAGLNVRPVIADSGAIGGK-DTHEFMALADVGEDTIAYSDQSDYAANIEMAAVK 239 Query: 244 TEAEAVQRNVSNLPAAEKFHTPGLRTIDQLEQELQIAPDQVMKTLIYQGGGKTFAVLVRG 303 R L E T +TI + + L + ++++K+L+ + +L+RG Sbjct: 240 VN---YTRPDEPLLERELVDTGDAKTIRAVAERLSVQEEKIIKSLLVSIDDEWALILLRG 296 Query: 304 DHEVNEVKVRNYIGASEIALADSETVQAAAFVESGYVGPVXXXXPVTLLVDRAVAAMTSG 363 DHE+N++K+++ +GAS+I LA E V E G +GPV V ++ D + ++ +G Sbjct: 297 DHELNDIKLKHALGASDIRLATEEEVLEVIGTEVGTIGPVDVKG-VKVIADHGIKSIVNG 355 Query: 364 ITGAGERDFHLRDVVPGRDFALEHVADFRTAEEGETCPVCGKGVLHFQQGIEIGHIFKLG 423 + GA E + H V RDF ++ AD R +EG+ P GKG + F QGIE+G +FKLG Sbjct: 356 VCGANESNKHFIHVNEERDFTVDTYADLRFIQEGDPSP-DGKGTIRFAQGIEVGQVFKLG 414 Query: 424 TKYSEKLGASVLDANGRSKLMIMGCYGIGISRLV 457 T YSEKLGA+ LD NG+S+ M+MGCYGIG+SR+V Sbjct: 415 TVYSEKLGATFLDENGKSQPMLMGCYGIGVSRMV 448 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 673,698,497 Number of extensions: 27138994 Number of successful extensions: 66493 Number of sequences better than 1.0e-05: 900 Number of HSP's gapped: 65754 Number of HSP's successfully gapped: 1030 Length of query: 488 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 358 Effective length of database: 404,274,708 Effective search space: 144730345464 Effective search space used: 144730345464 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)